Effect of drought stress on symbiotic nitrogen fixation, soil nitrogen availability and soil microbial diversity in forage legumes
Background and aims Forage legumes form mutualistic interactions with specialized soil rhizobia bacteria that inhabit root nodules and fix atmospheric nitrogen. However, legumes are sensitive to drought stress, which can interrupt nodulation and symbiotic nitrogen fixation (SNF). We hypothesize that...
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| Veröffentlicht in: | Plant and soil 2024-02, Vol.495 (1-2), p.445-467 |
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| creator | Dollete, Danielito Lumactud, Rhea Amor Carlyle, Cameron N. Szczyglowski, Krzysztof Hill, Brett Thilakarathna, Malinda S. |
| description | Background and aims
Forage legumes form mutualistic interactions with specialized soil rhizobia bacteria that inhabit root nodules and fix atmospheric nitrogen. However, legumes are sensitive to drought stress, which can interrupt nodulation and symbiotic nitrogen fixation (SNF). We hypothesize that drought-impaired SNF may influence soil nitrogen availability and soil microbial diversity.
Methods
Here, we evaluated the effects of drought on nodulation, plant growth, physiological parameters, SNF, soil nitrogen availability, soil extracellular enzyme activity, and soil microbiome of alfalfa (
Medicago sativa
) and red clover (
Trifolium pratense
). The drought treatments were imposed at the flowering stage by maintaining soil moisture contents at 20% field capacity (FC) (severe drought), 40% FC (moderate drought), and 80% FC (well-watered) for three weeks.
Results
Drought significantly reduced nodulation, root and shoot growth, and SNF in alfalfa and red clover. Soil available nitrogen was significantly increased following severe drought conditions. The enzyme assays showed reduced activity of N-acetyl-glucosaminidase and β-D cellobiosidase enzymes under drought stress in alfalfa and red clover, respectively. Microbiome data showed shifts in the relative abundance of some key bacterial taxa under drought stress.
Conclusion
Overall results indicate that drought has deleterious effects on SNF and plant growth, affecting carbon and nitrogen cycling enzymes, soil nitrogen availability, and soil microbial diversity. |
| doi_str_mv | 10.1007/s11104-023-06348-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153620155</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2921361920</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-9c028d8164a6c0e00cc4bd79a3abda08b569d169b4ae367b1687aabb922b30f03</originalsourceid><addsrcrecordid>eNp9kc1q3TAQhUVpILdJXyArQTdd1M1IsmV7WUJ-CoFsUuhOjGTZVbCtVCOH3m2fPL51IJBFV8PMnPMxw2HsTMBXAVCfkxACygKkKkCrsinEO7YTVa2KCpR-z3YAShZQtz-P2QeiBzj0Qu_Y38u-9y7z2PMuxWX4lTnl5Il4nDntJxtiDo7PIac4-Jn34Q_mEOcvnGIYX-f4hGFEG8aQ9xznbltPwaVoA468C08-0WEZVkhMOHg--mGZPJ2yox5H8h9f6gn7cXV5f3FT3N5df7_4dls4VclctA5k0zVCl6gdeADnStvVLSq0HUJjK912Qre2RK90bYVuakRrWymtgh7UCfu8cR9T_L14ymYK5Pw44uzjQkaJSmkJoqpW6ac30oe4pHm9zshWCqVFKw9AuanWJ4mS781jChOmvRFgDrGYLRazxmL-xWLEalKbiVbxPPj0iv6P6xmqzJJe</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2921361920</pqid></control><display><type>article</type><title>Effect of drought stress on symbiotic nitrogen fixation, soil nitrogen availability and soil microbial diversity in forage legumes</title><source>SpringerLink Journals - AutoHoldings</source><creator>Dollete, Danielito ; Lumactud, Rhea Amor ; Carlyle, Cameron N. ; Szczyglowski, Krzysztof ; Hill, Brett ; Thilakarathna, Malinda S.</creator><creatorcontrib>Dollete, Danielito ; Lumactud, Rhea Amor ; Carlyle, Cameron N. ; Szczyglowski, Krzysztof ; Hill, Brett ; Thilakarathna, Malinda S.</creatorcontrib><description>Background and aims
Forage legumes form mutualistic interactions with specialized soil rhizobia bacteria that inhabit root nodules and fix atmospheric nitrogen. However, legumes are sensitive to drought stress, which can interrupt nodulation and symbiotic nitrogen fixation (SNF). We hypothesize that drought-impaired SNF may influence soil nitrogen availability and soil microbial diversity.
Methods
Here, we evaluated the effects of drought on nodulation, plant growth, physiological parameters, SNF, soil nitrogen availability, soil extracellular enzyme activity, and soil microbiome of alfalfa (
Medicago sativa
) and red clover (
Trifolium pratense
). The drought treatments were imposed at the flowering stage by maintaining soil moisture contents at 20% field capacity (FC) (severe drought), 40% FC (moderate drought), and 80% FC (well-watered) for three weeks.
Results
Drought significantly reduced nodulation, root and shoot growth, and SNF in alfalfa and red clover. Soil available nitrogen was significantly increased following severe drought conditions. The enzyme assays showed reduced activity of N-acetyl-glucosaminidase and β-D cellobiosidase enzymes under drought stress in alfalfa and red clover, respectively. Microbiome data showed shifts in the relative abundance of some key bacterial taxa under drought stress.
Conclusion
Overall results indicate that drought has deleterious effects on SNF and plant growth, affecting carbon and nitrogen cycling enzymes, soil nitrogen availability, and soil microbial diversity.</description><identifier>ISSN: 0032-079X</identifier><identifier>EISSN: 1573-5036</identifier><identifier>DOI: 10.1007/s11104-023-06348-1</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Agriculture ; Alfalfa ; Availability ; Biomedical and Life Sciences ; carbon ; Carbon cycle ; Clover ; Drought ; Ecology ; Enzymatic activity ; Enzyme activity ; Enzymes ; Field capacity ; Flowering ; forage ; Glucosaminidase ; Legumes ; Life Sciences ; Medicago sativa ; microbiome ; Microbiomes ; Microorganisms ; Moisture content ; Nitrogen ; Nitrogen cycle ; Nitrogen fixation ; Nitrogenation ; Nodulation ; Nodules ; Physiological effects ; Plant growth ; Plant Physiology ; Plant Sciences ; Plants (botany) ; Red clover ; Relative abundance ; Research Article ; Root nodules ; Soil bacteria ; soil enzymes ; Soil microorganisms ; Soil moisture ; Soil Science & Conservation ; soil water ; Trifolium pratense ; water stress</subject><ispartof>Plant and soil, 2024-02, Vol.495 (1-2), p.445-467</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-9c028d8164a6c0e00cc4bd79a3abda08b569d169b4ae367b1687aabb922b30f03</citedby><cites>FETCH-LOGICAL-c352t-9c028d8164a6c0e00cc4bd79a3abda08b569d169b4ae367b1687aabb922b30f03</cites><orcidid>0000-0001-9598-2833</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11104-023-06348-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11104-023-06348-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Dollete, Danielito</creatorcontrib><creatorcontrib>Lumactud, Rhea Amor</creatorcontrib><creatorcontrib>Carlyle, Cameron N.</creatorcontrib><creatorcontrib>Szczyglowski, Krzysztof</creatorcontrib><creatorcontrib>Hill, Brett</creatorcontrib><creatorcontrib>Thilakarathna, Malinda S.</creatorcontrib><title>Effect of drought stress on symbiotic nitrogen fixation, soil nitrogen availability and soil microbial diversity in forage legumes</title><title>Plant and soil</title><addtitle>Plant Soil</addtitle><description>Background and aims
Forage legumes form mutualistic interactions with specialized soil rhizobia bacteria that inhabit root nodules and fix atmospheric nitrogen. However, legumes are sensitive to drought stress, which can interrupt nodulation and symbiotic nitrogen fixation (SNF). We hypothesize that drought-impaired SNF may influence soil nitrogen availability and soil microbial diversity.
Methods
Here, we evaluated the effects of drought on nodulation, plant growth, physiological parameters, SNF, soil nitrogen availability, soil extracellular enzyme activity, and soil microbiome of alfalfa (
Medicago sativa
) and red clover (
Trifolium pratense
). The drought treatments were imposed at the flowering stage by maintaining soil moisture contents at 20% field capacity (FC) (severe drought), 40% FC (moderate drought), and 80% FC (well-watered) for three weeks.
Results
Drought significantly reduced nodulation, root and shoot growth, and SNF in alfalfa and red clover. Soil available nitrogen was significantly increased following severe drought conditions. The enzyme assays showed reduced activity of N-acetyl-glucosaminidase and β-D cellobiosidase enzymes under drought stress in alfalfa and red clover, respectively. Microbiome data showed shifts in the relative abundance of some key bacterial taxa under drought stress.
Conclusion
Overall results indicate that drought has deleterious effects on SNF and plant growth, affecting carbon and nitrogen cycling enzymes, soil nitrogen availability, and soil microbial diversity.</description><subject>Agriculture</subject><subject>Alfalfa</subject><subject>Availability</subject><subject>Biomedical and Life Sciences</subject><subject>carbon</subject><subject>Carbon cycle</subject><subject>Clover</subject><subject>Drought</subject><subject>Ecology</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Field capacity</subject><subject>Flowering</subject><subject>forage</subject><subject>Glucosaminidase</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Medicago sativa</subject><subject>microbiome</subject><subject>Microbiomes</subject><subject>Microorganisms</subject><subject>Moisture content</subject><subject>Nitrogen</subject><subject>Nitrogen cycle</subject><subject>Nitrogen fixation</subject><subject>Nitrogenation</subject><subject>Nodulation</subject><subject>Nodules</subject><subject>Physiological effects</subject><subject>Plant growth</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plants (botany)</subject><subject>Red clover</subject><subject>Relative abundance</subject><subject>Research Article</subject><subject>Root nodules</subject><subject>Soil bacteria</subject><subject>soil enzymes</subject><subject>Soil microorganisms</subject><subject>Soil moisture</subject><subject>Soil Science & Conservation</subject><subject>soil water</subject><subject>Trifolium pratense</subject><subject>water stress</subject><issn>0032-079X</issn><issn>1573-5036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kc1q3TAQhUVpILdJXyArQTdd1M1IsmV7WUJ-CoFsUuhOjGTZVbCtVCOH3m2fPL51IJBFV8PMnPMxw2HsTMBXAVCfkxACygKkKkCrsinEO7YTVa2KCpR-z3YAShZQtz-P2QeiBzj0Qu_Y38u-9y7z2PMuxWX4lTnl5Il4nDntJxtiDo7PIac4-Jn34Q_mEOcvnGIYX-f4hGFEG8aQ9xznbltPwaVoA468C08-0WEZVkhMOHg--mGZPJ2yox5H8h9f6gn7cXV5f3FT3N5df7_4dls4VclctA5k0zVCl6gdeADnStvVLSq0HUJjK912Qre2RK90bYVuakRrWymtgh7UCfu8cR9T_L14ymYK5Pw44uzjQkaJSmkJoqpW6ac30oe4pHm9zshWCqVFKw9AuanWJ4mS781jChOmvRFgDrGYLRazxmL-xWLEalKbiVbxPPj0iv6P6xmqzJJe</recordid><startdate>20240201</startdate><enddate>20240201</enddate><creator>Dollete, Danielito</creator><creator>Lumactud, Rhea Amor</creator><creator>Carlyle, Cameron N.</creator><creator>Szczyglowski, Krzysztof</creator><creator>Hill, Brett</creator><creator>Thilakarathna, Malinda S.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7ST</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-9598-2833</orcidid></search><sort><creationdate>20240201</creationdate><title>Effect of drought stress on symbiotic nitrogen fixation, soil nitrogen availability and soil microbial diversity in forage legumes</title><author>Dollete, Danielito ; Lumactud, Rhea Amor ; Carlyle, Cameron N. ; Szczyglowski, Krzysztof ; Hill, Brett ; Thilakarathna, Malinda S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-9c028d8164a6c0e00cc4bd79a3abda08b569d169b4ae367b1687aabb922b30f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Agriculture</topic><topic>Alfalfa</topic><topic>Availability</topic><topic>Biomedical and Life Sciences</topic><topic>carbon</topic><topic>Carbon cycle</topic><topic>Clover</topic><topic>Drought</topic><topic>Ecology</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Field capacity</topic><topic>Flowering</topic><topic>forage</topic><topic>Glucosaminidase</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Medicago sativa</topic><topic>microbiome</topic><topic>Microbiomes</topic><topic>Microorganisms</topic><topic>Moisture content</topic><topic>Nitrogen</topic><topic>Nitrogen cycle</topic><topic>Nitrogen fixation</topic><topic>Nitrogenation</topic><topic>Nodulation</topic><topic>Nodules</topic><topic>Physiological effects</topic><topic>Plant growth</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plants (botany)</topic><topic>Red clover</topic><topic>Relative abundance</topic><topic>Research Article</topic><topic>Root nodules</topic><topic>Soil bacteria</topic><topic>soil enzymes</topic><topic>Soil microorganisms</topic><topic>Soil moisture</topic><topic>Soil Science & Conservation</topic><topic>soil water</topic><topic>Trifolium pratense</topic><topic>water stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dollete, Danielito</creatorcontrib><creatorcontrib>Lumactud, Rhea Amor</creatorcontrib><creatorcontrib>Carlyle, Cameron N.</creatorcontrib><creatorcontrib>Szczyglowski, Krzysztof</creatorcontrib><creatorcontrib>Hill, Brett</creatorcontrib><creatorcontrib>Thilakarathna, Malinda S.</creatorcontrib><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant and soil</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dollete, Danielito</au><au>Lumactud, Rhea Amor</au><au>Carlyle, Cameron N.</au><au>Szczyglowski, Krzysztof</au><au>Hill, Brett</au><au>Thilakarathna, Malinda S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of drought stress on symbiotic nitrogen fixation, soil nitrogen availability and soil microbial diversity in forage legumes</atitle><jtitle>Plant and soil</jtitle><stitle>Plant Soil</stitle><date>2024-02-01</date><risdate>2024</risdate><volume>495</volume><issue>1-2</issue><spage>445</spage><epage>467</epage><pages>445-467</pages><issn>0032-079X</issn><eissn>1573-5036</eissn><abstract>Background and aims
Forage legumes form mutualistic interactions with specialized soil rhizobia bacteria that inhabit root nodules and fix atmospheric nitrogen. However, legumes are sensitive to drought stress, which can interrupt nodulation and symbiotic nitrogen fixation (SNF). We hypothesize that drought-impaired SNF may influence soil nitrogen availability and soil microbial diversity.
Methods
Here, we evaluated the effects of drought on nodulation, plant growth, physiological parameters, SNF, soil nitrogen availability, soil extracellular enzyme activity, and soil microbiome of alfalfa (
Medicago sativa
) and red clover (
Trifolium pratense
). The drought treatments were imposed at the flowering stage by maintaining soil moisture contents at 20% field capacity (FC) (severe drought), 40% FC (moderate drought), and 80% FC (well-watered) for three weeks.
Results
Drought significantly reduced nodulation, root and shoot growth, and SNF in alfalfa and red clover. Soil available nitrogen was significantly increased following severe drought conditions. The enzyme assays showed reduced activity of N-acetyl-glucosaminidase and β-D cellobiosidase enzymes under drought stress in alfalfa and red clover, respectively. Microbiome data showed shifts in the relative abundance of some key bacterial taxa under drought stress.
Conclusion
Overall results indicate that drought has deleterious effects on SNF and plant growth, affecting carbon and nitrogen cycling enzymes, soil nitrogen availability, and soil microbial diversity.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s11104-023-06348-1</doi><tpages>23</tpages><orcidid>https://orcid.org/0000-0001-9598-2833</orcidid></addata></record> |
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| subjects | Agriculture Alfalfa Availability Biomedical and Life Sciences carbon Carbon cycle Clover Drought Ecology Enzymatic activity Enzyme activity Enzymes Field capacity Flowering forage Glucosaminidase Legumes Life Sciences Medicago sativa microbiome Microbiomes Microorganisms Moisture content Nitrogen Nitrogen cycle Nitrogen fixation Nitrogenation Nodulation Nodules Physiological effects Plant growth Plant Physiology Plant Sciences Plants (botany) Red clover Relative abundance Research Article Root nodules Soil bacteria soil enzymes Soil microorganisms Soil moisture Soil Science & Conservation soil water Trifolium pratense water stress |
| title | Effect of drought stress on symbiotic nitrogen fixation, soil nitrogen availability and soil microbial diversity in forage legumes |
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