Effects of Ca2+ signal on the activities of key enzymes and expression of related genes in yeast ethanol metabolism and mitochondrial function during high sugar fermentation
BACKGROUND During high sugar fermentation, yeast is mainly affected by high sugar stress in the early stage. It becomes jointly affected by high sugar and ethanol stress as ethanol accumulates during fermentation. Ca2+, as the second messenger of the cell, mediates various metabolic processes. In th...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2024-07, Vol.104 (9), p.5077-5088 |
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| container_end_page | 5088 |
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| container_issue | 9 |
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| container_title | Journal of the science of food and agriculture |
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| creator | Xie, Dongdong Zheng, Jiaxin Sun, Yingqi Li, Xing Ren, Shuncheng |
| description | BACKGROUND
During high sugar fermentation, yeast is mainly affected by high sugar stress in the early stage. It becomes jointly affected by high sugar and ethanol stress as ethanol accumulates during fermentation. Ca2+, as the second messenger of the cell, mediates various metabolic processes. In this study, the effects of the Ca2+ signal on the activities of key enzymes, expression of related genes of ethanol metabolism, and mitochondrial function were investigated.
RESULTS
The results showed a significant increase in the activities of enzymes related to ethanol metabolism in yeast cells under a high sugar environment. Ca2+ significantly promoted the activities of enzymes related to mitochondrial respiratory metabolism and regulated the carbon flow between ethanol metabolism and the tricarboxylic acid cycle. The high sugar environment affected the expression of genes related to carbon metabolism, while the addition of Ca2+ stabilized the expression of related genes.
CONCLUSION
Ca2+ signal participated in ethanol and mitochondrial metabolism and regulated the key enzymes and related gene expression to enhance the resistance of yeast to stress during high sugar fermentation. © 2024 Society of Chemical Industry. |
| doi_str_mv | 10.1002/jsfa.13341 |
| format | Article |
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During high sugar fermentation, yeast is mainly affected by high sugar stress in the early stage. It becomes jointly affected by high sugar and ethanol stress as ethanol accumulates during fermentation. Ca2+, as the second messenger of the cell, mediates various metabolic processes. In this study, the effects of the Ca2+ signal on the activities of key enzymes, expression of related genes of ethanol metabolism, and mitochondrial function were investigated.
RESULTS
The results showed a significant increase in the activities of enzymes related to ethanol metabolism in yeast cells under a high sugar environment. Ca2+ significantly promoted the activities of enzymes related to mitochondrial respiratory metabolism and regulated the carbon flow between ethanol metabolism and the tricarboxylic acid cycle. The high sugar environment affected the expression of genes related to carbon metabolism, while the addition of Ca2+ stabilized the expression of related genes.
CONCLUSION
Ca2+ signal participated in ethanol and mitochondrial metabolism and regulated the key enzymes and related gene expression to enhance the resistance of yeast to stress during high sugar fermentation. © 2024 Society of Chemical Industry.</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.13341</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>agriculture ; calcium ; Calcium ions ; calcium signal ; Calcium signalling ; Carbon ; carbon metabolism ; enzyme activities ; Enzymes ; Ethanol ; ethanol metabolism ; Fermentation ; Gene expression ; Genes ; Metabolism ; Mitochondria ; mitochondrial function ; Sugar ; sugars ; Tricarboxylic acid cycle ; Yeast ; Yeasts</subject><ispartof>Journal of the science of food and agriculture, 2024-07, Vol.104 (9), p.5077-5088</ispartof><rights>2024 Society of Chemical Industry.</rights><rights>2024 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-6376-5252</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.13341$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.13341$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Xie, Dongdong</creatorcontrib><creatorcontrib>Zheng, Jiaxin</creatorcontrib><creatorcontrib>Sun, Yingqi</creatorcontrib><creatorcontrib>Li, Xing</creatorcontrib><creatorcontrib>Ren, Shuncheng</creatorcontrib><title>Effects of Ca2+ signal on the activities of key enzymes and expression of related genes in yeast ethanol metabolism and mitochondrial function during high sugar fermentation</title><title>Journal of the science of food and agriculture</title><description>BACKGROUND
During high sugar fermentation, yeast is mainly affected by high sugar stress in the early stage. It becomes jointly affected by high sugar and ethanol stress as ethanol accumulates during fermentation. Ca2+, as the second messenger of the cell, mediates various metabolic processes. In this study, the effects of the Ca2+ signal on the activities of key enzymes, expression of related genes of ethanol metabolism, and mitochondrial function were investigated.
RESULTS
The results showed a significant increase in the activities of enzymes related to ethanol metabolism in yeast cells under a high sugar environment. Ca2+ significantly promoted the activities of enzymes related to mitochondrial respiratory metabolism and regulated the carbon flow between ethanol metabolism and the tricarboxylic acid cycle. The high sugar environment affected the expression of genes related to carbon metabolism, while the addition of Ca2+ stabilized the expression of related genes.
CONCLUSION
Ca2+ signal participated in ethanol and mitochondrial metabolism and regulated the key enzymes and related gene expression to enhance the resistance of yeast to stress during high sugar fermentation. © 2024 Society of Chemical Industry.</description><subject>agriculture</subject><subject>calcium</subject><subject>Calcium ions</subject><subject>calcium signal</subject><subject>Calcium signalling</subject><subject>Carbon</subject><subject>carbon metabolism</subject><subject>enzyme activities</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>ethanol metabolism</subject><subject>Fermentation</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Metabolism</subject><subject>Mitochondria</subject><subject>mitochondrial function</subject><subject>Sugar</subject><subject>sugars</subject><subject>Tricarboxylic acid cycle</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAQhi1EJZaWC09giQsSSvHYsbM5VqsWiipxoJwj73qceEnsxXYK4Z36jvVuOXHhNDP6v_lHmp-Qt8AugTH-cZ-svgQhanhBVsDapmIM2EuyKiKvJNT8FXmd0p4x1rZKrcjjtbW4y4kGSzeaf6DJ9V6PNHiaB6R6l92Dyw5PwA9cKPo_y1RG7Q3F34eIKbkCFzXiqDMa2qMvuvN0QZ0yxTxoH0Y6YdbbMLo0nXYnl8NuCN5EV87Z2ZdLxcfM0fmeDq4faJp7HanFOKHP-ihfkDOrx4Rv_tZz8v3m-n7zubr7-ul2c3VXHTgHqEwNVq6ZUqZmvNZQM6lQoN2isUKuJZp2q4S0aluvdSsZV3YthW1RWCNUA-KcvH_2PcTwc8aUu8mlHY6j9hjm1AmQQgEoJf-L8hbappZcNQV99w-6D3Ms3y6GTDVKMNEcDeGZ-uVGXLpDdJOOSwesOybcHRPuTgl3X77dXJ068QT2pp2Q</recordid><startdate>202407</startdate><enddate>202407</enddate><creator>Xie, Dongdong</creator><creator>Zheng, Jiaxin</creator><creator>Sun, Yingqi</creator><creator>Li, Xing</creator><creator>Ren, Shuncheng</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-6376-5252</orcidid></search><sort><creationdate>202407</creationdate><title>Effects of Ca2+ signal on the activities of key enzymes and expression of related genes in yeast ethanol metabolism and mitochondrial function during high sugar fermentation</title><author>Xie, Dongdong ; Zheng, Jiaxin ; Sun, Yingqi ; Li, Xing ; Ren, Shuncheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2211-d41f58066d4024a14056e3efbedf3585ed9b635f6b48a95026f853f9e3fd36713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>agriculture</topic><topic>calcium</topic><topic>Calcium ions</topic><topic>calcium signal</topic><topic>Calcium signalling</topic><topic>Carbon</topic><topic>carbon metabolism</topic><topic>enzyme activities</topic><topic>Enzymes</topic><topic>Ethanol</topic><topic>ethanol metabolism</topic><topic>Fermentation</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Metabolism</topic><topic>Mitochondria</topic><topic>mitochondrial function</topic><topic>Sugar</topic><topic>sugars</topic><topic>Tricarboxylic acid cycle</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Dongdong</creatorcontrib><creatorcontrib>Zheng, Jiaxin</creatorcontrib><creatorcontrib>Sun, Yingqi</creatorcontrib><creatorcontrib>Li, Xing</creatorcontrib><creatorcontrib>Ren, Shuncheng</creatorcontrib><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Dongdong</au><au>Zheng, Jiaxin</au><au>Sun, Yingqi</au><au>Li, Xing</au><au>Ren, Shuncheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Ca2+ signal on the activities of key enzymes and expression of related genes in yeast ethanol metabolism and mitochondrial function during high sugar fermentation</atitle><jtitle>Journal of the science of food and agriculture</jtitle><date>2024-07</date><risdate>2024</risdate><volume>104</volume><issue>9</issue><spage>5077</spage><epage>5088</epage><pages>5077-5088</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><abstract>BACKGROUND
During high sugar fermentation, yeast is mainly affected by high sugar stress in the early stage. It becomes jointly affected by high sugar and ethanol stress as ethanol accumulates during fermentation. Ca2+, as the second messenger of the cell, mediates various metabolic processes. In this study, the effects of the Ca2+ signal on the activities of key enzymes, expression of related genes of ethanol metabolism, and mitochondrial function were investigated.
RESULTS
The results showed a significant increase in the activities of enzymes related to ethanol metabolism in yeast cells under a high sugar environment. Ca2+ significantly promoted the activities of enzymes related to mitochondrial respiratory metabolism and regulated the carbon flow between ethanol metabolism and the tricarboxylic acid cycle. The high sugar environment affected the expression of genes related to carbon metabolism, while the addition of Ca2+ stabilized the expression of related genes.
CONCLUSION
Ca2+ signal participated in ethanol and mitochondrial metabolism and regulated the key enzymes and related gene expression to enhance the resistance of yeast to stress during high sugar fermentation. © 2024 Society of Chemical Industry.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jsfa.13341</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-6376-5252</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | agriculture calcium Calcium ions calcium signal Calcium signalling Carbon carbon metabolism enzyme activities Enzymes Ethanol ethanol metabolism Fermentation Gene expression Genes Metabolism Mitochondria mitochondrial function Sugar sugars Tricarboxylic acid cycle Yeast Yeasts |
| title | Effects of Ca2+ signal on the activities of key enzymes and expression of related genes in yeast ethanol metabolism and mitochondrial function during high sugar fermentation |
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