Optimization and mechanism analysis of photosynthetic EPA production in Nannochloropsis salina: Evaluating the effect of temperature and nitrogen concentrations
Microalgae, recognized as sustainable and eco-friendly photosynthetic microorganisms, play a pivotal role in converting CO2 into value-added products. Among these, Nannochloropsis salina (Microchloropsis salina) stands out, particularly for its ability to produce eicosapentaenoic acid (EPA), a cruci...
Gespeichert in:
| Veröffentlicht in: | Plant physiology and biochemistry 2024-06, Vol.211, p.108729-108729, Article 108729 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 108729 |
|---|---|
| container_issue | |
| container_start_page | 108729 |
| container_title | Plant physiology and biochemistry |
| container_volume | 211 |
| creator | Koh, Hyun Gi Jeon, Seungjib Kim, Minsik Chang, Yong Keun Park, Kyungmoon Park, See-Hyoung Kang, Nam Kyu |
| description | Microalgae, recognized as sustainable and eco-friendly photosynthetic microorganisms, play a pivotal role in converting CO2 into value-added products. Among these, Nannochloropsis salina (Microchloropsis salina) stands out, particularly for its ability to produce eicosapentaenoic acid (EPA), a crucial omega-3 fatty acid with significant health benefits such as anti-inflammatory properties and cardiovascular health promotion. This study focused on optimizing the cultivation conditions of Nannochloropsis salina to maximize EPA production. We thoroughly investigated the effects of varying temperatures and nitrogen (NaNO3) concentrations on biomass, total lipid content, and EPA proportions. We successfully identified optimal conditions at an initial NaNO3 concentration of 1.28 g.L−1 and a temperature of 21 °C. This condition was further validated by response surface methodology, which resulted in the highest EPA productivity reported in batch systems (14.4 mg.L−1.day−1). Quantitative real-time PCR and transcriptomic analysis also demonstrated a positive correlation between specific gene expressions and enhanced EPA production. Through a comprehensive lipid analysis and photosynthetic pigment analysis, we deduced that the production of EPA in Nannochloropsis salina seemed to be produced by the remodeling of chloroplast membrane lipids. These findings provide crucial insights into how temperature and nutrient availability influence fatty acid composition in N. salina, offering valuable guidance for developing strategies to improve EPA production in various microalgae species.
[Display omitted]
•NaNO3 and temperature conditions were optimized for EPA production in N. salina.•Lipid and pigment analysis indicate EPA remodeling in chloroplast membranes.•A positive correlation was found between gene expression and EPA production.•Response surface methodology confirms ideal conditions for EPA synthesis. |
| doi_str_mv | 10.1016/j.plaphy.2024.108729 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153609897</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0981942824003978</els_id><sourcerecordid>3056668750</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-4033fdc284af0d3989b189e8ebcf385cfcdaaab305fc1b9866ffeaa71de6fb63</originalsourceid><addsrcrecordid>eNqFkc1u3CAUhVHVqpkmfYOqYtmNp2BsjLuoFEXTHylqssgeYXyJGdngAo40eZo-aplx2mWyQqDvnHs5B6EPlGwpofzzfjuPah4O25KUVX4STdm-QhsqGlaUvCWv0Ya0ghZtVYoz9C7GPSGZbNhbdMZEU1e0aTboz82c7GQfVbLeYeV6PIEelLNxyjc1HqKN2Bs8Dz75eHBpgGQ13t1e4jn4ftEnnXX4l3LO62H0wc9HTVSjdeoL3j2occnu7h5nLQZjQKejY4JphqDSEuA019kU_D04rL3T4FI4rRQv0Bujxgjvn85zdPdtd3f1o7i--f7z6vK60KyqUlERxkyvS1EpQ3rWirajogUBnTZM1NroXinVMVIbTbtWcJ4XUaqhPXDTcXaOPq22-Ve_F4hJTjZqGEflwC9RMloznvNsm5dRUnPOc8Iko9WK6uBjDGDkHOykwkFSIo8tyr1cW5THFuXaYpZ9fJqwdBP0_0X_asvA1xWAnMiDhSCjtpBj623I8cre2-cn_AVAPrXY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3056668750</pqid></control><display><type>article</type><title>Optimization and mechanism analysis of photosynthetic EPA production in Nannochloropsis salina: Evaluating the effect of temperature and nitrogen concentrations</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Koh, Hyun Gi ; Jeon, Seungjib ; Kim, Minsik ; Chang, Yong Keun ; Park, Kyungmoon ; Park, See-Hyoung ; Kang, Nam Kyu</creator><creatorcontrib>Koh, Hyun Gi ; Jeon, Seungjib ; Kim, Minsik ; Chang, Yong Keun ; Park, Kyungmoon ; Park, See-Hyoung ; Kang, Nam Kyu</creatorcontrib><description>Microalgae, recognized as sustainable and eco-friendly photosynthetic microorganisms, play a pivotal role in converting CO2 into value-added products. Among these, Nannochloropsis salina (Microchloropsis salina) stands out, particularly for its ability to produce eicosapentaenoic acid (EPA), a crucial omega-3 fatty acid with significant health benefits such as anti-inflammatory properties and cardiovascular health promotion. This study focused on optimizing the cultivation conditions of Nannochloropsis salina to maximize EPA production. We thoroughly investigated the effects of varying temperatures and nitrogen (NaNO3) concentrations on biomass, total lipid content, and EPA proportions. We successfully identified optimal conditions at an initial NaNO3 concentration of 1.28 g.L−1 and a temperature of 21 °C. This condition was further validated by response surface methodology, which resulted in the highest EPA productivity reported in batch systems (14.4 mg.L−1.day−1). Quantitative real-time PCR and transcriptomic analysis also demonstrated a positive correlation between specific gene expressions and enhanced EPA production. Through a comprehensive lipid analysis and photosynthetic pigment analysis, we deduced that the production of EPA in Nannochloropsis salina seemed to be produced by the remodeling of chloroplast membrane lipids. These findings provide crucial insights into how temperature and nutrient availability influence fatty acid composition in N. salina, offering valuable guidance for developing strategies to improve EPA production in various microalgae species.
[Display omitted]
•NaNO3 and temperature conditions were optimized for EPA production in N. salina.•Lipid and pigment analysis indicate EPA remodeling in chloroplast membranes.•A positive correlation was found between gene expression and EPA production.•Response surface methodology confirms ideal conditions for EPA synthesis.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2024.108729</identifier><identifier>PMID: 38754177</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Biomass ; carbon dioxide ; chloroplast membranes ; Culture optimization ; Eicosapentaenoic acid ; Eicosapentaenoic Acid - biosynthesis ; Eicosapentaenoic Acid - metabolism ; EPA ; fatty acid composition ; genes ; health promotion ; lipid content ; Microalgae ; Microalgae - metabolism ; Nannochloropsis ; Nannochloropsis salina ; nitrogen ; Nitrogen - metabolism ; nutrient availability ; Omega-3 ; omega-3 fatty acids ; Photosynthesis ; plant physiology ; quantitative polymerase chain reaction ; response surface methodology ; RSM ; species ; Stramenopiles - genetics ; Stramenopiles - metabolism ; Temperature ; transcriptomics ; value added</subject><ispartof>Plant physiology and biochemistry, 2024-06, Vol.211, p.108729-108729, Article 108729</ispartof><rights>2024 Elsevier Masson SAS</rights><rights>Copyright © 2024 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c344t-4033fdc284af0d3989b189e8ebcf385cfcdaaab305fc1b9866ffeaa71de6fb63</cites><orcidid>0000-0002-1348-1312 ; 0000-0003-0223-4882 ; 0000-0003-1095-840X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0981942824003978$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38754177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koh, Hyun Gi</creatorcontrib><creatorcontrib>Jeon, Seungjib</creatorcontrib><creatorcontrib>Kim, Minsik</creatorcontrib><creatorcontrib>Chang, Yong Keun</creatorcontrib><creatorcontrib>Park, Kyungmoon</creatorcontrib><creatorcontrib>Park, See-Hyoung</creatorcontrib><creatorcontrib>Kang, Nam Kyu</creatorcontrib><title>Optimization and mechanism analysis of photosynthetic EPA production in Nannochloropsis salina: Evaluating the effect of temperature and nitrogen concentrations</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>Microalgae, recognized as sustainable and eco-friendly photosynthetic microorganisms, play a pivotal role in converting CO2 into value-added products. Among these, Nannochloropsis salina (Microchloropsis salina) stands out, particularly for its ability to produce eicosapentaenoic acid (EPA), a crucial omega-3 fatty acid with significant health benefits such as anti-inflammatory properties and cardiovascular health promotion. This study focused on optimizing the cultivation conditions of Nannochloropsis salina to maximize EPA production. We thoroughly investigated the effects of varying temperatures and nitrogen (NaNO3) concentrations on biomass, total lipid content, and EPA proportions. We successfully identified optimal conditions at an initial NaNO3 concentration of 1.28 g.L−1 and a temperature of 21 °C. This condition was further validated by response surface methodology, which resulted in the highest EPA productivity reported in batch systems (14.4 mg.L−1.day−1). Quantitative real-time PCR and transcriptomic analysis also demonstrated a positive correlation between specific gene expressions and enhanced EPA production. Through a comprehensive lipid analysis and photosynthetic pigment analysis, we deduced that the production of EPA in Nannochloropsis salina seemed to be produced by the remodeling of chloroplast membrane lipids. These findings provide crucial insights into how temperature and nutrient availability influence fatty acid composition in N. salina, offering valuable guidance for developing strategies to improve EPA production in various microalgae species.
[Display omitted]
•NaNO3 and temperature conditions were optimized for EPA production in N. salina.•Lipid and pigment analysis indicate EPA remodeling in chloroplast membranes.•A positive correlation was found between gene expression and EPA production.•Response surface methodology confirms ideal conditions for EPA synthesis.</description><subject>Biomass</subject><subject>carbon dioxide</subject><subject>chloroplast membranes</subject><subject>Culture optimization</subject><subject>Eicosapentaenoic acid</subject><subject>Eicosapentaenoic Acid - biosynthesis</subject><subject>Eicosapentaenoic Acid - metabolism</subject><subject>EPA</subject><subject>fatty acid composition</subject><subject>genes</subject><subject>health promotion</subject><subject>lipid content</subject><subject>Microalgae</subject><subject>Microalgae - metabolism</subject><subject>Nannochloropsis</subject><subject>Nannochloropsis salina</subject><subject>nitrogen</subject><subject>Nitrogen - metabolism</subject><subject>nutrient availability</subject><subject>Omega-3</subject><subject>omega-3 fatty acids</subject><subject>Photosynthesis</subject><subject>plant physiology</subject><subject>quantitative polymerase chain reaction</subject><subject>response surface methodology</subject><subject>RSM</subject><subject>species</subject><subject>Stramenopiles - genetics</subject><subject>Stramenopiles - metabolism</subject><subject>Temperature</subject><subject>transcriptomics</subject><subject>value added</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u3CAUhVHVqpkmfYOqYtmNp2BsjLuoFEXTHylqssgeYXyJGdngAo40eZo-aplx2mWyQqDvnHs5B6EPlGwpofzzfjuPah4O25KUVX4STdm-QhsqGlaUvCWv0Ya0ghZtVYoz9C7GPSGZbNhbdMZEU1e0aTboz82c7GQfVbLeYeV6PIEelLNxyjc1HqKN2Bs8Dz75eHBpgGQ13t1e4jn4ftEnnXX4l3LO62H0wc9HTVSjdeoL3j2occnu7h5nLQZjQKejY4JphqDSEuA019kU_D04rL3T4FI4rRQv0Bujxgjvn85zdPdtd3f1o7i--f7z6vK60KyqUlERxkyvS1EpQ3rWirajogUBnTZM1NroXinVMVIbTbtWcJ4XUaqhPXDTcXaOPq22-Ve_F4hJTjZqGEflwC9RMloznvNsm5dRUnPOc8Iko9WK6uBjDGDkHOykwkFSIo8tyr1cW5THFuXaYpZ9fJqwdBP0_0X_asvA1xWAnMiDhSCjtpBj623I8cre2-cn_AVAPrXY</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Koh, Hyun Gi</creator><creator>Jeon, Seungjib</creator><creator>Kim, Minsik</creator><creator>Chang, Yong Keun</creator><creator>Park, Kyungmoon</creator><creator>Park, See-Hyoung</creator><creator>Kang, Nam Kyu</creator><general>Elsevier Masson SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-1348-1312</orcidid><orcidid>https://orcid.org/0000-0003-0223-4882</orcidid><orcidid>https://orcid.org/0000-0003-1095-840X</orcidid></search><sort><creationdate>20240601</creationdate><title>Optimization and mechanism analysis of photosynthetic EPA production in Nannochloropsis salina: Evaluating the effect of temperature and nitrogen concentrations</title><author>Koh, Hyun Gi ; Jeon, Seungjib ; Kim, Minsik ; Chang, Yong Keun ; Park, Kyungmoon ; Park, See-Hyoung ; Kang, Nam Kyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-4033fdc284af0d3989b189e8ebcf385cfcdaaab305fc1b9866ffeaa71de6fb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biomass</topic><topic>carbon dioxide</topic><topic>chloroplast membranes</topic><topic>Culture optimization</topic><topic>Eicosapentaenoic acid</topic><topic>Eicosapentaenoic Acid - biosynthesis</topic><topic>Eicosapentaenoic Acid - metabolism</topic><topic>EPA</topic><topic>fatty acid composition</topic><topic>genes</topic><topic>health promotion</topic><topic>lipid content</topic><topic>Microalgae</topic><topic>Microalgae - metabolism</topic><topic>Nannochloropsis</topic><topic>Nannochloropsis salina</topic><topic>nitrogen</topic><topic>Nitrogen - metabolism</topic><topic>nutrient availability</topic><topic>Omega-3</topic><topic>omega-3 fatty acids</topic><topic>Photosynthesis</topic><topic>plant physiology</topic><topic>quantitative polymerase chain reaction</topic><topic>response surface methodology</topic><topic>RSM</topic><topic>species</topic><topic>Stramenopiles - genetics</topic><topic>Stramenopiles - metabolism</topic><topic>Temperature</topic><topic>transcriptomics</topic><topic>value added</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koh, Hyun Gi</creatorcontrib><creatorcontrib>Jeon, Seungjib</creatorcontrib><creatorcontrib>Kim, Minsik</creatorcontrib><creatorcontrib>Chang, Yong Keun</creatorcontrib><creatorcontrib>Park, Kyungmoon</creatorcontrib><creatorcontrib>Park, See-Hyoung</creatorcontrib><creatorcontrib>Kang, Nam Kyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koh, Hyun Gi</au><au>Jeon, Seungjib</au><au>Kim, Minsik</au><au>Chang, Yong Keun</au><au>Park, Kyungmoon</au><au>Park, See-Hyoung</au><au>Kang, Nam Kyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization and mechanism analysis of photosynthetic EPA production in Nannochloropsis salina: Evaluating the effect of temperature and nitrogen concentrations</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>211</volume><spage>108729</spage><epage>108729</epage><pages>108729-108729</pages><artnum>108729</artnum><issn>0981-9428</issn><eissn>1873-2690</eissn><abstract>Microalgae, recognized as sustainable and eco-friendly photosynthetic microorganisms, play a pivotal role in converting CO2 into value-added products. Among these, Nannochloropsis salina (Microchloropsis salina) stands out, particularly for its ability to produce eicosapentaenoic acid (EPA), a crucial omega-3 fatty acid with significant health benefits such as anti-inflammatory properties and cardiovascular health promotion. This study focused on optimizing the cultivation conditions of Nannochloropsis salina to maximize EPA production. We thoroughly investigated the effects of varying temperatures and nitrogen (NaNO3) concentrations on biomass, total lipid content, and EPA proportions. We successfully identified optimal conditions at an initial NaNO3 concentration of 1.28 g.L−1 and a temperature of 21 °C. This condition was further validated by response surface methodology, which resulted in the highest EPA productivity reported in batch systems (14.4 mg.L−1.day−1). Quantitative real-time PCR and transcriptomic analysis also demonstrated a positive correlation between specific gene expressions and enhanced EPA production. Through a comprehensive lipid analysis and photosynthetic pigment analysis, we deduced that the production of EPA in Nannochloropsis salina seemed to be produced by the remodeling of chloroplast membrane lipids. These findings provide crucial insights into how temperature and nutrient availability influence fatty acid composition in N. salina, offering valuable guidance for developing strategies to improve EPA production in various microalgae species.
[Display omitted]
•NaNO3 and temperature conditions were optimized for EPA production in N. salina.•Lipid and pigment analysis indicate EPA remodeling in chloroplast membranes.•A positive correlation was found between gene expression and EPA production.•Response surface methodology confirms ideal conditions for EPA synthesis.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>38754177</pmid><doi>10.1016/j.plaphy.2024.108729</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1348-1312</orcidid><orcidid>https://orcid.org/0000-0003-0223-4882</orcidid><orcidid>https://orcid.org/0000-0003-1095-840X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0981-9428 |
| ispartof | Plant physiology and biochemistry, 2024-06, Vol.211, p.108729-108729, Article 108729 |
| issn | 0981-9428 1873-2690 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153609897 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biomass carbon dioxide chloroplast membranes Culture optimization Eicosapentaenoic acid Eicosapentaenoic Acid - biosynthesis Eicosapentaenoic Acid - metabolism EPA fatty acid composition genes health promotion lipid content Microalgae Microalgae - metabolism Nannochloropsis Nannochloropsis salina nitrogen Nitrogen - metabolism nutrient availability Omega-3 omega-3 fatty acids Photosynthesis plant physiology quantitative polymerase chain reaction response surface methodology RSM species Stramenopiles - genetics Stramenopiles - metabolism Temperature transcriptomics value added |
| title | Optimization and mechanism analysis of photosynthetic EPA production in Nannochloropsis salina: Evaluating the effect of temperature and nitrogen concentrations |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T07%3A35%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Optimization%20and%20mechanism%20analysis%20of%20photosynthetic%20EPA%20production%20in%20Nannochloropsis%20salina:%20Evaluating%20the%20effect%20of%20temperature%20and%20nitrogen%20concentrations&rft.jtitle=Plant%20physiology%20and%20biochemistry&rft.au=Koh,%20Hyun%20Gi&rft.date=2024-06-01&rft.volume=211&rft.spage=108729&rft.epage=108729&rft.pages=108729-108729&rft.artnum=108729&rft.issn=0981-9428&rft.eissn=1873-2690&rft_id=info:doi/10.1016/j.plaphy.2024.108729&rft_dat=%3Cproquest_cross%3E3056668750%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3056668750&rft_id=info:pmid/38754177&rft_els_id=S0981942824003978&rfr_iscdi=true |