Rapid analysis of insecticidal metabolites from the entomopathogenic fungus Beauveria bassiana 331R using UPLC-Q-Orbitrap MS
Beauveria bassiana , a representative entomopathogenic fungus, is increasingly being utilized as an eco-friendly pest management alternative to chemical insecticides. This fungus produces a range of insecticidal secondary metabolites that act as antimicrobial and immunosuppressive agents. However, d...
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| Veröffentlicht in: | Mycotoxin research 2024-02, Vol.40 (1), p.123-132 |
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| creator | Kim, Jong-Cheol Hwang, In Min Kim, Ho Myeong Kim, Seulbi Shin, Teak Su Woo, Soo-Dong Park, Hae Woong |
| description | Beauveria bassiana
, a representative entomopathogenic fungus, is increasingly being utilized as an eco-friendly pest management alternative to chemical insecticides. This fungus produces a range of insecticidal secondary metabolites that act as antimicrobial and immunosuppressive agents. However, detailed qualitative and quantitative analysis related to these compounds remains scarce, we developed a method for the rapid analysis of these metabolites. Eight secondary metabolites (bassianin, bassianolide, beauvericin, beauveriolide I, enniatin A, A1, and B, and tenellin) were efficiently extracted when
B. bassiana
-infected
Tenebrio molitor
larvae were ground in 70% EtOH extraction solvent and subsequently subjected to ultrasonic treatment for 30 min. The eight metabolites were rapidly and simultaneously analyzed using ultra-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). Bassianolide (20.6–51.1 µg/g) and beauvericin (63.6–109.8 µg/g) were identified as the main metabolites in
B. basssiana
-infected larvae, indicating that they are likely major toxins of
B. bassiana
. Validation of the method exhibited recovery rates in the range of 80–115% and precision in the range of 0.1–8.0%, indicating no significant interference from compounds in the matrix. We developed a method to rapidly analyze eight insecticidal metabolites using UPLC-Q-Orbitrap MS. This can be extensively utilized for detecting and producing insecticidal fungal secondary metabolites. |
| doi_str_mv | 10.1007/s12550-023-00509-y |
| format | Article |
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, a representative entomopathogenic fungus, is increasingly being utilized as an eco-friendly pest management alternative to chemical insecticides. This fungus produces a range of insecticidal secondary metabolites that act as antimicrobial and immunosuppressive agents. However, detailed qualitative and quantitative analysis related to these compounds remains scarce, we developed a method for the rapid analysis of these metabolites. Eight secondary metabolites (bassianin, bassianolide, beauvericin, beauveriolide I, enniatin A, A1, and B, and tenellin) were efficiently extracted when
B. bassiana
-infected
Tenebrio molitor
larvae were ground in 70% EtOH extraction solvent and subsequently subjected to ultrasonic treatment for 30 min. The eight metabolites were rapidly and simultaneously analyzed using ultra-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). Bassianolide (20.6–51.1 µg/g) and beauvericin (63.6–109.8 µg/g) were identified as the main metabolites in
B. basssiana
-infected larvae, indicating that they are likely major toxins of
B. bassiana
. Validation of the method exhibited recovery rates in the range of 80–115% and precision in the range of 0.1–8.0%, indicating no significant interference from compounds in the matrix. We developed a method to rapidly analyze eight insecticidal metabolites using UPLC-Q-Orbitrap MS. This can be extensively utilized for detecting and producing insecticidal fungal secondary metabolites.</description><identifier>ISSN: 0178-7888</identifier><identifier>EISSN: 1867-1632</identifier><identifier>DOI: 10.1007/s12550-023-00509-y</identifier><identifier>PMID: 37968430</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Beauveria - metabolism ; Beauveria bassiana ; Beauvericin ; Biomedical and Life Sciences ; Chemistry/Food Science ; Chromatography, High Pressure Liquid ; Entomopathogenic fungi ; Fungi ; immunosuppression ; Immunosuppressive agents ; Insecticides ; Larva ; Larvae ; Life Sciences ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Medical Microbiology ; Medicine/Public Health ; Metabolites ; Microbiology ; mycotoxins ; Original Article ; Pest control ; pest management ; Quadrupoles ; Qualitative analysis ; quantitative analysis ; rapid methods ; Secondary metabolites ; solvents ; Tenebrio ; Tenebrio molitor ; Toxins ; Ultrasonic imaging ; Ultrasonic processing ; ultrasonic treatment</subject><ispartof>Mycotoxin research, 2024-02, Vol.40 (1), p.123-132</ispartof><rights>The Author(s) under exclusive licence to Society for Mycotoxin (Research Gesellschaft für Mykotoxinforschung e.V.) and Springer-Verlag GmbH Germany, part of Springer Nature 2023. corrected publication 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s) under exclusive licence to Society for Mycotoxin (Research Gesellschaft für Mykotoxinforschung e.V.) and Springer-Verlag GmbH Germany, part of Springer Nature.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-8e2f806144c273b945e6ff6bc0cd79ae63494b8079d258215e4eee4bc4fc7d033</cites><orcidid>0000-0002-5181-1255</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12550-023-00509-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12550-023-00509-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37968430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Jong-Cheol</creatorcontrib><creatorcontrib>Hwang, In Min</creatorcontrib><creatorcontrib>Kim, Ho Myeong</creatorcontrib><creatorcontrib>Kim, Seulbi</creatorcontrib><creatorcontrib>Shin, Teak Su</creatorcontrib><creatorcontrib>Woo, Soo-Dong</creatorcontrib><creatorcontrib>Park, Hae Woong</creatorcontrib><title>Rapid analysis of insecticidal metabolites from the entomopathogenic fungus Beauveria bassiana 331R using UPLC-Q-Orbitrap MS</title><title>Mycotoxin research</title><addtitle>Mycotoxin Res</addtitle><addtitle>Mycotoxin Res</addtitle><description>Beauveria bassiana
, a representative entomopathogenic fungus, is increasingly being utilized as an eco-friendly pest management alternative to chemical insecticides. This fungus produces a range of insecticidal secondary metabolites that act as antimicrobial and immunosuppressive agents. However, detailed qualitative and quantitative analysis related to these compounds remains scarce, we developed a method for the rapid analysis of these metabolites. Eight secondary metabolites (bassianin, bassianolide, beauvericin, beauveriolide I, enniatin A, A1, and B, and tenellin) were efficiently extracted when
B. bassiana
-infected
Tenebrio molitor
larvae were ground in 70% EtOH extraction solvent and subsequently subjected to ultrasonic treatment for 30 min. The eight metabolites were rapidly and simultaneously analyzed using ultra-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). Bassianolide (20.6–51.1 µg/g) and beauvericin (63.6–109.8 µg/g) were identified as the main metabolites in
B. basssiana
-infected larvae, indicating that they are likely major toxins of
B. bassiana
. Validation of the method exhibited recovery rates in the range of 80–115% and precision in the range of 0.1–8.0%, indicating no significant interference from compounds in the matrix. We developed a method to rapidly analyze eight insecticidal metabolites using UPLC-Q-Orbitrap MS. This can be extensively utilized for detecting and producing insecticidal fungal secondary metabolites.</description><subject>Animals</subject><subject>Beauveria - metabolism</subject><subject>Beauveria bassiana</subject><subject>Beauvericin</subject><subject>Biomedical and Life Sciences</subject><subject>Chemistry/Food Science</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Entomopathogenic fungi</subject><subject>Fungi</subject><subject>immunosuppression</subject><subject>Immunosuppressive agents</subject><subject>Insecticides</subject><subject>Larva</subject><subject>Larvae</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical Microbiology</subject><subject>Medicine/Public Health</subject><subject>Metabolites</subject><subject>Microbiology</subject><subject>mycotoxins</subject><subject>Original Article</subject><subject>Pest control</subject><subject>pest management</subject><subject>Quadrupoles</subject><subject>Qualitative analysis</subject><subject>quantitative analysis</subject><subject>rapid methods</subject><subject>Secondary metabolites</subject><subject>solvents</subject><subject>Tenebrio</subject><subject>Tenebrio molitor</subject><subject>Toxins</subject><subject>Ultrasonic imaging</subject><subject>Ultrasonic processing</subject><subject>ultrasonic treatment</subject><issn>0178-7888</issn><issn>1867-1632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EotvCC3BAlrhwMYztOLaPsCoUaVGh0HPkOJOtqyQOdoK0Eg-PYQtIHOA0h_nmH818hDzh8IID6JeZC6WAgZAMQIFlh3tkw02tGa-luE82wLVh2hhzQk5zvgWoZVWbh-REalubSsKGfLtyc-iom9xwyCHT2NMwZfRL8KFzAx1xcW0cwoKZ9imOdLlBitMSxzi75SbucQqe9uu0XzN9jW79iik42rqcQwmlUvIruuYw7en1h92WfWSXqQ1LcjN9_-kRedC7IePju3pGrt-cf95esN3l23fbVzvmpbILMyh6AzWvKi-0bG2lsO77uvXgO20dlqts1RrQthPKCK6wQsSq9VXvdQdSnpHnx9w5xS8r5qUZQ_Y4DG7CuOZGciVrMKr853-oMBa0Usrqgj77C72NayqPLJQVnCtjlCqUOFI-xZwT9s2cwujSoeHQ_NDYHDU2RWPzU2NzKENP76LXdsTu98gvbwWQRyCX1rTH9Gf3P2K_A3y4qG4</recordid><startdate>20240201</startdate><enddate>20240201</enddate><creator>Kim, Jong-Cheol</creator><creator>Hwang, In Min</creator><creator>Kim, Ho Myeong</creator><creator>Kim, Seulbi</creator><creator>Shin, Teak Su</creator><creator>Woo, Soo-Dong</creator><creator>Park, Hae Woong</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-5181-1255</orcidid></search><sort><creationdate>20240201</creationdate><title>Rapid analysis of insecticidal metabolites from the entomopathogenic fungus Beauveria bassiana 331R using UPLC-Q-Orbitrap MS</title><author>Kim, Jong-Cheol ; Hwang, In Min ; Kim, Ho Myeong ; Kim, Seulbi ; Shin, Teak Su ; Woo, Soo-Dong ; Park, Hae Woong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-8e2f806144c273b945e6ff6bc0cd79ae63494b8079d258215e4eee4bc4fc7d033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Beauveria - metabolism</topic><topic>Beauveria bassiana</topic><topic>Beauvericin</topic><topic>Biomedical and Life Sciences</topic><topic>Chemistry/Food Science</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Entomopathogenic fungi</topic><topic>Fungi</topic><topic>immunosuppression</topic><topic>Immunosuppressive agents</topic><topic>Insecticides</topic><topic>Larva</topic><topic>Larvae</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical Microbiology</topic><topic>Medicine/Public Health</topic><topic>Metabolites</topic><topic>Microbiology</topic><topic>mycotoxins</topic><topic>Original Article</topic><topic>Pest control</topic><topic>pest management</topic><topic>Quadrupoles</topic><topic>Qualitative analysis</topic><topic>quantitative analysis</topic><topic>rapid methods</topic><topic>Secondary metabolites</topic><topic>solvents</topic><topic>Tenebrio</topic><topic>Tenebrio molitor</topic><topic>Toxins</topic><topic>Ultrasonic imaging</topic><topic>Ultrasonic processing</topic><topic>ultrasonic treatment</topic><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jong-Cheol</creatorcontrib><creatorcontrib>Hwang, In Min</creatorcontrib><creatorcontrib>Kim, Ho Myeong</creatorcontrib><creatorcontrib>Kim, Seulbi</creatorcontrib><creatorcontrib>Shin, Teak Su</creatorcontrib><creatorcontrib>Woo, Soo-Dong</creatorcontrib><creatorcontrib>Park, Hae Woong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Mycotoxin research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jong-Cheol</au><au>Hwang, In Min</au><au>Kim, Ho Myeong</au><au>Kim, Seulbi</au><au>Shin, Teak Su</au><au>Woo, Soo-Dong</au><au>Park, Hae Woong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid analysis of insecticidal metabolites from the entomopathogenic fungus Beauveria bassiana 331R using UPLC-Q-Orbitrap MS</atitle><jtitle>Mycotoxin research</jtitle><stitle>Mycotoxin Res</stitle><addtitle>Mycotoxin Res</addtitle><date>2024-02-01</date><risdate>2024</risdate><volume>40</volume><issue>1</issue><spage>123</spage><epage>132</epage><pages>123-132</pages><issn>0178-7888</issn><eissn>1867-1632</eissn><abstract>Beauveria bassiana
, a representative entomopathogenic fungus, is increasingly being utilized as an eco-friendly pest management alternative to chemical insecticides. This fungus produces a range of insecticidal secondary metabolites that act as antimicrobial and immunosuppressive agents. However, detailed qualitative and quantitative analysis related to these compounds remains scarce, we developed a method for the rapid analysis of these metabolites. Eight secondary metabolites (bassianin, bassianolide, beauvericin, beauveriolide I, enniatin A, A1, and B, and tenellin) were efficiently extracted when
B. bassiana
-infected
Tenebrio molitor
larvae were ground in 70% EtOH extraction solvent and subsequently subjected to ultrasonic treatment for 30 min. The eight metabolites were rapidly and simultaneously analyzed using ultra-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). Bassianolide (20.6–51.1 µg/g) and beauvericin (63.6–109.8 µg/g) were identified as the main metabolites in
B. basssiana
-infected larvae, indicating that they are likely major toxins of
B. bassiana
. Validation of the method exhibited recovery rates in the range of 80–115% and precision in the range of 0.1–8.0%, indicating no significant interference from compounds in the matrix. We developed a method to rapidly analyze eight insecticidal metabolites using UPLC-Q-Orbitrap MS. This can be extensively utilized for detecting and producing insecticidal fungal secondary metabolites.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37968430</pmid><doi>10.1007/s12550-023-00509-y</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-5181-1255</orcidid></addata></record> |
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| subjects | Animals Beauveria - metabolism Beauveria bassiana Beauvericin Biomedical and Life Sciences Chemistry/Food Science Chromatography, High Pressure Liquid Entomopathogenic fungi Fungi immunosuppression Immunosuppressive agents Insecticides Larva Larvae Life Sciences Liquid chromatography Mass spectrometry Mass spectroscopy Medical Microbiology Medicine/Public Health Metabolites Microbiology mycotoxins Original Article Pest control pest management Quadrupoles Qualitative analysis quantitative analysis rapid methods Secondary metabolites solvents Tenebrio Tenebrio molitor Toxins Ultrasonic imaging Ultrasonic processing ultrasonic treatment |
| title | Rapid analysis of insecticidal metabolites from the entomopathogenic fungus Beauveria bassiana 331R using UPLC-Q-Orbitrap MS |
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