Precursor-Directed Biosynthesis of Neoantimycin Derivatives with Selective Cytotoxicity

A precursor-directed biosynthesis approach led to the accumulation of seven new neoantimycin derivatives (1–7) from Streptomyces conglobatus RJ2. Structure elucidation was conducted using NMR and HRESIMS analysis, and the absolute configuration was determined by advanced Marfey’s method, Mosher’s an...

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Veröffentlicht in:	Journal of natural products (Washington, D.C.) D.C.), 2024-05, Vol.87 (5), p.1376-1383
Hauptverfasser:	Li, Zhengyuan, Chai, Ling, Tang, Zhenzhou, Zhu, Hongrui, Xue, Peiying, Sun, Fan, Lin, Houwen, Zhou, Yongjun, Lin, Xiao
Format:	Artikel
Sprache:	eng
Schlagworte:	Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology biosynthesis colorectal neoplasms cytotoxicity Drug Screening Assays, Antitumor Humans Molecular Structure Mutation Nuclear Magnetic Resonance, Biomolecular Organic Chemicals Streptomyces Streptomyces - chemistry Streptomyces - metabolism
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container_end_page	1383
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container_title	Journal of natural products (Washington, D.C.)
container_volume	87
creator	Li, Zhengyuan Chai, Ling Tang, Zhenzhou Zhu, Hongrui Xue, Peiying Sun, Fan Lin, Houwen Zhou, Yongjun Lin, Xiao
description	A precursor-directed biosynthesis approach led to the accumulation of seven new neoantimycin derivatives (1–7) from Streptomyces conglobatus RJ2. Structure elucidation was conducted using NMR and HRESIMS analysis, and the absolute configuration was determined by advanced Marfey’s method, Mosher’s analysis, and ECD analysis. The obtained compounds revealed selective and significant cytotoxicity, specifically against colorectal cancer cells bearing the K-ras mutation, with IC50 values ranging from 40 nM to 3.5 μM.
doi_str_mv	10.1021/acs.jnatprod.4c00013
format	Article
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source	MEDLINE; ACS Publications
subjects	Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology biosynthesis colorectal neoplasms cytotoxicity Drug Screening Assays, Antitumor Humans Molecular Structure Mutation Nuclear Magnetic Resonance, Biomolecular Organic Chemicals Streptomyces Streptomyces - chemistry Streptomyces - metabolism
title	Precursor-Directed Biosynthesis of Neoantimycin Derivatives with Selective Cytotoxicity
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