Sex-Biased Transcription Expression of Vitellogenins Reveals Fusion Gene and MicroRNA Regulation in the Sea Louse Caligus rogercresseyi
The caligid ectoparasite, Caligus rogercresseyi , is one of the main concerns in the Chilean salmon industry. The molecular mechanisms displayed by the parasite during the reproductive process represent an opportunity for developing novel control strategies. Vitellogenin is a multifunctional protein...
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| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2024-04, Vol.26 (2), p.243-260 |
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| creator | Casuso, Antonio Benavente, Bárbara P. Leal, Yeny Carrera-Naipil, Crisleri Valenzuela-Muñoz, Valentina Gallardo-Escárate, Cristian |
| description | The caligid ectoparasite,
Caligus rogercresseyi
, is one of the main concerns in the Chilean salmon industry. The molecular mechanisms displayed by the parasite during the reproductive process represent an opportunity for developing novel control strategies. Vitellogenin is a multifunctional protein recognized as a critical player in several crustaceans’ biological processes, including reproduction, embryonic development, and immune response. This study aimed to characterize the
C. rogercresseyi
vitellogenins, including discovering novel transcripts and regulatory mechanisms associated with microRNAs. Herein, vitellogenin genes were identified by homology analysis using the reference sea louse genome, transcriptome database, and arthropods vitellogenin-protein database. The validation of expression transcripts was conducted by RNA nanopore sequencing technology. Moreover, fusion gene profiling, miRNA target analysis, and functional validation were performed using luciferase assay. Six putative vitellogenin genes were identified in the
C. rogercresseyi
genome with high homology with other copepods vitellogenins. Furthermore, miR-996 showed a putative role in regulating the
Cr_Vitellogenin1
gene, which is highly expressed in females. Moreover, vitellogenin-fusion genes were identified in adult stages and highly regulated in males, demonstrating sex-related expression patterns. In females, the identified fusion genes merged with several non-vitellogenin genes involved in biological processes of ribosome assembly, BMP signaling pathway, and biosynthetic processes. This study reports the genome array of vitellogenins in
C. rogercresseyi
for the first time, revealing the putative role of fusion genes and miRNA regulation in sea lice biology. |
| doi_str_mv | 10.1007/s10126-024-10291-2 |
| format | Article |
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Caligus rogercresseyi
, is one of the main concerns in the Chilean salmon industry. The molecular mechanisms displayed by the parasite during the reproductive process represent an opportunity for developing novel control strategies. Vitellogenin is a multifunctional protein recognized as a critical player in several crustaceans’ biological processes, including reproduction, embryonic development, and immune response. This study aimed to characterize the
C. rogercresseyi
vitellogenins, including discovering novel transcripts and regulatory mechanisms associated with microRNAs. Herein, vitellogenin genes were identified by homology analysis using the reference sea louse genome, transcriptome database, and arthropods vitellogenin-protein database. The validation of expression transcripts was conducted by RNA nanopore sequencing technology. Moreover, fusion gene profiling, miRNA target analysis, and functional validation were performed using luciferase assay. Six putative vitellogenin genes were identified in the
C. rogercresseyi
genome with high homology with other copepods vitellogenins. Furthermore, miR-996 showed a putative role in regulating the
Cr_Vitellogenin1
gene, which is highly expressed in females. Moreover, vitellogenin-fusion genes were identified in adult stages and highly regulated in males, demonstrating sex-related expression patterns. In females, the identified fusion genes merged with several non-vitellogenin genes involved in biological processes of ribosome assembly, BMP signaling pathway, and biosynthetic processes. This study reports the genome array of vitellogenins in
C. rogercresseyi
for the first time, revealing the putative role of fusion genes and miRNA regulation in sea lice biology.</description><identifier>ISSN: 1436-2228</identifier><identifier>ISSN: 1436-2236</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-024-10291-2</identifier><identifier>PMID: 38294574</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>adults ; Aquatic crustaceans ; Arthropods ; Biological activity ; Biology ; Biomedical and Life Sciences ; biosynthesis ; Caligus rogercresseyi ; Crustaceans ; Defence mechanisms ; Ectoparasites ; Embryogenesis ; Embryonic development ; Embryonic growth stage ; Engineering ; Females ; fish industry ; Freshwater & Marine Ecology ; Fusion protein ; Gene expression ; Gene fusion ; Gene regulation ; Gene sequencing ; Genes ; Genomes ; Homology ; Immune response ; Lice ; Life Sciences ; luciferase ; Microbiology ; microRNA ; MicroRNAs ; miRNA ; Molecular modelling ; nanopores ; Proteins ; Regulatory mechanisms (biology) ; reproduction ; Ribonucleic acid ; Ribosomes ; RNA ; Salmon ; Sex ; Signal transduction ; Transcription ; transcriptome ; Transcriptomes ; Vitellogenin ; Zoology</subject><ispartof>Marine biotechnology (New York, N.Y.), 2024-04, Vol.26 (2), p.243-260</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024</rights><rights>2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-8c63ca3fd5503a0cbd30f5c74a18493b8bb14f8b6f01be5241d2878fe3edbf683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10126-024-10291-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10126-024-10291-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38294574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Casuso, Antonio</creatorcontrib><creatorcontrib>Benavente, Bárbara P.</creatorcontrib><creatorcontrib>Leal, Yeny</creatorcontrib><creatorcontrib>Carrera-Naipil, Crisleri</creatorcontrib><creatorcontrib>Valenzuela-Muñoz, Valentina</creatorcontrib><creatorcontrib>Gallardo-Escárate, Cristian</creatorcontrib><title>Sex-Biased Transcription Expression of Vitellogenins Reveals Fusion Gene and MicroRNA Regulation in the Sea Louse Caligus rogercresseyi</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>The caligid ectoparasite,
Caligus rogercresseyi
, is one of the main concerns in the Chilean salmon industry. The molecular mechanisms displayed by the parasite during the reproductive process represent an opportunity for developing novel control strategies. Vitellogenin is a multifunctional protein recognized as a critical player in several crustaceans’ biological processes, including reproduction, embryonic development, and immune response. This study aimed to characterize the
C. rogercresseyi
vitellogenins, including discovering novel transcripts and regulatory mechanisms associated with microRNAs. Herein, vitellogenin genes were identified by homology analysis using the reference sea louse genome, transcriptome database, and arthropods vitellogenin-protein database. The validation of expression transcripts was conducted by RNA nanopore sequencing technology. Moreover, fusion gene profiling, miRNA target analysis, and functional validation were performed using luciferase assay. Six putative vitellogenin genes were identified in the
C. rogercresseyi
genome with high homology with other copepods vitellogenins. Furthermore, miR-996 showed a putative role in regulating the
Cr_Vitellogenin1
gene, which is highly expressed in females. Moreover, vitellogenin-fusion genes were identified in adult stages and highly regulated in males, demonstrating sex-related expression patterns. In females, the identified fusion genes merged with several non-vitellogenin genes involved in biological processes of ribosome assembly, BMP signaling pathway, and biosynthetic processes. This study reports the genome array of vitellogenins in
C. rogercresseyi
for the first time, revealing the putative role of fusion genes and miRNA regulation in sea lice biology.</description><subject>adults</subject><subject>Aquatic crustaceans</subject><subject>Arthropods</subject><subject>Biological activity</subject><subject>Biology</subject><subject>Biomedical and Life Sciences</subject><subject>biosynthesis</subject><subject>Caligus rogercresseyi</subject><subject>Crustaceans</subject><subject>Defence mechanisms</subject><subject>Ectoparasites</subject><subject>Embryogenesis</subject><subject>Embryonic development</subject><subject>Embryonic growth stage</subject><subject>Engineering</subject><subject>Females</subject><subject>fish industry</subject><subject>Freshwater & Marine Ecology</subject><subject>Fusion protein</subject><subject>Gene expression</subject><subject>Gene fusion</subject><subject>Gene regulation</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Homology</subject><subject>Immune response</subject><subject>Lice</subject><subject>Life Sciences</subject><subject>luciferase</subject><subject>Microbiology</subject><subject>microRNA</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Molecular modelling</subject><subject>nanopores</subject><subject>Proteins</subject><subject>Regulatory mechanisms (biology)</subject><subject>reproduction</subject><subject>Ribonucleic acid</subject><subject>Ribosomes</subject><subject>RNA</subject><subject>Salmon</subject><subject>Sex</subject><subject>Signal transduction</subject><subject>Transcription</subject><subject>transcriptome</subject><subject>Transcriptomes</subject><subject>Vitellogenin</subject><subject>Zoology</subject><issn>1436-2228</issn><issn>1436-2236</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u1DAUhS1ERUvhBVggS2y6Cfg_zrKM-icNrdQWtpbjXA-uMs5gJ6h9Al4bZ6YUiQVd-Ur-7jn33oPQO0o-UkLqT5kSylRFmKgoYQ2t2At0QAVXFWNcvXyqmd5Hr3O-I6Wp5uQV2ueaNULW4gD9uoH76nOwGTp8m2zMLoXNGIaIT-43CXKey8Hjb2GEvh9WEEPM-Bp-gu0zPp22_2cQAdvY4S_BpeH68rgAq6m3W50Q8fgd8A1YvBymDHhh-7CaMk5FLbnZAx7CG7TniyK8fXwP0dfTk9vFebW8OrtYHC8rx2UzVtop7iz3nZSEW-LajhMvXS0s1aLhrW5bKrxulSe0BckE7ZiutQcOXeuV5ofoaKe7ScOPCfJo1iG7spqNUKYznMpipIliz6KsYUTWVGlV0A__oHfDlGJZxHAiJC9qZPZmO6ocKecE3mxSWNv0YCgxc6Jml6gpiZptomae4v2j9NSuoXtq-RNhAfgOyOUrlpP-9f6P7G84Yax6</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Casuso, Antonio</creator><creator>Benavente, Bárbara P.</creator><creator>Leal, Yeny</creator><creator>Carrera-Naipil, Crisleri</creator><creator>Valenzuela-Muñoz, Valentina</creator><creator>Gallardo-Escárate, Cristian</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240401</creationdate><title>Sex-Biased Transcription Expression of Vitellogenins Reveals Fusion Gene and MicroRNA Regulation in the Sea Louse Caligus rogercresseyi</title><author>Casuso, Antonio ; 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Caligus rogercresseyi
, is one of the main concerns in the Chilean salmon industry. The molecular mechanisms displayed by the parasite during the reproductive process represent an opportunity for developing novel control strategies. Vitellogenin is a multifunctional protein recognized as a critical player in several crustaceans’ biological processes, including reproduction, embryonic development, and immune response. This study aimed to characterize the
C. rogercresseyi
vitellogenins, including discovering novel transcripts and regulatory mechanisms associated with microRNAs. Herein, vitellogenin genes were identified by homology analysis using the reference sea louse genome, transcriptome database, and arthropods vitellogenin-protein database. The validation of expression transcripts was conducted by RNA nanopore sequencing technology. Moreover, fusion gene profiling, miRNA target analysis, and functional validation were performed using luciferase assay. Six putative vitellogenin genes were identified in the
C. rogercresseyi
genome with high homology with other copepods vitellogenins. Furthermore, miR-996 showed a putative role in regulating the
Cr_Vitellogenin1
gene, which is highly expressed in females. Moreover, vitellogenin-fusion genes were identified in adult stages and highly regulated in males, demonstrating sex-related expression patterns. In females, the identified fusion genes merged with several non-vitellogenin genes involved in biological processes of ribosome assembly, BMP signaling pathway, and biosynthetic processes. This study reports the genome array of vitellogenins in
C. rogercresseyi
for the first time, revealing the putative role of fusion genes and miRNA regulation in sea lice biology.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38294574</pmid><doi>10.1007/s10126-024-10291-2</doi><tpages>18</tpages></addata></record> |
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| source | SpringerLink Journals |
| subjects | adults Aquatic crustaceans Arthropods Biological activity Biology Biomedical and Life Sciences biosynthesis Caligus rogercresseyi Crustaceans Defence mechanisms Ectoparasites Embryogenesis Embryonic development Embryonic growth stage Engineering Females fish industry Freshwater & Marine Ecology Fusion protein Gene expression Gene fusion Gene regulation Gene sequencing Genes Genomes Homology Immune response Lice Life Sciences luciferase Microbiology microRNA MicroRNAs miRNA Molecular modelling nanopores Proteins Regulatory mechanisms (biology) reproduction Ribonucleic acid Ribosomes RNA Salmon Sex Signal transduction Transcription transcriptome Transcriptomes Vitellogenin Zoology |
| title | Sex-Biased Transcription Expression of Vitellogenins Reveals Fusion Gene and MicroRNA Regulation in the Sea Louse Caligus rogercresseyi |
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