Study of the effect of calcium signal participating in the antioxidant mechanism of yeast under high‐sugar environment

BACKGROUND Saccharomyces cerevisiae is susceptible to high‐sugar stress in the production of bioethanol, wine and bread. Calcium signal is widely involved in various physiological and metabolic activities of cells. The present study aimed to explore the effects of Ca2+ signal on the antioxidant mech...

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Veröffentlicht in:Journal of the science of food and agriculture 2024-08, Vol.104 (10), p.5776-5788
Hauptverfasser: Xie, Dongdong, Sun, Yingqi, Li, Xing, Zheng, Jiaxin, Ren, Shuncheng
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container_end_page 5788
container_issue 10
container_start_page 5776
container_title Journal of the science of food and agriculture
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creator Xie, Dongdong
Sun, Yingqi
Li, Xing
Zheng, Jiaxin
Ren, Shuncheng
description BACKGROUND Saccharomyces cerevisiae is susceptible to high‐sugar stress in the production of bioethanol, wine and bread. Calcium signal is widely involved in various physiological and metabolic activities of cells. The present study aimed to explore the effects of Ca2+ signal on the antioxidant mechanism of yeast during high‐sugar fermentation. RESULTS Compared to yeast without available Ca2+, yeast in the high glucose with Ca2+ group had higher dry weight, higher ethanol output at 12 and 24 h and higher glycerol output at 24 and 36 h. During the whole growth process, the trehalose synthesis capacity of yeast in the high glucose with Ca2+ group was lower and intracellular reactive oxygen species content was higher compared to yeast without available Ca2+. Intracellular malondialdehyde content of yeast under high glucose with Ca2+ was significantly lower than yeast under high glucose without available Ca2+ except for 6 h. The superoxide dismutase and catalase activities of yeast and glutathione content were higher in the high glucose with Ca2+ group compared to yeast in high glucose without available Ca2+. The expression levels of SOD1, GSH1, GPX2 genes were higher for high glucose without available Ca2+ at 6 h, while yeast in the high glucose with Ca2+ group had a higher expression of antioxidant‐related genes except SOD1 and CTT1 at 12 h. The expression levels of antioxidant‐related genes of yeast for high glucose with Ca2+ were higher at 24 h, and those of genes except SOD1 of yeast in the high glucose with Ca2+ group were higher at 36 h. CONCLUSION High‐glucose stress limited the growth of yeast, while a moderate extracellular Ca2+ signal could improve the antioxidant capacity of yeast in a high‐glucose environment by regulating protectant metabolism and enhancing the antioxidant enzyme activity and expression of antioxidant genes in a high‐sugar environment. © 2024 Society of Chemical Industry.
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Calcium signal is widely involved in various physiological and metabolic activities of cells. The present study aimed to explore the effects of Ca2+ signal on the antioxidant mechanism of yeast during high‐sugar fermentation. RESULTS Compared to yeast without available Ca2+, yeast in the high glucose with Ca2+ group had higher dry weight, higher ethanol output at 12 and 24 h and higher glycerol output at 24 and 36 h. During the whole growth process, the trehalose synthesis capacity of yeast in the high glucose with Ca2+ group was lower and intracellular reactive oxygen species content was higher compared to yeast without available Ca2+. Intracellular malondialdehyde content of yeast under high glucose with Ca2+ was significantly lower than yeast under high glucose without available Ca2+ except for 6 h. The superoxide dismutase and catalase activities of yeast and glutathione content were higher in the high glucose with Ca2+ group compared to yeast in high glucose without available Ca2+. The expression levels of SOD1, GSH1, GPX2 genes were higher for high glucose without available Ca2+ at 6 h, while yeast in the high glucose with Ca2+ group had a higher expression of antioxidant‐related genes except SOD1 and CTT1 at 12 h. The expression levels of antioxidant‐related genes of yeast for high glucose with Ca2+ were higher at 24 h, and those of genes except SOD1 of yeast in the high glucose with Ca2+ group were higher at 36 h. CONCLUSION High‐glucose stress limited the growth of yeast, while a moderate extracellular Ca2+ signal could improve the antioxidant capacity of yeast in a high‐glucose environment by regulating protectant metabolism and enhancing the antioxidant enzyme activity and expression of antioxidant genes in a high‐sugar environment. © 2024 Society of Chemical Industry.</description><identifier>ISSN: 0022-5142</identifier><identifier>ISSN: 1097-0010</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.13411</identifier><identifier>PMID: 38390983</identifier><language>eng</language><publisher>Chichester, UK: John Wiley &amp; Sons, Ltd</publisher><subject>agriculture ; antioxidant activity ; antioxidant enzymes ; Antioxidants ; bioethanol ; Biofuels ; breads ; Calcium ; Calcium (extracellular) ; Calcium (intracellular) ; Calcium ions ; calcium signal ; calcium signaling ; Calcium signalling ; Catalase ; Enzymatic activity ; Enzyme activity ; Ethanol ; Fermentation ; Gene expression ; Genes ; Glucose ; Glutathione ; glycerol ; high glucose ; Intracellular ; malondialdehyde ; Reactive oxygen species ; Saccharomyces cerevisiae ; Sugar ; Superoxide dismutase ; Trehalose ; Wines ; Yeast ; Yeasts</subject><ispartof>Journal of the science of food and agriculture, 2024-08, Vol.104 (10), p.5776-5788</ispartof><rights>2024 Society of Chemical Industry.</rights><rights>This article is protected by copyright. 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Calcium signal is widely involved in various physiological and metabolic activities of cells. The present study aimed to explore the effects of Ca2+ signal on the antioxidant mechanism of yeast during high‐sugar fermentation. RESULTS Compared to yeast without available Ca2+, yeast in the high glucose with Ca2+ group had higher dry weight, higher ethanol output at 12 and 24 h and higher glycerol output at 24 and 36 h. During the whole growth process, the trehalose synthesis capacity of yeast in the high glucose with Ca2+ group was lower and intracellular reactive oxygen species content was higher compared to yeast without available Ca2+. Intracellular malondialdehyde content of yeast under high glucose with Ca2+ was significantly lower than yeast under high glucose without available Ca2+ except for 6 h. The superoxide dismutase and catalase activities of yeast and glutathione content were higher in the high glucose with Ca2+ group compared to yeast in high glucose without available Ca2+. The expression levels of SOD1, GSH1, GPX2 genes were higher for high glucose without available Ca2+ at 6 h, while yeast in the high glucose with Ca2+ group had a higher expression of antioxidant‐related genes except SOD1 and CTT1 at 12 h. The expression levels of antioxidant‐related genes of yeast for high glucose with Ca2+ were higher at 24 h, and those of genes except SOD1 of yeast in the high glucose with Ca2+ group were higher at 36 h. CONCLUSION High‐glucose stress limited the growth of yeast, while a moderate extracellular Ca2+ signal could improve the antioxidant capacity of yeast in a high‐glucose environment by regulating protectant metabolism and enhancing the antioxidant enzyme activity and expression of antioxidant genes in a high‐sugar environment. © 2024 Society of Chemical Industry.</description><subject>agriculture</subject><subject>antioxidant activity</subject><subject>antioxidant enzymes</subject><subject>Antioxidants</subject><subject>bioethanol</subject><subject>Biofuels</subject><subject>breads</subject><subject>Calcium</subject><subject>Calcium (extracellular)</subject><subject>Calcium (intracellular)</subject><subject>Calcium ions</subject><subject>calcium signal</subject><subject>calcium signaling</subject><subject>Calcium signalling</subject><subject>Catalase</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Ethanol</subject><subject>Fermentation</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Glucose</subject><subject>Glutathione</subject><subject>glycerol</subject><subject>high glucose</subject><subject>Intracellular</subject><subject>malondialdehyde</subject><subject>Reactive oxygen species</subject><subject>Saccharomyces cerevisiae</subject><subject>Sugar</subject><subject>Superoxide dismutase</subject><subject>Trehalose</subject><subject>Wines</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0022-5142</issn><issn>1097-0010</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc1qGzEQx0VIady0lzxAEeRSCk5GH7srHUNo-kGgh6TnRdaObJldrSNp0_jWR-gz9kkqx2kPPaQIZhDz0w9Gf0JOGJwxAH6-Ts6cMSEZOyAzBrqZAzA4JLMy5POKSX5EXqW0BgCt6_olORJKaNBKzMjDTZ66LR0dzSuk6BzavLtZ01s_DTT5ZTA93ZiYvfUbk31YUh8eaROyHx98Vzod0K5M8GnYPd6iSZlOocNIV365-vXjZ5qWJlIM9z6OYcCQX5MXzvQJ3zz1Y_Lt6sPt5af59dePny8vrudWSM1KrcSiUaC6jgusGyVBYmNsx7hqtObaNQKgs0yJBTPMOFlD2cIhNIJra8Qxebf3buJ4N2HK7eCTxb43AccptYJVotJS6eq_KNdC8XKULOjpP-h6nGL5qSKEWmkttGSFer-nbBxTiujaTfSDiduWQbuLrt1F1z5GV-C3T8ppMWD3F_2TVQHYHvjue9w-o2q_3Fxd7KW_AajHpOs</recordid><startdate>20240815</startdate><enddate>20240815</enddate><creator>Xie, Dongdong</creator><creator>Sun, Yingqi</creator><creator>Li, Xing</creator><creator>Zheng, Jiaxin</creator><creator>Ren, Shuncheng</creator><general>John Wiley &amp; 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Calcium signal is widely involved in various physiological and metabolic activities of cells. The present study aimed to explore the effects of Ca2+ signal on the antioxidant mechanism of yeast during high‐sugar fermentation. RESULTS Compared to yeast without available Ca2+, yeast in the high glucose with Ca2+ group had higher dry weight, higher ethanol output at 12 and 24 h and higher glycerol output at 24 and 36 h. During the whole growth process, the trehalose synthesis capacity of yeast in the high glucose with Ca2+ group was lower and intracellular reactive oxygen species content was higher compared to yeast without available Ca2+. Intracellular malondialdehyde content of yeast under high glucose with Ca2+ was significantly lower than yeast under high glucose without available Ca2+ except for 6 h. The superoxide dismutase and catalase activities of yeast and glutathione content were higher in the high glucose with Ca2+ group compared to yeast in high glucose without available Ca2+. The expression levels of SOD1, GSH1, GPX2 genes were higher for high glucose without available Ca2+ at 6 h, while yeast in the high glucose with Ca2+ group had a higher expression of antioxidant‐related genes except SOD1 and CTT1 at 12 h. The expression levels of antioxidant‐related genes of yeast for high glucose with Ca2+ were higher at 24 h, and those of genes except SOD1 of yeast in the high glucose with Ca2+ group were higher at 36 h. CONCLUSION High‐glucose stress limited the growth of yeast, while a moderate extracellular Ca2+ signal could improve the antioxidant capacity of yeast in a high‐glucose environment by regulating protectant metabolism and enhancing the antioxidant enzyme activity and expression of antioxidant genes in a high‐sugar environment. © 2024 Society of Chemical Industry.</abstract><cop>Chichester, UK</cop><pub>John Wiley &amp; Sons, Ltd</pub><pmid>38390983</pmid><doi>10.1002/jsfa.13411</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-6376-5252</orcidid></addata></record>
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source Wiley Online Library Journals Frontfile Complete
subjects agriculture
antioxidant activity
antioxidant enzymes
Antioxidants
bioethanol
Biofuels
breads
Calcium
Calcium (extracellular)
Calcium (intracellular)
Calcium ions
calcium signal
calcium signaling
Calcium signalling
Catalase
Enzymatic activity
Enzyme activity
Ethanol
Fermentation
Gene expression
Genes
Glucose
Glutathione
glycerol
high glucose
Intracellular
malondialdehyde
Reactive oxygen species
Saccharomyces cerevisiae
Sugar
Superoxide dismutase
Trehalose
Wines
Yeast
Yeasts
title Study of the effect of calcium signal participating in the antioxidant mechanism of yeast under high‐sugar environment
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