Relationship between biofilm-forming microorganisms (BFM: Staphylococcus aureus and Pseudomonas aeruginosa) and DEFB1 gene variants on β-defensin levels in periprosthetic joint infection (PJI)
Background The purpose of this study was to investigate the relationship between biofilm-forming microorganisms (BFM) and DEFB1 gene variants on β-defensin levels in patients with periprosthetic joint infection (PJI) of Mexican origin. Methods and results One hundred and five clinical aspirates were...
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| Veröffentlicht in: | Molecular biology reports 2024-12, Vol.51 (1), p.237-237, Article 237 |
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| creator | Fernández-Torres, Javier Zamudio-Cuevas, Yessica Martínez-Flores, Karina Franco-Cendejas, Rafael |
| description | Background
The purpose of this study was to investigate the relationship between biofilm-forming microorganisms (BFM) and
DEFB1
gene variants on β-defensin levels in patients with periprosthetic joint infection (PJI) of Mexican origin.
Methods and results
One hundred and five clinical aspirates were obtained from patients with suspected PJI. After microbiologic culture, samples were classified as non-septic and septic; of the latter, only those positive for
Staphylococcus aureus
and
Pseudomonas aeruginosa
were selected. β-Defensin levels were quantified by ELISA, DNA was extracted from total leukocytes of the samples, and − 20G > A (rs11362) and − 44 C > G (rs1800972) variants were genotyped using TaqMan probes. Forty-one clinical aspirates were non-septic, 18 were positive for
S. aureus
and 18 were positive for
P. aeruginosa
. It was observed that β-defensin levels were higher in the
P. aeruginosa
group compared to
S. aureus
group (2339.0 pg/mL IQR = 1809.2 vs. 1821.3 pg/mL IQR = 1536.4) and non-septic group (2339.0 pg/mL IQR = 1809.2 vs. 1099.7 pg/mL IQR = 1744.5,
P
|
| doi_str_mv | 10.1007/s11033-023-09126-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153585780</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2920984120</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-52fcfd1fb0c9fad6af35769f3fa16cd0ee0e9e5d202ed0bd82c118c74ee1ddca3</originalsourceid><addsrcrecordid>eNqFks1u1DAQxyMEokvhBTggS1y2h4A_4k3CjZYuFBVR8XGOHHu861ViB0_Sqm_TZ-gjVJx5JrzdAhIHOFhje37z91jzz7KnjL5glJYvkTEqRE55WjXji1zey2ZMliIv6rK6n82ooCwvKsn2skeIG0ppwUr5MNsTFa8kL8Us-_4JOjW64HHtBtLCeAHgSeuCdV2f2xB751ekdzqGEFfKO-yRzA-XH17dXN1cfR7VsL7sgg5aT0jUFGEbvCFnCJMJffAqnSFOK-cDqoPb3Jvj5SEjK_BAzlV0yo9Igic_rnMDFjw6Tzo4hw5J2g0Q3RADjmsYnSab4PyY7i3obddkfvb-5OBx9sCqDuHJXdzPvi6Pvxy9y08_vj05en2aayHrMZfcamuYbamurTILZYUsF7UVVrGFNhSAQg3ScMrB0NZUXDNW6bIAYMZoJfaz-U43NfRtAhyb3qGGrlMewoSNYFLISpYV_S_Ka1aXhUwjSejzv9BNmKJPH0kUp3VVML6l-I5Kk0CMYJshul7Fy4bRZmuHZmeHJtmhubVDI1PRszvpqe3B_C75Nf8EiB2AKeVXEP-8_Q_Zn_lixqQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2920984120</pqid></control><display><type>article</type><title>Relationship between biofilm-forming microorganisms (BFM: Staphylococcus aureus and Pseudomonas aeruginosa) and DEFB1 gene variants on β-defensin levels in periprosthetic joint infection (PJI)</title><source>Springer Nature - Complete Springer Journals</source><creator>Fernández-Torres, Javier ; Zamudio-Cuevas, Yessica ; Martínez-Flores, Karina ; Franco-Cendejas, Rafael</creator><creatorcontrib>Fernández-Torres, Javier ; Zamudio-Cuevas, Yessica ; Martínez-Flores, Karina ; Franco-Cendejas, Rafael</creatorcontrib><description>Background
The purpose of this study was to investigate the relationship between biofilm-forming microorganisms (BFM) and
DEFB1
gene variants on β-defensin levels in patients with periprosthetic joint infection (PJI) of Mexican origin.
Methods and results
One hundred and five clinical aspirates were obtained from patients with suspected PJI. After microbiologic culture, samples were classified as non-septic and septic; of the latter, only those positive for
Staphylococcus aureus
and
Pseudomonas aeruginosa
were selected. β-Defensin levels were quantified by ELISA, DNA was extracted from total leukocytes of the samples, and − 20G > A (rs11362) and − 44 C > G (rs1800972) variants were genotyped using TaqMan probes. Forty-one clinical aspirates were non-septic, 18 were positive for
S. aureus
and 18 were positive for
P. aeruginosa
. It was observed that β-defensin levels were higher in the
P. aeruginosa
group compared to
S. aureus
group (2339.0 pg/mL IQR = 1809.2 vs. 1821.3 pg/mL IQR = 1536.4) and non-septic group (2339.0 pg/mL IQR = 1809.2 vs. 1099.7 pg/mL IQR = 1744.5,
P
< 0.001). The CG genotype of the rs1800972 variant was associated with higher β-defensin levels compared to the CC genotype for both
P. aeruginosa
and
S. aureus
(1905.8 vs. 421.7 pg/mL,
P
= 0.004; and 1878.2 vs. 256.4 pg/mL,
P
= 0.006, respectively).
Conclusions
Our results show that β-defensin levels are significantly elevated in patients with BFM-associated PJI compared to those without infection. Furthermore, carriers of the CG genotype of the rs1800972 variant have an increased risk of PJI. Further research is needed to replicate these findings in a larger population.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-023-09126-5</identifier><identifier>PMID: 38285273</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biofilms ; Biomedical and Life Sciences ; DNA ; DNA probes ; genes ; genotype ; Genotype & phenotype ; Genotypes ; genotyping ; Histology ; Joint diseases ; Leukocytes ; Life Sciences ; Microorganisms ; Morphology ; Original Article ; Pseudomonas aeruginosa ; risk ; Staphylococcus aureus ; Staphylococcus infections</subject><ispartof>Molecular biology reports, 2024-12, Vol.51 (1), p.237-237, Article 237</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-52fcfd1fb0c9fad6af35769f3fa16cd0ee0e9e5d202ed0bd82c118c74ee1ddca3</cites><orcidid>0000-0003-1574-1138 ; 0000-0002-7271-2862 ; 0000-0003-1751-3454 ; 0000-0003-0675-0227</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-023-09126-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-023-09126-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38285273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernández-Torres, Javier</creatorcontrib><creatorcontrib>Zamudio-Cuevas, Yessica</creatorcontrib><creatorcontrib>Martínez-Flores, Karina</creatorcontrib><creatorcontrib>Franco-Cendejas, Rafael</creatorcontrib><title>Relationship between biofilm-forming microorganisms (BFM: Staphylococcus aureus and Pseudomonas aeruginosa) and DEFB1 gene variants on β-defensin levels in periprosthetic joint infection (PJI)</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
The purpose of this study was to investigate the relationship between biofilm-forming microorganisms (BFM) and
DEFB1
gene variants on β-defensin levels in patients with periprosthetic joint infection (PJI) of Mexican origin.
Methods and results
One hundred and five clinical aspirates were obtained from patients with suspected PJI. After microbiologic culture, samples were classified as non-septic and septic; of the latter, only those positive for
Staphylococcus aureus
and
Pseudomonas aeruginosa
were selected. β-Defensin levels were quantified by ELISA, DNA was extracted from total leukocytes of the samples, and − 20G > A (rs11362) and − 44 C > G (rs1800972) variants were genotyped using TaqMan probes. Forty-one clinical aspirates were non-septic, 18 were positive for
S. aureus
and 18 were positive for
P. aeruginosa
. It was observed that β-defensin levels were higher in the
P. aeruginosa
group compared to
S. aureus
group (2339.0 pg/mL IQR = 1809.2 vs. 1821.3 pg/mL IQR = 1536.4) and non-septic group (2339.0 pg/mL IQR = 1809.2 vs. 1099.7 pg/mL IQR = 1744.5,
P
< 0.001). The CG genotype of the rs1800972 variant was associated with higher β-defensin levels compared to the CC genotype for both
P. aeruginosa
and
S. aureus
(1905.8 vs. 421.7 pg/mL,
P
= 0.004; and 1878.2 vs. 256.4 pg/mL,
P
= 0.006, respectively).
Conclusions
Our results show that β-defensin levels are significantly elevated in patients with BFM-associated PJI compared to those without infection. Furthermore, carriers of the CG genotype of the rs1800972 variant have an increased risk of PJI. Further research is needed to replicate these findings in a larger population.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biofilms</subject><subject>Biomedical and Life Sciences</subject><subject>DNA</subject><subject>DNA probes</subject><subject>genes</subject><subject>genotype</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>genotyping</subject><subject>Histology</subject><subject>Joint diseases</subject><subject>Leukocytes</subject><subject>Life Sciences</subject><subject>Microorganisms</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Pseudomonas aeruginosa</subject><subject>risk</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFks1u1DAQxyMEokvhBTggS1y2h4A_4k3CjZYuFBVR8XGOHHu861ViB0_Sqm_TZ-gjVJx5JrzdAhIHOFhje37z91jzz7KnjL5glJYvkTEqRE55WjXji1zey2ZMliIv6rK6n82ooCwvKsn2skeIG0ppwUr5MNsTFa8kL8Us-_4JOjW64HHtBtLCeAHgSeuCdV2f2xB751ekdzqGEFfKO-yRzA-XH17dXN1cfR7VsL7sgg5aT0jUFGEbvCFnCJMJffAqnSFOK-cDqoPb3Jvj5SEjK_BAzlV0yo9Igic_rnMDFjw6Tzo4hw5J2g0Q3RADjmsYnSab4PyY7i3obddkfvb-5OBx9sCqDuHJXdzPvi6Pvxy9y08_vj05en2aayHrMZfcamuYbamurTILZYUsF7UVVrGFNhSAQg3ScMrB0NZUXDNW6bIAYMZoJfaz-U43NfRtAhyb3qGGrlMewoSNYFLISpYV_S_Ka1aXhUwjSejzv9BNmKJPH0kUp3VVML6l-I5Kk0CMYJshul7Fy4bRZmuHZmeHJtmhubVDI1PRszvpqe3B_C75Nf8EiB2AKeVXEP-8_Q_Zn_lixqQ</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Fernández-Torres, Javier</creator><creator>Zamudio-Cuevas, Yessica</creator><creator>Martínez-Flores, Karina</creator><creator>Franco-Cendejas, Rafael</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-1574-1138</orcidid><orcidid>https://orcid.org/0000-0002-7271-2862</orcidid><orcidid>https://orcid.org/0000-0003-1751-3454</orcidid><orcidid>https://orcid.org/0000-0003-0675-0227</orcidid></search><sort><creationdate>20241201</creationdate><title>Relationship between biofilm-forming microorganisms (BFM: Staphylococcus aureus and Pseudomonas aeruginosa) and DEFB1 gene variants on β-defensin levels in periprosthetic joint infection (PJI)</title><author>Fernández-Torres, Javier ; Zamudio-Cuevas, Yessica ; Martínez-Flores, Karina ; Franco-Cendejas, Rafael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-52fcfd1fb0c9fad6af35769f3fa16cd0ee0e9e5d202ed0bd82c118c74ee1ddca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biofilms</topic><topic>Biomedical and Life Sciences</topic><topic>DNA</topic><topic>DNA probes</topic><topic>genes</topic><topic>genotype</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>genotyping</topic><topic>Histology</topic><topic>Joint diseases</topic><topic>Leukocytes</topic><topic>Life Sciences</topic><topic>Microorganisms</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Pseudomonas aeruginosa</topic><topic>risk</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus infections</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernández-Torres, Javier</creatorcontrib><creatorcontrib>Zamudio-Cuevas, Yessica</creatorcontrib><creatorcontrib>Martínez-Flores, Karina</creatorcontrib><creatorcontrib>Franco-Cendejas, Rafael</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernández-Torres, Javier</au><au>Zamudio-Cuevas, Yessica</au><au>Martínez-Flores, Karina</au><au>Franco-Cendejas, Rafael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship between biofilm-forming microorganisms (BFM: Staphylococcus aureus and Pseudomonas aeruginosa) and DEFB1 gene variants on β-defensin levels in periprosthetic joint infection (PJI)</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>51</volume><issue>1</issue><spage>237</spage><epage>237</epage><pages>237-237</pages><artnum>237</artnum><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
The purpose of this study was to investigate the relationship between biofilm-forming microorganisms (BFM) and
DEFB1
gene variants on β-defensin levels in patients with periprosthetic joint infection (PJI) of Mexican origin.
Methods and results
One hundred and five clinical aspirates were obtained from patients with suspected PJI. After microbiologic culture, samples were classified as non-septic and septic; of the latter, only those positive for
Staphylococcus aureus
and
Pseudomonas aeruginosa
were selected. β-Defensin levels were quantified by ELISA, DNA was extracted from total leukocytes of the samples, and − 20G > A (rs11362) and − 44 C > G (rs1800972) variants were genotyped using TaqMan probes. Forty-one clinical aspirates were non-septic, 18 were positive for
S. aureus
and 18 were positive for
P. aeruginosa
. It was observed that β-defensin levels were higher in the
P. aeruginosa
group compared to
S. aureus
group (2339.0 pg/mL IQR = 1809.2 vs. 1821.3 pg/mL IQR = 1536.4) and non-septic group (2339.0 pg/mL IQR = 1809.2 vs. 1099.7 pg/mL IQR = 1744.5,
P
< 0.001). The CG genotype of the rs1800972 variant was associated with higher β-defensin levels compared to the CC genotype for both
P. aeruginosa
and
S. aureus
(1905.8 vs. 421.7 pg/mL,
P
= 0.004; and 1878.2 vs. 256.4 pg/mL,
P
= 0.006, respectively).
Conclusions
Our results show that β-defensin levels are significantly elevated in patients with BFM-associated PJI compared to those without infection. Furthermore, carriers of the CG genotype of the rs1800972 variant have an increased risk of PJI. Further research is needed to replicate these findings in a larger population.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>38285273</pmid><doi>10.1007/s11033-023-09126-5</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1574-1138</orcidid><orcidid>https://orcid.org/0000-0002-7271-2862</orcidid><orcidid>https://orcid.org/0000-0003-1751-3454</orcidid><orcidid>https://orcid.org/0000-0003-0675-0227</orcidid></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Animal Anatomy Animal Biochemistry Biofilms Biomedical and Life Sciences DNA DNA probes genes genotype Genotype & phenotype Genotypes genotyping Histology Joint diseases Leukocytes Life Sciences Microorganisms Morphology Original Article Pseudomonas aeruginosa risk Staphylococcus aureus Staphylococcus infections |
| title | Relationship between biofilm-forming microorganisms (BFM: Staphylococcus aureus and Pseudomonas aeruginosa) and DEFB1 gene variants on β-defensin levels in periprosthetic joint infection (PJI) |
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