Molecular interaction mechanisms and cellular response of superoxide dismutase and catalase to fluoranthene
As an important raw material and intermediate product of the petrochemical industry, fluoranthene (Fla) can be emitted with industrial activities and has become a typical polycyclic aromatic hydrocarbon enriched in the Chinese topsoil layer, posing a significant threat to sensitive soil biota. Here,...
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| Veröffentlicht in: | Environmental science and pollution research international 2023-10, Vol.30 (47), p.104233-104245 |
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| creator | Song, Yan Sun, Kailun Zhao, Qiang Li, Yuze Liu, Guiqing Liu, Rutao |
| description | As an important raw material and intermediate product of the petrochemical industry, fluoranthene (Fla) can be emitted with industrial activities and has become a typical polycyclic aromatic hydrocarbon enriched in the Chinese topsoil layer, posing a significant threat to sensitive soil biota. Here, multispectral tools and molecular simulation techniques were integrated to elucidate the molecular mechanism of Fla interaction with key antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) at the molecular level. Meanwhile, we further revealed the cellular responses of SOD and CAT and the associated redox states in earthworm (
Eisenia fetida
) coelomocytes based on the molecular-level results. Our results showed that the exposure to Fla affected the backbone structure of SOD and CAT molecules and resulted in the formation of Fla-SOD polymers as well as an overall reduction in the size of the Fla-CAT binding system. Fla altered the microenvironment around Tyr residues in the SOD molecule and quenched the endogenous fluorescence of Tyr within the CAT molecule. In earthworm coelomocytes, Fla at 60 and 80 μM resulted in a significant elevation of CAT and SOD activities by 114% (
p
= 0.032) and 6.09% (
p
= 0.013), respectively. Molecular simulation results suggested that Fla-induced changes in the structure and conformation of SOD and CAT may be the key reason for their altered activities. The related redox homeostasis detection in earthworm coelomocytes indicated that high concentrations (80 μM) of Fla led to a significant accumulation of intracellular ROS (
p
= 0.018) and resulted in the development of lipid peroxidation. Our work contributes to an in-depth understanding of the biological effect of Fla to sensitive soil fauna, thus providing new ideas for Fla ecological risk prevention and control.
Graphical Abstract |
| doi_str_mv | 10.1007/s11356-023-29703-2 |
| format | Article |
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Eisenia fetida
) coelomocytes based on the molecular-level results. Our results showed that the exposure to Fla affected the backbone structure of SOD and CAT molecules and resulted in the formation of Fla-SOD polymers as well as an overall reduction in the size of the Fla-CAT binding system. Fla altered the microenvironment around Tyr residues in the SOD molecule and quenched the endogenous fluorescence of Tyr within the CAT molecule. In earthworm coelomocytes, Fla at 60 and 80 μM resulted in a significant elevation of CAT and SOD activities by 114% (
p
= 0.032) and 6.09% (
p
= 0.013), respectively. Molecular simulation results suggested that Fla-induced changes in the structure and conformation of SOD and CAT may be the key reason for their altered activities. The related redox homeostasis detection in earthworm coelomocytes indicated that high concentrations (80 μM) of Fla led to a significant accumulation of intracellular ROS (
p
= 0.018) and resulted in the development of lipid peroxidation. Our work contributes to an in-depth understanding of the biological effect of Fla to sensitive soil fauna, thus providing new ideas for Fla ecological risk prevention and control.
Graphical Abstract</description><identifier>ISSN: 1614-7499</identifier><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-023-29703-2</identifier><identifier>PMID: 37698795</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; antioxidants ; Antioxidants - metabolism ; Aquatic Pollution ; Aromatic hydrocarbons ; Atmospheric Protection/Air Quality Control/Air Pollution ; bioactive properties ; Biological effects ; Biota ; Catalase ; Catalase - metabolism ; Coelomocytes ; Conformation ; Earth and Environmental Science ; earthworms ; Ecotoxicology ; Eisenia fetida ; Environment ; Environmental Chemistry ; Environmental Health ; Fluoranthene ; fluorescence ; Homeostasis ; Industrial areas ; intermediate product ; Lipid peroxidation ; Lipids ; Malondialdehyde - metabolism ; Microenvironments ; molecular dynamics ; Molecular interactions ; Molecular modelling ; Molecular structure ; oil and gas industry ; Oligochaeta ; Oxidative Stress ; Peroxidation ; Petrochemicals ; Petrochemicals industry ; Polycyclic aromatic hydrocarbons ; Polymers ; Raw materials ; Research Article ; risk ; Risk management ; Soil - chemistry ; Soil fauna ; Soil Pollutants - metabolism ; Soils ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Topsoil ; Waste Water Technology ; Water Management ; Water Pollution Control ; Worms</subject><ispartof>Environmental science and pollution research international, 2023-10, Vol.30 (47), p.104233-104245</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-20a8b1c81a9fd81664700ed5b9376006ec8c87ff5cbba15e6b3cba0923a15e953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11356-023-29703-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11356-023-29703-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37698795$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Yan</creatorcontrib><creatorcontrib>Sun, Kailun</creatorcontrib><creatorcontrib>Zhao, Qiang</creatorcontrib><creatorcontrib>Li, Yuze</creatorcontrib><creatorcontrib>Liu, Guiqing</creatorcontrib><creatorcontrib>Liu, Rutao</creatorcontrib><title>Molecular interaction mechanisms and cellular response of superoxide dismutase and catalase to fluoranthene</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>As an important raw material and intermediate product of the petrochemical industry, fluoranthene (Fla) can be emitted with industrial activities and has become a typical polycyclic aromatic hydrocarbon enriched in the Chinese topsoil layer, posing a significant threat to sensitive soil biota. Here, multispectral tools and molecular simulation techniques were integrated to elucidate the molecular mechanism of Fla interaction with key antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) at the molecular level. Meanwhile, we further revealed the cellular responses of SOD and CAT and the associated redox states in earthworm (
Eisenia fetida
) coelomocytes based on the molecular-level results. Our results showed that the exposure to Fla affected the backbone structure of SOD and CAT molecules and resulted in the formation of Fla-SOD polymers as well as an overall reduction in the size of the Fla-CAT binding system. Fla altered the microenvironment around Tyr residues in the SOD molecule and quenched the endogenous fluorescence of Tyr within the CAT molecule. In earthworm coelomocytes, Fla at 60 and 80 μM resulted in a significant elevation of CAT and SOD activities by 114% (
p
= 0.032) and 6.09% (
p
= 0.013), respectively. Molecular simulation results suggested that Fla-induced changes in the structure and conformation of SOD and CAT may be the key reason for their altered activities. The related redox homeostasis detection in earthworm coelomocytes indicated that high concentrations (80 μM) of Fla led to a significant accumulation of intracellular ROS (
p
= 0.018) and resulted in the development of lipid peroxidation. Our work contributes to an in-depth understanding of the biological effect of Fla to sensitive soil fauna, thus providing new ideas for Fla ecological risk prevention and control.
Graphical Abstract</description><subject>Animals</subject><subject>antioxidants</subject><subject>Antioxidants - metabolism</subject><subject>Aquatic Pollution</subject><subject>Aromatic hydrocarbons</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>bioactive properties</subject><subject>Biological effects</subject><subject>Biota</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Coelomocytes</subject><subject>Conformation</subject><subject>Earth and Environmental Science</subject><subject>earthworms</subject><subject>Ecotoxicology</subject><subject>Eisenia fetida</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Fluoranthene</subject><subject>fluorescence</subject><subject>Homeostasis</subject><subject>Industrial areas</subject><subject>intermediate product</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Malondialdehyde - metabolism</subject><subject>Microenvironments</subject><subject>molecular dynamics</subject><subject>Molecular interactions</subject><subject>Molecular modelling</subject><subject>Molecular structure</subject><subject>oil and gas industry</subject><subject>Oligochaeta</subject><subject>Oxidative Stress</subject><subject>Peroxidation</subject><subject>Petrochemicals</subject><subject>Petrochemicals industry</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>Polymers</subject><subject>Raw materials</subject><subject>Research Article</subject><subject>risk</subject><subject>Risk management</subject><subject>Soil - chemistry</subject><subject>Soil fauna</subject><subject>Soil Pollutants - metabolism</subject><subject>Soils</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Topsoil</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><subject>Worms</subject><issn>1614-7499</issn><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkUlPxSAUhYnROP8BF6aJGzdVhgJlaYxT8owbXRNKb319tvCENtF_L29wiAvdABe-ey4nB6Ejgs8IxvI8EsK4yDFlOVUSp3UD7RJBilwWSm3-OO-gvRhnGFOsqNxGO0wKVUrFd9HLve_Ajp0JWesGCMYOrXdZD3ZqXBv7mBlXZxa6bskEiHPvImS-yeI4h-Df2hqyOpHjYNL9kjaD6RbF4LOmG30wbpiCgwO01ZguwuF630dP11ePl7f55OHm7vJiklvG1ZBTbMqK2JIY1dQlEaKQGEPNK5W-jbEAW9pSNg23VWUIB1ExW5nkjC0qxdk-Ol3pzoN_HSEOum_jwoJx4MeoGeGMS8pw-S9KS1EQLhTFCT35hc78GFwykijJRVEwThJFV5QNPsYAjZ6HtjfhXROsF6npVWo6paaXqWmamo7X0mPVQ_3V8hlTAtgKiOnJPUP4nv2H7AdGtaOX</recordid><startdate>20231001</startdate><enddate>20231001</enddate><creator>Song, Yan</creator><creator>Sun, Kailun</creator><creator>Zhao, Qiang</creator><creator>Li, Yuze</creator><creator>Liu, Guiqing</creator><creator>Liu, Rutao</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7TV</scope><scope>7U7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20231001</creationdate><title>Molecular interaction mechanisms and cellular response of superoxide dismutase and catalase to fluoranthene</title><author>Song, Yan ; Sun, Kailun ; Zhao, Qiang ; Li, Yuze ; Liu, Guiqing ; Liu, Rutao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-20a8b1c81a9fd81664700ed5b9376006ec8c87ff5cbba15e6b3cba0923a15e953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>antioxidants</topic><topic>Antioxidants - metabolism</topic><topic>Aquatic Pollution</topic><topic>Aromatic hydrocarbons</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>bioactive properties</topic><topic>Biological effects</topic><topic>Biota</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Coelomocytes</topic><topic>Conformation</topic><topic>Earth and Environmental Science</topic><topic>earthworms</topic><topic>Ecotoxicology</topic><topic>Eisenia fetida</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Fluoranthene</topic><topic>fluorescence</topic><topic>Homeostasis</topic><topic>Industrial areas</topic><topic>intermediate product</topic><topic>Lipid peroxidation</topic><topic>Lipids</topic><topic>Malondialdehyde - metabolism</topic><topic>Microenvironments</topic><topic>molecular dynamics</topic><topic>Molecular interactions</topic><topic>Molecular modelling</topic><topic>Molecular structure</topic><topic>oil and gas industry</topic><topic>Oligochaeta</topic><topic>Oxidative Stress</topic><topic>Peroxidation</topic><topic>Petrochemicals</topic><topic>Petrochemicals industry</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>Polymers</topic><topic>Raw materials</topic><topic>Research Article</topic><topic>risk</topic><topic>Risk management</topic><topic>Soil - chemistry</topic><topic>Soil fauna</topic><topic>Soil Pollutants - metabolism</topic><topic>Soils</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Topsoil</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Yan</creatorcontrib><creatorcontrib>Sun, Kailun</creatorcontrib><creatorcontrib>Zhao, Qiang</creatorcontrib><creatorcontrib>Li, Yuze</creatorcontrib><creatorcontrib>Liu, Guiqing</creatorcontrib><creatorcontrib>Liu, Rutao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Yan</au><au>Sun, Kailun</au><au>Zhao, Qiang</au><au>Li, Yuze</au><au>Liu, Guiqing</au><au>Liu, Rutao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular interaction mechanisms and cellular response of superoxide dismutase and catalase to fluoranthene</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2023-10-01</date><risdate>2023</risdate><volume>30</volume><issue>47</issue><spage>104233</spage><epage>104245</epage><pages>104233-104245</pages><issn>1614-7499</issn><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>As an important raw material and intermediate product of the petrochemical industry, fluoranthene (Fla) can be emitted with industrial activities and has become a typical polycyclic aromatic hydrocarbon enriched in the Chinese topsoil layer, posing a significant threat to sensitive soil biota. Here, multispectral tools and molecular simulation techniques were integrated to elucidate the molecular mechanism of Fla interaction with key antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) at the molecular level. Meanwhile, we further revealed the cellular responses of SOD and CAT and the associated redox states in earthworm (
Eisenia fetida
) coelomocytes based on the molecular-level results. Our results showed that the exposure to Fla affected the backbone structure of SOD and CAT molecules and resulted in the formation of Fla-SOD polymers as well as an overall reduction in the size of the Fla-CAT binding system. Fla altered the microenvironment around Tyr residues in the SOD molecule and quenched the endogenous fluorescence of Tyr within the CAT molecule. In earthworm coelomocytes, Fla at 60 and 80 μM resulted in a significant elevation of CAT and SOD activities by 114% (
p
= 0.032) and 6.09% (
p
= 0.013), respectively. Molecular simulation results suggested that Fla-induced changes in the structure and conformation of SOD and CAT may be the key reason for their altered activities. The related redox homeostasis detection in earthworm coelomocytes indicated that high concentrations (80 μM) of Fla led to a significant accumulation of intracellular ROS (
p
= 0.018) and resulted in the development of lipid peroxidation. Our work contributes to an in-depth understanding of the biological effect of Fla to sensitive soil fauna, thus providing new ideas for Fla ecological risk prevention and control.
Graphical Abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37698795</pmid><doi>10.1007/s11356-023-29703-2</doi><tpages>13</tpages></addata></record> |
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| subjects | Animals antioxidants Antioxidants - metabolism Aquatic Pollution Aromatic hydrocarbons Atmospheric Protection/Air Quality Control/Air Pollution bioactive properties Biological effects Biota Catalase Catalase - metabolism Coelomocytes Conformation Earth and Environmental Science earthworms Ecotoxicology Eisenia fetida Environment Environmental Chemistry Environmental Health Fluoranthene fluorescence Homeostasis Industrial areas intermediate product Lipid peroxidation Lipids Malondialdehyde - metabolism Microenvironments molecular dynamics Molecular interactions Molecular modelling Molecular structure oil and gas industry Oligochaeta Oxidative Stress Peroxidation Petrochemicals Petrochemicals industry Polycyclic aromatic hydrocarbons Polymers Raw materials Research Article risk Risk management Soil - chemistry Soil fauna Soil Pollutants - metabolism Soils Superoxide dismutase Superoxide Dismutase - metabolism Topsoil Waste Water Technology Water Management Water Pollution Control Worms |
| title | Molecular interaction mechanisms and cellular response of superoxide dismutase and catalase to fluoranthene |
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