Detection of high-risk human papillomavirus infected cervical biopsies samples by immunohistochemical expression of the p16 tumor marker
Cervical cancer is the fourth most common type of cancer in women worldwide. It is widely accepted that the main cause of cervical cancer, especially in underdeveloped countries like Pakistan, is the infection caused by the human papillomavirus (HPV). The current screening and diagnostic methods fac...
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| Veröffentlicht in: | Archives of microbiology 2024-01, Vol.206 (1), p.17-17, Article 17 |
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| creator | Shafique, Muhammad Shoaib, Iqra Aslam, Bilal Khalid, Rabia Tanvir, Imrana Rasool, Muhammad Hidayat Shah, Tawaf Ali Almaary, Khalid S. Bourhia, Mohammed Qamar, Muhammad Usman |
| description | Cervical cancer is the fourth most common type of cancer in women worldwide. It is widely accepted that the main cause of cervical cancer, especially in underdeveloped countries like Pakistan, is the infection caused by the human papillomavirus (HPV). The current screening and diagnostic methods face several challenges in accurately detecting the various types of lesions caused by HPV. Therefore, the present study was conducted to assess the effectiveness of p16 immunohistochemistry (IHC) analysis as a diagnostic method in samples of cervical biopsies. One hundred cervical biopsy samples were obtained from female patients across various age groups (> 20– ≤ 30, > 31– ≤ 40, > 41– ≤ 50, > 51– ≤ 60 years). These samples were subsequently prepared for subsequent examination. All samples were analyzed using automated tissue processing followed by Hematoxylin and Eosin (H & E) staining, and p16 IHC tumour marker staining. The H & E slides showed changes in normal cervical tissues, while four cervical abnormalities were identified statistically significant using p16 marker including chronic cervicitis, nabothian cyst formation, cervical intraepithelial neoplasia, and cervical cancers (
P value
0.014). Furthermore, among females of different age groups (> 31– ≤ 40, > 41– ≤ 50, > 51– ≤ 60 years) were found statistically significant suffering from cervical cancer (
P value
0.04), HPV with cervical cancer (
P value
0.01), HPV with cervical intraepithelial neoplasia (
P value
0.01). Based on the available data, it can be inferred that the incorporation of the p16 tumor marker may be a valuable method for detecting high-risk HPV in cervical biopsies samples. |
| doi_str_mv | 10.1007/s00203-023-03736-0 |
| format | Article |
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P value
0.014). Furthermore, among females of different age groups (> 31– ≤ 40, > 41– ≤ 50, > 51– ≤ 60 years) were found statistically significant suffering from cervical cancer (
P value
0.04), HPV with cervical cancer (
P value
0.01), HPV with cervical intraepithelial neoplasia (
P value
0.01). Based on the available data, it can be inferred that the incorporation of the p16 tumor marker may be a valuable method for detecting high-risk HPV in cervical biopsies samples.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-023-03736-0</identifier><identifier>PMID: 38085340</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abnormalities ; Adult ; Age groups ; automation ; Biochemistry ; Biomarkers ; Biomarkers, Tumor - analysis ; Biomarkers, Tumor - metabolism ; Biomedical and Life Sciences ; Biopsy ; Biotechnology ; Cancer ; Cell Biology ; Cervical cancer ; Cervicitis ; Developing countries ; Diagnostic systems ; diagnostic techniques ; Ecology ; eosin ; Female ; Females ; Human papillomavirus ; Human Papillomavirus Viruses ; Humans ; Immunohistochemistry ; LDCs ; Life Sciences ; Microbial Ecology ; Microbiology ; Middle Aged ; Original Paper ; Pakistan ; Papillomaviridae ; Papillomavirus Infections - diagnosis ; Papillomavirus Infections - pathology ; Samples ; Staining ; Statistical analysis ; Tumor markers ; Tumors ; Uterine Cervical Dysplasia - diagnosis ; Uterine Cervical Dysplasia - pathology ; uterine cervical neoplasms ; Uterine Cervical Neoplasms - diagnosis ; Uterine Cervical Neoplasms - pathology ; Young Adult</subject><ispartof>Archives of microbiology, 2024-01, Vol.206 (1), p.17-17, Article 17</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-5b52a5ebca12de14b15706ea298309ea3c1408d07a80e5c5bcb7360c80b0b4763</citedby><cites>FETCH-LOGICAL-c408t-5b52a5ebca12de14b15706ea298309ea3c1408d07a80e5c5bcb7360c80b0b4763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-023-03736-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-023-03736-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38085340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shafique, Muhammad</creatorcontrib><creatorcontrib>Shoaib, Iqra</creatorcontrib><creatorcontrib>Aslam, Bilal</creatorcontrib><creatorcontrib>Khalid, Rabia</creatorcontrib><creatorcontrib>Tanvir, Imrana</creatorcontrib><creatorcontrib>Rasool, Muhammad Hidayat</creatorcontrib><creatorcontrib>Shah, Tawaf Ali</creatorcontrib><creatorcontrib>Almaary, Khalid S.</creatorcontrib><creatorcontrib>Bourhia, Mohammed</creatorcontrib><creatorcontrib>Qamar, Muhammad Usman</creatorcontrib><title>Detection of high-risk human papillomavirus infected cervical biopsies samples by immunohistochemical expression of the p16 tumor marker</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Cervical cancer is the fourth most common type of cancer in women worldwide. It is widely accepted that the main cause of cervical cancer, especially in underdeveloped countries like Pakistan, is the infection caused by the human papillomavirus (HPV). The current screening and diagnostic methods face several challenges in accurately detecting the various types of lesions caused by HPV. Therefore, the present study was conducted to assess the effectiveness of p16 immunohistochemistry (IHC) analysis as a diagnostic method in samples of cervical biopsies. One hundred cervical biopsy samples were obtained from female patients across various age groups (> 20– ≤ 30, > 31– ≤ 40, > 41– ≤ 50, > 51– ≤ 60 years). These samples were subsequently prepared for subsequent examination. All samples were analyzed using automated tissue processing followed by Hematoxylin and Eosin (H & E) staining, and p16 IHC tumour marker staining. The H & E slides showed changes in normal cervical tissues, while four cervical abnormalities were identified statistically significant using p16 marker including chronic cervicitis, nabothian cyst formation, cervical intraepithelial neoplasia, and cervical cancers (
P value
0.014). Furthermore, among females of different age groups (> 31– ≤ 40, > 41– ≤ 50, > 51– ≤ 60 years) were found statistically significant suffering from cervical cancer (
P value
0.04), HPV with cervical cancer (
P value
0.01), HPV with cervical intraepithelial neoplasia (
P value
0.01). Based on the available data, it can be inferred that the incorporation of the p16 tumor marker may be a valuable method for detecting high-risk HPV in cervical biopsies samples.</description><subject>Abnormalities</subject><subject>Adult</subject><subject>Age groups</subject><subject>automation</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biopsy</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Cell Biology</subject><subject>Cervical cancer</subject><subject>Cervicitis</subject><subject>Developing countries</subject><subject>Diagnostic systems</subject><subject>diagnostic techniques</subject><subject>Ecology</subject><subject>eosin</subject><subject>Female</subject><subject>Females</subject><subject>Human papillomavirus</subject><subject>Human Papillomavirus Viruses</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>LDCs</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Original Paper</subject><subject>Pakistan</subject><subject>Papillomaviridae</subject><subject>Papillomavirus Infections - diagnosis</subject><subject>Papillomavirus Infections - pathology</subject><subject>Samples</subject><subject>Staining</subject><subject>Statistical analysis</subject><subject>Tumor markers</subject><subject>Tumors</subject><subject>Uterine Cervical Dysplasia - diagnosis</subject><subject>Uterine Cervical Dysplasia - pathology</subject><subject>uterine cervical neoplasms</subject><subject>Uterine Cervical Neoplasms - diagnosis</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Young Adult</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc9u1DAQhy0EosvCC3BAlrhwCYztOHGOqPyVKnEBiVtke2cbt3EcPElF34DHxttdQOIAB2sO_n4zHn-MPRXwUgC0rwhAgqpAlqNa1VRwj21ErWQFrfx6n21AgaxMp9QZe0R0BSCkMeYhO1MGjFY1bNiPN7igX0KaeNrzIVwOVQ50zYc12onPdg7jmKK9CXklHqZ9YXHHPeab4O3IXUgzBSRONs5jqe6WhxjXKQ2BluQHjHccfp8zEp3GLAPyWTR8WWPKPNp8jfkxe7C3I-GTU92yL-_efj7_UF18ev_x_PVF5WswS6Wdllaj81bIHYraCd1Cg1Z2RkGHVnlRuB201gBqr5135WfAG3Dg6rZRW_bi2HfO6duKtPQxkMdxtBOmlXoltNKNEbX5Lyo7kJ2q65LYsud_oVdpzVNZ5EBBW95QVG2ZPFI-J6KM-37Ooax_2wvoD0r7o9K-KO3vlPaH0LNT69VF3P2O_HJYAHUEqFxNl5j_zP5H25_Oa62N</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Shafique, Muhammad</creator><creator>Shoaib, Iqra</creator><creator>Aslam, Bilal</creator><creator>Khalid, Rabia</creator><creator>Tanvir, Imrana</creator><creator>Rasool, Muhammad Hidayat</creator><creator>Shah, Tawaf Ali</creator><creator>Almaary, Khalid S.</creator><creator>Bourhia, Mohammed</creator><creator>Qamar, Muhammad Usman</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240101</creationdate><title>Detection of high-risk human papillomavirus infected cervical biopsies samples by immunohistochemical expression of the p16 tumor marker</title><author>Shafique, Muhammad ; Shoaib, Iqra ; Aslam, Bilal ; Khalid, Rabia ; Tanvir, Imrana ; Rasool, Muhammad Hidayat ; Shah, Tawaf Ali ; Almaary, Khalid S. ; Bourhia, Mohammed ; Qamar, Muhammad Usman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-5b52a5ebca12de14b15706ea298309ea3c1408d07a80e5c5bcb7360c80b0b4763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Abnormalities</topic><topic>Adult</topic><topic>Age groups</topic><topic>automation</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biopsy</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Cell Biology</topic><topic>Cervical cancer</topic><topic>Cervicitis</topic><topic>Developing countries</topic><topic>Diagnostic systems</topic><topic>diagnostic techniques</topic><topic>Ecology</topic><topic>eosin</topic><topic>Female</topic><topic>Females</topic><topic>Human papillomavirus</topic><topic>Human Papillomavirus Viruses</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>LDCs</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Original Paper</topic><topic>Pakistan</topic><topic>Papillomaviridae</topic><topic>Papillomavirus Infections - diagnosis</topic><topic>Papillomavirus Infections - pathology</topic><topic>Samples</topic><topic>Staining</topic><topic>Statistical analysis</topic><topic>Tumor markers</topic><topic>Tumors</topic><topic>Uterine Cervical Dysplasia - diagnosis</topic><topic>Uterine Cervical Dysplasia - pathology</topic><topic>uterine cervical neoplasms</topic><topic>Uterine Cervical Neoplasms - diagnosis</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shafique, Muhammad</creatorcontrib><creatorcontrib>Shoaib, Iqra</creatorcontrib><creatorcontrib>Aslam, Bilal</creatorcontrib><creatorcontrib>Khalid, Rabia</creatorcontrib><creatorcontrib>Tanvir, Imrana</creatorcontrib><creatorcontrib>Rasool, Muhammad Hidayat</creatorcontrib><creatorcontrib>Shah, Tawaf Ali</creatorcontrib><creatorcontrib>Almaary, Khalid S.</creatorcontrib><creatorcontrib>Bourhia, Mohammed</creatorcontrib><creatorcontrib>Qamar, Muhammad Usman</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shafique, Muhammad</au><au>Shoaib, Iqra</au><au>Aslam, Bilal</au><au>Khalid, Rabia</au><au>Tanvir, Imrana</au><au>Rasool, Muhammad Hidayat</au><au>Shah, Tawaf Ali</au><au>Almaary, Khalid S.</au><au>Bourhia, Mohammed</au><au>Qamar, Muhammad Usman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of high-risk human papillomavirus infected cervical biopsies samples by immunohistochemical expression of the p16 tumor marker</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>206</volume><issue>1</issue><spage>17</spage><epage>17</epage><pages>17-17</pages><artnum>17</artnum><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Cervical cancer is the fourth most common type of cancer in women worldwide. It is widely accepted that the main cause of cervical cancer, especially in underdeveloped countries like Pakistan, is the infection caused by the human papillomavirus (HPV). The current screening and diagnostic methods face several challenges in accurately detecting the various types of lesions caused by HPV. Therefore, the present study was conducted to assess the effectiveness of p16 immunohistochemistry (IHC) analysis as a diagnostic method in samples of cervical biopsies. One hundred cervical biopsy samples were obtained from female patients across various age groups (> 20– ≤ 30, > 31– ≤ 40, > 41– ≤ 50, > 51– ≤ 60 years). These samples were subsequently prepared for subsequent examination. All samples were analyzed using automated tissue processing followed by Hematoxylin and Eosin (H & E) staining, and p16 IHC tumour marker staining. The H & E slides showed changes in normal cervical tissues, while four cervical abnormalities were identified statistically significant using p16 marker including chronic cervicitis, nabothian cyst formation, cervical intraepithelial neoplasia, and cervical cancers (
P value
0.014). Furthermore, among females of different age groups (> 31– ≤ 40, > 41– ≤ 50, > 51– ≤ 60 years) were found statistically significant suffering from cervical cancer (
P value
0.04), HPV with cervical cancer (
P value
0.01), HPV with cervical intraepithelial neoplasia (
P value
0.01). Based on the available data, it can be inferred that the incorporation of the p16 tumor marker may be a valuable method for detecting high-risk HPV in cervical biopsies samples.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38085340</pmid><doi>10.1007/s00203-023-03736-0</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-8933 |
| ispartof | Archives of microbiology, 2024-01, Vol.206 (1), p.17-17, Article 17 |
| issn | 0302-8933 1432-072X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153568148 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Abnormalities Adult Age groups automation Biochemistry Biomarkers Biomarkers, Tumor - analysis Biomarkers, Tumor - metabolism Biomedical and Life Sciences Biopsy Biotechnology Cancer Cell Biology Cervical cancer Cervicitis Developing countries Diagnostic systems diagnostic techniques Ecology eosin Female Females Human papillomavirus Human Papillomavirus Viruses Humans Immunohistochemistry LDCs Life Sciences Microbial Ecology Microbiology Middle Aged Original Paper Pakistan Papillomaviridae Papillomavirus Infections - diagnosis Papillomavirus Infections - pathology Samples Staining Statistical analysis Tumor markers Tumors Uterine Cervical Dysplasia - diagnosis Uterine Cervical Dysplasia - pathology uterine cervical neoplasms Uterine Cervical Neoplasms - diagnosis Uterine Cervical Neoplasms - pathology Young Adult |
| title | Detection of high-risk human papillomavirus infected cervical biopsies samples by immunohistochemical expression of the p16 tumor marker |
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