Fast and Precise Temperature Control for Axon Stretch Growth Bioreactor Based on Fuzzy PID Control
A suitable environment is essential for successful long-term cell culturing in vitro. Too high or too low temperature will affect the growth of cells, so we need to maintain the constant temperature of the cell culture environment. Usually, cells are cultured in a cell incubator, and the constant te...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2023-12, Vol.195 (12), p.7446-7464 |
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| creator | Li, Xiao Dong, Xianxin Wang, Jun Tu, Xikai Huang, Hailong Cao, Yuanpeng Wang, Chenlin Huang, Yizhe |
| description | A suitable environment is essential for successful long-term cell culturing in vitro. Too high or too low temperature will affect the growth of cells, so we need to maintain the constant temperature of the cell culture environment. Usually, cells are cultured in a cell incubator, and the constant temperature is provided by the cell incubator. Recently, we have developed a multi-channel axon stretch growth bioreactor for rapid acquisition of autologous nerve tissue. Since the motor and controller are placed in the incubator for a long time, the service life of the equipment will be shortened or even damaged due to high humidity and weak acid environment. In order to enable the axon stretch growth bioreactor to culture cells independently, we designed a constant temperature control system for the device. Firstly, the simulation results show that the fuzzy PID control reduces the overshoot and improves the traditional PID control with large overshoot and low control precision. Then, the two control algorithms were applied to the multi-channel axon stretch growth bioreactor by STM32F4 microcontroller. The experimental data show that the fuzzy PID control algorithm has good control effect and can meet the requirement of constant temperature of cell growth. Finally, nerve cells derived from human pluripotent stem cells were successfully cultured in a cell culture amplification chamber under a constant temperature environment provided by a fuzzy PID controller, and well-developed axons could be seen. In the future, we may transplant stretch growth axons into living organisms to repair nerve damage. |
| doi_str_mv | 10.1007/s12010-023-04449-2 |
| format | Article |
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Too high or too low temperature will affect the growth of cells, so we need to maintain the constant temperature of the cell culture environment. Usually, cells are cultured in a cell incubator, and the constant temperature is provided by the cell incubator. Recently, we have developed a multi-channel axon stretch growth bioreactor for rapid acquisition of autologous nerve tissue. Since the motor and controller are placed in the incubator for a long time, the service life of the equipment will be shortened or even damaged due to high humidity and weak acid environment. In order to enable the axon stretch growth bioreactor to culture cells independently, we designed a constant temperature control system for the device. Firstly, the simulation results show that the fuzzy PID control reduces the overshoot and improves the traditional PID control with large overshoot and low control precision. Then, the two control algorithms were applied to the multi-channel axon stretch growth bioreactor by STM32F4 microcontroller. The experimental data show that the fuzzy PID control algorithm has good control effect and can meet the requirement of constant temperature of cell growth. Finally, nerve cells derived from human pluripotent stem cells were successfully cultured in a cell culture amplification chamber under a constant temperature environment provided by a fuzzy PID controller, and well-developed axons could be seen. In the future, we may transplant stretch growth axons into living organisms to repair nerve damage.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-023-04449-2</identifier><identifier>PMID: 37004648</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Algorithms ; Axonogenesis ; Axons ; Axons - physiology ; Biochemistry ; Bioreactors ; Biotechnology ; Cell culture ; cell growth ; Chemistry ; Chemistry and Materials Science ; Computer Simulation ; Control algorithms ; Control systems ; Control theory ; Controllers ; Damage ; durability ; Fuzzy control ; Humans ; humidity ; Low temperature ; nerve tissue ; Nerves ; Nervous tissues ; Original Article ; Pluripotency ; Proportional integral derivative ; Service life ; Stem cells ; Temperature ; Temperature control ; Temperature requirements</subject><ispartof>Applied biochemistry and biotechnology, 2023-12, Vol.195 (12), p.7446-7464</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. 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Too high or too low temperature will affect the growth of cells, so we need to maintain the constant temperature of the cell culture environment. Usually, cells are cultured in a cell incubator, and the constant temperature is provided by the cell incubator. Recently, we have developed a multi-channel axon stretch growth bioreactor for rapid acquisition of autologous nerve tissue. Since the motor and controller are placed in the incubator for a long time, the service life of the equipment will be shortened or even damaged due to high humidity and weak acid environment. In order to enable the axon stretch growth bioreactor to culture cells independently, we designed a constant temperature control system for the device. Firstly, the simulation results show that the fuzzy PID control reduces the overshoot and improves the traditional PID control with large overshoot and low control precision. Then, the two control algorithms were applied to the multi-channel axon stretch growth bioreactor by STM32F4 microcontroller. The experimental data show that the fuzzy PID control algorithm has good control effect and can meet the requirement of constant temperature of cell growth. Finally, nerve cells derived from human pluripotent stem cells were successfully cultured in a cell culture amplification chamber under a constant temperature environment provided by a fuzzy PID controller, and well-developed axons could be seen. In the future, we may transplant stretch growth axons into living organisms to repair nerve damage.</description><subject>Algorithms</subject><subject>Axonogenesis</subject><subject>Axons</subject><subject>Axons - physiology</subject><subject>Biochemistry</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>cell growth</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Computer Simulation</subject><subject>Control algorithms</subject><subject>Control systems</subject><subject>Control theory</subject><subject>Controllers</subject><subject>Damage</subject><subject>durability</subject><subject>Fuzzy control</subject><subject>Humans</subject><subject>humidity</subject><subject>Low temperature</subject><subject>nerve tissue</subject><subject>Nerves</subject><subject>Nervous tissues</subject><subject>Original Article</subject><subject>Pluripotency</subject><subject>Proportional integral derivative</subject><subject>Service life</subject><subject>Stem cells</subject><subject>Temperature</subject><subject>Temperature control</subject><subject>Temperature requirements</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU1LXDEUhkNRdLT-gS5KwE03tz35TpY67VhBqFC7DrlJbh2ZuRmTXFr99Y2OttCFrg7kPO97CA9C7wh8JADqUyEUCHRAWQecc9PRN2hGhDDtyZAdNAOqWEepNvvooJQbAEK1UHtonykALrmeoX7hSsVuDPgyR78sEV_F9SZmV6cc8TyNNacVHlLGJ7_TiL_XHKu_xmc5_arX-HSZcnS-tvWpKzHghiym-_s7fHn--Tn9Fu0OblXi0dM8RD8WX67mX7uLb2fn85OLznPQtRMmgNQKtPaS834IgXlGnHESiGSmH0QggRMRvBAycKq81rL3EBTjHARnh-jDtneT0-0US7XrZfFxtXJjTFOxjAgmpGIAr6JUGS61YVI09Pg_9CZNeWwfsdSAAsq1fiikW8rnVEqOg93k5drlO0vAPsiyW1m2ybKPsixtofdP1VO_juFv5NlOA9gWKG01_oz53-0Xav8AjGqcwA</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Li, Xiao</creator><creator>Dong, Xianxin</creator><creator>Wang, Jun</creator><creator>Tu, Xikai</creator><creator>Huang, Hailong</creator><creator>Cao, Yuanpeng</creator><creator>Wang, Chenlin</creator><creator>Huang, Yizhe</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20231201</creationdate><title>Fast and Precise Temperature Control for Axon Stretch Growth Bioreactor Based on Fuzzy PID Control</title><author>Li, Xiao ; Dong, Xianxin ; Wang, Jun ; Tu, Xikai ; Huang, Hailong ; Cao, Yuanpeng ; Wang, Chenlin ; Huang, Yizhe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-59d0687088c644bfdd3c31a9a601639bf5d1d415dc556d427c886bc0d73440543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Algorithms</topic><topic>Axonogenesis</topic><topic>Axons</topic><topic>Axons - 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Too high or too low temperature will affect the growth of cells, so we need to maintain the constant temperature of the cell culture environment. Usually, cells are cultured in a cell incubator, and the constant temperature is provided by the cell incubator. Recently, we have developed a multi-channel axon stretch growth bioreactor for rapid acquisition of autologous nerve tissue. Since the motor and controller are placed in the incubator for a long time, the service life of the equipment will be shortened or even damaged due to high humidity and weak acid environment. In order to enable the axon stretch growth bioreactor to culture cells independently, we designed a constant temperature control system for the device. Firstly, the simulation results show that the fuzzy PID control reduces the overshoot and improves the traditional PID control with large overshoot and low control precision. Then, the two control algorithms were applied to the multi-channel axon stretch growth bioreactor by STM32F4 microcontroller. The experimental data show that the fuzzy PID control algorithm has good control effect and can meet the requirement of constant temperature of cell growth. Finally, nerve cells derived from human pluripotent stem cells were successfully cultured in a cell culture amplification chamber under a constant temperature environment provided by a fuzzy PID controller, and well-developed axons could be seen. In the future, we may transplant stretch growth axons into living organisms to repair nerve damage.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>37004648</pmid><doi>10.1007/s12010-023-04449-2</doi><tpages>19</tpages></addata></record> |
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| subjects | Algorithms Axonogenesis Axons Axons - physiology Biochemistry Bioreactors Biotechnology Cell culture cell growth Chemistry Chemistry and Materials Science Computer Simulation Control algorithms Control systems Control theory Controllers Damage durability Fuzzy control Humans humidity Low temperature nerve tissue Nerves Nervous tissues Original Article Pluripotency Proportional integral derivative Service life Stem cells Temperature Temperature control Temperature requirements |
| title | Fast and Precise Temperature Control for Axon Stretch Growth Bioreactor Based on Fuzzy PID Control |
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