Melatonin application during cryopreservation improves the development and clinical outcomes of human vitrified–warmed oocytes
In this clinical study, we investigated the potential of melatonin (MT) supplementation in the freeze-thaw medium used for cryopreserved human oocytes. In total, 152 patients who underwent in vitro fertilization between January 2020 and December 2022 were included and categorized into different grou...
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| Veröffentlicht in: | Cryobiology 2024-06, Vol.115, p.104902-104902, Article 104902 |
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| container_title | Cryobiology |
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| creator | Zhang, Chao Yang, Dandan Ding, Ding Fan, Yongqi Yang, Han Wang, Jing Zou, Huijuan Rao, Bihua Wang, Qiushuang Ye, Tingting Yu, Min Zhang, Zhiguo |
| description | In this clinical study, we investigated the potential of melatonin (MT) supplementation in the freeze-thaw medium used for cryopreserved human oocytes. In total, 152 patients who underwent in vitro fertilization between January 2020 and December 2022 were included and categorized into different groups as follows: the donor group, comprising 108 patients who donated their oocytes, with 34 patients using a vitrification and warming medium supplemented with MT (D-MT subgroup) and 74 patients using conventional medium without MT (D-0 subgroup); and the autologous group, comprising 38 patients who used their own oocytes, with 19 patients using medium supplemented with MT (A-MT subgroup) and 19 patients using medium without MT (A-0 subgroup). After thawing, the surviving oocytes in the D-MT and A-MT subgroups and D-0 and A-0 subgroups were cultured in a fertilization media with and without 10−9 MMT for 2.5 h, respectively, followed by intracytoplasmic sperm injection insemination, embryo culture, and transfer. The survival, cleavage, high-quality embryo, clinical pregnancy, ongoing pregnancy, and implantation rates were significantly higher in the D-MT subgroup than in the D-0 subgroup (all P |
| doi_str_mv | 10.1016/j.cryobiol.2024.104902 |
| format | Article |
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•MT application in cryopreservation can enhance the development of freeze–thawed human oocytes and improve clinical outcomes.•This study suggest that heterogeneity issues do not need to be considered for oocyte donation therapy.•This study offers a promising solution regarding the cryopreservation of human oocytes.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/j.cryobiol.2024.104902</identifier><identifier>PMID: 38734365</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adult ; blastocyst ; Blastocyst - drug effects ; Clinical outcomes ; cryobiology ; Cryopreservation ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; Development ; embryo culture ; Embryo Culture Techniques - methods ; Embryo Transfer ; Female ; Fertilization in Vitro - methods ; freeze-thaw cycles ; Human oocyte ; Humans ; insemination ; intracytoplasmic sperm injection ; Melatonin ; Melatonin - pharmacology ; oocytes ; Oocytes - drug effects ; Pregnancy ; Pregnancy Rate ; Sperm Injections, Intracytoplasmic - methods ; vitrification ; Vitrification - drug effects</subject><ispartof>Cryobiology, 2024-06, Vol.115, p.104902-104902, Article 104902</ispartof><rights>2024 Society for Cryobiology</rights><rights>Copyright © 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c348t-d0963f38c883383b1f4ccb387a095de321faf25c958dcee7d463393eeb9bdd543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0011224024000579$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38734365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Chao</creatorcontrib><creatorcontrib>Yang, Dandan</creatorcontrib><creatorcontrib>Ding, Ding</creatorcontrib><creatorcontrib>Fan, Yongqi</creatorcontrib><creatorcontrib>Yang, Han</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Zou, Huijuan</creatorcontrib><creatorcontrib>Rao, Bihua</creatorcontrib><creatorcontrib>Wang, Qiushuang</creatorcontrib><creatorcontrib>Ye, Tingting</creatorcontrib><creatorcontrib>Yu, Min</creatorcontrib><creatorcontrib>Zhang, Zhiguo</creatorcontrib><title>Melatonin application during cryopreservation improves the development and clinical outcomes of human vitrified–warmed oocytes</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>In this clinical study, we investigated the potential of melatonin (MT) supplementation in the freeze-thaw medium used for cryopreserved human oocytes. In total, 152 patients who underwent in vitro fertilization between January 2020 and December 2022 were included and categorized into different groups as follows: the donor group, comprising 108 patients who donated their oocytes, with 34 patients using a vitrification and warming medium supplemented with MT (D-MT subgroup) and 74 patients using conventional medium without MT (D-0 subgroup); and the autologous group, comprising 38 patients who used their own oocytes, with 19 patients using medium supplemented with MT (A-MT subgroup) and 19 patients using medium without MT (A-0 subgroup). After thawing, the surviving oocytes in the D-MT and A-MT subgroups and D-0 and A-0 subgroups were cultured in a fertilization media with and without 10−9 MMT for 2.5 h, respectively, followed by intracytoplasmic sperm injection insemination, embryo culture, and transfer. The survival, cleavage, high-quality embryo, clinical pregnancy, ongoing pregnancy, and implantation rates were significantly higher in the D-MT subgroup than in the D-0 subgroup (all P < 0.05). Similarly, the survival, fertilization, high-quality embryo, and high-quality blastocyst rates were significantly higher in the A-MT subgroup than in the A-0 subgroup (all P < 0.05). These findings indicate that MT addition during cryopreservation can enhance the development of vitrified-warmed human oocytes and improve clinical outcomes.
•MT application in cryopreservation can enhance the development of freeze–thawed human oocytes and improve clinical outcomes.•This study suggest that heterogeneity issues do not need to be considered for oocyte donation therapy.•This study offers a promising solution regarding the cryopreservation of human oocytes.</description><subject>Adult</subject><subject>blastocyst</subject><subject>Blastocyst - drug effects</subject><subject>Clinical outcomes</subject><subject>cryobiology</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Development</subject><subject>embryo culture</subject><subject>Embryo Culture Techniques - methods</subject><subject>Embryo Transfer</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>freeze-thaw cycles</subject><subject>Human oocyte</subject><subject>Humans</subject><subject>insemination</subject><subject>intracytoplasmic sperm injection</subject><subject>Melatonin</subject><subject>Melatonin - pharmacology</subject><subject>oocytes</subject><subject>Oocytes - drug effects</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Sperm Injections, Intracytoplasmic - methods</subject><subject>vitrification</subject><subject>Vitrification - drug effects</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQhy0EotvCK1Q-csnWf-I0voEqCkhFXOBsOfaEeuXYwXaC9tZ34A15ErxKy7WnkUbfzE8zH0KXlOwpod3VYW_SMQ4u-j0jrK3NVhL2Au0okaRhXLKXaEcIpQ1jLTlD5zkfCCHdNW9fozPe18o7sUMPX8HrEoMLWM-zd0YXFwO2S3LhJz5lzAkypHXru2lOcYWMyz1gCyv4OE8QCtbBYuNdqAs8jksxcapUHPH9MumAV1eSGx3Yvw9_fus0gcUxmmOB_Aa9GrXP8PaxXqAftx-_33xu7r59-nLz4a4xvO1LY4ns-Mh70_ec93ygY2vMUO_QRAoLnNFRj0wYKXprAK5t23EuOcAgB2tFyy_Qu21vPeDXArmoyWUD3usAccmKU8FFJyTlz6NEcNlTJkVFuw01KeacYFRzcpNOR0WJOolSB_UkSp1EqU1UHbx8zFiG-o3_Y09mKvB-A6A-ZXWQVDYOggHrEpiibHTPZfwDKkGsoQ</recordid><startdate>202406</startdate><enddate>202406</enddate><creator>Zhang, Chao</creator><creator>Yang, Dandan</creator><creator>Ding, Ding</creator><creator>Fan, Yongqi</creator><creator>Yang, Han</creator><creator>Wang, Jing</creator><creator>Zou, Huijuan</creator><creator>Rao, Bihua</creator><creator>Wang, Qiushuang</creator><creator>Ye, Tingting</creator><creator>Yu, Min</creator><creator>Zhang, Zhiguo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>202406</creationdate><title>Melatonin application during cryopreservation improves the development and clinical outcomes of human vitrified–warmed oocytes</title><author>Zhang, Chao ; Yang, Dandan ; Ding, Ding ; Fan, Yongqi ; Yang, Han ; Wang, Jing ; Zou, Huijuan ; Rao, Bihua ; Wang, Qiushuang ; Ye, Tingting ; Yu, Min ; Zhang, Zhiguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-d0963f38c883383b1f4ccb387a095de321faf25c958dcee7d463393eeb9bdd543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adult</topic><topic>blastocyst</topic><topic>Blastocyst - drug effects</topic><topic>Clinical outcomes</topic><topic>cryobiology</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Development</topic><topic>embryo culture</topic><topic>Embryo Culture Techniques - methods</topic><topic>Embryo Transfer</topic><topic>Female</topic><topic>Fertilization in Vitro - methods</topic><topic>freeze-thaw cycles</topic><topic>Human oocyte</topic><topic>Humans</topic><topic>insemination</topic><topic>intracytoplasmic sperm injection</topic><topic>Melatonin</topic><topic>Melatonin - pharmacology</topic><topic>oocytes</topic><topic>Oocytes - drug effects</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Sperm Injections, Intracytoplasmic - methods</topic><topic>vitrification</topic><topic>Vitrification - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Chao</creatorcontrib><creatorcontrib>Yang, Dandan</creatorcontrib><creatorcontrib>Ding, Ding</creatorcontrib><creatorcontrib>Fan, Yongqi</creatorcontrib><creatorcontrib>Yang, Han</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Zou, Huijuan</creatorcontrib><creatorcontrib>Rao, Bihua</creatorcontrib><creatorcontrib>Wang, Qiushuang</creatorcontrib><creatorcontrib>Ye, Tingting</creatorcontrib><creatorcontrib>Yu, Min</creatorcontrib><creatorcontrib>Zhang, Zhiguo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Chao</au><au>Yang, Dandan</au><au>Ding, Ding</au><au>Fan, Yongqi</au><au>Yang, Han</au><au>Wang, Jing</au><au>Zou, Huijuan</au><au>Rao, Bihua</au><au>Wang, Qiushuang</au><au>Ye, Tingting</au><au>Yu, Min</au><au>Zhang, Zhiguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Melatonin application during cryopreservation improves the development and clinical outcomes of human vitrified–warmed oocytes</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>2024-06</date><risdate>2024</risdate><volume>115</volume><spage>104902</spage><epage>104902</epage><pages>104902-104902</pages><artnum>104902</artnum><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>In this clinical study, we investigated the potential of melatonin (MT) supplementation in the freeze-thaw medium used for cryopreserved human oocytes. In total, 152 patients who underwent in vitro fertilization between January 2020 and December 2022 were included and categorized into different groups as follows: the donor group, comprising 108 patients who donated their oocytes, with 34 patients using a vitrification and warming medium supplemented with MT (D-MT subgroup) and 74 patients using conventional medium without MT (D-0 subgroup); and the autologous group, comprising 38 patients who used their own oocytes, with 19 patients using medium supplemented with MT (A-MT subgroup) and 19 patients using medium without MT (A-0 subgroup). After thawing, the surviving oocytes in the D-MT and A-MT subgroups and D-0 and A-0 subgroups were cultured in a fertilization media with and without 10−9 MMT for 2.5 h, respectively, followed by intracytoplasmic sperm injection insemination, embryo culture, and transfer. The survival, cleavage, high-quality embryo, clinical pregnancy, ongoing pregnancy, and implantation rates were significantly higher in the D-MT subgroup than in the D-0 subgroup (all P < 0.05). Similarly, the survival, fertilization, high-quality embryo, and high-quality blastocyst rates were significantly higher in the A-MT subgroup than in the A-0 subgroup (all P < 0.05). These findings indicate that MT addition during cryopreservation can enhance the development of vitrified-warmed human oocytes and improve clinical outcomes.
•MT application in cryopreservation can enhance the development of freeze–thawed human oocytes and improve clinical outcomes.•This study suggest that heterogeneity issues do not need to be considered for oocyte donation therapy.•This study offers a promising solution regarding the cryopreservation of human oocytes.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>38734365</pmid><doi>10.1016/j.cryobiol.2024.104902</doi><tpages>1</tpages></addata></record> |
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| subjects | Adult blastocyst Blastocyst - drug effects Clinical outcomes cryobiology Cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology Development embryo culture Embryo Culture Techniques - methods Embryo Transfer Female Fertilization in Vitro - methods freeze-thaw cycles Human oocyte Humans insemination intracytoplasmic sperm injection Melatonin Melatonin - pharmacology oocytes Oocytes - drug effects Pregnancy Pregnancy Rate Sperm Injections, Intracytoplasmic - methods vitrification Vitrification - drug effects |
| title | Melatonin application during cryopreservation improves the development and clinical outcomes of human vitrified–warmed oocytes |
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