In vitro rooting of Quercus robur, activated charcoal vs. exogenous auxin: a morphophysiological approach
In plant cultivation, morphophysiological status of the plantlets is crucial. We aimed to assess how activated charcoal (AC) and synthetic auxins, separately and in combination, affect the biochemistry, anatomy, and physiology of Quercus robur plantlets. Two concurrent experiments were conducted usi...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2024, Vol.156 (1), p.24-24, Article 24 |
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| container_title | Plant cell, tissue and organ culture |
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| creator | Martins, João Paulo Rodrigues Wawrzyniak, Mikołaj Krzysztof Kalemba, Ewa Marzena Ley-López, Juan Manuel Lira, Jean Marcel Sousa Chmielarz, Paweł |
| description | In plant cultivation, morphophysiological status of the plantlets is crucial. We aimed to assess how activated charcoal (AC) and synthetic auxins, separately and in combination, affect the biochemistry, anatomy, and physiology of
Quercus robur
plantlets. Two concurrent experiments were conducted using indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) as exogenous auxins, with or without AC. Plantlets grown without exogenous auxins and AC displayed weak root development, reduced functionality, lower photosynthetic pigments, and elevated starch and free IAA content. Incorporating IAA or IBA into the medium triggered positive morphophysiological changes, such as aerenchyma formation, increased hydrogen peroxide, and reduced free IAA levels in the in vitro-produced clones. Plantlets cultured with 2 g L
−1
AC exhibited higher biomass and more photosynthetic pigments. AC also enhanced the photosynthetic apparatus, as indicated by higher total performance index (PI
(TOTAL)
) values. Importantly, AC improved rooting success and overall morphophysiological condition, regardless of exogenous auxin presence. These findings provide novel insights into in vitro rooting modulation with AC and exogenous auxins, promising advancements in the growth and performance of
Q. robur
plantlets.
Key Message
Exogenous auxin supplementation is not a limiting factor for rhizogenesis. Root functionality was a response to physiological disorders. AC can induce plantlets with higher connectivity of the photosystem II units. |
| doi_str_mv | 10.1007/s11240-023-02656-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153556634</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2901290608</sourcerecordid><originalsourceid>FETCH-LOGICAL-c347t-28699a6e1b961b205bd23bc521aa632ee5af7d393b541870adcf76b74d2fb0b13</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMoWKt_wFPAiwe35mOT7HqT4kehIIKeQ5LNdlO2mzXZLe2_N1pB8OBhGBied-adF4BLjGYYIXEbMSY5yhChqTjjmTgCE8wEzRjK82MwQZiLjBdMnIKzGNcIIU5zPAFu0cGtG4KHwfvBdSvoa_g62mDGmEZ6DDdQmcFt1WAraBoVjFct3MYZtDu_sp1PnBp3rruDCm586BvfN_vofOtXziRU9X3wyjTn4KRWbbQXP30K3h8f3ubP2fLlaTG_X2aG5mLISMHLUnGLdcmxJojpilBtGMFKcUqsZaoWFS2pZjkuBFKVqQXXIq9IrZHGdAquD3vT2Y_RxkFuXDS2bVVnk1lJMaOM8fR-Qq_-oGs_hi65k6REOBVHRaLIgTLBxxhsLfvgNirsJUbyK315SF-m9OV3-lIkET2IYoK7lQ2_q_9RfQJ4Dohs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2901290608</pqid></control><display><type>article</type><title>In vitro rooting of Quercus robur, activated charcoal vs. exogenous auxin: a morphophysiological approach</title><source>SpringerLink Journals</source><creator>Martins, João Paulo Rodrigues ; Wawrzyniak, Mikołaj Krzysztof ; Kalemba, Ewa Marzena ; Ley-López, Juan Manuel ; Lira, Jean Marcel Sousa ; Chmielarz, Paweł</creator><creatorcontrib>Martins, João Paulo Rodrigues ; Wawrzyniak, Mikołaj Krzysztof ; Kalemba, Ewa Marzena ; Ley-López, Juan Manuel ; Lira, Jean Marcel Sousa ; Chmielarz, Paweł</creatorcontrib><description>In plant cultivation, morphophysiological status of the plantlets is crucial. We aimed to assess how activated charcoal (AC) and synthetic auxins, separately and in combination, affect the biochemistry, anatomy, and physiology of
Quercus robur
plantlets. Two concurrent experiments were conducted using indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) as exogenous auxins, with or without AC. Plantlets grown without exogenous auxins and AC displayed weak root development, reduced functionality, lower photosynthetic pigments, and elevated starch and free IAA content. Incorporating IAA or IBA into the medium triggered positive morphophysiological changes, such as aerenchyma formation, increased hydrogen peroxide, and reduced free IAA levels in the in vitro-produced clones. Plantlets cultured with 2 g L
−1
AC exhibited higher biomass and more photosynthetic pigments. AC also enhanced the photosynthetic apparatus, as indicated by higher total performance index (PI
(TOTAL)
) values. Importantly, AC improved rooting success and overall morphophysiological condition, regardless of exogenous auxin presence. These findings provide novel insights into in vitro rooting modulation with AC and exogenous auxins, promising advancements in the growth and performance of
Q. robur
plantlets.
Key Message
Exogenous auxin supplementation is not a limiting factor for rhizogenesis. Root functionality was a response to physiological disorders. AC can induce plantlets with higher connectivity of the photosystem II units.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-023-02656-7</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acetic acid ; Acids ; Activated carbon ; Activated charcoal ; aerenchyma ; Anatomy & physiology ; Auxins ; Biochemistry ; biomass ; Biomedical and Life Sciences ; Butyric acid ; Charcoal ; Flowers & plants ; Hydrogen peroxide ; indole acetic acid ; indole butyric acid ; Indole-3-butyric acid ; Indoleacetic acid ; Life Sciences ; Original Article ; Performance indices ; Photosynthesis ; Photosynthetic apparatus ; Photosynthetic pigments ; Photosystem II ; Physiological effects ; Physiology ; Pigments ; Plant Genetics and Genomics ; Plant growth ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Plantlets ; Quercus robur ; Root development ; Rooting ; Seeds ; starch ; Success</subject><ispartof>Plant cell, tissue and organ culture, 2024, Vol.156 (1), p.24-24, Article 24</ispartof><rights>The Author(s) 2023</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c347t-28699a6e1b961b205bd23bc521aa632ee5af7d393b541870adcf76b74d2fb0b13</cites><orcidid>0000-0003-0554-6793</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-023-02656-7$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-023-02656-7$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Martins, João Paulo Rodrigues</creatorcontrib><creatorcontrib>Wawrzyniak, Mikołaj Krzysztof</creatorcontrib><creatorcontrib>Kalemba, Ewa Marzena</creatorcontrib><creatorcontrib>Ley-López, Juan Manuel</creatorcontrib><creatorcontrib>Lira, Jean Marcel Sousa</creatorcontrib><creatorcontrib>Chmielarz, Paweł</creatorcontrib><title>In vitro rooting of Quercus robur, activated charcoal vs. exogenous auxin: a morphophysiological approach</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>In plant cultivation, morphophysiological status of the plantlets is crucial. We aimed to assess how activated charcoal (AC) and synthetic auxins, separately and in combination, affect the biochemistry, anatomy, and physiology of
Quercus robur
plantlets. Two concurrent experiments were conducted using indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) as exogenous auxins, with or without AC. Plantlets grown without exogenous auxins and AC displayed weak root development, reduced functionality, lower photosynthetic pigments, and elevated starch and free IAA content. Incorporating IAA or IBA into the medium triggered positive morphophysiological changes, such as aerenchyma formation, increased hydrogen peroxide, and reduced free IAA levels in the in vitro-produced clones. Plantlets cultured with 2 g L
−1
AC exhibited higher biomass and more photosynthetic pigments. AC also enhanced the photosynthetic apparatus, as indicated by higher total performance index (PI
(TOTAL)
) values. Importantly, AC improved rooting success and overall morphophysiological condition, regardless of exogenous auxin presence. These findings provide novel insights into in vitro rooting modulation with AC and exogenous auxins, promising advancements in the growth and performance of
Q. robur
plantlets.
Key Message
Exogenous auxin supplementation is not a limiting factor for rhizogenesis. Root functionality was a response to physiological disorders. AC can induce plantlets with higher connectivity of the photosystem II units.</description><subject>Acetic acid</subject><subject>Acids</subject><subject>Activated carbon</subject><subject>Activated charcoal</subject><subject>aerenchyma</subject><subject>Anatomy & physiology</subject><subject>Auxins</subject><subject>Biochemistry</subject><subject>biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Butyric acid</subject><subject>Charcoal</subject><subject>Flowers & plants</subject><subject>Hydrogen peroxide</subject><subject>indole acetic acid</subject><subject>indole butyric acid</subject><subject>Indole-3-butyric acid</subject><subject>Indoleacetic acid</subject><subject>Life Sciences</subject><subject>Original Article</subject><subject>Performance indices</subject><subject>Photosynthesis</subject><subject>Photosynthetic apparatus</subject><subject>Photosynthetic pigments</subject><subject>Photosystem II</subject><subject>Physiological effects</subject><subject>Physiology</subject><subject>Pigments</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plantlets</subject><subject>Quercus robur</subject><subject>Root development</subject><subject>Rooting</subject><subject>Seeds</subject><subject>starch</subject><subject>Success</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1LAzEQhoMoWKt_wFPAiwe35mOT7HqT4kehIIKeQ5LNdlO2mzXZLe2_N1pB8OBhGBied-adF4BLjGYYIXEbMSY5yhChqTjjmTgCE8wEzRjK82MwQZiLjBdMnIKzGNcIIU5zPAFu0cGtG4KHwfvBdSvoa_g62mDGmEZ6DDdQmcFt1WAraBoVjFct3MYZtDu_sp1PnBp3rruDCm586BvfN_vofOtXziRU9X3wyjTn4KRWbbQXP30K3h8f3ubP2fLlaTG_X2aG5mLISMHLUnGLdcmxJojpilBtGMFKcUqsZaoWFS2pZjkuBFKVqQXXIq9IrZHGdAquD3vT2Y_RxkFuXDS2bVVnk1lJMaOM8fR-Qq_-oGs_hi65k6REOBVHRaLIgTLBxxhsLfvgNirsJUbyK315SF-m9OV3-lIkET2IYoK7lQ2_q_9RfQJ4Dohs</recordid><startdate>2024</startdate><enddate>2024</enddate><creator>Martins, João Paulo Rodrigues</creator><creator>Wawrzyniak, Mikołaj Krzysztof</creator><creator>Kalemba, Ewa Marzena</creator><creator>Ley-López, Juan Manuel</creator><creator>Lira, Jean Marcel Sousa</creator><creator>Chmielarz, Paweł</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-0554-6793</orcidid></search><sort><creationdate>2024</creationdate><title>In vitro rooting of Quercus robur, activated charcoal vs. exogenous auxin: a morphophysiological approach</title><author>Martins, João Paulo Rodrigues ; Wawrzyniak, Mikołaj Krzysztof ; Kalemba, Ewa Marzena ; Ley-López, Juan Manuel ; Lira, Jean Marcel Sousa ; Chmielarz, Paweł</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-28699a6e1b961b205bd23bc521aa632ee5af7d393b541870adcf76b74d2fb0b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetic acid</topic><topic>Acids</topic><topic>Activated carbon</topic><topic>Activated charcoal</topic><topic>aerenchyma</topic><topic>Anatomy & physiology</topic><topic>Auxins</topic><topic>Biochemistry</topic><topic>biomass</topic><topic>Biomedical and Life Sciences</topic><topic>Butyric acid</topic><topic>Charcoal</topic><topic>Flowers & plants</topic><topic>Hydrogen peroxide</topic><topic>indole acetic acid</topic><topic>indole butyric acid</topic><topic>Indole-3-butyric acid</topic><topic>Indoleacetic acid</topic><topic>Life Sciences</topic><topic>Original Article</topic><topic>Performance indices</topic><topic>Photosynthesis</topic><topic>Photosynthetic apparatus</topic><topic>Photosynthetic pigments</topic><topic>Photosystem II</topic><topic>Physiological effects</topic><topic>Physiology</topic><topic>Pigments</topic><topic>Plant Genetics and Genomics</topic><topic>Plant growth</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plantlets</topic><topic>Quercus robur</topic><topic>Root development</topic><topic>Rooting</topic><topic>Seeds</topic><topic>starch</topic><topic>Success</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martins, João Paulo Rodrigues</creatorcontrib><creatorcontrib>Wawrzyniak, Mikołaj Krzysztof</creatorcontrib><creatorcontrib>Kalemba, Ewa Marzena</creatorcontrib><creatorcontrib>Ley-López, Juan Manuel</creatorcontrib><creatorcontrib>Lira, Jean Marcel Sousa</creatorcontrib><creatorcontrib>Chmielarz, Paweł</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martins, João Paulo Rodrigues</au><au>Wawrzyniak, Mikołaj Krzysztof</au><au>Kalemba, Ewa Marzena</au><au>Ley-López, Juan Manuel</au><au>Lira, Jean Marcel Sousa</au><au>Chmielarz, Paweł</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro rooting of Quercus robur, activated charcoal vs. exogenous auxin: a morphophysiological approach</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2024</date><risdate>2024</risdate><volume>156</volume><issue>1</issue><spage>24</spage><epage>24</epage><pages>24-24</pages><artnum>24</artnum><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>In plant cultivation, morphophysiological status of the plantlets is crucial. We aimed to assess how activated charcoal (AC) and synthetic auxins, separately and in combination, affect the biochemistry, anatomy, and physiology of
Quercus robur
plantlets. Two concurrent experiments were conducted using indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) as exogenous auxins, with or without AC. Plantlets grown without exogenous auxins and AC displayed weak root development, reduced functionality, lower photosynthetic pigments, and elevated starch and free IAA content. Incorporating IAA or IBA into the medium triggered positive morphophysiological changes, such as aerenchyma formation, increased hydrogen peroxide, and reduced free IAA levels in the in vitro-produced clones. Plantlets cultured with 2 g L
−1
AC exhibited higher biomass and more photosynthetic pigments. AC also enhanced the photosynthetic apparatus, as indicated by higher total performance index (PI
(TOTAL)
) values. Importantly, AC improved rooting success and overall morphophysiological condition, regardless of exogenous auxin presence. These findings provide novel insights into in vitro rooting modulation with AC and exogenous auxins, promising advancements in the growth and performance of
Q. robur
plantlets.
Key Message
Exogenous auxin supplementation is not a limiting factor for rhizogenesis. Root functionality was a response to physiological disorders. AC can induce plantlets with higher connectivity of the photosystem II units.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-023-02656-7</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-0554-6793</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant cell, tissue and organ culture, 2024, Vol.156 (1), p.24-24, Article 24 |
| issn | 0167-6857 1573-5044 |
| language | eng |
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| subjects | Acetic acid Acids Activated carbon Activated charcoal aerenchyma Anatomy & physiology Auxins Biochemistry biomass Biomedical and Life Sciences Butyric acid Charcoal Flowers & plants Hydrogen peroxide indole acetic acid indole butyric acid Indole-3-butyric acid Indoleacetic acid Life Sciences Original Article Performance indices Photosynthesis Photosynthetic apparatus Photosynthetic pigments Photosystem II Physiological effects Physiology Pigments Plant Genetics and Genomics Plant growth Plant Pathology Plant Physiology Plant Sciences Plantlets Quercus robur Root development Rooting Seeds starch Success |
| title | In vitro rooting of Quercus robur, activated charcoal vs. exogenous auxin: a morphophysiological approach |
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