Establishment of Parentage Identification Method for Sea Urchin Strongylocentrotus intermedius Based on SSR-seq Technology
To establish a parentage identification method for , 15 microsatellite loci and simple sequence repeat sequencing (SSR-seq) technology were used to perform SSR sequencing and typing of the validation population with known pedigree information and the simulation population. Cervus v3.0 was used for g...
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| Veröffentlicht in: | Genes 2024-05, Vol.15 (5), p.630 |
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| container_title | Genes |
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| creator | Jiang, Xuechun Liu, Lei Guo, Hao Liu, Peng Tian, Wenzhuo Ou, Fanjiang Ding, Jun Zhang, Weijie Chang, Yaqing |
| description | To establish a parentage identification method for
, 15 microsatellite loci and simple sequence repeat sequencing (SSR-seq) technology were used to perform SSR sequencing and typing of the validation population with known pedigree information and the simulation population. Cervus v3.0 was used for gene frequency statistics, simulated analysis, and parentage identification analysis. The results showed that, in validation population, using 15 microsatellite loci, the highest success rate of parent pairs identification was 86%, the highest success rate of female parent identification was 93%, and the highest success rate of male parent identification was 90%. The simulated population was analyzed using 12-15 loci, and the identification rate was up to 90%. In cases where accurate parentage was not achieved, individuals could exhibit genetic similarities with 1-3 male or female parents. Individuals identified as lacking a genetic relationship can be selected as parents to prevent inbreeding. This study shows that parent pairs or single parents of most offspring can be identified successfully using these 15 selected loci. The results lay a foundation for the establishment of a parentage identification method for
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| doi_str_mv | 10.3390/genes15050630 |
| format | Article |
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, 15 microsatellite loci and simple sequence repeat sequencing (SSR-seq) technology were used to perform SSR sequencing and typing of the validation population with known pedigree information and the simulation population. Cervus v3.0 was used for gene frequency statistics, simulated analysis, and parentage identification analysis. The results showed that, in validation population, using 15 microsatellite loci, the highest success rate of parent pairs identification was 86%, the highest success rate of female parent identification was 93%, and the highest success rate of male parent identification was 90%. The simulated population was analyzed using 12-15 loci, and the identification rate was up to 90%. In cases where accurate parentage was not achieved, individuals could exhibit genetic similarities with 1-3 male or female parents. Individuals identified as lacking a genetic relationship can be selected as parents to prevent inbreeding. This study shows that parent pairs or single parents of most offspring can be identified successfully using these 15 selected loci. The results lay a foundation for the establishment of a parentage identification method for
.</description><identifier>ISSN: 2073-4425</identifier><identifier>EISSN: 2073-4425</identifier><identifier>DOI: 10.3390/genes15050630</identifier><identifier>PMID: 38790259</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Accuracy ; Animals ; Annealing ; Bioengineering ; Capillary electrophoresis ; Cervus ; Eggs ; Female ; Females ; Gene frequency ; Gene Frequency - genetics ; Genetic relationship ; Genetic testing ; Identification systems ; Inbreeding ; Male ; males ; microsatellite repeats ; Microsatellite Repeats - genetics ; parentage ; Paternity ; Pedigree ; progeny ; Sequence Analysis, DNA - methods ; Sperm ; Statistical analysis ; statistics ; Strongylocentrotus - genetics ; Strongylocentrotus intermedius ; Temperature</subject><ispartof>Genes, 2024-05, Vol.15 (5), p.630</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c349t-35da8b0c2f4c4f8f058b847d4d7ab50d724aa90e1b72a26a778151be396306483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38790259$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Xuechun</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Guo, Hao</creatorcontrib><creatorcontrib>Liu, Peng</creatorcontrib><creatorcontrib>Tian, Wenzhuo</creatorcontrib><creatorcontrib>Ou, Fanjiang</creatorcontrib><creatorcontrib>Ding, Jun</creatorcontrib><creatorcontrib>Zhang, Weijie</creatorcontrib><creatorcontrib>Chang, Yaqing</creatorcontrib><title>Establishment of Parentage Identification Method for Sea Urchin Strongylocentrotus intermedius Based on SSR-seq Technology</title><title>Genes</title><addtitle>Genes (Basel)</addtitle><description>To establish a parentage identification method for
, 15 microsatellite loci and simple sequence repeat sequencing (SSR-seq) technology were used to perform SSR sequencing and typing of the validation population with known pedigree information and the simulation population. Cervus v3.0 was used for gene frequency statistics, simulated analysis, and parentage identification analysis. The results showed that, in validation population, using 15 microsatellite loci, the highest success rate of parent pairs identification was 86%, the highest success rate of female parent identification was 93%, and the highest success rate of male parent identification was 90%. The simulated population was analyzed using 12-15 loci, and the identification rate was up to 90%. In cases where accurate parentage was not achieved, individuals could exhibit genetic similarities with 1-3 male or female parents. Individuals identified as lacking a genetic relationship can be selected as parents to prevent inbreeding. This study shows that parent pairs or single parents of most offspring can be identified successfully using these 15 selected loci. The results lay a foundation for the establishment of a parentage identification method for
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, 15 microsatellite loci and simple sequence repeat sequencing (SSR-seq) technology were used to perform SSR sequencing and typing of the validation population with known pedigree information and the simulation population. Cervus v3.0 was used for gene frequency statistics, simulated analysis, and parentage identification analysis. The results showed that, in validation population, using 15 microsatellite loci, the highest success rate of parent pairs identification was 86%, the highest success rate of female parent identification was 93%, and the highest success rate of male parent identification was 90%. The simulated population was analyzed using 12-15 loci, and the identification rate was up to 90%. In cases where accurate parentage was not achieved, individuals could exhibit genetic similarities with 1-3 male or female parents. Individuals identified as lacking a genetic relationship can be selected as parents to prevent inbreeding. This study shows that parent pairs or single parents of most offspring can be identified successfully using these 15 selected loci. The results lay a foundation for the establishment of a parentage identification method for
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| subjects | Accuracy Animals Annealing Bioengineering Capillary electrophoresis Cervus Eggs Female Females Gene frequency Gene Frequency - genetics Genetic relationship Genetic testing Identification systems Inbreeding Male males microsatellite repeats Microsatellite Repeats - genetics parentage Paternity Pedigree progeny Sequence Analysis, DNA - methods Sperm Statistical analysis statistics Strongylocentrotus - genetics Strongylocentrotus intermedius Temperature |
| title | Establishment of Parentage Identification Method for Sea Urchin Strongylocentrotus intermedius Based on SSR-seq Technology |
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