Mixotrophic culture of Chaetoceros sp. and the synergistic carbon and energy metabolism
[Display omitted] •Optimum carbon source and concentration for mixotrophic Chaetoceros sp. are screened.•Intermediate metabolites inhibition regulates the reduction in CBB cycle.•The different reactions in mixotrophic Chaetoceros is due to carbon and energy metabolism. This research studied the meta...
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| Veröffentlicht in: | Bioresource technology 2023-12, Vol.390, p.129912, Article 129912 |
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| creator | Shan, Shengzhou Wang, Shanshan Yan, Xi Chen, Kang Liang, Li Li, Xiaohui Zhou, Chengxu Yan, Xiaojun Ruan, Roger Cheng, Pengfei |
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•Optimum carbon source and concentration for mixotrophic Chaetoceros sp. are screened.•Intermediate metabolites inhibition regulates the reduction in CBB cycle.•The different reactions in mixotrophic Chaetoceros is due to carbon and energy metabolism.
This research studied the metabolic mechanism of the mixotrophic Chaetoceros sp. The results showed this alga had the highest cell density and growth rate of 47.72 × 105 cells mL−1 and 0.41 d-1, respectively, with a maximum dry weight of 2.90 g/L, when compared to photoautotrophic and photoheterotrophic modes. Compared to photoheterotrophy, transcriptomics results showed the Rubisco, PGK, and GAPDH related genes were separately up-regulated by 1.03, 2.36, and 1.36 times in CBB cycle in mixotrophic mode, suggesting intermediate metabolites of EMP and PPP can enter the chloroplast via transporter proteins, or membrane permeation, and feedback inhibition regulates the reduction of multiple reactions in CBB cycle. Chaetoceros sp. achieves high biomass by utilizing ATP and carbon structures from EMP and PPP pathways, and the addition of NaHCO3 leads to an up-regulation of CBB cycle for the mixotrophic alga, resulting in higher biomass compared to the photoheterotrophic mode. |
| doi_str_mv | 10.1016/j.biortech.2023.129912 |
| format | Article |
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•Optimum carbon source and concentration for mixotrophic Chaetoceros sp. are screened.•Intermediate metabolites inhibition regulates the reduction in CBB cycle.•The different reactions in mixotrophic Chaetoceros is due to carbon and energy metabolism.
This research studied the metabolic mechanism of the mixotrophic Chaetoceros sp. The results showed this alga had the highest cell density and growth rate of 47.72 × 105 cells mL−1 and 0.41 d-1, respectively, with a maximum dry weight of 2.90 g/L, when compared to photoautotrophic and photoheterotrophic modes. Compared to photoheterotrophy, transcriptomics results showed the Rubisco, PGK, and GAPDH related genes were separately up-regulated by 1.03, 2.36, and 1.36 times in CBB cycle in mixotrophic mode, suggesting intermediate metabolites of EMP and PPP can enter the chloroplast via transporter proteins, or membrane permeation, and feedback inhibition regulates the reduction of multiple reactions in CBB cycle. Chaetoceros sp. achieves high biomass by utilizing ATP and carbon structures from EMP and PPP pathways, and the addition of NaHCO3 leads to an up-regulation of CBB cycle for the mixotrophic alga, resulting in higher biomass compared to the photoheterotrophic mode.</description><identifier>ISSN: 0960-8524</identifier><identifier>ISSN: 1873-2976</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2023.129912</identifier><identifier>PMID: 37879446</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>algae ; Biomass ; carbon ; Carbon - metabolism ; Chaetoceros ; Chaetoceros sp ; chloroplasts ; culture media ; Energy Metabolism ; Gene Expression Profiling ; metabolites ; Mixotrophic culture ; permeability ; photoheterotrophs ; ribulose-bisphosphate carboxylase ; Synergistic mechanism ; technology</subject><ispartof>Bioresource technology, 2023-12, Vol.390, p.129912, Article 129912</ispartof><rights>2023 Elsevier Ltd</rights><rights>Copyright © 2023 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-f8976bb11bf2cc9dc74fb298b46893b92c3d475ca416712405a531b31a252a963</citedby><cites>FETCH-LOGICAL-c401t-f8976bb11bf2cc9dc74fb298b46893b92c3d475ca416712405a531b31a252a963</cites><orcidid>0000-0001-8835-2649</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37879446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shan, Shengzhou</creatorcontrib><creatorcontrib>Wang, Shanshan</creatorcontrib><creatorcontrib>Yan, Xi</creatorcontrib><creatorcontrib>Chen, Kang</creatorcontrib><creatorcontrib>Liang, Li</creatorcontrib><creatorcontrib>Li, Xiaohui</creatorcontrib><creatorcontrib>Zhou, Chengxu</creatorcontrib><creatorcontrib>Yan, Xiaojun</creatorcontrib><creatorcontrib>Ruan, Roger</creatorcontrib><creatorcontrib>Cheng, Pengfei</creatorcontrib><title>Mixotrophic culture of Chaetoceros sp. and the synergistic carbon and energy metabolism</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>[Display omitted]
•Optimum carbon source and concentration for mixotrophic Chaetoceros sp. are screened.•Intermediate metabolites inhibition regulates the reduction in CBB cycle.•The different reactions in mixotrophic Chaetoceros is due to carbon and energy metabolism.
This research studied the metabolic mechanism of the mixotrophic Chaetoceros sp. The results showed this alga had the highest cell density and growth rate of 47.72 × 105 cells mL−1 and 0.41 d-1, respectively, with a maximum dry weight of 2.90 g/L, when compared to photoautotrophic and photoheterotrophic modes. Compared to photoheterotrophy, transcriptomics results showed the Rubisco, PGK, and GAPDH related genes were separately up-regulated by 1.03, 2.36, and 1.36 times in CBB cycle in mixotrophic mode, suggesting intermediate metabolites of EMP and PPP can enter the chloroplast via transporter proteins, or membrane permeation, and feedback inhibition regulates the reduction of multiple reactions in CBB cycle. Chaetoceros sp. achieves high biomass by utilizing ATP and carbon structures from EMP and PPP pathways, and the addition of NaHCO3 leads to an up-regulation of CBB cycle for the mixotrophic alga, resulting in higher biomass compared to the photoheterotrophic mode.</description><subject>algae</subject><subject>Biomass</subject><subject>carbon</subject><subject>Carbon - metabolism</subject><subject>Chaetoceros</subject><subject>Chaetoceros sp</subject><subject>chloroplasts</subject><subject>culture media</subject><subject>Energy Metabolism</subject><subject>Gene Expression Profiling</subject><subject>metabolites</subject><subject>Mixotrophic culture</subject><subject>permeability</subject><subject>photoheterotrophs</subject><subject>ribulose-bisphosphate carboxylase</subject><subject>Synergistic mechanism</subject><subject>technology</subject><issn>0960-8524</issn><issn>1873-2976</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQQC0EotuWv1DlyCXBHjuxfQOtoEUq4gLq0bKdCetVEi-2g7r_nizbcu1ppJk3X4-QG0YbRln3Yd-4EFNBv2uAAm8YaM3gFdkwJXkNWnavyYbqjtaqBXFBLnPeU0o5k_CWXHCppBai25CHb-ExlhQPu-Arv4xlSVjFodruLJboMcVc5UNT2bmvyg6rfJwx_Qq5nHCbXJz_lfCUPVYTFuviGPJ0Td4Mdsz47ilekZ9fPv_Y3tX332-_bj_d115QVupBrZc6x5gbwHvdeykGB1o50SnNnQbPeyFbbwXrJANBW9ty5jiz0ILVHb8i789zDyn-XjAXM4XscRztjHHJhrOWA1Udky-ioBRwAAFsRbsz6tf_c8LBHFKYbDoaRs3Jv9mbZ__m5N-c_a-NN087Fjdh_7_tWfgKfDwDuEr5EzCZ7APOHvuQ0BfTx_DSjr81-ZmP</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Shan, Shengzhou</creator><creator>Wang, Shanshan</creator><creator>Yan, Xi</creator><creator>Chen, Kang</creator><creator>Liang, Li</creator><creator>Li, Xiaohui</creator><creator>Zhou, Chengxu</creator><creator>Yan, Xiaojun</creator><creator>Ruan, Roger</creator><creator>Cheng, Pengfei</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-8835-2649</orcidid></search><sort><creationdate>202312</creationdate><title>Mixotrophic culture of Chaetoceros sp. and the synergistic carbon and energy metabolism</title><author>Shan, Shengzhou ; Wang, Shanshan ; Yan, Xi ; Chen, Kang ; Liang, Li ; Li, Xiaohui ; Zhou, Chengxu ; Yan, Xiaojun ; Ruan, Roger ; Cheng, Pengfei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-f8976bb11bf2cc9dc74fb298b46893b92c3d475ca416712405a531b31a252a963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>algae</topic><topic>Biomass</topic><topic>carbon</topic><topic>Carbon - metabolism</topic><topic>Chaetoceros</topic><topic>Chaetoceros sp</topic><topic>chloroplasts</topic><topic>culture media</topic><topic>Energy Metabolism</topic><topic>Gene Expression Profiling</topic><topic>metabolites</topic><topic>Mixotrophic culture</topic><topic>permeability</topic><topic>photoheterotrophs</topic><topic>ribulose-bisphosphate carboxylase</topic><topic>Synergistic mechanism</topic><topic>technology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shan, Shengzhou</creatorcontrib><creatorcontrib>Wang, Shanshan</creatorcontrib><creatorcontrib>Yan, Xi</creatorcontrib><creatorcontrib>Chen, Kang</creatorcontrib><creatorcontrib>Liang, Li</creatorcontrib><creatorcontrib>Li, Xiaohui</creatorcontrib><creatorcontrib>Zhou, Chengxu</creatorcontrib><creatorcontrib>Yan, Xiaojun</creatorcontrib><creatorcontrib>Ruan, Roger</creatorcontrib><creatorcontrib>Cheng, Pengfei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shan, Shengzhou</au><au>Wang, Shanshan</au><au>Yan, Xi</au><au>Chen, Kang</au><au>Liang, Li</au><au>Li, Xiaohui</au><au>Zhou, Chengxu</au><au>Yan, Xiaojun</au><au>Ruan, Roger</au><au>Cheng, Pengfei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mixotrophic culture of Chaetoceros sp. and the synergistic carbon and energy metabolism</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2023-12</date><risdate>2023</risdate><volume>390</volume><spage>129912</spage><pages>129912-</pages><artnum>129912</artnum><issn>0960-8524</issn><issn>1873-2976</issn><eissn>1873-2976</eissn><abstract>[Display omitted]
•Optimum carbon source and concentration for mixotrophic Chaetoceros sp. are screened.•Intermediate metabolites inhibition regulates the reduction in CBB cycle.•The different reactions in mixotrophic Chaetoceros is due to carbon and energy metabolism.
This research studied the metabolic mechanism of the mixotrophic Chaetoceros sp. The results showed this alga had the highest cell density and growth rate of 47.72 × 105 cells mL−1 and 0.41 d-1, respectively, with a maximum dry weight of 2.90 g/L, when compared to photoautotrophic and photoheterotrophic modes. Compared to photoheterotrophy, transcriptomics results showed the Rubisco, PGK, and GAPDH related genes were separately up-regulated by 1.03, 2.36, and 1.36 times in CBB cycle in mixotrophic mode, suggesting intermediate metabolites of EMP and PPP can enter the chloroplast via transporter proteins, or membrane permeation, and feedback inhibition regulates the reduction of multiple reactions in CBB cycle. Chaetoceros sp. achieves high biomass by utilizing ATP and carbon structures from EMP and PPP pathways, and the addition of NaHCO3 leads to an up-regulation of CBB cycle for the mixotrophic alga, resulting in higher biomass compared to the photoheterotrophic mode.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>37879446</pmid><doi>10.1016/j.biortech.2023.129912</doi><orcidid>https://orcid.org/0000-0001-8835-2649</orcidid></addata></record> |
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| subjects | algae Biomass carbon Carbon - metabolism Chaetoceros Chaetoceros sp chloroplasts culture media Energy Metabolism Gene Expression Profiling metabolites Mixotrophic culture permeability photoheterotrophs ribulose-bisphosphate carboxylase Synergistic mechanism technology |
| title | Mixotrophic culture of Chaetoceros sp. and the synergistic carbon and energy metabolism |
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