Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil-Borne Chickpea Fungal Pathogens
Defensins are broad-spectrum antimicrobial peptides that play an important role in providing innate immunity to various biotic stresses in plants. We identified and characterized 22 defensin (DEF) and defensin-like (DEFL) genes in chickpea ( Cicer arientinum ) based on their structures, expression,...
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| Veröffentlicht in: | Journal of plant growth regulation 2023-10, Vol.42 (10), p.6244-6260 |
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| creator | Nitnavare, Rahul B. Pothana, Arunima Yeshvekar, Richa K. Bhattacharya, Joorie Sapara, Vidhi Reddy, Palakolanu Sudhakar Ramtirtha, Yogendra Tarafdar, Avijit Sharma, Mamta Bhatnagar-Mathur, Pooja |
| description | Defensins are broad-spectrum antimicrobial peptides that play an important role in providing innate immunity to various biotic stresses in plants. We identified and characterized 22 defensin (DEF) and defensin-like (DEFL) genes in chickpea (
Cicer arientinum
) based on their structures, expression, chromosomal localization, conserved motifs, and
cis
-regulatory elements. The localization of
DEF
and
DEFL
genes in chickpea genome revealed the presence of at least two clusters that are likely evolved through local gene duplications. Genotype-specific responses of several
CaDEF
and
CaDEFL
genes in fungal bioassays suggested their involvement in defense against fungal pathogens such as hemi-biotrophic
F. oxysporum
f. sp.
ciceris
and dry root rot causing necrotrophic
R. bataticola
. Molecular docking studies revealed interactions of CaDEFs with fungal plasma membrane components such as phosphatidylserine (PS) and glucosylceramide (GluCer) and their binding sites were identified. Our data will be useful to identify potential candidate genes and their role in host-plant resistance in chickpea, besides presenting opportunities for their potential for possible deployment in other crops. |
| doi_str_mv | 10.1007/s00344-022-10811-1 |
| format | Article |
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Cicer arientinum
) based on their structures, expression, chromosomal localization, conserved motifs, and
cis
-regulatory elements. The localization of
DEF
and
DEFL
genes in chickpea genome revealed the presence of at least two clusters that are likely evolved through local gene duplications. Genotype-specific responses of several
CaDEF
and
CaDEFL
genes in fungal bioassays suggested their involvement in defense against fungal pathogens such as hemi-biotrophic
F. oxysporum
f. sp.
ciceris
and dry root rot causing necrotrophic
R. bataticola
. Molecular docking studies revealed interactions of CaDEFs with fungal plasma membrane components such as phosphatidylserine (PS) and glucosylceramide (GluCer) and their binding sites were identified. Our data will be useful to identify potential candidate genes and their role in host-plant resistance in chickpea, besides presenting opportunities for their potential for possible deployment in other crops.</description><identifier>ISSN: 0721-7595</identifier><identifier>EISSN: 1435-8107</identifier><identifier>DOI: 10.1007/s00344-022-10811-1</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Agriculture ; Antimicrobial peptides ; Binding sites ; Bioassays ; Biomedical and Life Sciences ; Chickpeas ; chromosome mapping ; Cicer arietinum ; Defensins ; Dry rot ; Fungi ; Genes ; Genomes ; Genotypes ; glucosylceramides ; Host plants ; Innate immunity ; Legumes ; Life Sciences ; Localization ; Molecular docking ; Pathogens ; Peptides ; Phosphatidylserine ; phosphatidylserines ; Plant Anatomy/Development ; plant growth ; Plant Physiology ; Plant resistance ; Plant Sciences ; plasma membrane ; Regulatory sequences ; Root rot ; Soil resistance</subject><ispartof>Journal of plant growth regulation, 2023-10, Vol.42 (10), p.6244-6260</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c303t-8721124610d3e543ac9c111ed5a9bb3f40c948865be169a0c2fc9e6dbd2377d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00344-022-10811-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00344-022-10811-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Nitnavare, Rahul B.</creatorcontrib><creatorcontrib>Pothana, Arunima</creatorcontrib><creatorcontrib>Yeshvekar, Richa K.</creatorcontrib><creatorcontrib>Bhattacharya, Joorie</creatorcontrib><creatorcontrib>Sapara, Vidhi</creatorcontrib><creatorcontrib>Reddy, Palakolanu Sudhakar</creatorcontrib><creatorcontrib>Ramtirtha, Yogendra</creatorcontrib><creatorcontrib>Tarafdar, Avijit</creatorcontrib><creatorcontrib>Sharma, Mamta</creatorcontrib><creatorcontrib>Bhatnagar-Mathur, Pooja</creatorcontrib><title>Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil-Borne Chickpea Fungal Pathogens</title><title>Journal of plant growth regulation</title><addtitle>J Plant Growth Regul</addtitle><description>Defensins are broad-spectrum antimicrobial peptides that play an important role in providing innate immunity to various biotic stresses in plants. We identified and characterized 22 defensin (DEF) and defensin-like (DEFL) genes in chickpea (
Cicer arientinum
) based on their structures, expression, chromosomal localization, conserved motifs, and
cis
-regulatory elements. The localization of
DEF
and
DEFL
genes in chickpea genome revealed the presence of at least two clusters that are likely evolved through local gene duplications. Genotype-specific responses of several
CaDEF
and
CaDEFL
genes in fungal bioassays suggested their involvement in defense against fungal pathogens such as hemi-biotrophic
F. oxysporum
f. sp.
ciceris
and dry root rot causing necrotrophic
R. bataticola
. Molecular docking studies revealed interactions of CaDEFs with fungal plasma membrane components such as phosphatidylserine (PS) and glucosylceramide (GluCer) and their binding sites were identified. Our data will be useful to identify potential candidate genes and their role in host-plant resistance in chickpea, besides presenting opportunities for their potential for possible deployment in other crops.</description><subject>Agriculture</subject><subject>Antimicrobial peptides</subject><subject>Binding sites</subject><subject>Bioassays</subject><subject>Biomedical and Life Sciences</subject><subject>Chickpeas</subject><subject>chromosome mapping</subject><subject>Cicer arietinum</subject><subject>Defensins</subject><subject>Dry rot</subject><subject>Fungi</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genotypes</subject><subject>glucosylceramides</subject><subject>Host plants</subject><subject>Innate immunity</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Molecular docking</subject><subject>Pathogens</subject><subject>Peptides</subject><subject>Phosphatidylserine</subject><subject>phosphatidylserines</subject><subject>Plant Anatomy/Development</subject><subject>plant growth</subject><subject>Plant Physiology</subject><subject>Plant resistance</subject><subject>Plant Sciences</subject><subject>plasma membrane</subject><subject>Regulatory sequences</subject><subject>Root rot</subject><subject>Soil resistance</subject><issn>0721-7595</issn><issn>1435-8107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kcFu1DAQhi0EEkvhBThZ4sLFZcZ2EodbWdgWqRIVLWfL60xSl6y92NlD374ui6jEgdPIo-__NNbP2FuEUwToPhQApbUAKQWCQRT4jK1Qq0YYhO45W0EnUXRN37xkr0q5A8D66FYsrW-D_7knxz_TSLGEyM8pEt-4XZjvP_KrnHahbie-dnEIg1uo8DFl_p1KKIuLnvjZ5EIsC79OYRafUq7xv9bNIU5u5lduuU1T9b9mL0Y3F3rzZ56wH5svN-sLcfnt_Ov67FJ4BWoRpp6LUrcIg6JGK-d7j4g0NK7fbtWowffamLbZEra9Ay9H31M7bAepum4w6oS9P3r3Of06UFls_YaneXaR0qFYhY1CoxVgRd_9g96lQ471OitN2ysjUbeVkkfK51RKptHuc9i5fG8R7GMH9tiBrR3Y3x3YR7U6hkqF40T5Sf2f1AMgcYje</recordid><startdate>20231001</startdate><enddate>20231001</enddate><creator>Nitnavare, Rahul B.</creator><creator>Pothana, Arunima</creator><creator>Yeshvekar, Richa K.</creator><creator>Bhattacharya, Joorie</creator><creator>Sapara, Vidhi</creator><creator>Reddy, Palakolanu Sudhakar</creator><creator>Ramtirtha, Yogendra</creator><creator>Tarafdar, Avijit</creator><creator>Sharma, Mamta</creator><creator>Bhatnagar-Mathur, Pooja</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20231001</creationdate><title>Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil-Borne Chickpea Fungal Pathogens</title><author>Nitnavare, Rahul B. ; Pothana, Arunima ; Yeshvekar, Richa K. ; Bhattacharya, Joorie ; Sapara, Vidhi ; Reddy, Palakolanu Sudhakar ; Ramtirtha, Yogendra ; Tarafdar, Avijit ; Sharma, Mamta ; Bhatnagar-Mathur, Pooja</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-8721124610d3e543ac9c111ed5a9bb3f40c948865be169a0c2fc9e6dbd2377d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Agriculture</topic><topic>Antimicrobial peptides</topic><topic>Binding sites</topic><topic>Bioassays</topic><topic>Biomedical and Life Sciences</topic><topic>Chickpeas</topic><topic>chromosome mapping</topic><topic>Cicer arietinum</topic><topic>Defensins</topic><topic>Dry rot</topic><topic>Fungi</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genotypes</topic><topic>glucosylceramides</topic><topic>Host plants</topic><topic>Innate immunity</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Localization</topic><topic>Molecular docking</topic><topic>Pathogens</topic><topic>Peptides</topic><topic>Phosphatidylserine</topic><topic>phosphatidylserines</topic><topic>Plant Anatomy/Development</topic><topic>plant growth</topic><topic>Plant Physiology</topic><topic>Plant resistance</topic><topic>Plant Sciences</topic><topic>plasma membrane</topic><topic>Regulatory sequences</topic><topic>Root rot</topic><topic>Soil resistance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nitnavare, Rahul B.</creatorcontrib><creatorcontrib>Pothana, Arunima</creatorcontrib><creatorcontrib>Yeshvekar, Richa K.</creatorcontrib><creatorcontrib>Bhattacharya, Joorie</creatorcontrib><creatorcontrib>Sapara, Vidhi</creatorcontrib><creatorcontrib>Reddy, Palakolanu Sudhakar</creatorcontrib><creatorcontrib>Ramtirtha, Yogendra</creatorcontrib><creatorcontrib>Tarafdar, Avijit</creatorcontrib><creatorcontrib>Sharma, Mamta</creatorcontrib><creatorcontrib>Bhatnagar-Mathur, Pooja</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of plant growth regulation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nitnavare, Rahul B.</au><au>Pothana, Arunima</au><au>Yeshvekar, Richa K.</au><au>Bhattacharya, Joorie</au><au>Sapara, Vidhi</au><au>Reddy, Palakolanu Sudhakar</au><au>Ramtirtha, Yogendra</au><au>Tarafdar, Avijit</au><au>Sharma, Mamta</au><au>Bhatnagar-Mathur, Pooja</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil-Borne Chickpea Fungal Pathogens</atitle><jtitle>Journal of plant growth regulation</jtitle><stitle>J Plant Growth Regul</stitle><date>2023-10-01</date><risdate>2023</risdate><volume>42</volume><issue>10</issue><spage>6244</spage><epage>6260</epage><pages>6244-6260</pages><issn>0721-7595</issn><eissn>1435-8107</eissn><abstract>Defensins are broad-spectrum antimicrobial peptides that play an important role in providing innate immunity to various biotic stresses in plants. We identified and characterized 22 defensin (DEF) and defensin-like (DEFL) genes in chickpea (
Cicer arientinum
) based on their structures, expression, chromosomal localization, conserved motifs, and
cis
-regulatory elements. The localization of
DEF
and
DEFL
genes in chickpea genome revealed the presence of at least two clusters that are likely evolved through local gene duplications. Genotype-specific responses of several
CaDEF
and
CaDEFL
genes in fungal bioassays suggested their involvement in defense against fungal pathogens such as hemi-biotrophic
F. oxysporum
f. sp.
ciceris
and dry root rot causing necrotrophic
R. bataticola
. Molecular docking studies revealed interactions of CaDEFs with fungal plasma membrane components such as phosphatidylserine (PS) and glucosylceramide (GluCer) and their binding sites were identified. Our data will be useful to identify potential candidate genes and their role in host-plant resistance in chickpea, besides presenting opportunities for their potential for possible deployment in other crops.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s00344-022-10811-1</doi><tpages>17</tpages></addata></record> |
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| subjects | Agriculture Antimicrobial peptides Binding sites Bioassays Biomedical and Life Sciences Chickpeas chromosome mapping Cicer arietinum Defensins Dry rot Fungi Genes Genomes Genotypes glucosylceramides Host plants Innate immunity Legumes Life Sciences Localization Molecular docking Pathogens Peptides Phosphatidylserine phosphatidylserines Plant Anatomy/Development plant growth Plant Physiology Plant resistance Plant Sciences plasma membrane Regulatory sequences Root rot Soil resistance |
| title | Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil-Borne Chickpea Fungal Pathogens |
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