Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7

•The dendritic PDA-AuNPs display remarkable broadband absorption characteristics.•PDA-AuNPs with varying core sizes have impact on their ICA performance.•Absorption spectra of PDA-AuNPs overlap the excitation and emission spectra of QDs.•The colorimetric/fluorescent dual-mode immunoassay was establi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Food chemistry 2023-10, Vol.424, p.136366-136366, Article 136366
Hauptverfasser: Shao, Yanna, Wang, Zhengzheng, Xie, Jihang, Zhu, Zhenjun, Feng, Ying, Yu, Shubo, Xue, Liang, Wu, Shi, Gu, Qihui, Zhang, Jumei, Wu, Qingping, Wang, Juan, Ding, Yu
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 136366
container_issue
container_start_page 136366
container_title Food chemistry
container_volume 424
creator Shao, Yanna
Wang, Zhengzheng
Xie, Jihang
Zhu, Zhenjun
Feng, Ying
Yu, Shubo
Xue, Liang
Wu, Shi
Gu, Qihui
Zhang, Jumei
Wu, Qingping
Wang, Juan
Ding, Yu
description •The dendritic PDA-AuNPs display remarkable broadband absorption characteristics.•PDA-AuNPs with varying core sizes have impact on their ICA performance.•Absorption spectra of PDA-AuNPs overlap the excitation and emission spectra of QDs.•The colorimetric/fluorescent dual-mode immunoassay was established. We presented a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for the sensitive detection of Escherichia coli O157:H7. The use of polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption allowed for excellent colorimetry signals for the ICA detection. Moreover, the absorption spectrum of PDA-AuNPs significantly overlaps with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of the QDs fluorescence due to the inner filter effect. The fluorescence intensity changes induced by PDA-AuNPs were utilized for the sensitive detection of E. coli O157:H7, achieving a detection limit of 9.06 × 101 CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor exhibited the recovery rate between 80.12% and 114.69% in detecting actual samples, indicating its reliability and satisfactory accuracy. This study provides insights into dual-mode signal outputs and the ICA development for food safety applications.
doi_str_mv 10.1016/j.foodchem.2023.136366
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153179316</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0308814623009846</els_id><sourcerecordid>2816760042</sourcerecordid><originalsourceid>FETCH-LOGICAL-c401t-c622fbc7c8278caab46952da95077722236903ba7469fefdb18afbd3dcf40c313</originalsourceid><addsrcrecordid>eNqFUctu1DAUtRCIDoVfqLxkk9SPjJ1hBSqlRarUDawtx76eeOTYwU5a9W_4VDyalm1X93XuuVfnIHRBSUsJFZeH1qVkzQhTywjjLeWCC_EGbWgveSOJZG_RhnDSNz3txBn6UMqBEMII7d-jMy5r0km2QX-_rzo0U7KA_TStMZkxp0kvaZ_1PHqDdSn6CQ-6gMUpYgvRZr_UwT4Fi6OOada51gEKfvTLiMs6Q_YpYxfWlKEYiAbwn7WG0cc9dnW0hiVXxlgq0wNU0gXM4it9cvi6xSYFj-_pVn65lR_RO6dDgU_P8Rz9_nH96-q2ubu_-Xn17a4xHaFLYwRjbjDS9Ez2RuuhE7sts3q3JVJKxhgXO8IHLWvfgbMD7bUbLLfGdcRwys_R5xPvnFN9tixq8vX3EHSEtBbF6ZZTueNUvAplPRVSENKxChUnqMmplAxOzdlPOj8pStTRSHVQL0aqo5HqZGRdvHi-sQ4T2P9rL85VwNcTAKooDx6yKsYfpbY-VzGVTf61G_8AIke1ZA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2816760042</pqid></control><display><type>article</type><title>Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7</title><source>Elsevier ScienceDirect Journals</source><creator>Shao, Yanna ; Wang, Zhengzheng ; Xie, Jihang ; Zhu, Zhenjun ; Feng, Ying ; Yu, Shubo ; Xue, Liang ; Wu, Shi ; Gu, Qihui ; Zhang, Jumei ; Wu, Qingping ; Wang, Juan ; Ding, Yu</creator><creatorcontrib>Shao, Yanna ; Wang, Zhengzheng ; Xie, Jihang ; Zhu, Zhenjun ; Feng, Ying ; Yu, Shubo ; Xue, Liang ; Wu, Shi ; Gu, Qihui ; Zhang, Jumei ; Wu, Qingping ; Wang, Juan ; Ding, Yu</creatorcontrib><description>•The dendritic PDA-AuNPs display remarkable broadband absorption characteristics.•PDA-AuNPs with varying core sizes have impact on their ICA performance.•Absorption spectra of PDA-AuNPs overlap the excitation and emission spectra of QDs.•The colorimetric/fluorescent dual-mode immunoassay was established. We presented a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for the sensitive detection of Escherichia coli O157:H7. The use of polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption allowed for excellent colorimetry signals for the ICA detection. Moreover, the absorption spectrum of PDA-AuNPs significantly overlaps with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of the QDs fluorescence due to the inner filter effect. The fluorescence intensity changes induced by PDA-AuNPs were utilized for the sensitive detection of E. coli O157:H7, achieving a detection limit of 9.06 × 101 CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor exhibited the recovery rate between 80.12% and 114.69% in detecting actual samples, indicating its reliability and satisfactory accuracy. This study provides insights into dual-mode signal outputs and the ICA development for food safety applications.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2023.136366</identifier><identifier>PMID: 37201472</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>absorption ; Black nanoparticles ; Branched nanostructures ; colorimetry ; detection limit ; Dual-mode signal ; Escherichia coli O157 ; Escherichia coli O157:H7 ; fluorescence ; food chemistry ; food safety ; immunoaffinity chromatography ; immunoassays ; Immunochromatographic assay ; immunosensors ; Inner filter effect ; nanogold ; spectral analysis</subject><ispartof>Food chemistry, 2023-10, Vol.424, p.136366-136366, Article 136366</ispartof><rights>2023</rights><rights>Copyright © 2023. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-c622fbc7c8278caab46952da95077722236903ba7469fefdb18afbd3dcf40c313</citedby><cites>FETCH-LOGICAL-c401t-c622fbc7c8278caab46952da95077722236903ba7469fefdb18afbd3dcf40c313</cites><orcidid>0000-0002-3688-7294</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0308814623009846$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37201472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shao, Yanna</creatorcontrib><creatorcontrib>Wang, Zhengzheng</creatorcontrib><creatorcontrib>Xie, Jihang</creatorcontrib><creatorcontrib>Zhu, Zhenjun</creatorcontrib><creatorcontrib>Feng, Ying</creatorcontrib><creatorcontrib>Yu, Shubo</creatorcontrib><creatorcontrib>Xue, Liang</creatorcontrib><creatorcontrib>Wu, Shi</creatorcontrib><creatorcontrib>Gu, Qihui</creatorcontrib><creatorcontrib>Zhang, Jumei</creatorcontrib><creatorcontrib>Wu, Qingping</creatorcontrib><creatorcontrib>Wang, Juan</creatorcontrib><creatorcontrib>Ding, Yu</creatorcontrib><title>Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•The dendritic PDA-AuNPs display remarkable broadband absorption characteristics.•PDA-AuNPs with varying core sizes have impact on their ICA performance.•Absorption spectra of PDA-AuNPs overlap the excitation and emission spectra of QDs.•The colorimetric/fluorescent dual-mode immunoassay was established. We presented a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for the sensitive detection of Escherichia coli O157:H7. The use of polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption allowed for excellent colorimetry signals for the ICA detection. Moreover, the absorption spectrum of PDA-AuNPs significantly overlaps with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of the QDs fluorescence due to the inner filter effect. The fluorescence intensity changes induced by PDA-AuNPs were utilized for the sensitive detection of E. coli O157:H7, achieving a detection limit of 9.06 × 101 CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor exhibited the recovery rate between 80.12% and 114.69% in detecting actual samples, indicating its reliability and satisfactory accuracy. This study provides insights into dual-mode signal outputs and the ICA development for food safety applications.</description><subject>absorption</subject><subject>Black nanoparticles</subject><subject>Branched nanostructures</subject><subject>colorimetry</subject><subject>detection limit</subject><subject>Dual-mode signal</subject><subject>Escherichia coli O157</subject><subject>Escherichia coli O157:H7</subject><subject>fluorescence</subject><subject>food chemistry</subject><subject>food safety</subject><subject>immunoaffinity chromatography</subject><subject>immunoassays</subject><subject>Immunochromatographic assay</subject><subject>immunosensors</subject><subject>Inner filter effect</subject><subject>nanogold</subject><subject>spectral analysis</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFUctu1DAUtRCIDoVfqLxkk9SPjJ1hBSqlRarUDawtx76eeOTYwU5a9W_4VDyalm1X93XuuVfnIHRBSUsJFZeH1qVkzQhTywjjLeWCC_EGbWgveSOJZG_RhnDSNz3txBn6UMqBEMII7d-jMy5r0km2QX-_rzo0U7KA_TStMZkxp0kvaZ_1PHqDdSn6CQ-6gMUpYgvRZr_UwT4Fi6OOada51gEKfvTLiMs6Q_YpYxfWlKEYiAbwn7WG0cc9dnW0hiVXxlgq0wNU0gXM4it9cvi6xSYFj-_pVn65lR_RO6dDgU_P8Rz9_nH96-q2ubu_-Xn17a4xHaFLYwRjbjDS9Ez2RuuhE7sts3q3JVJKxhgXO8IHLWvfgbMD7bUbLLfGdcRwys_R5xPvnFN9tixq8vX3EHSEtBbF6ZZTueNUvAplPRVSENKxChUnqMmplAxOzdlPOj8pStTRSHVQL0aqo5HqZGRdvHi-sQ4T2P9rL85VwNcTAKooDx6yKsYfpbY-VzGVTf61G_8AIke1ZA</recordid><startdate>20231030</startdate><enddate>20231030</enddate><creator>Shao, Yanna</creator><creator>Wang, Zhengzheng</creator><creator>Xie, Jihang</creator><creator>Zhu, Zhenjun</creator><creator>Feng, Ying</creator><creator>Yu, Shubo</creator><creator>Xue, Liang</creator><creator>Wu, Shi</creator><creator>Gu, Qihui</creator><creator>Zhang, Jumei</creator><creator>Wu, Qingping</creator><creator>Wang, Juan</creator><creator>Ding, Yu</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-3688-7294</orcidid></search><sort><creationdate>20231030</creationdate><title>Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7</title><author>Shao, Yanna ; Wang, Zhengzheng ; Xie, Jihang ; Zhu, Zhenjun ; Feng, Ying ; Yu, Shubo ; Xue, Liang ; Wu, Shi ; Gu, Qihui ; Zhang, Jumei ; Wu, Qingping ; Wang, Juan ; Ding, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-c622fbc7c8278caab46952da95077722236903ba7469fefdb18afbd3dcf40c313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>absorption</topic><topic>Black nanoparticles</topic><topic>Branched nanostructures</topic><topic>colorimetry</topic><topic>detection limit</topic><topic>Dual-mode signal</topic><topic>Escherichia coli O157</topic><topic>Escherichia coli O157:H7</topic><topic>fluorescence</topic><topic>food chemistry</topic><topic>food safety</topic><topic>immunoaffinity chromatography</topic><topic>immunoassays</topic><topic>Immunochromatographic assay</topic><topic>immunosensors</topic><topic>Inner filter effect</topic><topic>nanogold</topic><topic>spectral analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shao, Yanna</creatorcontrib><creatorcontrib>Wang, Zhengzheng</creatorcontrib><creatorcontrib>Xie, Jihang</creatorcontrib><creatorcontrib>Zhu, Zhenjun</creatorcontrib><creatorcontrib>Feng, Ying</creatorcontrib><creatorcontrib>Yu, Shubo</creatorcontrib><creatorcontrib>Xue, Liang</creatorcontrib><creatorcontrib>Wu, Shi</creatorcontrib><creatorcontrib>Gu, Qihui</creatorcontrib><creatorcontrib>Zhang, Jumei</creatorcontrib><creatorcontrib>Wu, Qingping</creatorcontrib><creatorcontrib>Wang, Juan</creatorcontrib><creatorcontrib>Ding, Yu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shao, Yanna</au><au>Wang, Zhengzheng</au><au>Xie, Jihang</au><au>Zhu, Zhenjun</au><au>Feng, Ying</au><au>Yu, Shubo</au><au>Xue, Liang</au><au>Wu, Shi</au><au>Gu, Qihui</au><au>Zhang, Jumei</au><au>Wu, Qingping</au><au>Wang, Juan</au><au>Ding, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2023-10-30</date><risdate>2023</risdate><volume>424</volume><spage>136366</spage><epage>136366</epage><pages>136366-136366</pages><artnum>136366</artnum><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>•The dendritic PDA-AuNPs display remarkable broadband absorption characteristics.•PDA-AuNPs with varying core sizes have impact on their ICA performance.•Absorption spectra of PDA-AuNPs overlap the excitation and emission spectra of QDs.•The colorimetric/fluorescent dual-mode immunoassay was established. We presented a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for the sensitive detection of Escherichia coli O157:H7. The use of polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption allowed for excellent colorimetry signals for the ICA detection. Moreover, the absorption spectrum of PDA-AuNPs significantly overlaps with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of the QDs fluorescence due to the inner filter effect. The fluorescence intensity changes induced by PDA-AuNPs were utilized for the sensitive detection of E. coli O157:H7, achieving a detection limit of 9.06 × 101 CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor exhibited the recovery rate between 80.12% and 114.69% in detecting actual samples, indicating its reliability and satisfactory accuracy. This study provides insights into dual-mode signal outputs and the ICA development for food safety applications.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>37201472</pmid><doi>10.1016/j.foodchem.2023.136366</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-3688-7294</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0308-8146
ispartof Food chemistry, 2023-10, Vol.424, p.136366-136366, Article 136366
issn 0308-8146
1873-7072
language eng
recordid cdi_proquest_miscellaneous_3153179316
source Elsevier ScienceDirect Journals
subjects absorption
Black nanoparticles
Branched nanostructures
colorimetry
detection limit
Dual-mode signal
Escherichia coli O157
Escherichia coli O157:H7
fluorescence
food chemistry
food safety
immunoaffinity chromatography
immunoassays
Immunochromatographic assay
immunosensors
Inner filter effect
nanogold
spectral analysis
title Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T04%3A56%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dual-mode%20immunochromatographic%20assay%20based%20on%20dendritic%20gold%20nanoparticles%20with%20superior%20fluorescence%20quenching%20for%20ultrasensitive%20detection%20of%20E.%20coli%20O157:H7&rft.jtitle=Food%20chemistry&rft.au=Shao,%20Yanna&rft.date=2023-10-30&rft.volume=424&rft.spage=136366&rft.epage=136366&rft.pages=136366-136366&rft.artnum=136366&rft.issn=0308-8146&rft.eissn=1873-7072&rft_id=info:doi/10.1016/j.foodchem.2023.136366&rft_dat=%3Cproquest_cross%3E2816760042%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2816760042&rft_id=info:pmid/37201472&rft_els_id=S0308814623009846&rfr_iscdi=true