Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7
•The dendritic PDA-AuNPs display remarkable broadband absorption characteristics.•PDA-AuNPs with varying core sizes have impact on their ICA performance.•Absorption spectra of PDA-AuNPs overlap the excitation and emission spectra of QDs.•The colorimetric/fluorescent dual-mode immunoassay was establi...
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Veröffentlicht in: | Food chemistry 2023-10, Vol.424, p.136366-136366, Article 136366 |
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creator | Shao, Yanna Wang, Zhengzheng Xie, Jihang Zhu, Zhenjun Feng, Ying Yu, Shubo Xue, Liang Wu, Shi Gu, Qihui Zhang, Jumei Wu, Qingping Wang, Juan Ding, Yu |
description | •The dendritic PDA-AuNPs display remarkable broadband absorption characteristics.•PDA-AuNPs with varying core sizes have impact on their ICA performance.•Absorption spectra of PDA-AuNPs overlap the excitation and emission spectra of QDs.•The colorimetric/fluorescent dual-mode immunoassay was established.
We presented a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for the sensitive detection of Escherichia coli O157:H7. The use of polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption allowed for excellent colorimetry signals for the ICA detection. Moreover, the absorption spectrum of PDA-AuNPs significantly overlaps with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of the QDs fluorescence due to the inner filter effect. The fluorescence intensity changes induced by PDA-AuNPs were utilized for the sensitive detection of E. coli O157:H7, achieving a detection limit of 9.06 × 101 CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor exhibited the recovery rate between 80.12% and 114.69% in detecting actual samples, indicating its reliability and satisfactory accuracy. This study provides insights into dual-mode signal outputs and the ICA development for food safety applications. |
doi_str_mv | 10.1016/j.foodchem.2023.136366 |
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We presented a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for the sensitive detection of Escherichia coli O157:H7. The use of polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption allowed for excellent colorimetry signals for the ICA detection. Moreover, the absorption spectrum of PDA-AuNPs significantly overlaps with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of the QDs fluorescence due to the inner filter effect. The fluorescence intensity changes induced by PDA-AuNPs were utilized for the sensitive detection of E. coli O157:H7, achieving a detection limit of 9.06 × 101 CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor exhibited the recovery rate between 80.12% and 114.69% in detecting actual samples, indicating its reliability and satisfactory accuracy. This study provides insights into dual-mode signal outputs and the ICA development for food safety applications.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2023.136366</identifier><identifier>PMID: 37201472</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>absorption ; Black nanoparticles ; Branched nanostructures ; colorimetry ; detection limit ; Dual-mode signal ; Escherichia coli O157 ; Escherichia coli O157:H7 ; fluorescence ; food chemistry ; food safety ; immunoaffinity chromatography ; immunoassays ; Immunochromatographic assay ; immunosensors ; Inner filter effect ; nanogold ; spectral analysis</subject><ispartof>Food chemistry, 2023-10, Vol.424, p.136366-136366, Article 136366</ispartof><rights>2023</rights><rights>Copyright © 2023. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-c622fbc7c8278caab46952da95077722236903ba7469fefdb18afbd3dcf40c313</citedby><cites>FETCH-LOGICAL-c401t-c622fbc7c8278caab46952da95077722236903ba7469fefdb18afbd3dcf40c313</cites><orcidid>0000-0002-3688-7294</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0308814623009846$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37201472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shao, Yanna</creatorcontrib><creatorcontrib>Wang, Zhengzheng</creatorcontrib><creatorcontrib>Xie, Jihang</creatorcontrib><creatorcontrib>Zhu, Zhenjun</creatorcontrib><creatorcontrib>Feng, Ying</creatorcontrib><creatorcontrib>Yu, Shubo</creatorcontrib><creatorcontrib>Xue, Liang</creatorcontrib><creatorcontrib>Wu, Shi</creatorcontrib><creatorcontrib>Gu, Qihui</creatorcontrib><creatorcontrib>Zhang, Jumei</creatorcontrib><creatorcontrib>Wu, Qingping</creatorcontrib><creatorcontrib>Wang, Juan</creatorcontrib><creatorcontrib>Ding, Yu</creatorcontrib><title>Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•The dendritic PDA-AuNPs display remarkable broadband absorption characteristics.•PDA-AuNPs with varying core sizes have impact on their ICA performance.•Absorption spectra of PDA-AuNPs overlap the excitation and emission spectra of QDs.•The colorimetric/fluorescent dual-mode immunoassay was established.
We presented a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for the sensitive detection of Escherichia coli O157:H7. The use of polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption allowed for excellent colorimetry signals for the ICA detection. Moreover, the absorption spectrum of PDA-AuNPs significantly overlaps with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of the QDs fluorescence due to the inner filter effect. The fluorescence intensity changes induced by PDA-AuNPs were utilized for the sensitive detection of E. coli O157:H7, achieving a detection limit of 9.06 × 101 CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor exhibited the recovery rate between 80.12% and 114.69% in detecting actual samples, indicating its reliability and satisfactory accuracy. This study provides insights into dual-mode signal outputs and the ICA development for food safety applications.</description><subject>absorption</subject><subject>Black nanoparticles</subject><subject>Branched nanostructures</subject><subject>colorimetry</subject><subject>detection limit</subject><subject>Dual-mode signal</subject><subject>Escherichia coli O157</subject><subject>Escherichia coli O157:H7</subject><subject>fluorescence</subject><subject>food chemistry</subject><subject>food safety</subject><subject>immunoaffinity chromatography</subject><subject>immunoassays</subject><subject>Immunochromatographic assay</subject><subject>immunosensors</subject><subject>Inner filter effect</subject><subject>nanogold</subject><subject>spectral analysis</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFUctu1DAUtRCIDoVfqLxkk9SPjJ1hBSqlRarUDawtx76eeOTYwU5a9W_4VDyalm1X93XuuVfnIHRBSUsJFZeH1qVkzQhTywjjLeWCC_EGbWgveSOJZG_RhnDSNz3txBn6UMqBEMII7d-jMy5r0km2QX-_rzo0U7KA_TStMZkxp0kvaZ_1PHqDdSn6CQ-6gMUpYgvRZr_UwT4Fi6OOada51gEKfvTLiMs6Q_YpYxfWlKEYiAbwn7WG0cc9dnW0hiVXxlgq0wNU0gXM4it9cvi6xSYFj-_pVn65lR_RO6dDgU_P8Rz9_nH96-q2ubu_-Xn17a4xHaFLYwRjbjDS9Ez2RuuhE7sts3q3JVJKxhgXO8IHLWvfgbMD7bUbLLfGdcRwys_R5xPvnFN9tixq8vX3EHSEtBbF6ZZTueNUvAplPRVSENKxChUnqMmplAxOzdlPOj8pStTRSHVQL0aqo5HqZGRdvHi-sQ4T2P9rL85VwNcTAKooDx6yKsYfpbY-VzGVTf61G_8AIke1ZA</recordid><startdate>20231030</startdate><enddate>20231030</enddate><creator>Shao, Yanna</creator><creator>Wang, Zhengzheng</creator><creator>Xie, Jihang</creator><creator>Zhu, Zhenjun</creator><creator>Feng, Ying</creator><creator>Yu, Shubo</creator><creator>Xue, Liang</creator><creator>Wu, Shi</creator><creator>Gu, Qihui</creator><creator>Zhang, Jumei</creator><creator>Wu, Qingping</creator><creator>Wang, Juan</creator><creator>Ding, Yu</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-3688-7294</orcidid></search><sort><creationdate>20231030</creationdate><title>Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7</title><author>Shao, Yanna ; Wang, Zhengzheng ; Xie, Jihang ; Zhu, Zhenjun ; Feng, Ying ; Yu, Shubo ; Xue, Liang ; Wu, Shi ; Gu, Qihui ; Zhang, Jumei ; Wu, Qingping ; Wang, Juan ; Ding, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-c622fbc7c8278caab46952da95077722236903ba7469fefdb18afbd3dcf40c313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>absorption</topic><topic>Black nanoparticles</topic><topic>Branched nanostructures</topic><topic>colorimetry</topic><topic>detection limit</topic><topic>Dual-mode signal</topic><topic>Escherichia coli O157</topic><topic>Escherichia coli O157:H7</topic><topic>fluorescence</topic><topic>food chemistry</topic><topic>food safety</topic><topic>immunoaffinity chromatography</topic><topic>immunoassays</topic><topic>Immunochromatographic assay</topic><topic>immunosensors</topic><topic>Inner filter effect</topic><topic>nanogold</topic><topic>spectral analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shao, Yanna</creatorcontrib><creatorcontrib>Wang, Zhengzheng</creatorcontrib><creatorcontrib>Xie, Jihang</creatorcontrib><creatorcontrib>Zhu, Zhenjun</creatorcontrib><creatorcontrib>Feng, Ying</creatorcontrib><creatorcontrib>Yu, Shubo</creatorcontrib><creatorcontrib>Xue, Liang</creatorcontrib><creatorcontrib>Wu, Shi</creatorcontrib><creatorcontrib>Gu, Qihui</creatorcontrib><creatorcontrib>Zhang, Jumei</creatorcontrib><creatorcontrib>Wu, Qingping</creatorcontrib><creatorcontrib>Wang, Juan</creatorcontrib><creatorcontrib>Ding, Yu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shao, Yanna</au><au>Wang, Zhengzheng</au><au>Xie, Jihang</au><au>Zhu, Zhenjun</au><au>Feng, Ying</au><au>Yu, Shubo</au><au>Xue, Liang</au><au>Wu, Shi</au><au>Gu, Qihui</au><au>Zhang, Jumei</au><au>Wu, Qingping</au><au>Wang, Juan</au><au>Ding, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2023-10-30</date><risdate>2023</risdate><volume>424</volume><spage>136366</spage><epage>136366</epage><pages>136366-136366</pages><artnum>136366</artnum><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>•The dendritic PDA-AuNPs display remarkable broadband absorption characteristics.•PDA-AuNPs with varying core sizes have impact on their ICA performance.•Absorption spectra of PDA-AuNPs overlap the excitation and emission spectra of QDs.•The colorimetric/fluorescent dual-mode immunoassay was established.
We presented a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for the sensitive detection of Escherichia coli O157:H7. The use of polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption allowed for excellent colorimetry signals for the ICA detection. Moreover, the absorption spectrum of PDA-AuNPs significantly overlaps with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of the QDs fluorescence due to the inner filter effect. The fluorescence intensity changes induced by PDA-AuNPs were utilized for the sensitive detection of E. coli O157:H7, achieving a detection limit of 9.06 × 101 CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor exhibited the recovery rate between 80.12% and 114.69% in detecting actual samples, indicating its reliability and satisfactory accuracy. This study provides insights into dual-mode signal outputs and the ICA development for food safety applications.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>37201472</pmid><doi>10.1016/j.foodchem.2023.136366</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-3688-7294</orcidid></addata></record> |
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subjects | absorption Black nanoparticles Branched nanostructures colorimetry detection limit Dual-mode signal Escherichia coli O157 Escherichia coli O157:H7 fluorescence food chemistry food safety immunoaffinity chromatography immunoassays Immunochromatographic assay immunosensors Inner filter effect nanogold spectral analysis |
title | Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7 |
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