Effect of sweetened beverages intake on salivary aspartame, insulin and alpha-amylase levels: A single-blind study
[Display omitted] •This is the first study that report aspartame excretion in saliva.•Greater salivary aspartame area under the peak was observed after Diet soft drink compared to Water + sweeteners intake.•Higher salivary insulin levels were observed after regular and diet soft drinks compared to l...
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| Veröffentlicht in: | Food research international 2023-11, Vol.173, p.113406-113406, Article 113406 |
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| creator | Finassi, Carolina Martins Calixto, Leandro A. Segura, Wilson Bocato, Mariana Zuccherato Barbosa Júnior, Fernando Fonseca, Fernando L.A. Lamy, Elsa Castelo, Paula Midori |
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•This is the first study that report aspartame excretion in saliva.•Greater salivary aspartame area under the peak was observed after Diet soft drink compared to Water + sweeteners intake.•Higher salivary insulin levels were observed after regular and diet soft drinks compared to low sucrose and water test-drinks ingestion.
The objective was to assess aspartame excretion in saliva and the salivary insulin, total protein (TP), and alpha-amylase (AMI) levels in response to the ingestion of sweetened beverages (sodium cyclamate, aspartame, acesulfame, and sucrose). Fifteen healthy participants were included in a single-blinded trial with the intake of Diet soft drink, Regular soft drink, Water + sweeteners, Low sucrose content (3.5 g), and Water (blank) in 5 different days. In each day, saliva was collected at T0 (fasting), T1 (15 min after test-drink intake), T2 (30 min), T3 (60 min), and T4 (120 min) for the measurement of salivary aspartame (HPLC), TP, AMI (ELISA assays) and insulin levels (chemiluminescence). Chi-square, Friedman, ANOVA and Spearman correlation tests were applied. The late-perceived sweet/sour residual flavor was reported at a frequency of 80%, 60% and 20% after ingestion of artificially sweetened drinks, beverages with sucrose, and plain water, respectively (p |
| doi_str_mv | 10.1016/j.foodres.2023.113406 |
| format | Article |
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•This is the first study that report aspartame excretion in saliva.•Greater salivary aspartame area under the peak was observed after Diet soft drink compared to Water + sweeteners intake.•Higher salivary insulin levels were observed after regular and diet soft drinks compared to low sucrose and water test-drinks ingestion.
The objective was to assess aspartame excretion in saliva and the salivary insulin, total protein (TP), and alpha-amylase (AMI) levels in response to the ingestion of sweetened beverages (sodium cyclamate, aspartame, acesulfame, and sucrose). Fifteen healthy participants were included in a single-blinded trial with the intake of Diet soft drink, Regular soft drink, Water + sweeteners, Low sucrose content (3.5 g), and Water (blank) in 5 different days. In each day, saliva was collected at T0 (fasting), T1 (15 min after test-drink intake), T2 (30 min), T3 (60 min), and T4 (120 min) for the measurement of salivary aspartame (HPLC), TP, AMI (ELISA assays) and insulin levels (chemiluminescence). Chi-square, Friedman, ANOVA and Spearman correlation tests were applied. The late-perceived sweet/sour residual flavor was reported at a frequency of 80%, 60% and 20% after ingestion of artificially sweetened drinks, beverages with sucrose, and plain water, respectively (p < 0.05). Aspartame was detected in saliva after artificially sweetened drinks intake, with highest area under the peak for the Diet soft drink (p = 0.014). No change was observed for TP and AMI levels during the 120 min. Insulin levels increased 1 h after soft-drinks ingestion (regular and diet), while the levels did not change for Low sucrose content and Water + sweeteners test-drinks. Salivary aspartame correlated with insulin levels only after Diet soft drink intake (rho ≥ 0.7; p < 0.05). As aspartame can be detected in saliva and swallowed again until completely excreted, these results contribute to the knowledge of the biological fate of artificial sweeteners and the study of health outcomes.</description><identifier>ISSN: 0963-9969</identifier><identifier>EISSN: 1873-7145</identifier><identifier>DOI: 10.1016/j.foodres.2023.113406</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>acesulfame potassium ; alpha-amylase ; Amylase ; Aspartame ; chemiluminescence ; diet ; excretion ; fasting ; flavor ; food research ; ingestion ; Insulin ; protein content ; Saliva ; sodium cyclamate ; Soft drink ; soft drinks ; sucrose ; Sweetening Agents</subject><ispartof>Food research international, 2023-11, Vol.173, p.113406-113406, Article 113406</ispartof><rights>2023 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-9c32b21b8507ee28327e2a699be196c1aac5a7b4a8dbd138c867190e826bccee3</citedby><cites>FETCH-LOGICAL-c375t-9c32b21b8507ee28327e2a699be196c1aac5a7b4a8dbd138c867190e826bccee3</cites><orcidid>0000-0003-1134-5760 ; 0000-0003-1776-7470</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0963996923009511$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids></links><search><creatorcontrib>Finassi, Carolina Martins</creatorcontrib><creatorcontrib>Calixto, Leandro A.</creatorcontrib><creatorcontrib>Segura, Wilson</creatorcontrib><creatorcontrib>Bocato, Mariana Zuccherato</creatorcontrib><creatorcontrib>Barbosa Júnior, Fernando</creatorcontrib><creatorcontrib>Fonseca, Fernando L.A.</creatorcontrib><creatorcontrib>Lamy, Elsa</creatorcontrib><creatorcontrib>Castelo, Paula Midori</creatorcontrib><title>Effect of sweetened beverages intake on salivary aspartame, insulin and alpha-amylase levels: A single-blind study</title><title>Food research international</title><description>[Display omitted]
•This is the first study that report aspartame excretion in saliva.•Greater salivary aspartame area under the peak was observed after Diet soft drink compared to Water + sweeteners intake.•Higher salivary insulin levels were observed after regular and diet soft drinks compared to low sucrose and water test-drinks ingestion.
The objective was to assess aspartame excretion in saliva and the salivary insulin, total protein (TP), and alpha-amylase (AMI) levels in response to the ingestion of sweetened beverages (sodium cyclamate, aspartame, acesulfame, and sucrose). Fifteen healthy participants were included in a single-blinded trial with the intake of Diet soft drink, Regular soft drink, Water + sweeteners, Low sucrose content (3.5 g), and Water (blank) in 5 different days. In each day, saliva was collected at T0 (fasting), T1 (15 min after test-drink intake), T2 (30 min), T3 (60 min), and T4 (120 min) for the measurement of salivary aspartame (HPLC), TP, AMI (ELISA assays) and insulin levels (chemiluminescence). Chi-square, Friedman, ANOVA and Spearman correlation tests were applied. The late-perceived sweet/sour residual flavor was reported at a frequency of 80%, 60% and 20% after ingestion of artificially sweetened drinks, beverages with sucrose, and plain water, respectively (p < 0.05). Aspartame was detected in saliva after artificially sweetened drinks intake, with highest area under the peak for the Diet soft drink (p = 0.014). No change was observed for TP and AMI levels during the 120 min. Insulin levels increased 1 h after soft-drinks ingestion (regular and diet), while the levels did not change for Low sucrose content and Water + sweeteners test-drinks. Salivary aspartame correlated with insulin levels only after Diet soft drink intake (rho ≥ 0.7; p < 0.05). As aspartame can be detected in saliva and swallowed again until completely excreted, these results contribute to the knowledge of the biological fate of artificial sweeteners and the study of health outcomes.</description><subject>acesulfame potassium</subject><subject>alpha-amylase</subject><subject>Amylase</subject><subject>Aspartame</subject><subject>chemiluminescence</subject><subject>diet</subject><subject>excretion</subject><subject>fasting</subject><subject>flavor</subject><subject>food research</subject><subject>ingestion</subject><subject>Insulin</subject><subject>protein content</subject><subject>Saliva</subject><subject>sodium cyclamate</subject><subject>Soft drink</subject><subject>soft drinks</subject><subject>sucrose</subject><subject>Sweetening Agents</subject><issn>0963-9969</issn><issn>1873-7145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFkUtPGzEUha2KSg2Un1DJyy46wY-MH2wqhKBFQmIDa-uOfYc6dWaC7yQo_55BYc_qLu53zuJ8jP2QYimFNBfrZT-OqSItlVB6KaVeCfOFLaSzurFy1Z6whfBGN94b_42dEq2FEKa1fsHqTd9jnPjYc3pFnHDAxDvcY4VnJJ6HCf4jHwdOUPIe6oEDbaFOsMFf85d2JQ8chsShbP9BA5tDAUJe5oZCl_yKUx6eCzbdzCVO0y4dvrOvPRTC8497xp5ubx6v_zb3D3_urq_um6htOzU-atUp2blWWETltLKowHjfofQmSoDYgu1W4FKXpHbRGSu9QKdMFyOiPmM_j73bOr7skKawyRSxFBhw3FHQstXStE6JT1Hl7EoZ7ZWa0faIxjoSVezDtubNPEyQIrzrCOvwoSO86whHHXPu9zE374L7jDVQzDhETLnOAkIa8ycNbxPTl2w</recordid><startdate>20231101</startdate><enddate>20231101</enddate><creator>Finassi, Carolina Martins</creator><creator>Calixto, Leandro A.</creator><creator>Segura, Wilson</creator><creator>Bocato, Mariana Zuccherato</creator><creator>Barbosa Júnior, Fernando</creator><creator>Fonseca, Fernando L.A.</creator><creator>Lamy, Elsa</creator><creator>Castelo, Paula Midori</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-1134-5760</orcidid><orcidid>https://orcid.org/0000-0003-1776-7470</orcidid></search><sort><creationdate>20231101</creationdate><title>Effect of sweetened beverages intake on salivary aspartame, insulin and alpha-amylase levels: A single-blind study</title><author>Finassi, Carolina Martins ; Calixto, Leandro A. ; Segura, Wilson ; Bocato, Mariana Zuccherato ; Barbosa Júnior, Fernando ; Fonseca, Fernando L.A. ; Lamy, Elsa ; Castelo, Paula Midori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-9c32b21b8507ee28327e2a699be196c1aac5a7b4a8dbd138c867190e826bccee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>acesulfame potassium</topic><topic>alpha-amylase</topic><topic>Amylase</topic><topic>Aspartame</topic><topic>chemiluminescence</topic><topic>diet</topic><topic>excretion</topic><topic>fasting</topic><topic>flavor</topic><topic>food research</topic><topic>ingestion</topic><topic>Insulin</topic><topic>protein content</topic><topic>Saliva</topic><topic>sodium cyclamate</topic><topic>Soft drink</topic><topic>soft drinks</topic><topic>sucrose</topic><topic>Sweetening Agents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Finassi, Carolina Martins</creatorcontrib><creatorcontrib>Calixto, Leandro A.</creatorcontrib><creatorcontrib>Segura, Wilson</creatorcontrib><creatorcontrib>Bocato, Mariana Zuccherato</creatorcontrib><creatorcontrib>Barbosa Júnior, Fernando</creatorcontrib><creatorcontrib>Fonseca, Fernando L.A.</creatorcontrib><creatorcontrib>Lamy, Elsa</creatorcontrib><creatorcontrib>Castelo, Paula Midori</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Food research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Finassi, Carolina Martins</au><au>Calixto, Leandro A.</au><au>Segura, Wilson</au><au>Bocato, Mariana Zuccherato</au><au>Barbosa Júnior, Fernando</au><au>Fonseca, Fernando L.A.</au><au>Lamy, Elsa</au><au>Castelo, Paula Midori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of sweetened beverages intake on salivary aspartame, insulin and alpha-amylase levels: A single-blind study</atitle><jtitle>Food research international</jtitle><date>2023-11-01</date><risdate>2023</risdate><volume>173</volume><spage>113406</spage><epage>113406</epage><pages>113406-113406</pages><artnum>113406</artnum><issn>0963-9969</issn><eissn>1873-7145</eissn><abstract>[Display omitted]
•This is the first study that report aspartame excretion in saliva.•Greater salivary aspartame area under the peak was observed after Diet soft drink compared to Water + sweeteners intake.•Higher salivary insulin levels were observed after regular and diet soft drinks compared to low sucrose and water test-drinks ingestion.
The objective was to assess aspartame excretion in saliva and the salivary insulin, total protein (TP), and alpha-amylase (AMI) levels in response to the ingestion of sweetened beverages (sodium cyclamate, aspartame, acesulfame, and sucrose). Fifteen healthy participants were included in a single-blinded trial with the intake of Diet soft drink, Regular soft drink, Water + sweeteners, Low sucrose content (3.5 g), and Water (blank) in 5 different days. In each day, saliva was collected at T0 (fasting), T1 (15 min after test-drink intake), T2 (30 min), T3 (60 min), and T4 (120 min) for the measurement of salivary aspartame (HPLC), TP, AMI (ELISA assays) and insulin levels (chemiluminescence). Chi-square, Friedman, ANOVA and Spearman correlation tests were applied. The late-perceived sweet/sour residual flavor was reported at a frequency of 80%, 60% and 20% after ingestion of artificially sweetened drinks, beverages with sucrose, and plain water, respectively (p < 0.05). Aspartame was detected in saliva after artificially sweetened drinks intake, with highest area under the peak for the Diet soft drink (p = 0.014). No change was observed for TP and AMI levels during the 120 min. Insulin levels increased 1 h after soft-drinks ingestion (regular and diet), while the levels did not change for Low sucrose content and Water + sweeteners test-drinks. Salivary aspartame correlated with insulin levels only after Diet soft drink intake (rho ≥ 0.7; p < 0.05). As aspartame can be detected in saliva and swallowed again until completely excreted, these results contribute to the knowledge of the biological fate of artificial sweeteners and the study of health outcomes.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.foodres.2023.113406</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1134-5760</orcidid><orcidid>https://orcid.org/0000-0003-1776-7470</orcidid></addata></record> |
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| subjects | acesulfame potassium alpha-amylase Amylase Aspartame chemiluminescence diet excretion fasting flavor food research ingestion Insulin protein content Saliva sodium cyclamate Soft drink soft drinks sucrose Sweetening Agents |
| title | Effect of sweetened beverages intake on salivary aspartame, insulin and alpha-amylase levels: A single-blind study |
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