Overexpression of Bactericidal/Permeability-Increasing Fold-Containing Family BPIFB1 in Gastric Cancer Cells Leads to Differential Expression of E-Cadherin and MUC5AC
The human Bactericidal/Permeability-Increasing Fold containing family B, member 1 (BPIFB1), or also known as LPLUNC1, C20orf114, is a member of the BPI/LBP/PLUNC protein family. BPIFB1 differential expression patterns have been reported in several cancers such as nasopharyngeal carcinoma (NPC). Howe...
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| Veröffentlicht in: | Biology bulletin of the Russian Academy of Sciences 2023-08, Vol.50 (4), p.532-545 |
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| creator | Nor Azlin Safina Abdul Aziz Musa, Maslinda Kadir, Siti Hamimah Sheikh Abdul Karim, Zeti Rahayu Abdul Hasani, Narimah Abdul Hamid |
| description | The human Bactericidal/Permeability-Increasing Fold containing family B, member 1 (BPIFB1), or also known as LPLUNC1, C20orf114, is a member of the BPI/LBP/PLUNC protein family.
BPIFB1
differential expression patterns have been reported in several cancers such as nasopharyngeal carcinoma (NPC). However, in gastric cancer (GC) the role of
BPIFB1
is not known, but the expression of the gene has been reported in the stomach co-expressing E-cadherin (
CDH1
) together with making mucin 5, tracheobronchial/gastric mucin type A and C (
MUC5AC
). To investigate the role of
BPIFB1
in GC development and its interactions with
CDH1
and
MUC5AC
, overexpression of the gene was induced in three different GC cell lines; AGS, HGC-27 and MKN-45. The present of
BPIFB1
protein in a transfected GC cell line was detected through gel Lumio Green Detection.
BPIFB1
,
CDH1
and
MUC5AC
expression were then performed via RT-PCR and quantitative Real-time PCR (qPCR) followed by statistical analysis (
p
≤ 0.05).
BPIFB1
expression was detected in all transfected cells with the highest in AGS with the
p
= 0.003 followed by MKN-45 with the
p
= 0.009 and HGC-27 with the
p
= 0.015. The expression of
MUC5AC
is upregulated in
BPIFB1
transfected GC cells whereby the highest expression is observed in
MKN-45
with the
p
= 0.05 followed by AGS with the
p
= 0.001 and HGC-27 with the
p
= 0.026.
CDH1
expression is downregulated in all
BPIFB1
transfected GC cells with the lowest expression found in HGC-27 with the
p
= 0.001 followed by AGS with the
p
= 0.003 and MKN-45 with the
p
= 0.004. Thus, induction of
BPIFB1
overexpression in vitro in GC cells contributes to the decreased in
CDH1
expression and increased in
MUC5AC
expression suggesting that
BPIFB1
other than co-expressed in the gastric gland with the two genes also regulates gene expression. We found that the overexpression of
BPIFB1
downregulated the expression of
CDH1
and upregulated
MUC5AC
gene expression. This finding was supported by both RT-PCR and qPCR. Conclusion: Our findings suggest that
BPIFB1
is differentially expressed in GC cell lines and regulate the expression of
CDH1
and
MUC5AC
. |
| doi_str_mv | 10.1134/S1062359022601756 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153160893</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2851197526</sourcerecordid><originalsourceid>FETCH-LOGICAL-c301t-d195ea6a70e1f35410afdb21a05e409d6bbc7d48f8b55dce49635a92d18bebb23</originalsourceid><addsrcrecordid>eNp1kc9O3DAQxqOKSuXfA_RmqZdeUjx2nE2ObNiFlbYCqXCOJvaEGmXtxc6i7gvxnHhZJNRWnMaj-X3ffPJk2VfgPwBkcfYLeCmkqrkQJYeJKj9lh1DyKpdc1Qfpncb5bv4lO4rxgXNeyEIcZs_XTxTozzpQjNY75ns2RT1SsNoaHM5uKKwIOzvYcZsvnA6E0bp7NveDyRvvRrTutceVHbZserOYT4FZxy4xjsmENeg0BdbQMES2JDSRjZ5d2L5Pe91ocWCzv9bP8gbN7xTAMXSG_bxr1Hlzkn3ucYh0-laPs7v57La5ypfXl4vmfJlryWHMDdSKsMQJJ-ilKoBjbzoByBUVvDZl1-mJKaq-6pQymoq6lAprYaDqqOuEPM6-733XwT9uKI7tykadsqMjv4mtBCV331rLhH77B33wm-BSulZUCqCeKFEmCvaUDj7GQH27DnaFYdsCb3eXa_-7XNKIvSYm1t1TeHf-WPQC6E6bLw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2851197526</pqid></control><display><type>article</type><title>Overexpression of Bactericidal/Permeability-Increasing Fold-Containing Family BPIFB1 in Gastric Cancer Cells Leads to Differential Expression of E-Cadherin and MUC5AC</title><source>Springer Nature - Complete Springer Journals</source><creator>Nor Azlin Safina Abdul Aziz ; Musa, Maslinda ; Kadir, Siti Hamimah Sheikh Abdul ; Karim, Zeti Rahayu Abdul ; Hasani, Narimah Abdul Hamid</creator><creatorcontrib>Nor Azlin Safina Abdul Aziz ; Musa, Maslinda ; Kadir, Siti Hamimah Sheikh Abdul ; Karim, Zeti Rahayu Abdul ; Hasani, Narimah Abdul Hamid</creatorcontrib><description>The human Bactericidal/Permeability-Increasing Fold containing family B, member 1 (BPIFB1), or also known as LPLUNC1, C20orf114, is a member of the BPI/LBP/PLUNC protein family.
BPIFB1
differential expression patterns have been reported in several cancers such as nasopharyngeal carcinoma (NPC). However, in gastric cancer (GC) the role of
BPIFB1
is not known, but the expression of the gene has been reported in the stomach co-expressing E-cadherin (
CDH1
) together with making mucin 5, tracheobronchial/gastric mucin type A and C (
MUC5AC
). To investigate the role of
BPIFB1
in GC development and its interactions with
CDH1
and
MUC5AC
, overexpression of the gene was induced in three different GC cell lines; AGS, HGC-27 and MKN-45. The present of
BPIFB1
protein in a transfected GC cell line was detected through gel Lumio Green Detection.
BPIFB1
,
CDH1
and
MUC5AC
expression were then performed via RT-PCR and quantitative Real-time PCR (qPCR) followed by statistical analysis (
p
≤ 0.05).
BPIFB1
expression was detected in all transfected cells with the highest in AGS with the
p
= 0.003 followed by MKN-45 with the
p
= 0.009 and HGC-27 with the
p
= 0.015. The expression of
MUC5AC
is upregulated in
BPIFB1
transfected GC cells whereby the highest expression is observed in
MKN-45
with the
p
= 0.05 followed by AGS with the
p
= 0.001 and HGC-27 with the
p
= 0.026.
CDH1
expression is downregulated in all
BPIFB1
transfected GC cells with the lowest expression found in HGC-27 with the
p
= 0.001 followed by AGS with the
p
= 0.003 and MKN-45 with the
p
= 0.004. Thus, induction of
BPIFB1
overexpression in vitro in GC cells contributes to the decreased in
CDH1
expression and increased in
MUC5AC
expression suggesting that
BPIFB1
other than co-expressed in the gastric gland with the two genes also regulates gene expression. We found that the overexpression of
BPIFB1
downregulated the expression of
CDH1
and upregulated
MUC5AC
gene expression. This finding was supported by both RT-PCR and qPCR. Conclusion: Our findings suggest that
BPIFB1
is differentially expressed in GC cell lines and regulate the expression of
CDH1
and
MUC5AC
.</description><identifier>ISSN: 1062-3590</identifier><identifier>EISSN: 1608-3059</identifier><identifier>DOI: 10.1134/S1062359022601756</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; bulls ; cadherins ; carcinoma ; Cell Biology ; cell lines ; Down-regulation ; E-cadherin ; Ecology ; Gastric cancer ; Gastric glands ; gels ; Gene expression ; gene expression regulation ; genes ; humans ; Life Sciences ; Mucin ; mucins ; Nasopharyngeal carcinoma ; Permeability ; Polymerase chain reaction ; quantitative polymerase chain reaction ; Statistical analysis ; stomach ; stomach neoplasms ; Zoology</subject><ispartof>Biology bulletin of the Russian Academy of Sciences, 2023-08, Vol.50 (4), p.532-545</ispartof><rights>Pleiades Publishing, Inc. 2023. ISSN 1062-3590, Biology Bulletin, 2023, Vol. 50, No. 4, pp. 532–545. © Pleiades Publishing, Inc., 2023. ISSN 1062-3590, Biology Bulletin, 2023. © Pleiades Publishing, Inc., 2023.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c301t-d195ea6a70e1f35410afdb21a05e409d6bbc7d48f8b55dce49635a92d18bebb23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1062359022601756$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1062359022601756$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids></links><search><creatorcontrib>Nor Azlin Safina Abdul Aziz</creatorcontrib><creatorcontrib>Musa, Maslinda</creatorcontrib><creatorcontrib>Kadir, Siti Hamimah Sheikh Abdul</creatorcontrib><creatorcontrib>Karim, Zeti Rahayu Abdul</creatorcontrib><creatorcontrib>Hasani, Narimah Abdul Hamid</creatorcontrib><title>Overexpression of Bactericidal/Permeability-Increasing Fold-Containing Family BPIFB1 in Gastric Cancer Cells Leads to Differential Expression of E-Cadherin and MUC5AC</title><title>Biology bulletin of the Russian Academy of Sciences</title><addtitle>Biol Bull Russ Acad Sci</addtitle><description>The human Bactericidal/Permeability-Increasing Fold containing family B, member 1 (BPIFB1), or also known as LPLUNC1, C20orf114, is a member of the BPI/LBP/PLUNC protein family.
BPIFB1
differential expression patterns have been reported in several cancers such as nasopharyngeal carcinoma (NPC). However, in gastric cancer (GC) the role of
BPIFB1
is not known, but the expression of the gene has been reported in the stomach co-expressing E-cadherin (
CDH1
) together with making mucin 5, tracheobronchial/gastric mucin type A and C (
MUC5AC
). To investigate the role of
BPIFB1
in GC development and its interactions with
CDH1
and
MUC5AC
, overexpression of the gene was induced in three different GC cell lines; AGS, HGC-27 and MKN-45. The present of
BPIFB1
protein in a transfected GC cell line was detected through gel Lumio Green Detection.
BPIFB1
,
CDH1
and
MUC5AC
expression were then performed via RT-PCR and quantitative Real-time PCR (qPCR) followed by statistical analysis (
p
≤ 0.05).
BPIFB1
expression was detected in all transfected cells with the highest in AGS with the
p
= 0.003 followed by MKN-45 with the
p
= 0.009 and HGC-27 with the
p
= 0.015. The expression of
MUC5AC
is upregulated in
BPIFB1
transfected GC cells whereby the highest expression is observed in
MKN-45
with the
p
= 0.05 followed by AGS with the
p
= 0.001 and HGC-27 with the
p
= 0.026.
CDH1
expression is downregulated in all
BPIFB1
transfected GC cells with the lowest expression found in HGC-27 with the
p
= 0.001 followed by AGS with the
p
= 0.003 and MKN-45 with the
p
= 0.004. Thus, induction of
BPIFB1
overexpression in vitro in GC cells contributes to the decreased in
CDH1
expression and increased in
MUC5AC
expression suggesting that
BPIFB1
other than co-expressed in the gastric gland with the two genes also regulates gene expression. We found that the overexpression of
BPIFB1
downregulated the expression of
CDH1
and upregulated
MUC5AC
gene expression. This finding was supported by both RT-PCR and qPCR. Conclusion: Our findings suggest that
BPIFB1
is differentially expressed in GC cell lines and regulate the expression of
CDH1
and
MUC5AC
.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>bulls</subject><subject>cadherins</subject><subject>carcinoma</subject><subject>Cell Biology</subject><subject>cell lines</subject><subject>Down-regulation</subject><subject>E-cadherin</subject><subject>Ecology</subject><subject>Gastric cancer</subject><subject>Gastric glands</subject><subject>gels</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>genes</subject><subject>humans</subject><subject>Life Sciences</subject><subject>Mucin</subject><subject>mucins</subject><subject>Nasopharyngeal carcinoma</subject><subject>Permeability</subject><subject>Polymerase chain reaction</subject><subject>quantitative polymerase chain reaction</subject><subject>Statistical analysis</subject><subject>stomach</subject><subject>stomach neoplasms</subject><subject>Zoology</subject><issn>1062-3590</issn><issn>1608-3059</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kc9O3DAQxqOKSuXfA_RmqZdeUjx2nE2ObNiFlbYCqXCOJvaEGmXtxc6i7gvxnHhZJNRWnMaj-X3ffPJk2VfgPwBkcfYLeCmkqrkQJYeJKj9lh1DyKpdc1Qfpncb5bv4lO4rxgXNeyEIcZs_XTxTozzpQjNY75ns2RT1SsNoaHM5uKKwIOzvYcZsvnA6E0bp7NveDyRvvRrTutceVHbZserOYT4FZxy4xjsmENeg0BdbQMES2JDSRjZ5d2L5Pe91ocWCzv9bP8gbN7xTAMXSG_bxr1Hlzkn3ucYh0-laPs7v57La5ypfXl4vmfJlryWHMDdSKsMQJJ-ilKoBjbzoByBUVvDZl1-mJKaq-6pQymoq6lAprYaDqqOuEPM6-733XwT9uKI7tykadsqMjv4mtBCV331rLhH77B33wm-BSulZUCqCeKFEmCvaUDj7GQH27DnaFYdsCb3eXa_-7XNKIvSYm1t1TeHf-WPQC6E6bLw</recordid><startdate>20230801</startdate><enddate>20230801</enddate><creator>Nor Azlin Safina Abdul Aziz</creator><creator>Musa, Maslinda</creator><creator>Kadir, Siti Hamimah Sheikh Abdul</creator><creator>Karim, Zeti Rahayu Abdul</creator><creator>Hasani, Narimah Abdul Hamid</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20230801</creationdate><title>Overexpression of Bactericidal/Permeability-Increasing Fold-Containing Family BPIFB1 in Gastric Cancer Cells Leads to Differential Expression of E-Cadherin and MUC5AC</title><author>Nor Azlin Safina Abdul Aziz ; Musa, Maslinda ; Kadir, Siti Hamimah Sheikh Abdul ; Karim, Zeti Rahayu Abdul ; Hasani, Narimah Abdul Hamid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-d195ea6a70e1f35410afdb21a05e409d6bbc7d48f8b55dce49635a92d18bebb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>bulls</topic><topic>cadherins</topic><topic>carcinoma</topic><topic>Cell Biology</topic><topic>cell lines</topic><topic>Down-regulation</topic><topic>E-cadherin</topic><topic>Ecology</topic><topic>Gastric cancer</topic><topic>Gastric glands</topic><topic>gels</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>genes</topic><topic>humans</topic><topic>Life Sciences</topic><topic>Mucin</topic><topic>mucins</topic><topic>Nasopharyngeal carcinoma</topic><topic>Permeability</topic><topic>Polymerase chain reaction</topic><topic>quantitative polymerase chain reaction</topic><topic>Statistical analysis</topic><topic>stomach</topic><topic>stomach neoplasms</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nor Azlin Safina Abdul Aziz</creatorcontrib><creatorcontrib>Musa, Maslinda</creatorcontrib><creatorcontrib>Kadir, Siti Hamimah Sheikh Abdul</creatorcontrib><creatorcontrib>Karim, Zeti Rahayu Abdul</creatorcontrib><creatorcontrib>Hasani, Narimah Abdul Hamid</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Biology bulletin of the Russian Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nor Azlin Safina Abdul Aziz</au><au>Musa, Maslinda</au><au>Kadir, Siti Hamimah Sheikh Abdul</au><au>Karim, Zeti Rahayu Abdul</au><au>Hasani, Narimah Abdul Hamid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of Bactericidal/Permeability-Increasing Fold-Containing Family BPIFB1 in Gastric Cancer Cells Leads to Differential Expression of E-Cadherin and MUC5AC</atitle><jtitle>Biology bulletin of the Russian Academy of Sciences</jtitle><stitle>Biol Bull Russ Acad Sci</stitle><date>2023-08-01</date><risdate>2023</risdate><volume>50</volume><issue>4</issue><spage>532</spage><epage>545</epage><pages>532-545</pages><issn>1062-3590</issn><eissn>1608-3059</eissn><abstract>The human Bactericidal/Permeability-Increasing Fold containing family B, member 1 (BPIFB1), or also known as LPLUNC1, C20orf114, is a member of the BPI/LBP/PLUNC protein family.
BPIFB1
differential expression patterns have been reported in several cancers such as nasopharyngeal carcinoma (NPC). However, in gastric cancer (GC) the role of
BPIFB1
is not known, but the expression of the gene has been reported in the stomach co-expressing E-cadherin (
CDH1
) together with making mucin 5, tracheobronchial/gastric mucin type A and C (
MUC5AC
). To investigate the role of
BPIFB1
in GC development and its interactions with
CDH1
and
MUC5AC
, overexpression of the gene was induced in three different GC cell lines; AGS, HGC-27 and MKN-45. The present of
BPIFB1
protein in a transfected GC cell line was detected through gel Lumio Green Detection.
BPIFB1
,
CDH1
and
MUC5AC
expression were then performed via RT-PCR and quantitative Real-time PCR (qPCR) followed by statistical analysis (
p
≤ 0.05).
BPIFB1
expression was detected in all transfected cells with the highest in AGS with the
p
= 0.003 followed by MKN-45 with the
p
= 0.009 and HGC-27 with the
p
= 0.015. The expression of
MUC5AC
is upregulated in
BPIFB1
transfected GC cells whereby the highest expression is observed in
MKN-45
with the
p
= 0.05 followed by AGS with the
p
= 0.001 and HGC-27 with the
p
= 0.026.
CDH1
expression is downregulated in all
BPIFB1
transfected GC cells with the lowest expression found in HGC-27 with the
p
= 0.001 followed by AGS with the
p
= 0.003 and MKN-45 with the
p
= 0.004. Thus, induction of
BPIFB1
overexpression in vitro in GC cells contributes to the decreased in
CDH1
expression and increased in
MUC5AC
expression suggesting that
BPIFB1
other than co-expressed in the gastric gland with the two genes also regulates gene expression. We found that the overexpression of
BPIFB1
downregulated the expression of
CDH1
and upregulated
MUC5AC
gene expression. This finding was supported by both RT-PCR and qPCR. Conclusion: Our findings suggest that
BPIFB1
is differentially expressed in GC cell lines and regulate the expression of
CDH1
and
MUC5AC
.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1062359022601756</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1062-3590 |
| ispartof | Biology bulletin of the Russian Academy of Sciences, 2023-08, Vol.50 (4), p.532-545 |
| issn | 1062-3590 1608-3059 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Biochemistry Biomedical and Life Sciences bulls cadherins carcinoma Cell Biology cell lines Down-regulation E-cadherin Ecology Gastric cancer Gastric glands gels Gene expression gene expression regulation genes humans Life Sciences Mucin mucins Nasopharyngeal carcinoma Permeability Polymerase chain reaction quantitative polymerase chain reaction Statistical analysis stomach stomach neoplasms Zoology |
| title | Overexpression of Bactericidal/Permeability-Increasing Fold-Containing Family BPIFB1 in Gastric Cancer Cells Leads to Differential Expression of E-Cadherin and MUC5AC |
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