A comprehensive study of Ephedra sinica Stapf-Schisandra chinensis (Turcz.) Baill herb pair on airway protection in asthma
Ephedra sinica Stapf (Mahuang) and Schisandra chinensis (Turcz.) Baill (Wuweizi) are commonly utilized in traditional Chinese medicine for the treatment of cough and asthma. The synergistic effect of Mahuang-Wuweizi herb pair enhances their efficacy in alleviating respiratory symptoms, making them e...
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| Veröffentlicht in: | Journal of ethnopharmacology 2024-03, Vol.322, p.117614-117614, Article 117614 |
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| creator | Zhuo, Zushun Nie, Jianhua Xie, Bin Wang, Fei Shi, Min Jiang, Yini Zhu, Weifeng |
| description | Ephedra sinica Stapf (Mahuang) and Schisandra chinensis (Turcz.) Baill (Wuweizi) are commonly utilized in traditional Chinese medicine for the treatment of cough and asthma. The synergistic effect of Mahuang-Wuweizi herb pair enhances their efficacy in alleviating respiratory symptoms, making them extensively employed in the management of respiratory disorders. Although previous studies have demonstrated the therapeutic potential of Mahuang-Wuweizi in pulmonary fibrosis, the precise mechanism underlying their effectiveness against asthma remains elusive.
The objective of this study is to investigate the mechanism underlying the preventive and therapeutic effects of Mahuang-Wuweizi herb pair on asthma progression, focusing on airway inflammation and airway remodeling.
The active constituents and potential mechanisms of Mahuang-Wuweizi in the management of asthma were elucidated through network pharmacology analysis. Liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to detect the main components of Mahuang-Wuweizi decoction. A rat model of bronchial asthma was established, and the effects of Mahuang-Wuweizi were investigated using hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) staining, enzyme-linked immunosorbent assay (ELISA), Western blotting (WB), and real-time reverse transcription polymerase chain reaction (RT-qPCR).
The results of network pharmacological prediction showed that Mahuang had 22 active components and Wuweizi had 8 active components, with 225 potential targets. 1159 targets associated with asthma and 115 targets that overlap between drugs and diseases were identified. These include interleukin-6 (IL-6), tumor necrosis factor (TNF), Tumor Protein 53, interleukin-1β (IL-1β), as well as other essential targets. Additionally, there is a potential correlation between asthma and Phosphatidylinositol 3 kinase (PI3K)/Protein Kinase B (AKT) signaling pathway, calcium ion channels, nuclear factor-kappa B (NF-κB) signaling pathway, and other signaling pathways. The animal experiment results demonstrated that treatment with Mahuang and Wuweizi, in comparison to the model group, exhibited improvements in lung tissue pathological injury, reduction in collagen fiber accumulation around the airway and proliferation of airway smooth muscle, decrease in concentration levels of IL-6, TNF-α and IL-1β in lung tissue, as well as alleviation of airway inflammation. Furthermore, Mahuang and Wuweizi suppressed the expression of phosph |
| doi_str_mv | 10.1016/j.jep.2023.117614 |
| format | Article |
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The objective of this study is to investigate the mechanism underlying the preventive and therapeutic effects of Mahuang-Wuweizi herb pair on asthma progression, focusing on airway inflammation and airway remodeling.
The active constituents and potential mechanisms of Mahuang-Wuweizi in the management of asthma were elucidated through network pharmacology analysis. Liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to detect the main components of Mahuang-Wuweizi decoction. A rat model of bronchial asthma was established, and the effects of Mahuang-Wuweizi were investigated using hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) staining, enzyme-linked immunosorbent assay (ELISA), Western blotting (WB), and real-time reverse transcription polymerase chain reaction (RT-qPCR).
The results of network pharmacological prediction showed that Mahuang had 22 active components and Wuweizi had 8 active components, with 225 potential targets. 1159 targets associated with asthma and 115 targets that overlap between drugs and diseases were identified. These include interleukin-6 (IL-6), tumor necrosis factor (TNF), Tumor Protein 53, interleukin-1β (IL-1β), as well as other essential targets. Additionally, there is a potential correlation between asthma and Phosphatidylinositol 3 kinase (PI3K)/Protein Kinase B (AKT) signaling pathway, calcium ion channels, nuclear factor-kappa B (NF-κB) signaling pathway, and other signaling pathways. The animal experiment results demonstrated that treatment with Mahuang and Wuweizi, in comparison to the model group, exhibited improvements in lung tissue pathological injury, reduction in collagen fiber accumulation around the airway and proliferation of airway smooth muscle, decrease in concentration levels of IL-6, TNF-α and IL-1β in lung tissue, as well as alleviation of airway inflammation. Furthermore, Mahuang and Wuweizi suppressed the expression of phospholipase C (PLC), transient receptor potential channel 1 (TRPC1), myosin light chain kinase (MLCK), NF-κB P65 protein in ovalbumin (OVA)-sensitized rat lung tissue and downregulated the mRNA expression of PLC, TRPC1, PI3K, AKT, NF-κB P65 in asthmatic rats. These findings were consistent with network pharmacological analysis.
The results show that the synergistic interaction between Mahuang and Wuweizi occur, and they can effectively reduce airway remodeling and airway inflammation induced by inhaling OVA in bronchial asthma rats by inhibiting the expression of PLC/TRPC1/PI3K/AKT/NF-κB signaling pathway. Therefore, Mahuang and Wuweizi may be potential drugs to treat asthma.
[Display omitted]
•Mahuang and Wuweizi herb pair have the potential to treat asthma.•PLC/TRPC1/PI3K/AKT/NF-κB signaling pathway is involved in airway injury in asthma.•Mahuang and Wuweizi can alleviate asthma by regulating the PLC/TRPC1/PI3K/AKT/NF-κB signaling pathway.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2023.117614</identifier><identifier>PMID: 38113990</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>animal experimentation ; animal models ; asthma ; calcium ; collagen ; cough ; enzyme-linked immunosorbent assay ; Ephedra sinica ; Ephedra sinica Stapf ; gene expression ; immunohistochemistry ; inflammation ; interleukin-6 ; liquid chromatography ; lungs ; myosin light chain kinase ; neoplasms ; Network pharmacology ; NF-κB ; Oriental traditional medicine ; ovalbumin ; pharmacology ; phosphatidylinositol 3-kinase ; phospholipase C ; prediction ; pulmonary fibrosis ; rats ; reverse transcriptase polymerase chain reaction ; Schisandra chinensis ; Schisandra chinensis (Turcz.) Baill ; smooth muscle ; synergism ; tandem mass spectrometry ; therapeutics ; transcription factor NF-kappa B ; transient receptor potential channels ; TRPC1 ; tumor necrosis factors</subject><ispartof>Journal of ethnopharmacology, 2024-03, Vol.322, p.117614-117614, Article 117614</ispartof><rights>2023 Elsevier B.V.</rights><rights>Copyright © 2023. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c338t-f6452ee81880c4060571fd734ea743eea1931767e7dc55184c2d1904b57fce093</cites><orcidid>0009-0003-9097-5783</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378874123014848$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38113990$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhuo, Zushun</creatorcontrib><creatorcontrib>Nie, Jianhua</creatorcontrib><creatorcontrib>Xie, Bin</creatorcontrib><creatorcontrib>Wang, Fei</creatorcontrib><creatorcontrib>Shi, Min</creatorcontrib><creatorcontrib>Jiang, Yini</creatorcontrib><creatorcontrib>Zhu, Weifeng</creatorcontrib><title>A comprehensive study of Ephedra sinica Stapf-Schisandra chinensis (Turcz.) Baill herb pair on airway protection in asthma</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Ephedra sinica Stapf (Mahuang) and Schisandra chinensis (Turcz.) Baill (Wuweizi) are commonly utilized in traditional Chinese medicine for the treatment of cough and asthma. The synergistic effect of Mahuang-Wuweizi herb pair enhances their efficacy in alleviating respiratory symptoms, making them extensively employed in the management of respiratory disorders. Although previous studies have demonstrated the therapeutic potential of Mahuang-Wuweizi in pulmonary fibrosis, the precise mechanism underlying their effectiveness against asthma remains elusive.
The objective of this study is to investigate the mechanism underlying the preventive and therapeutic effects of Mahuang-Wuweizi herb pair on asthma progression, focusing on airway inflammation and airway remodeling.
The active constituents and potential mechanisms of Mahuang-Wuweizi in the management of asthma were elucidated through network pharmacology analysis. Liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to detect the main components of Mahuang-Wuweizi decoction. A rat model of bronchial asthma was established, and the effects of Mahuang-Wuweizi were investigated using hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) staining, enzyme-linked immunosorbent assay (ELISA), Western blotting (WB), and real-time reverse transcription polymerase chain reaction (RT-qPCR).
The results of network pharmacological prediction showed that Mahuang had 22 active components and Wuweizi had 8 active components, with 225 potential targets. 1159 targets associated with asthma and 115 targets that overlap between drugs and diseases were identified. These include interleukin-6 (IL-6), tumor necrosis factor (TNF), Tumor Protein 53, interleukin-1β (IL-1β), as well as other essential targets. Additionally, there is a potential correlation between asthma and Phosphatidylinositol 3 kinase (PI3K)/Protein Kinase B (AKT) signaling pathway, calcium ion channels, nuclear factor-kappa B (NF-κB) signaling pathway, and other signaling pathways. The animal experiment results demonstrated that treatment with Mahuang and Wuweizi, in comparison to the model group, exhibited improvements in lung tissue pathological injury, reduction in collagen fiber accumulation around the airway and proliferation of airway smooth muscle, decrease in concentration levels of IL-6, TNF-α and IL-1β in lung tissue, as well as alleviation of airway inflammation. Furthermore, Mahuang and Wuweizi suppressed the expression of phospholipase C (PLC), transient receptor potential channel 1 (TRPC1), myosin light chain kinase (MLCK), NF-κB P65 protein in ovalbumin (OVA)-sensitized rat lung tissue and downregulated the mRNA expression of PLC, TRPC1, PI3K, AKT, NF-κB P65 in asthmatic rats. These findings were consistent with network pharmacological analysis.
The results show that the synergistic interaction between Mahuang and Wuweizi occur, and they can effectively reduce airway remodeling and airway inflammation induced by inhaling OVA in bronchial asthma rats by inhibiting the expression of PLC/TRPC1/PI3K/AKT/NF-κB signaling pathway. Therefore, Mahuang and Wuweizi may be potential drugs to treat asthma.
[Display omitted]
•Mahuang and Wuweizi herb pair have the potential to treat asthma.•PLC/TRPC1/PI3K/AKT/NF-κB signaling pathway is involved in airway injury in asthma.•Mahuang and Wuweizi can alleviate asthma by regulating the PLC/TRPC1/PI3K/AKT/NF-κB signaling pathway.</description><subject>animal experimentation</subject><subject>animal models</subject><subject>asthma</subject><subject>calcium</subject><subject>collagen</subject><subject>cough</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Ephedra sinica</subject><subject>Ephedra sinica Stapf</subject><subject>gene expression</subject><subject>immunohistochemistry</subject><subject>inflammation</subject><subject>interleukin-6</subject><subject>liquid chromatography</subject><subject>lungs</subject><subject>myosin light chain kinase</subject><subject>neoplasms</subject><subject>Network pharmacology</subject><subject>NF-κB</subject><subject>Oriental traditional medicine</subject><subject>ovalbumin</subject><subject>pharmacology</subject><subject>phosphatidylinositol 3-kinase</subject><subject>phospholipase C</subject><subject>prediction</subject><subject>pulmonary fibrosis</subject><subject>rats</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Schisandra chinensis</subject><subject>Schisandra chinensis (Turcz.) Baill</subject><subject>smooth muscle</subject><subject>synergism</subject><subject>tandem mass spectrometry</subject><subject>therapeutics</subject><subject>transcription factor NF-kappa B</subject><subject>transient receptor potential channels</subject><subject>TRPC1</subject><subject>tumor necrosis factors</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc1q3DAUhUVpaCaTPkA3RctkYUfXki2ZrqZD0gYCWSRZC418jTX4r5I9ZfL0kZm0yxYEVxy-c5DuIeQLsBQYFDf7dI9jmrGMpwCyAPGBrEDJLJG55B_JinGpEiUFnJOLEPaMMQmCfSLnXAHwsmQr8rqhduhGjw32wR2QhmmujnSo6e3YYOUNDa531tCnyYx18mQbF0y_6PHWL55Ar55nb1_Ta_rduLalDfodHY3zdOhpHL_NkY5-mNBOLiouimFqOnNJzmrTBvz8Ptfk5e72efszeXj8cb_dPCSWczUldSHyDFGBUswKVrBcQl1JLtBIwRENlDx-XqKsbJ6DEjaroGRil8vaIiv5mlydcuMjfs0YJt25YLFtTY_DHDSHPJ6y4PBfNIu5kVZiQeGEWj-E4LHWo3ed8UcNTC_t6L2O7eilHX1qJ3q-vsfPuw6rv44_dUTg2wnAuI-DQ6-DddhbrJyP69PV4P4R_waA658S</recordid><startdate>20240325</startdate><enddate>20240325</enddate><creator>Zhuo, Zushun</creator><creator>Nie, Jianhua</creator><creator>Xie, Bin</creator><creator>Wang, Fei</creator><creator>Shi, Min</creator><creator>Jiang, Yini</creator><creator>Zhu, Weifeng</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0009-0003-9097-5783</orcidid></search><sort><creationdate>20240325</creationdate><title>A comprehensive study of Ephedra sinica Stapf-Schisandra chinensis (Turcz.) Baill herb pair on airway protection in asthma</title><author>Zhuo, Zushun ; Nie, Jianhua ; Xie, Bin ; Wang, Fei ; Shi, Min ; Jiang, Yini ; Zhu, Weifeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-f6452ee81880c4060571fd734ea743eea1931767e7dc55184c2d1904b57fce093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>animal experimentation</topic><topic>animal models</topic><topic>asthma</topic><topic>calcium</topic><topic>collagen</topic><topic>cough</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Ephedra sinica</topic><topic>Ephedra sinica Stapf</topic><topic>gene expression</topic><topic>immunohistochemistry</topic><topic>inflammation</topic><topic>interleukin-6</topic><topic>liquid chromatography</topic><topic>lungs</topic><topic>myosin light chain kinase</topic><topic>neoplasms</topic><topic>Network pharmacology</topic><topic>NF-κB</topic><topic>Oriental traditional medicine</topic><topic>ovalbumin</topic><topic>pharmacology</topic><topic>phosphatidylinositol 3-kinase</topic><topic>phospholipase C</topic><topic>prediction</topic><topic>pulmonary fibrosis</topic><topic>rats</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>Schisandra chinensis</topic><topic>Schisandra chinensis (Turcz.) Baill</topic><topic>smooth muscle</topic><topic>synergism</topic><topic>tandem mass spectrometry</topic><topic>therapeutics</topic><topic>transcription factor NF-kappa B</topic><topic>transient receptor potential channels</topic><topic>TRPC1</topic><topic>tumor necrosis factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhuo, Zushun</creatorcontrib><creatorcontrib>Nie, Jianhua</creatorcontrib><creatorcontrib>Xie, Bin</creatorcontrib><creatorcontrib>Wang, Fei</creatorcontrib><creatorcontrib>Shi, Min</creatorcontrib><creatorcontrib>Jiang, Yini</creatorcontrib><creatorcontrib>Zhu, Weifeng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhuo, Zushun</au><au>Nie, Jianhua</au><au>Xie, Bin</au><au>Wang, Fei</au><au>Shi, Min</au><au>Jiang, Yini</au><au>Zhu, Weifeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comprehensive study of Ephedra sinica Stapf-Schisandra chinensis (Turcz.) Baill herb pair on airway protection in asthma</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2024-03-25</date><risdate>2024</risdate><volume>322</volume><spage>117614</spage><epage>117614</epage><pages>117614-117614</pages><artnum>117614</artnum><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Ephedra sinica Stapf (Mahuang) and Schisandra chinensis (Turcz.) Baill (Wuweizi) are commonly utilized in traditional Chinese medicine for the treatment of cough and asthma. The synergistic effect of Mahuang-Wuweizi herb pair enhances their efficacy in alleviating respiratory symptoms, making them extensively employed in the management of respiratory disorders. Although previous studies have demonstrated the therapeutic potential of Mahuang-Wuweizi in pulmonary fibrosis, the precise mechanism underlying their effectiveness against asthma remains elusive.
The objective of this study is to investigate the mechanism underlying the preventive and therapeutic effects of Mahuang-Wuweizi herb pair on asthma progression, focusing on airway inflammation and airway remodeling.
The active constituents and potential mechanisms of Mahuang-Wuweizi in the management of asthma were elucidated through network pharmacology analysis. Liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to detect the main components of Mahuang-Wuweizi decoction. A rat model of bronchial asthma was established, and the effects of Mahuang-Wuweizi were investigated using hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) staining, enzyme-linked immunosorbent assay (ELISA), Western blotting (WB), and real-time reverse transcription polymerase chain reaction (RT-qPCR).
The results of network pharmacological prediction showed that Mahuang had 22 active components and Wuweizi had 8 active components, with 225 potential targets. 1159 targets associated with asthma and 115 targets that overlap between drugs and diseases were identified. These include interleukin-6 (IL-6), tumor necrosis factor (TNF), Tumor Protein 53, interleukin-1β (IL-1β), as well as other essential targets. Additionally, there is a potential correlation between asthma and Phosphatidylinositol 3 kinase (PI3K)/Protein Kinase B (AKT) signaling pathway, calcium ion channels, nuclear factor-kappa B (NF-κB) signaling pathway, and other signaling pathways. The animal experiment results demonstrated that treatment with Mahuang and Wuweizi, in comparison to the model group, exhibited improvements in lung tissue pathological injury, reduction in collagen fiber accumulation around the airway and proliferation of airway smooth muscle, decrease in concentration levels of IL-6, TNF-α and IL-1β in lung tissue, as well as alleviation of airway inflammation. Furthermore, Mahuang and Wuweizi suppressed the expression of phospholipase C (PLC), transient receptor potential channel 1 (TRPC1), myosin light chain kinase (MLCK), NF-κB P65 protein in ovalbumin (OVA)-sensitized rat lung tissue and downregulated the mRNA expression of PLC, TRPC1, PI3K, AKT, NF-κB P65 in asthmatic rats. These findings were consistent with network pharmacological analysis.
The results show that the synergistic interaction between Mahuang and Wuweizi occur, and they can effectively reduce airway remodeling and airway inflammation induced by inhaling OVA in bronchial asthma rats by inhibiting the expression of PLC/TRPC1/PI3K/AKT/NF-κB signaling pathway. Therefore, Mahuang and Wuweizi may be potential drugs to treat asthma.
[Display omitted]
•Mahuang and Wuweizi herb pair have the potential to treat asthma.•PLC/TRPC1/PI3K/AKT/NF-κB signaling pathway is involved in airway injury in asthma.•Mahuang and Wuweizi can alleviate asthma by regulating the PLC/TRPC1/PI3K/AKT/NF-κB signaling pathway.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>38113990</pmid><doi>10.1016/j.jep.2023.117614</doi><tpages>1</tpages><orcidid>https://orcid.org/0009-0003-9097-5783</orcidid></addata></record> |
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| subjects | animal experimentation animal models asthma calcium collagen cough enzyme-linked immunosorbent assay Ephedra sinica Ephedra sinica Stapf gene expression immunohistochemistry inflammation interleukin-6 liquid chromatography lungs myosin light chain kinase neoplasms Network pharmacology NF-κB Oriental traditional medicine ovalbumin pharmacology phosphatidylinositol 3-kinase phospholipase C prediction pulmonary fibrosis rats reverse transcriptase polymerase chain reaction Schisandra chinensis Schisandra chinensis (Turcz.) Baill smooth muscle synergism tandem mass spectrometry therapeutics transcription factor NF-kappa B transient receptor potential channels TRPC1 tumor necrosis factors |
| title | A comprehensive study of Ephedra sinica Stapf-Schisandra chinensis (Turcz.) Baill herb pair on airway protection in asthma |
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