LC-HRMS and FTIR-based metabolomics analysis and xanthine oxidase inhibitory evaluation of Sida rhombifolia with different drying methods
Sida rhombifolia, known as Sidaguri in Indonesia, has been widely used in traditional medicine because of its bioactive metabolites, particularly flavonoids and steroids. Several factors, including the drying method, can affect the composition and concentrations of metabolites in plants. This study...
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| Veröffentlicht in: | Biocatalysis and agricultural biotechnology 2023-09, Vol.52, p.102833, Article 102833 |
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| creator | Silviani, Dea Wahyuni, Wulan Tri Syafitri, Utami Dyah Ilmiawati, Auliya Septaningsih, Dewi Anggraini Insanu, Muhamad Aminah, Nanik Siti Rohman, Abdul Rafi, Mohamad |
| description | Sida rhombifolia, known as Sidaguri in Indonesia, has been widely used in traditional medicine because of its bioactive metabolites, particularly flavonoids and steroids. Several factors, including the drying method, can affect the composition and concentrations of metabolites in plants. This study aimed to profile the metabolites and evaluate the xanthine oxidase inhibitory activity of S. rhombifolia dried by different methods. Three drying methods were used: air drying (AD), oven drying (OD), and sun drying (SD). Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and Fourier transform infrared (FTIR)-based untargeted metabolomics were used to profile the metabolites in S. rhombifolia. The FTIR spectra of samples dried by different methods showed similar patterns, with peaks at 3398, 2935, 2856, 1728, 1456, and 1164 cm−1. Metabolite profiling of S. rhombifolia using liquid chromatography–tandem mass spectrometry (LC–MS/MS) identified 24 putative metabolites, with 20, 15, and 16 metabolites found in the AD, OD, and SD extracts, respectively. The principal component analysis demonstrated the separation of S. rhombifolia into groups based on drying method differences. These differences account for 95% (LC–MS/MS) and 99% (FTIR) of the total variance explained. Evaluation of xanthine oxidase inhibition revealed that S. rhombifolia inhibited the enzyme by 22.81% (AD), 35.97% (OD), and 45.93% (SD) at an extract concentration of 50 ppm. The drying method affected the metabolite profile with regard to both the functional groups and amounts of metabolites. Furthermore, the results showed that the xanthine oxidase inhibitory activity depended on the S. rhombifolia drying method and conditions.
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•Different drying methods can affect the metabolite profile in Sida rhombifolia.•Combined FTIR and LC-MS/MS•Using chemometrics for data analysis•Putative metabolite identification by MS2 spectra |
| doi_str_mv | 10.1016/j.bcab.2023.102833 |
| format | Article |
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•Different drying methods can affect the metabolite profile in Sida rhombifolia.•Combined FTIR and LC-MS/MS•Using chemometrics for data analysis•Putative metabolite identification by MS2 spectra</description><identifier>ISSN: 1878-8181</identifier><identifier>EISSN: 1878-8181</identifier><identifier>DOI: 10.1016/j.bcab.2023.102833</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>agricultural biotechnology ; air ; biocatalysis ; Drying method ; flavonoids ; Fourier transform infrared spectroscopy ; FTIR spectra ; Indonesia ; LC–MS/MS ; liquid chromatography ; metabolites ; Metabolomics ; ovens ; principal component analysis ; Sida rhombifolia ; tandem mass spectrometry ; traditional medicine ; variance ; Xanthine oxidase</subject><ispartof>Biocatalysis and agricultural biotechnology, 2023-09, Vol.52, p.102833, Article 102833</ispartof><rights>2023 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c284t-bf743d2b98ce0b1b3c877ac522b40b7ea0fb2a4f67124bedfbf8a31efb0d9fd73</cites><orcidid>0000-0002-5225-8703</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Silviani, Dea</creatorcontrib><creatorcontrib>Wahyuni, Wulan Tri</creatorcontrib><creatorcontrib>Syafitri, Utami Dyah</creatorcontrib><creatorcontrib>Ilmiawati, Auliya</creatorcontrib><creatorcontrib>Septaningsih, Dewi Anggraini</creatorcontrib><creatorcontrib>Insanu, Muhamad</creatorcontrib><creatorcontrib>Aminah, Nanik Siti</creatorcontrib><creatorcontrib>Rohman, Abdul</creatorcontrib><creatorcontrib>Rafi, Mohamad</creatorcontrib><title>LC-HRMS and FTIR-based metabolomics analysis and xanthine oxidase inhibitory evaluation of Sida rhombifolia with different drying methods</title><title>Biocatalysis and agricultural biotechnology</title><description>Sida rhombifolia, known as Sidaguri in Indonesia, has been widely used in traditional medicine because of its bioactive metabolites, particularly flavonoids and steroids. Several factors, including the drying method, can affect the composition and concentrations of metabolites in plants. This study aimed to profile the metabolites and evaluate the xanthine oxidase inhibitory activity of S. rhombifolia dried by different methods. Three drying methods were used: air drying (AD), oven drying (OD), and sun drying (SD). Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and Fourier transform infrared (FTIR)-based untargeted metabolomics were used to profile the metabolites in S. rhombifolia. The FTIR spectra of samples dried by different methods showed similar patterns, with peaks at 3398, 2935, 2856, 1728, 1456, and 1164 cm−1. Metabolite profiling of S. rhombifolia using liquid chromatography–tandem mass spectrometry (LC–MS/MS) identified 24 putative metabolites, with 20, 15, and 16 metabolites found in the AD, OD, and SD extracts, respectively. The principal component analysis demonstrated the separation of S. rhombifolia into groups based on drying method differences. These differences account for 95% (LC–MS/MS) and 99% (FTIR) of the total variance explained. Evaluation of xanthine oxidase inhibition revealed that S. rhombifolia inhibited the enzyme by 22.81% (AD), 35.97% (OD), and 45.93% (SD) at an extract concentration of 50 ppm. The drying method affected the metabolite profile with regard to both the functional groups and amounts of metabolites. Furthermore, the results showed that the xanthine oxidase inhibitory activity depended on the S. rhombifolia drying method and conditions.
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•Different drying methods can affect the metabolite profile in Sida rhombifolia.•Combined FTIR and LC-MS/MS•Using chemometrics for data analysis•Putative metabolite identification by MS2 spectra</description><subject>agricultural biotechnology</subject><subject>air</subject><subject>biocatalysis</subject><subject>Drying method</subject><subject>flavonoids</subject><subject>Fourier transform infrared spectroscopy</subject><subject>FTIR spectra</subject><subject>Indonesia</subject><subject>LC–MS/MS</subject><subject>liquid chromatography</subject><subject>metabolites</subject><subject>Metabolomics</subject><subject>ovens</subject><subject>principal component analysis</subject><subject>Sida rhombifolia</subject><subject>tandem mass spectrometry</subject><subject>traditional medicine</subject><subject>variance</subject><subject>Xanthine oxidase</subject><issn>1878-8181</issn><issn>1878-8181</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9UE1rGzEQXUoLDYn_QE865rKupF17FcilmDo2uBTs9Cz0MeqO2ZVSSU7sn5B_HbnuIacOAzPMvDcfr6q-MDpllM2_7qfaKD3llDelwEXTfKiumOhELZhgH9_ln6tJSntabE5nXLRX1etmUa-2P3ZEeUuWj-ttrVUCS0bISochjGhS6anhlDD9BR2Vzz16IOGItmAJ-h415hBPBJ7VcFAZgyfBkV3pk9iHUaMLAyrygrknFp2DCD4TG0_of59X9cGmm-qTU0OCyb94Xf1afn9crOrNz4f14tumNuXgXGvXtY3l-k4YoJrpxoiuU2bGuW6p7kBRp7lq3bxjvNVgnXZCNQycpvbO2a65rm4vc59i-HOAlOWIycAwKA_hkGTDZsW7GaUFyi9QE0NKEZx8ijiqeJKMyrP0ci_P0suz9PIifSHdX0hQnnhGiDIZBG_AYgSTpQ34P_obcqqPgQ</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Silviani, Dea</creator><creator>Wahyuni, Wulan Tri</creator><creator>Syafitri, Utami Dyah</creator><creator>Ilmiawati, Auliya</creator><creator>Septaningsih, Dewi Anggraini</creator><creator>Insanu, Muhamad</creator><creator>Aminah, Nanik Siti</creator><creator>Rohman, Abdul</creator><creator>Rafi, Mohamad</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-5225-8703</orcidid></search><sort><creationdate>20230901</creationdate><title>LC-HRMS and FTIR-based metabolomics analysis and xanthine oxidase inhibitory evaluation of Sida rhombifolia with different drying methods</title><author>Silviani, Dea ; Wahyuni, Wulan Tri ; Syafitri, Utami Dyah ; Ilmiawati, Auliya ; Septaningsih, Dewi Anggraini ; Insanu, Muhamad ; Aminah, Nanik Siti ; Rohman, Abdul ; Rafi, Mohamad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c284t-bf743d2b98ce0b1b3c877ac522b40b7ea0fb2a4f67124bedfbf8a31efb0d9fd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>agricultural biotechnology</topic><topic>air</topic><topic>biocatalysis</topic><topic>Drying method</topic><topic>flavonoids</topic><topic>Fourier transform infrared spectroscopy</topic><topic>FTIR spectra</topic><topic>Indonesia</topic><topic>LC–MS/MS</topic><topic>liquid chromatography</topic><topic>metabolites</topic><topic>Metabolomics</topic><topic>ovens</topic><topic>principal component analysis</topic><topic>Sida rhombifolia</topic><topic>tandem mass spectrometry</topic><topic>traditional medicine</topic><topic>variance</topic><topic>Xanthine oxidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silviani, Dea</creatorcontrib><creatorcontrib>Wahyuni, Wulan Tri</creatorcontrib><creatorcontrib>Syafitri, Utami Dyah</creatorcontrib><creatorcontrib>Ilmiawati, Auliya</creatorcontrib><creatorcontrib>Septaningsih, Dewi Anggraini</creatorcontrib><creatorcontrib>Insanu, Muhamad</creatorcontrib><creatorcontrib>Aminah, Nanik Siti</creatorcontrib><creatorcontrib>Rohman, Abdul</creatorcontrib><creatorcontrib>Rafi, Mohamad</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Biocatalysis and agricultural biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silviani, Dea</au><au>Wahyuni, Wulan Tri</au><au>Syafitri, Utami Dyah</au><au>Ilmiawati, Auliya</au><au>Septaningsih, Dewi Anggraini</au><au>Insanu, Muhamad</au><au>Aminah, Nanik Siti</au><au>Rohman, Abdul</au><au>Rafi, Mohamad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LC-HRMS and FTIR-based metabolomics analysis and xanthine oxidase inhibitory evaluation of Sida rhombifolia with different drying methods</atitle><jtitle>Biocatalysis and agricultural biotechnology</jtitle><date>2023-09-01</date><risdate>2023</risdate><volume>52</volume><spage>102833</spage><pages>102833-</pages><artnum>102833</artnum><issn>1878-8181</issn><eissn>1878-8181</eissn><abstract>Sida rhombifolia, known as Sidaguri in Indonesia, has been widely used in traditional medicine because of its bioactive metabolites, particularly flavonoids and steroids. Several factors, including the drying method, can affect the composition and concentrations of metabolites in plants. This study aimed to profile the metabolites and evaluate the xanthine oxidase inhibitory activity of S. rhombifolia dried by different methods. Three drying methods were used: air drying (AD), oven drying (OD), and sun drying (SD). Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and Fourier transform infrared (FTIR)-based untargeted metabolomics were used to profile the metabolites in S. rhombifolia. The FTIR spectra of samples dried by different methods showed similar patterns, with peaks at 3398, 2935, 2856, 1728, 1456, and 1164 cm−1. Metabolite profiling of S. rhombifolia using liquid chromatography–tandem mass spectrometry (LC–MS/MS) identified 24 putative metabolites, with 20, 15, and 16 metabolites found in the AD, OD, and SD extracts, respectively. The principal component analysis demonstrated the separation of S. rhombifolia into groups based on drying method differences. These differences account for 95% (LC–MS/MS) and 99% (FTIR) of the total variance explained. Evaluation of xanthine oxidase inhibition revealed that S. rhombifolia inhibited the enzyme by 22.81% (AD), 35.97% (OD), and 45.93% (SD) at an extract concentration of 50 ppm. The drying method affected the metabolite profile with regard to both the functional groups and amounts of metabolites. Furthermore, the results showed that the xanthine oxidase inhibitory activity depended on the S. rhombifolia drying method and conditions.
[Display omitted]
•Different drying methods can affect the metabolite profile in Sida rhombifolia.•Combined FTIR and LC-MS/MS•Using chemometrics for data analysis•Putative metabolite identification by MS2 spectra</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.bcab.2023.102833</doi><orcidid>https://orcid.org/0000-0002-5225-8703</orcidid></addata></record> |
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| ispartof | Biocatalysis and agricultural biotechnology, 2023-09, Vol.52, p.102833, Article 102833 |
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| source | Alma/SFX Local Collection |
| subjects | agricultural biotechnology air biocatalysis Drying method flavonoids Fourier transform infrared spectroscopy FTIR spectra Indonesia LC–MS/MS liquid chromatography metabolites Metabolomics ovens principal component analysis Sida rhombifolia tandem mass spectrometry traditional medicine variance Xanthine oxidase |
| title | LC-HRMS and FTIR-based metabolomics analysis and xanthine oxidase inhibitory evaluation of Sida rhombifolia with different drying methods |
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