In Vivo Investigation of Supportive Immunotherapeutic Combination of Bifidobacterium infantis 35624 and Doxorubicin in Murine Breast Cancer
The aim of the study is to investigate the anti-tumor effect of Bifidobacterium infantis 35624 in a xenograft model in BALB/c mice injected with 4T1 cells as a support for chemotherapeutic treatments of doxorubicin in vivo. The MTT assay was used to determine the cytotoxicity of doxorubicin against...
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| Veröffentlicht in: | Probiotics and antimicrobial proteins 2023-08, Vol.15 (4), p.880-888 |
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| description | The aim of the study is to investigate the anti-tumor effect of
Bifidobacterium infantis
35624 in a xenograft model in BALB/c mice injected with 4T1 cells as a support for chemotherapeutic treatments of doxorubicin in vivo. The MTT assay was used to determine the cytotoxicity of doxorubicin against cancer cells, and apoptosis was analyzed by using flow cytometry. 4T1 cells (2 × 10
4
cells/mouse) were injected to BALB/c mice, and mice were fed with/without gavage
B. infantis
milk (10
8
CFU/mL) for 14 days and treated with doxorubicin on 5th and 10th days. The weights of the mice were recorded during the study, and the tumor sizes were measured by caliper at the 14th day. CD8 + T cell response was analyzed by using flow cytometer, and the results were compared to control and tumor control groups. The IC
50
value for doxorubicin on 4T1 cell lines was determined as 0.053 ± 0.012 µg/mL. The apoptotic effect of doxorubicin at IC
50
concentration was determined as 82.3% of cells to late apoptosis, 3.6% of cells to pro-apoptosis, and 6.2% of cells to necrosis. The treatment of doxorubicin,
B. infantis
milk, and the combination of them inhibited the tumor volumes by 55.50%, 40.69%, and 75.95%, respectively.
B. infantis
administration significantly enhanced the PHA-induced splenocyte proliferation (
P
|
| doi_str_mv | 10.1007/s12602-021-09899-w |
| format | Article |
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Bifidobacterium infantis
35624 in a xenograft model in BALB/c mice injected with 4T1 cells as a support for chemotherapeutic treatments of doxorubicin in vivo. The MTT assay was used to determine the cytotoxicity of doxorubicin against cancer cells, and apoptosis was analyzed by using flow cytometry. 4T1 cells (2 × 10
4
cells/mouse) were injected to BALB/c mice, and mice were fed with/without gavage
B. infantis
milk (10
8
CFU/mL) for 14 days and treated with doxorubicin on 5th and 10th days. The weights of the mice were recorded during the study, and the tumor sizes were measured by caliper at the 14th day. CD8 + T cell response was analyzed by using flow cytometer, and the results were compared to control and tumor control groups. The IC
50
value for doxorubicin on 4T1 cell lines was determined as 0.053 ± 0.012 µg/mL. The apoptotic effect of doxorubicin at IC
50
concentration was determined as 82.3% of cells to late apoptosis, 3.6% of cells to pro-apoptosis, and 6.2% of cells to necrosis. The treatment of doxorubicin,
B. infantis
milk, and the combination of them inhibited the tumor volumes by 55.50%, 40.69%, and 75.95%, respectively.
B. infantis
administration significantly enhanced the PHA-induced splenocyte proliferation (
P
< 0.05). It was shown that IFN-γ was effective in tumor growth and regression of metastasis. Consequently, the combination of
B. infantis
milk and doxorubicin showed the best anti-tumor effect.</description><identifier>ISSN: 1867-1306</identifier><identifier>EISSN: 1867-1314</identifier><identifier>DOI: 10.1007/s12602-021-09899-w</identifier><identifier>PMID: 35112297</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>antineoplastic activity ; Apoptosis ; Applied Microbiology ; Bifidobacterium infantis ; Bifidobacterium longum subsp. infantis ; Biomedical and Life Sciences ; breast neoplasms ; CD8 antigen ; Chemistry/Food Science ; Cytotoxicity ; Doxorubicin ; drug therapy ; Flow cytometry ; immunotherapy ; Life Sciences ; Lymphocytes T ; Metastases ; metastasis ; mice ; Microbiology ; Milk ; necrosis ; Nutrition ; probiotics ; Protein Science ; splenocytes ; T-lymphocytes ; toxicity testing ; Tumors ; xenotransplantation ; γ-Interferon</subject><ispartof>Probiotics and antimicrobial proteins, 2023-08, Vol.15 (4), p.880-888</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-abf6b991b416db4023a64b44ecd8e44092238e2554864af5c6c3e637ed9ef9e53</citedby><cites>FETCH-LOGICAL-c408t-abf6b991b416db4023a64b44ecd8e44092238e2554864af5c6c3e637ed9ef9e53</cites><orcidid>0000-0002-6993-3883 ; 0000-0001-9457-1311 ; 0000-0002-1180-0682 ; 0000-0002-3852-1974</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12602-021-09899-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12602-021-09899-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35112297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akbaba, Meltem</creatorcontrib><creatorcontrib>Gökmen, Gökhan Gurur</creatorcontrib><creatorcontrib>Kışla, Duygu</creatorcontrib><creatorcontrib>Nalbantsoy, Ayşe</creatorcontrib><title>In Vivo Investigation of Supportive Immunotherapeutic Combination of Bifidobacterium infantis 35624 and Doxorubicin in Murine Breast Cancer</title><title>Probiotics and antimicrobial proteins</title><addtitle>Probiotics & Antimicro. Prot</addtitle><addtitle>Probiotics Antimicrob Proteins</addtitle><description>The aim of the study is to investigate the anti-tumor effect of
Bifidobacterium infantis
35624 in a xenograft model in BALB/c mice injected with 4T1 cells as a support for chemotherapeutic treatments of doxorubicin in vivo. The MTT assay was used to determine the cytotoxicity of doxorubicin against cancer cells, and apoptosis was analyzed by using flow cytometry. 4T1 cells (2 × 10
4
cells/mouse) were injected to BALB/c mice, and mice were fed with/without gavage
B. infantis
milk (10
8
CFU/mL) for 14 days and treated with doxorubicin on 5th and 10th days. The weights of the mice were recorded during the study, and the tumor sizes were measured by caliper at the 14th day. CD8 + T cell response was analyzed by using flow cytometer, and the results were compared to control and tumor control groups. The IC
50
value for doxorubicin on 4T1 cell lines was determined as 0.053 ± 0.012 µg/mL. The apoptotic effect of doxorubicin at IC
50
concentration was determined as 82.3% of cells to late apoptosis, 3.6% of cells to pro-apoptosis, and 6.2% of cells to necrosis. The treatment of doxorubicin,
B. infantis
milk, and the combination of them inhibited the tumor volumes by 55.50%, 40.69%, and 75.95%, respectively.
B. infantis
administration significantly enhanced the PHA-induced splenocyte proliferation (
P
< 0.05). It was shown that IFN-γ was effective in tumor growth and regression of metastasis. Consequently, the combination of
B. infantis
milk and doxorubicin showed the best anti-tumor effect.</description><subject>antineoplastic activity</subject><subject>Apoptosis</subject><subject>Applied Microbiology</subject><subject>Bifidobacterium infantis</subject><subject>Bifidobacterium longum subsp. infantis</subject><subject>Biomedical and Life Sciences</subject><subject>breast neoplasms</subject><subject>CD8 antigen</subject><subject>Chemistry/Food Science</subject><subject>Cytotoxicity</subject><subject>Doxorubicin</subject><subject>drug therapy</subject><subject>Flow cytometry</subject><subject>immunotherapy</subject><subject>Life Sciences</subject><subject>Lymphocytes T</subject><subject>Metastases</subject><subject>metastasis</subject><subject>mice</subject><subject>Microbiology</subject><subject>Milk</subject><subject>necrosis</subject><subject>Nutrition</subject><subject>probiotics</subject><subject>Protein Science</subject><subject>splenocytes</subject><subject>T-lymphocytes</subject><subject>toxicity testing</subject><subject>Tumors</subject><subject>xenotransplantation</subject><subject>γ-Interferon</subject><issn>1867-1306</issn><issn>1867-1314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkctuFDEQRS1ERB7wAyyQJTZsGvzu9pJMSBgpiAWPrWW7q4Ojabux2xP4hvx0OkwYJBYgWSpLdeqWSgeh55S8poS0bwplirCGMNoQ3Wnd3DxCR7RTbUM5FY_3f6IO0XEp14QoxRl5gg65pJQx3R6h23XEX8M24XXcQpnDlZ1DijgN-FOdppTnsAW8Hsca0_wNsp2gzsHjVRpdiHv2NAyhT876GXKoIw5xsHEOBXOpmMA29vgs_Ui5uuBDXNr4Q80hAj7NYMuMVzZ6yE_RwWA3BZ491BP05fzd59X75vLjxXr19rLxgnRzY92gnNbUCap6JwjjVgknBPi-AyGIZox3wKQUnRJ2kF55Doq30GsYNEh-gl7tcqecvtflajOG4mGzsRFSLYZTuTwlCPkvyhSTrKWkEwv68i_0OtUcl0MM64QQXGp1v5vtKJ9TKRkGM-Uw2vzTUGLurZqdVbNYNb-smptl6MVDdHUj9PuR3xoXgO-AsrTiFeQ_u_8ReweRD65I</recordid><startdate>20230801</startdate><enddate>20230801</enddate><creator>Akbaba, Meltem</creator><creator>Gökmen, Gökhan Gurur</creator><creator>Kışla, Duygu</creator><creator>Nalbantsoy, Ayşe</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-6993-3883</orcidid><orcidid>https://orcid.org/0000-0001-9457-1311</orcidid><orcidid>https://orcid.org/0000-0002-1180-0682</orcidid><orcidid>https://orcid.org/0000-0002-3852-1974</orcidid></search><sort><creationdate>20230801</creationdate><title>In Vivo Investigation of Supportive Immunotherapeutic Combination of Bifidobacterium infantis 35624 and Doxorubicin in Murine Breast Cancer</title><author>Akbaba, Meltem ; Gökmen, Gökhan Gurur ; Kışla, Duygu ; Nalbantsoy, Ayşe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-abf6b991b416db4023a64b44ecd8e44092238e2554864af5c6c3e637ed9ef9e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>antineoplastic activity</topic><topic>Apoptosis</topic><topic>Applied Microbiology</topic><topic>Bifidobacterium infantis</topic><topic>Bifidobacterium longum subsp. infantis</topic><topic>Biomedical and Life Sciences</topic><topic>breast neoplasms</topic><topic>CD8 antigen</topic><topic>Chemistry/Food Science</topic><topic>Cytotoxicity</topic><topic>Doxorubicin</topic><topic>drug therapy</topic><topic>Flow cytometry</topic><topic>immunotherapy</topic><topic>Life Sciences</topic><topic>Lymphocytes T</topic><topic>Metastases</topic><topic>metastasis</topic><topic>mice</topic><topic>Microbiology</topic><topic>Milk</topic><topic>necrosis</topic><topic>Nutrition</topic><topic>probiotics</topic><topic>Protein Science</topic><topic>splenocytes</topic><topic>T-lymphocytes</topic><topic>toxicity testing</topic><topic>Tumors</topic><topic>xenotransplantation</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akbaba, Meltem</creatorcontrib><creatorcontrib>Gökmen, Gökhan Gurur</creatorcontrib><creatorcontrib>Kışla, Duygu</creatorcontrib><creatorcontrib>Nalbantsoy, Ayşe</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Probiotics and antimicrobial proteins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akbaba, Meltem</au><au>Gökmen, Gökhan Gurur</au><au>Kışla, Duygu</au><au>Nalbantsoy, Ayşe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vivo Investigation of Supportive Immunotherapeutic Combination of Bifidobacterium infantis 35624 and Doxorubicin in Murine Breast Cancer</atitle><jtitle>Probiotics and antimicrobial proteins</jtitle><stitle>Probiotics & Antimicro. Prot</stitle><addtitle>Probiotics Antimicrob Proteins</addtitle><date>2023-08-01</date><risdate>2023</risdate><volume>15</volume><issue>4</issue><spage>880</spage><epage>888</epage><pages>880-888</pages><issn>1867-1306</issn><eissn>1867-1314</eissn><abstract>The aim of the study is to investigate the anti-tumor effect of
Bifidobacterium infantis
35624 in a xenograft model in BALB/c mice injected with 4T1 cells as a support for chemotherapeutic treatments of doxorubicin in vivo. The MTT assay was used to determine the cytotoxicity of doxorubicin against cancer cells, and apoptosis was analyzed by using flow cytometry. 4T1 cells (2 × 10
4
cells/mouse) were injected to BALB/c mice, and mice were fed with/without gavage
B. infantis
milk (10
8
CFU/mL) for 14 days and treated with doxorubicin on 5th and 10th days. The weights of the mice were recorded during the study, and the tumor sizes were measured by caliper at the 14th day. CD8 + T cell response was analyzed by using flow cytometer, and the results were compared to control and tumor control groups. The IC
50
value for doxorubicin on 4T1 cell lines was determined as 0.053 ± 0.012 µg/mL. The apoptotic effect of doxorubicin at IC
50
concentration was determined as 82.3% of cells to late apoptosis, 3.6% of cells to pro-apoptosis, and 6.2% of cells to necrosis. The treatment of doxorubicin,
B. infantis
milk, and the combination of them inhibited the tumor volumes by 55.50%, 40.69%, and 75.95%, respectively.
B. infantis
administration significantly enhanced the PHA-induced splenocyte proliferation (
P
< 0.05). It was shown that IFN-γ was effective in tumor growth and regression of metastasis. Consequently, the combination of
B. infantis
milk and doxorubicin showed the best anti-tumor effect.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35112297</pmid><doi>10.1007/s12602-021-09899-w</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-6993-3883</orcidid><orcidid>https://orcid.org/0000-0001-9457-1311</orcidid><orcidid>https://orcid.org/0000-0002-1180-0682</orcidid><orcidid>https://orcid.org/0000-0002-3852-1974</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1867-1306 |
| ispartof | Probiotics and antimicrobial proteins, 2023-08, Vol.15 (4), p.880-888 |
| issn | 1867-1306 1867-1314 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153156400 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | antineoplastic activity Apoptosis Applied Microbiology Bifidobacterium infantis Bifidobacterium longum subsp. infantis Biomedical and Life Sciences breast neoplasms CD8 antigen Chemistry/Food Science Cytotoxicity Doxorubicin drug therapy Flow cytometry immunotherapy Life Sciences Lymphocytes T Metastases metastasis mice Microbiology Milk necrosis Nutrition probiotics Protein Science splenocytes T-lymphocytes toxicity testing Tumors xenotransplantation γ-Interferon |
| title | In Vivo Investigation of Supportive Immunotherapeutic Combination of Bifidobacterium infantis 35624 and Doxorubicin in Murine Breast Cancer |
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