Development and evaluation of sandwich ELISA for detection and quantification of staphylococcal enterotoxin‐A in food
Staphylococcus aureus is an opportunistic zoonotic pathogen which secretes 24 different types of enterotoxins (SEs) and enterotoxin‐like (SEls) proteins. Classical enterotoxins (SEA–SEE) are responsible for >95% of food poisoning outbreaks, of which SEA alone is responsible for >75% of them. T...
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| Veröffentlicht in: | Journal of food safety 2024-04, Vol.44 (2), p.n/a |
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| creator | Singh, Mamta Agrawal, Ravi Kant Singh, Bhoj Raj Mendiratta, Sanjod Kumar Kumar, Deepak Kumar, Bablu |
| description | Staphylococcus aureus is an opportunistic zoonotic pathogen which secretes 24 different types of enterotoxins (SEs) and enterotoxin‐like (SEls) proteins. Classical enterotoxins (SEA–SEE) are responsible for >95% of food poisoning outbreaks, of which SEA alone is responsible for >75% of them. This study was undertaken to develop a sandwich enzyme‐linked immunosorbent assay (ELISA) for sensitive, specific, and quantitative detection of staphylococcal enterotoxins‐A in food. Optimization of sandwich ELISA was attempted in two ways: rabbit polyclonal anti‐SEA as a capture antibody and mouse monoclonal anti‐SEA as a detector antibody, and vice versa. In the optimization of sandwich ELISA, mouse monoclonal anti‐SEA as a capture antibody and rabbit polyclonal anti‐SEA as a detector antibody yielded the highest sensitivity of 0.5–0.75 ng mL−1. The developed assay was found to be highly specific and exhibited equivalent sensitivity to a commercial kit. The developed sandwich ELISA may be utilized for the detection of staphylococcal enterotoxin‐A in food as a cheap alternative to available commercial kits. The developed sandwich ELISA may be useful for microbiological quality assurance of foods, especially in resource‐limited developing countries.
Test for ensuring Microbiological Safety of Food |
| doi_str_mv | 10.1111/jfs.13114 |
| format | Article |
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Test for ensuring Microbiological Safety of Food</description><identifier>ISSN: 0149-6085</identifier><identifier>EISSN: 1745-4565</identifier><identifier>DOI: 10.1111/jfs.13114</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Antibodies ; detection ; Developing countries ; ELISA ; enterotoxins ; Enzyme-linked immunosorbent assay ; Food ; Food contamination ; Food poisoning ; Food quality ; food safety ; foodborne intoxication ; LDCs ; mice ; microbiological quality ; Opportunist infection ; Optimization ; pathogens ; Quality assurance ; quality control ; Rabbits ; SEA ; Sensitivity ; staphylococcal enterotoxin A ; Staphylococcus aureus</subject><ispartof>Journal of food safety, 2024-04, Vol.44 (2), p.n/a</ispartof><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2904-4fa075d2cac5f7c77cd91865e2d08dcd717fb341b1420e432712bf9da73be9683</cites><orcidid>0000-0002-1653-9495 ; 0000-0002-1352-4570</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfs.13114$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfs.13114$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Singh, Mamta</creatorcontrib><creatorcontrib>Agrawal, Ravi Kant</creatorcontrib><creatorcontrib>Singh, Bhoj Raj</creatorcontrib><creatorcontrib>Mendiratta, Sanjod Kumar</creatorcontrib><creatorcontrib>Kumar, Deepak</creatorcontrib><creatorcontrib>Kumar, Bablu</creatorcontrib><title>Development and evaluation of sandwich ELISA for detection and quantification of staphylococcal enterotoxin‐A in food</title><title>Journal of food safety</title><description>Staphylococcus aureus is an opportunistic zoonotic pathogen which secretes 24 different types of enterotoxins (SEs) and enterotoxin‐like (SEls) proteins. Classical enterotoxins (SEA–SEE) are responsible for >95% of food poisoning outbreaks, of which SEA alone is responsible for >75% of them. This study was undertaken to develop a sandwich enzyme‐linked immunosorbent assay (ELISA) for sensitive, specific, and quantitative detection of staphylococcal enterotoxins‐A in food. Optimization of sandwich ELISA was attempted in two ways: rabbit polyclonal anti‐SEA as a capture antibody and mouse monoclonal anti‐SEA as a detector antibody, and vice versa. In the optimization of sandwich ELISA, mouse monoclonal anti‐SEA as a capture antibody and rabbit polyclonal anti‐SEA as a detector antibody yielded the highest sensitivity of 0.5–0.75 ng mL−1. The developed assay was found to be highly specific and exhibited equivalent sensitivity to a commercial kit. The developed sandwich ELISA may be utilized for the detection of staphylococcal enterotoxin‐A in food as a cheap alternative to available commercial kits. The developed sandwich ELISA may be useful for microbiological quality assurance of foods, especially in resource‐limited developing countries.
Test for ensuring Microbiological Safety of Food</description><subject>Antibodies</subject><subject>detection</subject><subject>Developing countries</subject><subject>ELISA</subject><subject>enterotoxins</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Food</subject><subject>Food contamination</subject><subject>Food poisoning</subject><subject>Food quality</subject><subject>food safety</subject><subject>foodborne intoxication</subject><subject>LDCs</subject><subject>mice</subject><subject>microbiological quality</subject><subject>Opportunist infection</subject><subject>Optimization</subject><subject>pathogens</subject><subject>Quality assurance</subject><subject>quality control</subject><subject>Rabbits</subject><subject>SEA</subject><subject>Sensitivity</subject><subject>staphylococcal enterotoxin A</subject><subject>Staphylococcus aureus</subject><issn>0149-6085</issn><issn>1745-4565</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kctKAzEUhoMoWKsL3yDgRhdjc53MLEutNwouquuQ5kJTppN2MmPtzkfwGX0SYysIgocfDpzznZ8DPwDnGF3jVIOFi9eYYswOQA8LxjPGc34IegizMstRwY_BSYwLhGhOCO2BzY19tVVYLW3dQlUbaF9V1anWhxoGB2Mabbyew_HkYTqELjTQ2Nbq3f4bX3eqbr3z-vekVav5tgo6aK0qmHxtE9rw5uvP948h9HVyCeYUHDlVRXv20_vg5Xb8PLrPJk93D6PhJNOkRCxjTiHBDdFKcye0ENqUuMi5JQYVRhuBhZtRhmeYEWQZJQKTmSuNEnRmy7ygfXC59101Yd3Z2Mqlj9pWlapt6KKkmCcRmtQHF3_QReiaOn0nKWKs5KKgIlFXe0o3IcbGOrlq_FI1W4mR_I5ApgjkLoLEDvbsxld2-z8oH2-n-4svpzeJzQ</recordid><startdate>202404</startdate><enddate>202404</enddate><creator>Singh, Mamta</creator><creator>Agrawal, Ravi Kant</creator><creator>Singh, Bhoj Raj</creator><creator>Mendiratta, Sanjod Kumar</creator><creator>Kumar, Deepak</creator><creator>Kumar, Bablu</creator><general>John Wiley & Sons, Inc</general><general>Blackwell Publishers Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7T2</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>P64</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-1653-9495</orcidid><orcidid>https://orcid.org/0000-0002-1352-4570</orcidid></search><sort><creationdate>202404</creationdate><title>Development and evaluation of sandwich ELISA for detection and quantification of staphylococcal enterotoxin‐A in food</title><author>Singh, Mamta ; Agrawal, Ravi Kant ; Singh, Bhoj Raj ; Mendiratta, Sanjod Kumar ; Kumar, Deepak ; Kumar, Bablu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2904-4fa075d2cac5f7c77cd91865e2d08dcd717fb341b1420e432712bf9da73be9683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antibodies</topic><topic>detection</topic><topic>Developing countries</topic><topic>ELISA</topic><topic>enterotoxins</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Food</topic><topic>Food contamination</topic><topic>Food poisoning</topic><topic>Food quality</topic><topic>food safety</topic><topic>foodborne intoxication</topic><topic>LDCs</topic><topic>mice</topic><topic>microbiological quality</topic><topic>Opportunist infection</topic><topic>Optimization</topic><topic>pathogens</topic><topic>Quality assurance</topic><topic>quality control</topic><topic>Rabbits</topic><topic>SEA</topic><topic>Sensitivity</topic><topic>staphylococcal enterotoxin A</topic><topic>Staphylococcus aureus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Mamta</creatorcontrib><creatorcontrib>Agrawal, Ravi Kant</creatorcontrib><creatorcontrib>Singh, Bhoj Raj</creatorcontrib><creatorcontrib>Mendiratta, Sanjod Kumar</creatorcontrib><creatorcontrib>Kumar, Deepak</creatorcontrib><creatorcontrib>Kumar, Bablu</creatorcontrib><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of food safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Mamta</au><au>Agrawal, Ravi Kant</au><au>Singh, Bhoj Raj</au><au>Mendiratta, Sanjod Kumar</au><au>Kumar, Deepak</au><au>Kumar, Bablu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and evaluation of sandwich ELISA for detection and quantification of staphylococcal enterotoxin‐A in food</atitle><jtitle>Journal of food safety</jtitle><date>2024-04</date><risdate>2024</risdate><volume>44</volume><issue>2</issue><epage>n/a</epage><issn>0149-6085</issn><eissn>1745-4565</eissn><abstract>Staphylococcus aureus is an opportunistic zoonotic pathogen which secretes 24 different types of enterotoxins (SEs) and enterotoxin‐like (SEls) proteins. Classical enterotoxins (SEA–SEE) are responsible for >95% of food poisoning outbreaks, of which SEA alone is responsible for >75% of them. This study was undertaken to develop a sandwich enzyme‐linked immunosorbent assay (ELISA) for sensitive, specific, and quantitative detection of staphylococcal enterotoxins‐A in food. Optimization of sandwich ELISA was attempted in two ways: rabbit polyclonal anti‐SEA as a capture antibody and mouse monoclonal anti‐SEA as a detector antibody, and vice versa. In the optimization of sandwich ELISA, mouse monoclonal anti‐SEA as a capture antibody and rabbit polyclonal anti‐SEA as a detector antibody yielded the highest sensitivity of 0.5–0.75 ng mL−1. The developed assay was found to be highly specific and exhibited equivalent sensitivity to a commercial kit. The developed sandwich ELISA may be utilized for the detection of staphylococcal enterotoxin‐A in food as a cheap alternative to available commercial kits. The developed sandwich ELISA may be useful for microbiological quality assurance of foods, especially in resource‐limited developing countries.
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| subjects | Antibodies detection Developing countries ELISA enterotoxins Enzyme-linked immunosorbent assay Food Food contamination Food poisoning Food quality food safety foodborne intoxication LDCs mice microbiological quality Opportunist infection Optimization pathogens Quality assurance quality control Rabbits SEA Sensitivity staphylococcal enterotoxin A Staphylococcus aureus |
| title | Development and evaluation of sandwich ELISA for detection and quantification of staphylococcal enterotoxin‐A in food |
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