Directed Evolution of an Alginate Lyase from Flammeovirga sp. for Seaweed Fertilizer Production from the Brown Seaweed Laminaria japonica

An alginate lyase (FsAly7) from Flammeovirga sp. was engineered by directed evolution to improve its optimum temperature and thermostability. The optimum temperature of the positive mutant mFsAly7 (FsAly7-Ser43Pro) was increased by 5 °C, and the thermal inactivation half-lives at 40 and 45 °C were 4...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2025-01, Vol.73 (2), p.1468-1477
Hauptverfasser:	Jiang, Jun, Hu, Zhiyi, Wang, Yue, Jiang, Zhengqiang, Yan, Qiaojuan, Yang, Shaoqing
Format:	Artikel
Sprache:	eng
Schlagworte:	Biotechnology and Biological Transformations Directed Molecular Evolution Edible Seaweeds Enzyme Stability Fertilizers - analysis Laminaria - chemistry Laminaria - enzymology Laminaria - genetics Laminaria - growth & development Laminaria - metabolism Polysaccharide-Lyases - chemistry Polysaccharide-Lyases - genetics Polysaccharide-Lyases - metabolism Seaweed - chemistry Seaweed - genetics Seaweed - metabolism
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