Dentin remineralization induced by experimental composites containing calcium orthophosphate particles
This study aimed to verify if composites containing dicalcium phosphate dihydrate particles (DCPD) are able to induce dentin remineralization in vitro. Additionally, the mechanical properties of the materials were tested. Four composites with 50 vol% inorganic content and 1 BisGMA: 1 TEGDMA (mols) w...
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| creator | Campos, Amanda Lopes Chiari, Marina Damasceno e Souza de Carvalho Vela, Beatriz Fonseca Trinca, Rafael Bergamo de Souza Balbinot, Gabriela Collares, Fabrício Mezzomo Braga, Roberto Ruggiero |
| description | This study aimed to verify if composites containing dicalcium phosphate dihydrate particles (DCPD) are able to induce dentin remineralization in vitro. Additionally, the mechanical properties of the materials were tested.
Four composites with 50 vol% inorganic content and 1 BisGMA: 1 TEGDMA (mols) were prepared, with different DCPD:glass ratios (50:0, 40:10, 30:20 and 0:50). Ca2 + release in water was monitored for 8 weeks using inductively coupled plasma optical emission spectrometry (n = 3). Composites were applied to artificial lesions (180 μm in depth) prepared in dentin discs and the specimens were kept in simulated body fluid for 8 weeks (n = 8–10). Dentin elastic modulus (EM) and hardness (H) across the lesion were determined by nanoindentation (5 mN, 5 s). Mineral density was determined by microCT. Composite degree of conversion (DC) was determined by near-FTIR spectroscopy (n = 3). Fracture strength and elastic modulus were determined using biaxial flexural test (n = 10). Data were analysed by ANOVA/Tukey test, except for mineral density (Kruskal-Wallis, alpha:0.05).
Ca2+ release increase linearly with DCPD fraction in the composite (p |
| doi_str_mv | 10.1016/j.dental.2024.12.004 |
| format | Article |
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Four composites with 50 vol% inorganic content and 1 BisGMA: 1 TEGDMA (mols) were prepared, with different DCPD:glass ratios (50:0, 40:10, 30:20 and 0:50). Ca2 + release in water was monitored for 8 weeks using inductively coupled plasma optical emission spectrometry (n = 3). Composites were applied to artificial lesions (180 μm in depth) prepared in dentin discs and the specimens were kept in simulated body fluid for 8 weeks (n = 8–10). Dentin elastic modulus (EM) and hardness (H) across the lesion were determined by nanoindentation (5 mN, 5 s). Mineral density was determined by microCT. Composite degree of conversion (DC) was determined by near-FTIR spectroscopy (n = 3). Fracture strength and elastic modulus were determined using biaxial flexural test (n = 10). Data were analysed by ANOVA/Tukey test, except for mineral density (Kruskal-Wallis, alpha:0.05).
Ca2+ release increase linearly with DCPD fraction in the composite (p < 0.001). Lesions kept in contact with composites containing 40 % and 50 % DCPD presented significant increases in EM and H in the outer region (0–90 μm) and in EM in the inner region (90–180 μm) compared to the negative control. MicroCT was not able to differentiate among treatments. DCPD-containing composites presented DC higher than the control (p < 0.01). Flexural strength and modulus were inversely related to DCPD content (p < 0.001).
The composite containing 40 vol% DCPD presented the best compromise between mechanical properties and remineralization potential.
•A linear relationship between composite DCPD fraction and Ca2+ release was verified.•Composites containing 40 % and 50 % DCPD presented similar remineralization results.•The composite with 40 % DCPD presented higher flexural strength than 50 % DCPD.•Nanoindentation was more discriminative than microCT to quantify remineralization.</description><identifier>ISSN: 0109-5641</identifier><identifier>ISSN: 1879-0097</identifier><identifier>EISSN: 1879-0097</identifier><identifier>DOI: 10.1016/j.dental.2024.12.004</identifier><identifier>PMID: 39732611</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Composite ; Nanoindentation ; Remineralization</subject><ispartof>Dental materials, 2024-12</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1561-68bb7f5330e84e4c76b0cd6f51b37a9a22487f661358f7675b1540e0688e61ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S010956412400349X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39732611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Campos, Amanda Lopes</creatorcontrib><creatorcontrib>Chiari, Marina Damasceno e Souza de Carvalho</creatorcontrib><creatorcontrib>Vela, Beatriz Fonseca</creatorcontrib><creatorcontrib>Trinca, Rafael Bergamo</creatorcontrib><creatorcontrib>de Souza Balbinot, Gabriela</creatorcontrib><creatorcontrib>Collares, Fabrício Mezzomo</creatorcontrib><creatorcontrib>Braga, Roberto Ruggiero</creatorcontrib><title>Dentin remineralization induced by experimental composites containing calcium orthophosphate particles</title><title>Dental materials</title><addtitle>Dent Mater</addtitle><description>This study aimed to verify if composites containing dicalcium phosphate dihydrate particles (DCPD) are able to induce dentin remineralization in vitro. Additionally, the mechanical properties of the materials were tested.
Four composites with 50 vol% inorganic content and 1 BisGMA: 1 TEGDMA (mols) were prepared, with different DCPD:glass ratios (50:0, 40:10, 30:20 and 0:50). Ca2 + release in water was monitored for 8 weeks using inductively coupled plasma optical emission spectrometry (n = 3). Composites were applied to artificial lesions (180 μm in depth) prepared in dentin discs and the specimens were kept in simulated body fluid for 8 weeks (n = 8–10). Dentin elastic modulus (EM) and hardness (H) across the lesion were determined by nanoindentation (5 mN, 5 s). Mineral density was determined by microCT. Composite degree of conversion (DC) was determined by near-FTIR spectroscopy (n = 3). Fracture strength and elastic modulus were determined using biaxial flexural test (n = 10). Data were analysed by ANOVA/Tukey test, except for mineral density (Kruskal-Wallis, alpha:0.05).
Ca2+ release increase linearly with DCPD fraction in the composite (p < 0.001). Lesions kept in contact with composites containing 40 % and 50 % DCPD presented significant increases in EM and H in the outer region (0–90 μm) and in EM in the inner region (90–180 μm) compared to the negative control. MicroCT was not able to differentiate among treatments. DCPD-containing composites presented DC higher than the control (p < 0.01). Flexural strength and modulus were inversely related to DCPD content (p < 0.001).
The composite containing 40 vol% DCPD presented the best compromise between mechanical properties and remineralization potential.
•A linear relationship between composite DCPD fraction and Ca2+ release was verified.•Composites containing 40 % and 50 % DCPD presented similar remineralization results.•The composite with 40 % DCPD presented higher flexural strength than 50 % DCPD.•Nanoindentation was more discriminative than microCT to quantify remineralization.</description><subject>Composite</subject><subject>Nanoindentation</subject><subject>Remineralization</subject><issn>0109-5641</issn><issn>1879-0097</issn><issn>1879-0097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE1P3DAQhi3UCpaPf4BQjlySehzHdi5ICNpSaaVe2rPlOBPWq8QOdlKV_npMl3LsaUajZ-bVPIRcAq2Agvi0r3r0ixkrRhmvgFWU8iOyASXbktJWfiAbCrQtG8HhhJymtKeZYC0ck5O6lTUTABsy3OcjzhcRJ-cxmtH9MYsLvnC-Xy32Rfdc4O8Zo5v-phU2THNIbsGU2zxx3vnHwprRunUqQlx2Yd6FNO_MgsVs4uLsiOmcfBzMmPDirZ6Rn18-_7h7KLffv367u92WFhoBpVBdJ4emrikqjtxK0VHbi6GBrpamNYxxJQchoG7UIIVsOmg4RSqUQgHG1mfk-nB3juFpxbToySWL42g8hjXpGnirFOOcZpQfUBtDShEHPecnTXzWQPWrYb3XB8P61bAGprO_vHb1lrB2E_bvS_-UZuDmAGD-85fDqJN16LNLF9Euug_u_wkv1wKQSQ</recordid><startdate>20241227</startdate><enddate>20241227</enddate><creator>Campos, Amanda Lopes</creator><creator>Chiari, Marina Damasceno e Souza de Carvalho</creator><creator>Vela, Beatriz Fonseca</creator><creator>Trinca, Rafael Bergamo</creator><creator>de Souza Balbinot, Gabriela</creator><creator>Collares, Fabrício Mezzomo</creator><creator>Braga, Roberto Ruggiero</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20241227</creationdate><title>Dentin remineralization induced by experimental composites containing calcium orthophosphate particles</title><author>Campos, Amanda Lopes ; Chiari, Marina Damasceno e Souza de Carvalho ; Vela, Beatriz Fonseca ; Trinca, Rafael Bergamo ; de Souza Balbinot, Gabriela ; Collares, Fabrício Mezzomo ; Braga, Roberto Ruggiero</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1561-68bb7f5330e84e4c76b0cd6f51b37a9a22487f661358f7675b1540e0688e61ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Composite</topic><topic>Nanoindentation</topic><topic>Remineralization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Campos, Amanda Lopes</creatorcontrib><creatorcontrib>Chiari, Marina Damasceno e Souza de Carvalho</creatorcontrib><creatorcontrib>Vela, Beatriz Fonseca</creatorcontrib><creatorcontrib>Trinca, Rafael Bergamo</creatorcontrib><creatorcontrib>de Souza Balbinot, Gabriela</creatorcontrib><creatorcontrib>Collares, Fabrício Mezzomo</creatorcontrib><creatorcontrib>Braga, Roberto Ruggiero</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Dental materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Campos, Amanda Lopes</au><au>Chiari, Marina Damasceno e Souza de Carvalho</au><au>Vela, Beatriz Fonseca</au><au>Trinca, Rafael Bergamo</au><au>de Souza Balbinot, Gabriela</au><au>Collares, Fabrício Mezzomo</au><au>Braga, Roberto Ruggiero</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dentin remineralization induced by experimental composites containing calcium orthophosphate particles</atitle><jtitle>Dental materials</jtitle><addtitle>Dent Mater</addtitle><date>2024-12-27</date><risdate>2024</risdate><issn>0109-5641</issn><issn>1879-0097</issn><eissn>1879-0097</eissn><abstract>This study aimed to verify if composites containing dicalcium phosphate dihydrate particles (DCPD) are able to induce dentin remineralization in vitro. Additionally, the mechanical properties of the materials were tested.
Four composites with 50 vol% inorganic content and 1 BisGMA: 1 TEGDMA (mols) were prepared, with different DCPD:glass ratios (50:0, 40:10, 30:20 and 0:50). Ca2 + release in water was monitored for 8 weeks using inductively coupled plasma optical emission spectrometry (n = 3). Composites were applied to artificial lesions (180 μm in depth) prepared in dentin discs and the specimens were kept in simulated body fluid for 8 weeks (n = 8–10). Dentin elastic modulus (EM) and hardness (H) across the lesion were determined by nanoindentation (5 mN, 5 s). Mineral density was determined by microCT. Composite degree of conversion (DC) was determined by near-FTIR spectroscopy (n = 3). Fracture strength and elastic modulus were determined using biaxial flexural test (n = 10). Data were analysed by ANOVA/Tukey test, except for mineral density (Kruskal-Wallis, alpha:0.05).
Ca2+ release increase linearly with DCPD fraction in the composite (p < 0.001). Lesions kept in contact with composites containing 40 % and 50 % DCPD presented significant increases in EM and H in the outer region (0–90 μm) and in EM in the inner region (90–180 μm) compared to the negative control. MicroCT was not able to differentiate among treatments. DCPD-containing composites presented DC higher than the control (p < 0.01). Flexural strength and modulus were inversely related to DCPD content (p < 0.001).
The composite containing 40 vol% DCPD presented the best compromise between mechanical properties and remineralization potential.
•A linear relationship between composite DCPD fraction and Ca2+ release was verified.•Composites containing 40 % and 50 % DCPD presented similar remineralization results.•The composite with 40 % DCPD presented higher flexural strength than 50 % DCPD.•Nanoindentation was more discriminative than microCT to quantify remineralization.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>39732611</pmid><doi>10.1016/j.dental.2024.12.004</doi></addata></record> |
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| subjects | Composite Nanoindentation Remineralization |
| title | Dentin remineralization induced by experimental composites containing calcium orthophosphate particles |
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