Carbon-based light addressable potential aptasensor based on the synergy of C-MXene@rGO and OPD@NGQDs for low-density lipoprotein detection
A novel carbon-based light-addressable potentiometric aptasensor (C-LAPS) was constructed for detection low-density lipoprotein (LDL) in serum. Carboxylated Ti 3 C 2 MXene @reduced graphene oxide (C-MXene@rGO) was used as interface and o-phenylenediamine functionalized nitrogen-doped graphene quantu...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2025-01, Vol.192 (1), p.35, Article 35 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Li, Guiyin Wu, Guangxiong Huang, Qing Dong, Shuaikang Zhou, Yu Lu, Mei Liang, Jintao Zhou, Xueqing Zhou, Zhide |
| description | A novel carbon-based light-addressable potentiometric aptasensor (C-LAPS) was constructed for detection low-density lipoprotein (LDL) in serum. Carboxylated Ti
3
C
2
MXene @reduced graphene oxide (C-MXene@rGO) was used as interface and o-phenylenediamine functionalized nitrogen-doped graphene quantum dots (OPD@NGQDs) as the photoelectric conversion element. The photosensitive layers composed of OPD@NGQDs/C-MXene@rGO exhibit superior photoelectric conversion efficiency and excellent biocompatibility, which contribute to an improved response signal. When LDL reacts with the LDL aptamer (LDL
Apt
) immobilized on the photosensitive layers to form LDL-LDL
Apt
complexes, the reaction process can induce the modification of the surface potential in the photosensitive layer, leading to potential shift observed through the I-V curves. The experimental conditions were successfully optimized with few planned tests by applying the Box-Behnken design and response surface methodology aspects of the Design-Expert software. Under the optimal condition, the potential shift had a linear relationship with concentrations of LDL from 0.02 to 0.30 μg/mL. The limit of detection (LOD) was 5.88 ng/mL (S/N = 3) and the sensitivity was 315.20 mV/μg·mL
−1
. In addition, the LDL C-LAPS demonstrated excellent specificity, reproducibility, and stability in detecting LDL. The sensor performed well in quantifying LDL in real samples. Therefore, the LDL C-LAPS has the potential for clinical applications.
Graphical Abstract |
| doi_str_mv | 10.1007/s00604-024-06909-w |
| format | Article |
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3
C
2
MXene @reduced graphene oxide (C-MXene@rGO) was used as interface and o-phenylenediamine functionalized nitrogen-doped graphene quantum dots (OPD@NGQDs) as the photoelectric conversion element. The photosensitive layers composed of OPD@NGQDs/C-MXene@rGO exhibit superior photoelectric conversion efficiency and excellent biocompatibility, which contribute to an improved response signal. When LDL reacts with the LDL aptamer (LDL
Apt
) immobilized on the photosensitive layers to form LDL-LDL
Apt
complexes, the reaction process can induce the modification of the surface potential in the photosensitive layer, leading to potential shift observed through the I-V curves. The experimental conditions were successfully optimized with few planned tests by applying the Box-Behnken design and response surface methodology aspects of the Design-Expert software. Under the optimal condition, the potential shift had a linear relationship with concentrations of LDL from 0.02 to 0.30 μg/mL. The limit of detection (LOD) was 5.88 ng/mL (S/N = 3) and the sensitivity was 315.20 mV/μg·mL
−1
. In addition, the LDL C-LAPS demonstrated excellent specificity, reproducibility, and stability in detecting LDL. The sensor performed well in quantifying LDL in real samples. Therefore, the LDL C-LAPS has the potential for clinical applications.
Graphical Abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>ISSN: 1436-5073</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-024-06909-w</identifier><identifier>PMID: 39729216</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Aptamers, Nucleotide - chemistry ; Biocompatibility ; Biosensing Techniques - methods ; Carbon ; Carbon - chemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Density ; Electric potential ; Electrochemical Techniques - instrumentation ; Electrochemical Techniques - methods ; Graphene ; Graphite - chemistry ; Humans ; Light ; Limit of Detection ; Lipoproteins, LDL - blood ; Lipoproteins, LDL - chemistry ; Microengineering ; MXenes ; Nanochemistry ; Nanotechnology ; Phenylenediamine ; Phenylenediamines - chemistry ; Photoelectricity ; Photosensitivity ; Quantum dots ; Quantum Dots - chemistry ; Response surface methodology ; Titanium - chemistry</subject><ispartof>Mikrochimica acta (1966), 2025-01, Vol.192 (1), p.35, Article 35</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024 Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><rights>Copyright Springer Nature B.V. 2025</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-11f910bb10fa342beac9ae64a30343e1934d0a64a0d95208332610a57e4825d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-024-06909-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-024-06909-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39729216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Guiyin</creatorcontrib><creatorcontrib>Wu, Guangxiong</creatorcontrib><creatorcontrib>Huang, Qing</creatorcontrib><creatorcontrib>Dong, Shuaikang</creatorcontrib><creatorcontrib>Zhou, Yu</creatorcontrib><creatorcontrib>Lu, Mei</creatorcontrib><creatorcontrib>Liang, Jintao</creatorcontrib><creatorcontrib>Zhou, Xueqing</creatorcontrib><creatorcontrib>Zhou, Zhide</creatorcontrib><title>Carbon-based light addressable potential aptasensor based on the synergy of C-MXene@rGO and OPD@NGQDs for low-density lipoprotein detection</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>A novel carbon-based light-addressable potentiometric aptasensor (C-LAPS) was constructed for detection low-density lipoprotein (LDL) in serum. Carboxylated Ti
3
C
2
MXene @reduced graphene oxide (C-MXene@rGO) was used as interface and o-phenylenediamine functionalized nitrogen-doped graphene quantum dots (OPD@NGQDs) as the photoelectric conversion element. The photosensitive layers composed of OPD@NGQDs/C-MXene@rGO exhibit superior photoelectric conversion efficiency and excellent biocompatibility, which contribute to an improved response signal. When LDL reacts with the LDL aptamer (LDL
Apt
) immobilized on the photosensitive layers to form LDL-LDL
Apt
complexes, the reaction process can induce the modification of the surface potential in the photosensitive layer, leading to potential shift observed through the I-V curves. The experimental conditions were successfully optimized with few planned tests by applying the Box-Behnken design and response surface methodology aspects of the Design-Expert software. Under the optimal condition, the potential shift had a linear relationship with concentrations of LDL from 0.02 to 0.30 μg/mL. The limit of detection (LOD) was 5.88 ng/mL (S/N = 3) and the sensitivity was 315.20 mV/μg·mL
−1
. In addition, the LDL C-LAPS demonstrated excellent specificity, reproducibility, and stability in detecting LDL. The sensor performed well in quantifying LDL in real samples. Therefore, the LDL C-LAPS has the potential for clinical applications.
Graphical Abstract</description><subject>Analytical Chemistry</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Biocompatibility</subject><subject>Biosensing Techniques - methods</subject><subject>Carbon</subject><subject>Carbon - chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Density</subject><subject>Electric potential</subject><subject>Electrochemical Techniques - instrumentation</subject><subject>Electrochemical Techniques - methods</subject><subject>Graphene</subject><subject>Graphite - chemistry</subject><subject>Humans</subject><subject>Light</subject><subject>Limit of Detection</subject><subject>Lipoproteins, LDL - blood</subject><subject>Lipoproteins, LDL - chemistry</subject><subject>Microengineering</subject><subject>MXenes</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Phenylenediamine</subject><subject>Phenylenediamines - chemistry</subject><subject>Photoelectricity</subject><subject>Photosensitivity</subject><subject>Quantum dots</subject><subject>Quantum Dots - chemistry</subject><subject>Response surface methodology</subject><subject>Titanium - chemistry</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV1rFDEUhoNY7Fr9A15IwBtvYk8-JrO5q2x1FaqroODdkJmc2U6ZTcYky7K_wT9t7LQVvPAihJDnfc6Bl5AXHN5wgPo8AWhQDEQ52oBhh0dkwZXUrIJaPiYLAKGZ1LU4JU9TugHgtRbqCTmVphZGcL0gv1Y2tsGz1iZ0dBy215la5yKmZNsR6RQy-jzYkdopF8anEOkMB0_zNdJ09Bi3Rxp6umKffqDHi7jeUOsd3Xy5vPi8_nqZaF9SYzgwVwRDPpZBU5hicQ-eOszY5SH4Z-Skt2PC53f3Gfn-_t231Qd2tVl_XL29Yp2odGac94ZD23LorVSiRdsZi1pZCVJJ5EYqB7a8wZlKwFJKoTnYqka1FJVT8oy8nr1lg597TLnZDanDcbQewz41kitTKS3rZUFf_YPehH30Zbtbqq64NKJQYqa6GFKK2DdTHHY2HhsOzZ-qmrmqplTV3FbVHEro5Z163-7QPUTuuymAnIFUvvwW49_Z_9H-BipJnzg</recordid><startdate>20250101</startdate><enddate>20250101</enddate><creator>Li, Guiyin</creator><creator>Wu, Guangxiong</creator><creator>Huang, Qing</creator><creator>Dong, Shuaikang</creator><creator>Zhou, Yu</creator><creator>Lu, Mei</creator><creator>Liang, Jintao</creator><creator>Zhou, Xueqing</creator><creator>Zhou, Zhide</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20250101</creationdate><title>Carbon-based light addressable potential aptasensor based on the synergy of C-MXene@rGO and OPD@NGQDs for low-density lipoprotein detection</title><author>Li, Guiyin ; Wu, Guangxiong ; Huang, Qing ; Dong, Shuaikang ; Zhou, Yu ; Lu, Mei ; Liang, Jintao ; Zhou, Xueqing ; Zhou, Zhide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-11f910bb10fa342beac9ae64a30343e1934d0a64a0d95208332610a57e4825d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Analytical Chemistry</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Biocompatibility</topic><topic>Biosensing Techniques - methods</topic><topic>Carbon</topic><topic>Carbon - chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Density</topic><topic>Electric potential</topic><topic>Electrochemical Techniques - instrumentation</topic><topic>Electrochemical Techniques - methods</topic><topic>Graphene</topic><topic>Graphite - chemistry</topic><topic>Humans</topic><topic>Light</topic><topic>Limit of Detection</topic><topic>Lipoproteins, LDL - blood</topic><topic>Lipoproteins, LDL - chemistry</topic><topic>Microengineering</topic><topic>MXenes</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Phenylenediamine</topic><topic>Phenylenediamines - chemistry</topic><topic>Photoelectricity</topic><topic>Photosensitivity</topic><topic>Quantum dots</topic><topic>Quantum Dots - chemistry</topic><topic>Response surface methodology</topic><topic>Titanium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Guiyin</creatorcontrib><creatorcontrib>Wu, Guangxiong</creatorcontrib><creatorcontrib>Huang, Qing</creatorcontrib><creatorcontrib>Dong, Shuaikang</creatorcontrib><creatorcontrib>Zhou, Yu</creatorcontrib><creatorcontrib>Lu, Mei</creatorcontrib><creatorcontrib>Liang, Jintao</creatorcontrib><creatorcontrib>Zhou, Xueqing</creatorcontrib><creatorcontrib>Zhou, Zhide</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Guiyin</au><au>Wu, Guangxiong</au><au>Huang, Qing</au><au>Dong, Shuaikang</au><au>Zhou, Yu</au><au>Lu, Mei</au><au>Liang, Jintao</au><au>Zhou, Xueqing</au><au>Zhou, Zhide</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbon-based light addressable potential aptasensor based on the synergy of C-MXene@rGO and OPD@NGQDs for low-density lipoprotein detection</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2025-01-01</date><risdate>2025</risdate><volume>192</volume><issue>1</issue><spage>35</spage><pages>35-</pages><artnum>35</artnum><issn>0026-3672</issn><issn>1436-5073</issn><eissn>1436-5073</eissn><abstract>A novel carbon-based light-addressable potentiometric aptasensor (C-LAPS) was constructed for detection low-density lipoprotein (LDL) in serum. Carboxylated Ti
3
C
2
MXene @reduced graphene oxide (C-MXene@rGO) was used as interface and o-phenylenediamine functionalized nitrogen-doped graphene quantum dots (OPD@NGQDs) as the photoelectric conversion element. The photosensitive layers composed of OPD@NGQDs/C-MXene@rGO exhibit superior photoelectric conversion efficiency and excellent biocompatibility, which contribute to an improved response signal. When LDL reacts with the LDL aptamer (LDL
Apt
) immobilized on the photosensitive layers to form LDL-LDL
Apt
complexes, the reaction process can induce the modification of the surface potential in the photosensitive layer, leading to potential shift observed through the I-V curves. The experimental conditions were successfully optimized with few planned tests by applying the Box-Behnken design and response surface methodology aspects of the Design-Expert software. Under the optimal condition, the potential shift had a linear relationship with concentrations of LDL from 0.02 to 0.30 μg/mL. The limit of detection (LOD) was 5.88 ng/mL (S/N = 3) and the sensitivity was 315.20 mV/μg·mL
−1
. In addition, the LDL C-LAPS demonstrated excellent specificity, reproducibility, and stability in detecting LDL. The sensor performed well in quantifying LDL in real samples. Therefore, the LDL C-LAPS has the potential for clinical applications.
Graphical Abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>39729216</pmid><doi>10.1007/s00604-024-06909-w</doi></addata></record> |
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| subjects | Analytical Chemistry Aptamers, Nucleotide - chemistry Biocompatibility Biosensing Techniques - methods Carbon Carbon - chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Density Electric potential Electrochemical Techniques - instrumentation Electrochemical Techniques - methods Graphene Graphite - chemistry Humans Light Limit of Detection Lipoproteins, LDL - blood Lipoproteins, LDL - chemistry Microengineering MXenes Nanochemistry Nanotechnology Phenylenediamine Phenylenediamines - chemistry Photoelectricity Photosensitivity Quantum dots Quantum Dots - chemistry Response surface methodology Titanium - chemistry |
| title | Carbon-based light addressable potential aptasensor based on the synergy of C-MXene@rGO and OPD@NGQDs for low-density lipoprotein detection |
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