Simultaneous separation and detection of common chiral and achiral metabolites in the urine of human exposed to benzene series by LC-MS/MS

•Developed novel chiral stationary phase EACDP, achieving high resolution for PMA, BMA, and MHBMA-2 enantiomers within 50 min.•Detection limits as low as 0.109 μg/L, recoveries 78–116 %, ensuring accurate quantification in complex matrices.•Applied to 60 urine samples from an auto plant, revealing s...

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