Glycosyltransferase‐Mediated Modulation of Reactive Oxygen Species Enhances Non‐host Resistance to Pst DC3000 in Nicotiana benthamiana
Non‐host resistance (NHR) governs defense responses against a broad range of potential pathogen species in contrast with host resistance. To identify specific genes involved in disease resistance, we used a virus‐induced gene‐silencing screen in Nicotiana benthamiana and identified glycosyltransfera...
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| Veröffentlicht in: | Physiologia plantarum 2024-11, Vol.176 (6), p.e70019-n/a |
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| container_title | Physiologia plantarum |
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| creator | Liu, Yingjun Zhang, Siyi Sun, Min Hao, Xingqian Jin, Pinyuan Luo, Sheng Chen, Jiao Zhang, Ting Ge, Shating Zhang, Huajian |
| description | Non‐host resistance (NHR) governs defense responses against a broad range of potential pathogen species in contrast with host resistance. To identify specific genes involved in disease resistance, we used a virus‐induced gene‐silencing screen in Nicotiana benthamiana and identified glycosyltransferase (NbGT) as an essential component of NHR. NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana, improving its NHR to Pseudomonas syringae pv. tomato (Pst) DC3000. NbGT participated in reactive oxygen species accumulation caused by flg22 rather than coronatine and HrcC of Pst DC3000. Analyses of gene expression and enzyme activity demonstrated that NbGT‐silenced plants exhibited enhanced expression and elevated levels of superoxide dismutase, resulting in heightened accumulation of H2O2. In conclusion, NbGT‐silencing increases H2O2 accumulation by regulating superoxide dismutase activity during the immune response to flg22, enhancing resistance to Pst DC3000 in N. benthamiana. This research provides novel insights into the role of glycosyltransferases in NHR. |
| doi_str_mv | 10.1111/ppl.70019 |
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To identify specific genes involved in disease resistance, we used a virus‐induced gene‐silencing screen in Nicotiana benthamiana and identified glycosyltransferase (NbGT) as an essential component of NHR. NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana, improving its NHR to Pseudomonas syringae pv. tomato (Pst) DC3000. NbGT participated in reactive oxygen species accumulation caused by flg22 rather than coronatine and HrcC of Pst DC3000. Analyses of gene expression and enzyme activity demonstrated that NbGT‐silenced plants exhibited enhanced expression and elevated levels of superoxide dismutase, resulting in heightened accumulation of H2O2. In conclusion, NbGT‐silencing increases H2O2 accumulation by regulating superoxide dismutase activity during the immune response to flg22, enhancing resistance to Pst DC3000 in N. benthamiana. This research provides novel insights into the role of glycosyltransferases in NHR.</description><identifier>ISSN: 0031-9317</identifier><identifier>ISSN: 1399-3054</identifier><identifier>EISSN: 1399-3054</identifier><identifier>DOI: 10.1111/ppl.70019</identifier><identifier>PMID: 39703073</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Accumulation ; Coronatine ; Disease resistance ; Disease Resistance - genetics ; Enzymatic activity ; Enzyme activity ; Gene expression ; Gene Expression Regulation, Plant ; Gene Silencing ; Glucans - metabolism ; Glycosyltransferase ; Glycosyltransferases - genetics ; Glycosyltransferases - metabolism ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Hypersensitivity ; Immune response ; Nicotiana - genetics ; Nicotiana - immunology ; Nicotiana - metabolism ; Nicotiana - microbiology ; Nicotiana benthamiana ; Oxygen ; Plant Diseases - genetics ; Plant Diseases - immunology ; Plant Diseases - microbiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Pseudomonas syringae - pathogenicity ; Pseudomonas syringae - physiology ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Superoxide dismutase ; Superoxide Dismutase - genetics ; Superoxide Dismutase - metabolism ; Tomatoes</subject><ispartof>Physiologia plantarum, 2024-11, Vol.176 (6), p.e70019-n/a</ispartof><rights>2024 Scandinavian Plant Physiology Society.</rights><rights>2024 Scandinavian Plant Physiology Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2439-863a00ca41906fe5006cced33450ddef2b0f760ecbf38c0efdcad2c1cc027b983</cites><orcidid>0000-0002-2445-6286</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fppl.70019$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fppl.70019$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39703073$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yingjun</creatorcontrib><creatorcontrib>Zhang, Siyi</creatorcontrib><creatorcontrib>Sun, Min</creatorcontrib><creatorcontrib>Hao, Xingqian</creatorcontrib><creatorcontrib>Jin, Pinyuan</creatorcontrib><creatorcontrib>Luo, Sheng</creatorcontrib><creatorcontrib>Chen, Jiao</creatorcontrib><creatorcontrib>Zhang, Ting</creatorcontrib><creatorcontrib>Ge, Shating</creatorcontrib><creatorcontrib>Zhang, Huajian</creatorcontrib><title>Glycosyltransferase‐Mediated Modulation of Reactive Oxygen Species Enhances Non‐host Resistance to Pst DC3000 in Nicotiana benthamiana</title><title>Physiologia plantarum</title><addtitle>Physiol Plant</addtitle><description>Non‐host resistance (NHR) governs defense responses against a broad range of potential pathogen species in contrast with host resistance. To identify specific genes involved in disease resistance, we used a virus‐induced gene‐silencing screen in Nicotiana benthamiana and identified glycosyltransferase (NbGT) as an essential component of NHR. NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana, improving its NHR to Pseudomonas syringae pv. tomato (Pst) DC3000. NbGT participated in reactive oxygen species accumulation caused by flg22 rather than coronatine and HrcC of Pst DC3000. Analyses of gene expression and enzyme activity demonstrated that NbGT‐silenced plants exhibited enhanced expression and elevated levels of superoxide dismutase, resulting in heightened accumulation of H2O2. In conclusion, NbGT‐silencing increases H2O2 accumulation by regulating superoxide dismutase activity during the immune response to flg22, enhancing resistance to Pst DC3000 in N. benthamiana. This research provides novel insights into the role of glycosyltransferases in NHR.</description><subject>Accumulation</subject><subject>Coronatine</subject><subject>Disease resistance</subject><subject>Disease Resistance - genetics</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Silencing</subject><subject>Glucans - metabolism</subject><subject>Glycosyltransferase</subject><subject>Glycosyltransferases - genetics</subject><subject>Glycosyltransferases - metabolism</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Hypersensitivity</subject><subject>Immune response</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - immunology</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana - microbiology</subject><subject>Nicotiana benthamiana</subject><subject>Oxygen</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - immunology</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Pseudomonas syringae - pathogenicity</subject><subject>Pseudomonas syringae - physiology</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Tomatoes</subject><issn>0031-9317</issn><issn>1399-3054</issn><issn>1399-3054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10UFv0zAYBmALMbGucOAPIEtc4JDtc5wm9RGVsU3qtmqDc-TYn6mn1A6xA-S28077jfslc9fBAQlfbH16_MryS8hbBocsraOuaw8rACZekAnjQmQcZsVLMgHgLBOcVfvkIISbJMqS5a_IPhcVcKj4hNydtKPyYWxjL10w2MuAD7f356itjKjpuddDK6P1jnpDr1CqaH8ivfw9fkdHrztUFgM9dmvpVDpceJdur32IyQYb4nZMo6erNPm84ABAraMXVvlopZO0QRfXcrM9vyZ7RrYB3zzvU_Lty_HXxWm2vDw5W3xaZiovuMjmJZcAShZMQGlwBlAqhZrzYgZao8kbMFUJqBrD5wrQaCV1rphSkFeNmPMp-bDL7Xr_Y8AQ640NCttWOvRDqDkrqmI-YxVP9P0_9MYPvUuv2yrB8hLSL07Jx51SvQ-hR1N3vd3IfqwZ1NuC6lRQ_VRQsu-eE4dmg_qv_NNIAkc78Mu2OP4_qV6tlrvIRx4TnXA</recordid><startdate>202411</startdate><enddate>202411</enddate><creator>Liu, Yingjun</creator><creator>Zhang, Siyi</creator><creator>Sun, Min</creator><creator>Hao, Xingqian</creator><creator>Jin, Pinyuan</creator><creator>Luo, Sheng</creator><creator>Chen, Jiao</creator><creator>Zhang, Ting</creator><creator>Ge, Shating</creator><creator>Zhang, Huajian</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2445-6286</orcidid></search><sort><creationdate>202411</creationdate><title>Glycosyltransferase‐Mediated Modulation of Reactive Oxygen Species Enhances Non‐host Resistance to Pst DC3000 in Nicotiana benthamiana</title><author>Liu, Yingjun ; Zhang, Siyi ; Sun, Min ; Hao, Xingqian ; Jin, Pinyuan ; Luo, Sheng ; Chen, Jiao ; Zhang, Ting ; Ge, Shating ; Zhang, Huajian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2439-863a00ca41906fe5006cced33450ddef2b0f760ecbf38c0efdcad2c1cc027b983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Accumulation</topic><topic>Coronatine</topic><topic>Disease resistance</topic><topic>Disease Resistance - genetics</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Silencing</topic><topic>Glucans - metabolism</topic><topic>Glycosyltransferase</topic><topic>Glycosyltransferases - genetics</topic><topic>Glycosyltransferases - metabolism</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Hypersensitivity</topic><topic>Immune response</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - immunology</topic><topic>Nicotiana - metabolism</topic><topic>Nicotiana - microbiology</topic><topic>Nicotiana benthamiana</topic><topic>Oxygen</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - immunology</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Pseudomonas syringae - pathogenicity</topic><topic>Pseudomonas syringae - physiology</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Tomatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yingjun</creatorcontrib><creatorcontrib>Zhang, Siyi</creatorcontrib><creatorcontrib>Sun, Min</creatorcontrib><creatorcontrib>Hao, Xingqian</creatorcontrib><creatorcontrib>Jin, Pinyuan</creatorcontrib><creatorcontrib>Luo, Sheng</creatorcontrib><creatorcontrib>Chen, Jiao</creatorcontrib><creatorcontrib>Zhang, Ting</creatorcontrib><creatorcontrib>Ge, Shating</creatorcontrib><creatorcontrib>Zhang, Huajian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiologia plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yingjun</au><au>Zhang, Siyi</au><au>Sun, Min</au><au>Hao, Xingqian</au><au>Jin, Pinyuan</au><au>Luo, Sheng</au><au>Chen, Jiao</au><au>Zhang, Ting</au><au>Ge, Shating</au><au>Zhang, Huajian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glycosyltransferase‐Mediated Modulation of Reactive Oxygen Species Enhances Non‐host Resistance to Pst DC3000 in Nicotiana benthamiana</atitle><jtitle>Physiologia plantarum</jtitle><addtitle>Physiol Plant</addtitle><date>2024-11</date><risdate>2024</risdate><volume>176</volume><issue>6</issue><spage>e70019</spage><epage>n/a</epage><pages>e70019-n/a</pages><issn>0031-9317</issn><issn>1399-3054</issn><eissn>1399-3054</eissn><abstract>Non‐host resistance (NHR) governs defense responses against a broad range of potential pathogen species in contrast with host resistance. To identify specific genes involved in disease resistance, we used a virus‐induced gene‐silencing screen in Nicotiana benthamiana and identified glycosyltransferase (NbGT) as an essential component of NHR. NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana, improving its NHR to Pseudomonas syringae pv. tomato (Pst) DC3000. NbGT participated in reactive oxygen species accumulation caused by flg22 rather than coronatine and HrcC of Pst DC3000. Analyses of gene expression and enzyme activity demonstrated that NbGT‐silenced plants exhibited enhanced expression and elevated levels of superoxide dismutase, resulting in heightened accumulation of H2O2. In conclusion, NbGT‐silencing increases H2O2 accumulation by regulating superoxide dismutase activity during the immune response to flg22, enhancing resistance to Pst DC3000 in N. benthamiana. This research provides novel insights into the role of glycosyltransferases in NHR.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>39703073</pmid><doi>10.1111/ppl.70019</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-2445-6286</orcidid></addata></record> |
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| subjects | Accumulation Coronatine Disease resistance Disease Resistance - genetics Enzymatic activity Enzyme activity Gene expression Gene Expression Regulation, Plant Gene Silencing Glucans - metabolism Glycosyltransferase Glycosyltransferases - genetics Glycosyltransferases - metabolism Hydrogen peroxide Hydrogen Peroxide - metabolism Hypersensitivity Immune response Nicotiana - genetics Nicotiana - immunology Nicotiana - metabolism Nicotiana - microbiology Nicotiana benthamiana Oxygen Plant Diseases - genetics Plant Diseases - immunology Plant Diseases - microbiology Plant Proteins - genetics Plant Proteins - metabolism Pseudomonas syringae - pathogenicity Pseudomonas syringae - physiology Reactive oxygen species Reactive Oxygen Species - metabolism Superoxide dismutase Superoxide Dismutase - genetics Superoxide Dismutase - metabolism Tomatoes |
| title | Glycosyltransferase‐Mediated Modulation of Reactive Oxygen Species Enhances Non‐host Resistance to Pst DC3000 in Nicotiana benthamiana |
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