Synthetic T‑Cell Receptor-like Protein Behaves as a Janus Particle in Solution
Protein engineering enables the creation of tailor-made proteins for a variety of applications. ImmTACs stand out as promising therapeutics for cancer and other treatments while also presenting unique challenges for stability, formulation, and delivery. We have shown that ImmTACs behave as Janus par...
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| Veröffentlicht in: | Journal of the American Chemical Society 2025-01, Vol.147 (1), p.247-256 |
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| container_title | Journal of the American Chemical Society |
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| creator | Sakamoto-Rablah, Emily Bye, Jordan Modak, Arghya Hooker, Andrew Uddin, Shahid McManus, Jennifer J. |
| description | Protein engineering enables the creation of tailor-made proteins for a variety of applications. ImmTACs stand out as promising therapeutics for cancer and other treatments while also presenting unique challenges for stability, formulation, and delivery. We have shown that ImmTACs behave as Janus particles in solution, leading to self-association at low concentrations, even when the average protein–protein interactions suggest that the molecule should be stable. The formation of small but stable oligomers was confirmed by static and dynamic light scattering and analytical ultracentrifugation. Modeling of the structure using AlphaFold leads to a rational explanation for this behavior, consistent with the Janus particle assembly observed for inverse patchy particles. |
| doi_str_mv | 10.1021/jacs.4c08932 |
| format | Article |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Models, Molecular Particle Size Protein Engineering Receptors, Antigen, T-Cell - chemistry Solutions |
| title | Synthetic T‑Cell Receptor-like Protein Behaves as a Janus Particle in Solution |
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