Gene Expression Dysregulation in Whole Blood of Patients with Clostridioides difficile Infection
( ) is a global threat and has significant implications for individuals and health care systems. Little is known about host molecular mechanisms and transcriptional changes in peripheral immune cells. This is the first gene expression study in whole blood from patients with infection. We took blood...
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| Veröffentlicht in: | International journal of molecular sciences 2024-11, Vol.25 (23), p.12653 |
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| container_title | International journal of molecular sciences |
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| creator | Tsakiroglou, Maria Evans, Anthony Doce-Carracedo, Alejandra Little, Margaret Hornby, Rachel Roberts, Paul Zhang, Eunice Miyajima, Fabio Pirmohamed, Munir |
| description | (
) is a global threat and has significant implications for individuals and health care systems. Little is known about host molecular mechanisms and transcriptional changes in peripheral immune cells. This is the first gene expression study in whole blood from patients with
infection. We took blood and stool samples from patients with toxigenic
infection (CDI), non-toxigenic
infection (GDH), inflammatory bowel disease (IBD), diarrhea from other causes (DC), and healthy controls (HC). We performed transcriptome-wide RNA profiling on peripheral blood to identify diarrhea common and CDI unique gene sets. Diarrhea groups upregulated innate immune responses with neutrophils at the epicenter. The common signature associated with diarrhea was non-specific and shared by various other inflammatory conditions. CDI had a unique 45 gene set reflecting the downregulation of humoral and T cell memory functions. Dysregulation of immunometabolic genes was also abundant and linked to immune cell fate during differentiation. Whole transcriptome analysis of white cells in blood from patients with toxigenic
infection showed that there is an impairment of adaptive immunity and immunometabolism. |
| doi_str_mv | 10.3390/ijms252312653 |
| format | Article |
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) is a global threat and has significant implications for individuals and health care systems. Little is known about host molecular mechanisms and transcriptional changes in peripheral immune cells. This is the first gene expression study in whole blood from patients with
infection. We took blood and stool samples from patients with toxigenic
infection (CDI), non-toxigenic
infection (GDH), inflammatory bowel disease (IBD), diarrhea from other causes (DC), and healthy controls (HC). We performed transcriptome-wide RNA profiling on peripheral blood to identify diarrhea common and CDI unique gene sets. Diarrhea groups upregulated innate immune responses with neutrophils at the epicenter. The common signature associated with diarrhea was non-specific and shared by various other inflammatory conditions. CDI had a unique 45 gene set reflecting the downregulation of humoral and T cell memory functions. Dysregulation of immunometabolic genes was also abundant and linked to immune cell fate during differentiation. Whole transcriptome analysis of white cells in blood from patients with toxigenic
infection showed that there is an impairment of adaptive immunity and immunometabolism.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms252312653</identifier><identifier>PMID: 39684365</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adult ; Aged ; Antibiotics ; Asymptomatic ; Cardiovascular disease ; Clinical trials ; Clostridioides difficile - genetics ; Clostridium Infections - blood ; Clostridium Infections - genetics ; Clostridium Infections - immunology ; Clostridium Infections - microbiology ; Coronaviruses ; Diarrhea ; Diarrhea - blood ; Diarrhea - genetics ; Diarrhea - microbiology ; Disease prevention ; Female ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation ; Health care ; Humans ; Illnesses ; Immunity (Disease) ; Immunity, Innate - genetics ; Inflammatory bowel disease ; Male ; Microbiota ; Middle Aged ; Mortality ; Neutrophils ; Nosocomial infections ; Sepsis ; Toxins ; Transcriptome</subject><ispartof>International journal of molecular sciences, 2024-11, Vol.25 (23), p.12653</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-8547-1730 ; 0009-0000-1502-0184 ; 0000-0002-7534-7266 ; 0000-0001-7946-3953</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39684365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsakiroglou, Maria</creatorcontrib><creatorcontrib>Evans, Anthony</creatorcontrib><creatorcontrib>Doce-Carracedo, Alejandra</creatorcontrib><creatorcontrib>Little, Margaret</creatorcontrib><creatorcontrib>Hornby, Rachel</creatorcontrib><creatorcontrib>Roberts, Paul</creatorcontrib><creatorcontrib>Zhang, Eunice</creatorcontrib><creatorcontrib>Miyajima, Fabio</creatorcontrib><creatorcontrib>Pirmohamed, Munir</creatorcontrib><title>Gene Expression Dysregulation in Whole Blood of Patients with Clostridioides difficile Infection</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>(
) is a global threat and has significant implications for individuals and health care systems. Little is known about host molecular mechanisms and transcriptional changes in peripheral immune cells. This is the first gene expression study in whole blood from patients with
infection. We took blood and stool samples from patients with toxigenic
infection (CDI), non-toxigenic
infection (GDH), inflammatory bowel disease (IBD), diarrhea from other causes (DC), and healthy controls (HC). We performed transcriptome-wide RNA profiling on peripheral blood to identify diarrhea common and CDI unique gene sets. Diarrhea groups upregulated innate immune responses with neutrophils at the epicenter. The common signature associated with diarrhea was non-specific and shared by various other inflammatory conditions. CDI had a unique 45 gene set reflecting the downregulation of humoral and T cell memory functions. Dysregulation of immunometabolic genes was also abundant and linked to immune cell fate during differentiation. Whole transcriptome analysis of white cells in blood from patients with toxigenic
infection showed that there is an impairment of adaptive immunity and immunometabolism.</description><subject>Adult</subject><subject>Aged</subject><subject>Antibiotics</subject><subject>Asymptomatic</subject><subject>Cardiovascular disease</subject><subject>Clinical trials</subject><subject>Clostridioides difficile - genetics</subject><subject>Clostridium Infections - blood</subject><subject>Clostridium Infections - genetics</subject><subject>Clostridium Infections - immunology</subject><subject>Clostridium Infections - microbiology</subject><subject>Coronaviruses</subject><subject>Diarrhea</subject><subject>Diarrhea - blood</subject><subject>Diarrhea - genetics</subject><subject>Diarrhea - microbiology</subject><subject>Disease prevention</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Health care</subject><subject>Humans</subject><subject>Illnesses</subject><subject>Immunity (Disease)</subject><subject>Immunity, Innate - genetics</subject><subject>Inflammatory bowel disease</subject><subject>Male</subject><subject>Microbiota</subject><subject>Middle Aged</subject><subject>Mortality</subject><subject>Neutrophils</subject><subject>Nosocomial infections</subject><subject>Sepsis</subject><subject>Toxins</subject><subject>Transcriptome</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkD1PwzAQhi0EoqUwsiJLLCwBfycZoZRSqRIMIMaQ-oO6SuJiJ4L-exy1IGA43Vl-9OruAeAUo0tKc3RlV3UgnFBMBKd7YIgZIQlCIt3_NQ_AUQgrhAglPD8EA5qLjFHBh-B1qhsNJ59rr0OwroG3m-D1W1eVbf-yDXxZukrDm8o5BZ2Bj_FDN22AH7ZdwnHlQuutss4qHaCyxlhpIz9rjJZ9xDE4MGUV9Mmuj8Dz3eRpfJ_MH6az8fU8kVhgmgiJaZYagSUVCCmD8nIhCSMl1tywFCtsBM_QAinEU54iU2Y6Xsc55ZLoVNERuNjmrr1773Roi9oGqauqbLTrQkExE3ksxCJ6_g9duc43cbueYjhPGaWRSraU9C5EJ6ZYe1uXflNgVPTqiz_qI3-2S-0WtVY_9Ldr-gU17n5p</recordid><startdate>20241125</startdate><enddate>20241125</enddate><creator>Tsakiroglou, Maria</creator><creator>Evans, Anthony</creator><creator>Doce-Carracedo, Alejandra</creator><creator>Little, Margaret</creator><creator>Hornby, Rachel</creator><creator>Roberts, Paul</creator><creator>Zhang, Eunice</creator><creator>Miyajima, Fabio</creator><creator>Pirmohamed, Munir</creator><general>MDPI AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8547-1730</orcidid><orcidid>https://orcid.org/0009-0000-1502-0184</orcidid><orcidid>https://orcid.org/0000-0002-7534-7266</orcidid><orcidid>https://orcid.org/0000-0001-7946-3953</orcidid></search><sort><creationdate>20241125</creationdate><title>Gene Expression Dysregulation in Whole Blood of Patients with Clostridioides difficile Infection</title><author>Tsakiroglou, Maria ; 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) is a global threat and has significant implications for individuals and health care systems. Little is known about host molecular mechanisms and transcriptional changes in peripheral immune cells. This is the first gene expression study in whole blood from patients with
infection. We took blood and stool samples from patients with toxigenic
infection (CDI), non-toxigenic
infection (GDH), inflammatory bowel disease (IBD), diarrhea from other causes (DC), and healthy controls (HC). We performed transcriptome-wide RNA profiling on peripheral blood to identify diarrhea common and CDI unique gene sets. Diarrhea groups upregulated innate immune responses with neutrophils at the epicenter. The common signature associated with diarrhea was non-specific and shared by various other inflammatory conditions. CDI had a unique 45 gene set reflecting the downregulation of humoral and T cell memory functions. Dysregulation of immunometabolic genes was also abundant and linked to immune cell fate during differentiation. Whole transcriptome analysis of white cells in blood from patients with toxigenic
infection showed that there is an impairment of adaptive immunity and immunometabolism.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39684365</pmid><doi>10.3390/ijms252312653</doi><orcidid>https://orcid.org/0000-0001-8547-1730</orcidid><orcidid>https://orcid.org/0009-0000-1502-0184</orcidid><orcidid>https://orcid.org/0000-0002-7534-7266</orcidid><orcidid>https://orcid.org/0000-0001-7946-3953</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adult Aged Antibiotics Asymptomatic Cardiovascular disease Clinical trials Clostridioides difficile - genetics Clostridium Infections - blood Clostridium Infections - genetics Clostridium Infections - immunology Clostridium Infections - microbiology Coronaviruses Diarrhea Diarrhea - blood Diarrhea - genetics Diarrhea - microbiology Disease prevention Female Gene expression Gene Expression Profiling Gene Expression Regulation Health care Humans Illnesses Immunity (Disease) Immunity, Innate - genetics Inflammatory bowel disease Male Microbiota Middle Aged Mortality Neutrophils Nosocomial infections Sepsis Toxins Transcriptome |
| title | Gene Expression Dysregulation in Whole Blood of Patients with Clostridioides difficile Infection |
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