Extracellular hydrolytic enzyme activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus with oropharyngeal candidiasis at HIV/AIDS clinics in Uganda
Commensal oral Candida species can become opportunistic and transition to pathogenic causes of oropharyngeal candidiasis (OPC) in individuals with impaired immunity through ecological cues and the expression of extracellular hydrolytic enzyme activities and biofilm formation. We evaluated phospholip...
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| Veröffentlicht in: | Microbial pathogenesis 2024-12, Vol.199, p.107232, Article 107232 |
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| creator | Musinguzi, Benson Akampurira, Andrew Derick, Hope Turyamuhika, Laban Mwesigwa, Alex Mwebesa, Edson Mwesigye, Vicent Kabajulizi, Immaculate Sekulima, Tahalu Ocheng, Francis Itabangi, Herbert Mboowa, Gerald Sande, Obondo James Achan, Beatrice |
| description | Commensal oral Candida species can become opportunistic and transition to pathogenic causes of oropharyngeal candidiasis (OPC) in individuals with impaired immunity through ecological cues and the expression of extracellular hydrolytic enzyme activities and biofilm formation.
We evaluated phospholipase, proteinase, hemolysin, esterase, and coagulase enzymatic activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus (PLHIV) with OPC.
Thirty-five Candida isolates from PLHIV with OPC were retrieved from a sample repository and evaluated for phospholipase activity using the egg yolk agar method, proteinase activity using the bovine serum albumin agar method, hemolysin activity using the blood agar plate method, esterase activity using the Tween 80 opacity test medium method, coagulase activity using the classical tube method, and biofilm formation using the microtiter plate assay method in vitro.
A total of 35 Candida isolates obtained from PLHIV with OPC were included in this study, and phospholipase and proteinase activities were detected in 33/35 (94.3 %) and 31/35 (88.6 %) Candida isolates, respectively. Up to 25/35 (71.4 %) of the Candida isolates exhibited biofilm formation, whereas esterase activity was demonstrated in 23/35 (65.7 %) of the Candida isolates. Fewer isolates (21/35, 60 %) produced hemolysin, and coagulase production was the least common virulence activity detected in 18/35 (51.4 %) of the Candida isolates.
Phospholipase and proteinase activities were the strongest in oropharyngeal Candida species.
•Candida pathogens secrete hydrolytic enzymes and form biofilms during pathogenesis.•Phospholipase activity was detected in 100 % of Candida albicans.•Proteinase activity was observed in 88.6 % of Candida isolates.•Biofilm formation was noted in 71.4 % of Candida isolates.•Biofilm formation was detected in 65 % of the Candida albicans. |
| doi_str_mv | 10.1016/j.micpath.2024.107232 |
| format | Article |
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We evaluated phospholipase, proteinase, hemolysin, esterase, and coagulase enzymatic activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus (PLHIV) with OPC.
Thirty-five Candida isolates from PLHIV with OPC were retrieved from a sample repository and evaluated for phospholipase activity using the egg yolk agar method, proteinase activity using the bovine serum albumin agar method, hemolysin activity using the blood agar plate method, esterase activity using the Tween 80 opacity test medium method, coagulase activity using the classical tube method, and biofilm formation using the microtiter plate assay method in vitro.
A total of 35 Candida isolates obtained from PLHIV with OPC were included in this study, and phospholipase and proteinase activities were detected in 33/35 (94.3 %) and 31/35 (88.6 %) Candida isolates, respectively. Up to 25/35 (71.4 %) of the Candida isolates exhibited biofilm formation, whereas esterase activity was demonstrated in 23/35 (65.7 %) of the Candida isolates. Fewer isolates (21/35, 60 %) produced hemolysin, and coagulase production was the least common virulence activity detected in 18/35 (51.4 %) of the Candida isolates.
Phospholipase and proteinase activities were the strongest in oropharyngeal Candida species.
•Candida pathogens secrete hydrolytic enzymes and form biofilms during pathogenesis.•Phospholipase activity was detected in 100 % of Candida albicans.•Proteinase activity was observed in 88.6 % of Candida isolates.•Biofilm formation was noted in 71.4 % of Candida isolates.•Biofilm formation was detected in 65 % of the Candida albicans.</description><identifier>ISSN: 0882-4010</identifier><identifier>ISSN: 1096-1208</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2024.107232</identifier><identifier>PMID: 39675440</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Biofilm ; Coagulase ; Esterase ; Hemolysin ; Phospholipase ; Proteinase</subject><ispartof>Microbial pathogenesis, 2024-12, Vol.199, p.107232, Article 107232</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-9741-5429 ; 0000-0002-5230-6189 ; 0009-0004-4630-5800 ; 0000-0003-3010-2537 ; 0000-0001-8445-9414 ; 0000-0003-4421-5356 ; 0000-0002-1211-4617</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2024.107232$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39675440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Musinguzi, Benson</creatorcontrib><creatorcontrib>Akampurira, Andrew</creatorcontrib><creatorcontrib>Derick, Hope</creatorcontrib><creatorcontrib>Turyamuhika, Laban</creatorcontrib><creatorcontrib>Mwesigwa, Alex</creatorcontrib><creatorcontrib>Mwebesa, Edson</creatorcontrib><creatorcontrib>Mwesigye, Vicent</creatorcontrib><creatorcontrib>Kabajulizi, Immaculate</creatorcontrib><creatorcontrib>Sekulima, Tahalu</creatorcontrib><creatorcontrib>Ocheng, Francis</creatorcontrib><creatorcontrib>Itabangi, Herbert</creatorcontrib><creatorcontrib>Mboowa, Gerald</creatorcontrib><creatorcontrib>Sande, Obondo James</creatorcontrib><creatorcontrib>Achan, Beatrice</creatorcontrib><title>Extracellular hydrolytic enzyme activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus with oropharyngeal candidiasis at HIV/AIDS clinics in Uganda</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Commensal oral Candida species can become opportunistic and transition to pathogenic causes of oropharyngeal candidiasis (OPC) in individuals with impaired immunity through ecological cues and the expression of extracellular hydrolytic enzyme activities and biofilm formation.
We evaluated phospholipase, proteinase, hemolysin, esterase, and coagulase enzymatic activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus (PLHIV) with OPC.
Thirty-five Candida isolates from PLHIV with OPC were retrieved from a sample repository and evaluated for phospholipase activity using the egg yolk agar method, proteinase activity using the bovine serum albumin agar method, hemolysin activity using the blood agar plate method, esterase activity using the Tween 80 opacity test medium method, coagulase activity using the classical tube method, and biofilm formation using the microtiter plate assay method in vitro.
A total of 35 Candida isolates obtained from PLHIV with OPC were included in this study, and phospholipase and proteinase activities were detected in 33/35 (94.3 %) and 31/35 (88.6 %) Candida isolates, respectively. Up to 25/35 (71.4 %) of the Candida isolates exhibited biofilm formation, whereas esterase activity was demonstrated in 23/35 (65.7 %) of the Candida isolates. Fewer isolates (21/35, 60 %) produced hemolysin, and coagulase production was the least common virulence activity detected in 18/35 (51.4 %) of the Candida isolates.
Phospholipase and proteinase activities were the strongest in oropharyngeal Candida species.
•Candida pathogens secrete hydrolytic enzymes and form biofilms during pathogenesis.•Phospholipase activity was detected in 100 % of Candida albicans.•Proteinase activity was observed in 88.6 % of Candida isolates.•Biofilm formation was noted in 71.4 % of Candida isolates.•Biofilm formation was detected in 65 % of the Candida albicans.</description><subject>Biofilm</subject><subject>Coagulase</subject><subject>Esterase</subject><subject>Hemolysin</subject><subject>Phospholipase</subject><subject>Proteinase</subject><issn>0882-4010</issn><issn>1096-1208</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNo1kc9uEzEQxlcIREPhEUA-cklqe73_TqhKC41UiQOUq-XYs8lE9nqxvYHwkDwT3iacLI1_M_PN9xXFe0ZXjLL65rByqEeV9itOuci1hpf8RbFgtKuXjNP2ZbGgbcuXgjJ6VbyJ8UAp7UTZvS6uyq5uKiHoovh7_zsFpcHayapA9icTvD0l1ASGPycHROmER0wIkajBkC36Hq0jvQ9OJfQDwYGs8w8aReIIegYxeqsSGNIH78gIfrRAbB4z7MgvTHuyn5zKnc5NgzfQY-4a9IkcMUzxTPjgx70Kp2EHyhL9vABVxKwikYfNj5vbzd03oi0OqOOs4WmXGfW2eNUrG-Hd5b0unj7ff18_LB-_ftmsbx-XwLI3y22t6gp4w1ilqBINVKKuFfQdCM0YbY1p-x6UqA1rORW06htomOC05A3vuCivi4_nuWPwPyeISTqMs4tqAD9FWTJRt6IVZZvRDxd02jowcgzo8mHyfwYZ-HQGIAs-IgQZn_0AgwF0ksajZFTOqcuDvKQu59TlOfXyH--OpYc</recordid><startdate>20241214</startdate><enddate>20241214</enddate><creator>Musinguzi, Benson</creator><creator>Akampurira, Andrew</creator><creator>Derick, Hope</creator><creator>Turyamuhika, Laban</creator><creator>Mwesigwa, Alex</creator><creator>Mwebesa, Edson</creator><creator>Mwesigye, Vicent</creator><creator>Kabajulizi, Immaculate</creator><creator>Sekulima, Tahalu</creator><creator>Ocheng, Francis</creator><creator>Itabangi, Herbert</creator><creator>Mboowa, Gerald</creator><creator>Sande, Obondo James</creator><creator>Achan, Beatrice</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9741-5429</orcidid><orcidid>https://orcid.org/0000-0002-5230-6189</orcidid><orcidid>https://orcid.org/0009-0004-4630-5800</orcidid><orcidid>https://orcid.org/0000-0003-3010-2537</orcidid><orcidid>https://orcid.org/0000-0001-8445-9414</orcidid><orcidid>https://orcid.org/0000-0003-4421-5356</orcidid><orcidid>https://orcid.org/0000-0002-1211-4617</orcidid></search><sort><creationdate>20241214</creationdate><title>Extracellular hydrolytic enzyme activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus with oropharyngeal candidiasis at HIV/AIDS clinics in Uganda</title><author>Musinguzi, Benson ; Akampurira, Andrew ; Derick, Hope ; Turyamuhika, Laban ; Mwesigwa, Alex ; Mwebesa, Edson ; Mwesigye, Vicent ; Kabajulizi, Immaculate ; Sekulima, Tahalu ; Ocheng, Francis ; Itabangi, Herbert ; Mboowa, Gerald ; Sande, Obondo James ; Achan, Beatrice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1120-b6a65e27115a0a47e5466aef9e4c1108dd8ffea46d1820405f7e7142032729243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biofilm</topic><topic>Coagulase</topic><topic>Esterase</topic><topic>Hemolysin</topic><topic>Phospholipase</topic><topic>Proteinase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Musinguzi, Benson</creatorcontrib><creatorcontrib>Akampurira, Andrew</creatorcontrib><creatorcontrib>Derick, Hope</creatorcontrib><creatorcontrib>Turyamuhika, Laban</creatorcontrib><creatorcontrib>Mwesigwa, Alex</creatorcontrib><creatorcontrib>Mwebesa, Edson</creatorcontrib><creatorcontrib>Mwesigye, Vicent</creatorcontrib><creatorcontrib>Kabajulizi, Immaculate</creatorcontrib><creatorcontrib>Sekulima, Tahalu</creatorcontrib><creatorcontrib>Ocheng, Francis</creatorcontrib><creatorcontrib>Itabangi, Herbert</creatorcontrib><creatorcontrib>Mboowa, Gerald</creatorcontrib><creatorcontrib>Sande, Obondo James</creatorcontrib><creatorcontrib>Achan, Beatrice</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Musinguzi, Benson</au><au>Akampurira, Andrew</au><au>Derick, Hope</au><au>Turyamuhika, Laban</au><au>Mwesigwa, Alex</au><au>Mwebesa, Edson</au><au>Mwesigye, Vicent</au><au>Kabajulizi, Immaculate</au><au>Sekulima, Tahalu</au><au>Ocheng, Francis</au><au>Itabangi, Herbert</au><au>Mboowa, Gerald</au><au>Sande, Obondo James</au><au>Achan, Beatrice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extracellular hydrolytic enzyme activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus with oropharyngeal candidiasis at HIV/AIDS clinics in Uganda</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2024-12-14</date><risdate>2024</risdate><volume>199</volume><spage>107232</spage><pages>107232-</pages><artnum>107232</artnum><issn>0882-4010</issn><issn>1096-1208</issn><eissn>1096-1208</eissn><abstract>Commensal oral Candida species can become opportunistic and transition to pathogenic causes of oropharyngeal candidiasis (OPC) in individuals with impaired immunity through ecological cues and the expression of extracellular hydrolytic enzyme activities and biofilm formation.
We evaluated phospholipase, proteinase, hemolysin, esterase, and coagulase enzymatic activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus (PLHIV) with OPC.
Thirty-five Candida isolates from PLHIV with OPC were retrieved from a sample repository and evaluated for phospholipase activity using the egg yolk agar method, proteinase activity using the bovine serum albumin agar method, hemolysin activity using the blood agar plate method, esterase activity using the Tween 80 opacity test medium method, coagulase activity using the classical tube method, and biofilm formation using the microtiter plate assay method in vitro.
A total of 35 Candida isolates obtained from PLHIV with OPC were included in this study, and phospholipase and proteinase activities were detected in 33/35 (94.3 %) and 31/35 (88.6 %) Candida isolates, respectively. Up to 25/35 (71.4 %) of the Candida isolates exhibited biofilm formation, whereas esterase activity was demonstrated in 23/35 (65.7 %) of the Candida isolates. Fewer isolates (21/35, 60 %) produced hemolysin, and coagulase production was the least common virulence activity detected in 18/35 (51.4 %) of the Candida isolates.
Phospholipase and proteinase activities were the strongest in oropharyngeal Candida species.
•Candida pathogens secrete hydrolytic enzymes and form biofilms during pathogenesis.•Phospholipase activity was detected in 100 % of Candida albicans.•Proteinase activity was observed in 88.6 % of Candida isolates.•Biofilm formation was noted in 71.4 % of Candida isolates.•Biofilm formation was detected in 65 % of the Candida albicans.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>39675440</pmid><doi>10.1016/j.micpath.2024.107232</doi><orcidid>https://orcid.org/0000-0001-9741-5429</orcidid><orcidid>https://orcid.org/0000-0002-5230-6189</orcidid><orcidid>https://orcid.org/0009-0004-4630-5800</orcidid><orcidid>https://orcid.org/0000-0003-3010-2537</orcidid><orcidid>https://orcid.org/0000-0001-8445-9414</orcidid><orcidid>https://orcid.org/0000-0003-4421-5356</orcidid><orcidid>https://orcid.org/0000-0002-1211-4617</orcidid></addata></record> |
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| issn | 0882-4010 1096-1208 1096-1208 |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Biofilm Coagulase Esterase Hemolysin Phospholipase Proteinase |
| title | Extracellular hydrolytic enzyme activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus with oropharyngeal candidiasis at HIV/AIDS clinics in Uganda |
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