Multi-conflict islands are a widespread trend within Serratia spp
Bacteria carry numerous anti-phage systems in “defense islands” or hotspots. Recent studies have delineated the content and boundaries of these islands in various species, revealing instances of islands that encode additional factors, including antibiotic resistance genes, stress genes, type VI secr...
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creator | Cummins, Thomas Songra, Suraj Garrett, Stephen R. Blower, Tim R. Mariano, Giuseppina |
description | Bacteria carry numerous anti-phage systems in “defense islands” or hotspots. Recent studies have delineated the content and boundaries of these islands in various species, revealing instances of islands that encode additional factors, including antibiotic resistance genes, stress genes, type VI secretion system (T6SS)-dependent effectors, and virulence factors. Our study identifies three defense islands in the Serratia genus with a mixed cargo of anti-phage systems, virulence factors, and different types of anti-bacterial modules, revealing a widespread trend of co-accumulation that extends beyond T6SS-dependent effectors to colicins and contact-dependent inhibition systems. We further report the identification of four distinct anti-phage system/subtypes, including a previously unreported Toll/interleukin (IL)-1 receptor (TIR)-domain-containing system with population-wide immunity, and two loci co-opting a predicted T6SS-related protein for phage defense. This study enhances our understanding of the protein domains that can be co-opted for phage defense, resulting in a highly diversified anti-phage arsenal.
[Display omitted]
•Three Serratia islands colocalize anti-phage systems with anti-bacterial and virulence factors•This study shows genus-focused studies help uncover previously undiscovered anti-phage systems•SDIC1 combines TIR and ubiquitin ligase, uncovering toxin inhibition through ubiquitination•SDIC4 employs a VasI-like domain to reduce phage adsorption across two distinct subtypes
Cummins, Songra et al. identify three Serratia defense islands co-localizing anti-phage systems, virulence factors, and anti-bacterial factors. These islands provide a resource for discovering previously unreported systems, including four newly identified anti-phage systems (SDIC1–4). Notably, SDIC1 and SDIC4 exhibit distinct mechanisms, with SDIC1 using ubiquitination to inhibit its toxin in a TA-like manner. |
doi_str_mv | 10.1016/j.celrep.2024.115055 |
format | Article |
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[Display omitted]
•Three Serratia islands colocalize anti-phage systems with anti-bacterial and virulence factors•This study shows genus-focused studies help uncover previously undiscovered anti-phage systems•SDIC1 combines TIR and ubiquitin ligase, uncovering toxin inhibition through ubiquitination•SDIC4 employs a VasI-like domain to reduce phage adsorption across two distinct subtypes
Cummins, Songra et al. identify three Serratia defense islands co-localizing anti-phage systems, virulence factors, and anti-bacterial factors. These islands provide a resource for discovering previously unreported systems, including four newly identified anti-phage systems (SDIC1–4). Notably, SDIC1 and SDIC4 exhibit distinct mechanisms, with SDIC1 using ubiquitination to inhibit its toxin in a TA-like manner.</description><identifier>ISSN: 2211-1247</identifier><identifier>EISSN: 2211-1247</identifier><identifier>DOI: 10.1016/j.celrep.2024.115055</identifier><identifier>PMID: 39661517</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>anti-page systems discovery ; anti-phage defense ; bacterial immunity ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriophages - genetics ; defense islands ; Genomic Islands - genetics ; population-wide immunity ; Serratia ; Serratia - genetics ; Serratia - pathogenicity ; TIR ; Type VI Secretion Systems - genetics ; Type VI Secretion Systems - metabolism ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>Cell reports (Cambridge), 2024-12, Vol.43 (12), p.115055, Article 115055</ispartof><rights>2024 The Author(s)</rights><rights>Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1567-93f2d6fa1744aa7b990159a30afec30b08254b314a970a44cd03fcf56cb00a7c3</cites><orcidid>0000-0003-1197-2123</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39661517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cummins, Thomas</creatorcontrib><creatorcontrib>Songra, Suraj</creatorcontrib><creatorcontrib>Garrett, Stephen R.</creatorcontrib><creatorcontrib>Blower, Tim R.</creatorcontrib><creatorcontrib>Mariano, Giuseppina</creatorcontrib><title>Multi-conflict islands are a widespread trend within Serratia spp</title><title>Cell reports (Cambridge)</title><addtitle>Cell Rep</addtitle><description>Bacteria carry numerous anti-phage systems in “defense islands” or hotspots. Recent studies have delineated the content and boundaries of these islands in various species, revealing instances of islands that encode additional factors, including antibiotic resistance genes, stress genes, type VI secretion system (T6SS)-dependent effectors, and virulence factors. Our study identifies three defense islands in the Serratia genus with a mixed cargo of anti-phage systems, virulence factors, and different types of anti-bacterial modules, revealing a widespread trend of co-accumulation that extends beyond T6SS-dependent effectors to colicins and contact-dependent inhibition systems. We further report the identification of four distinct anti-phage system/subtypes, including a previously unreported Toll/interleukin (IL)-1 receptor (TIR)-domain-containing system with population-wide immunity, and two loci co-opting a predicted T6SS-related protein for phage defense. This study enhances our understanding of the protein domains that can be co-opted for phage defense, resulting in a highly diversified anti-phage arsenal.
[Display omitted]
•Three Serratia islands colocalize anti-phage systems with anti-bacterial and virulence factors•This study shows genus-focused studies help uncover previously undiscovered anti-phage systems•SDIC1 combines TIR and ubiquitin ligase, uncovering toxin inhibition through ubiquitination•SDIC4 employs a VasI-like domain to reduce phage adsorption across two distinct subtypes
Cummins, Songra et al. identify three Serratia defense islands co-localizing anti-phage systems, virulence factors, and anti-bacterial factors. These islands provide a resource for discovering previously unreported systems, including four newly identified anti-phage systems (SDIC1–4). Notably, SDIC1 and SDIC4 exhibit distinct mechanisms, with SDIC1 using ubiquitination to inhibit its toxin in a TA-like manner.</description><subject>anti-page systems discovery</subject><subject>anti-phage defense</subject><subject>bacterial immunity</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriophages - genetics</subject><subject>defense islands</subject><subject>Genomic Islands - genetics</subject><subject>population-wide immunity</subject><subject>Serratia</subject><subject>Serratia - genetics</subject><subject>Serratia - pathogenicity</subject><subject>TIR</subject><subject>Type VI Secretion Systems - genetics</subject><subject>Type VI Secretion Systems - metabolism</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>2211-1247</issn><issn>2211-1247</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo7rLuPxDp0UvXTJuk24uwLH7Bigf1HNJkglm6bU1axX9vlq7iybnMMLzvfDyEnANdAAVxtV1orD12i4xmbAHAKedHZJplAClkrDj-U0_IPIQtjSEoQMlOySQvhQAOxZSsHoe6d6luG1s73Scu1KoxIVEeE5V8OoOh86hM0ntsTGz0b65JntF71TuVhK47IydW1QHnhzwjr7c3L-v7dPN097BebVINXBRpmdvMCKugYEypoipLCrxUOVUWdU4rusw4q3JgqiyoYkwbmlttudAVparQ-YxcjnM7374PGHq5cyFSiPdiOwQZrUJwWNIiStko1b4NwaOVnXc75b8kULnnJ7dy5Cf3_OTIL9ouDhuGaofm1_RDKwquRwHGPz8cehm0w0ajcR51L03r_t_wDdCagaA</recordid><startdate>20241224</startdate><enddate>20241224</enddate><creator>Cummins, Thomas</creator><creator>Songra, Suraj</creator><creator>Garrett, Stephen R.</creator><creator>Blower, Tim R.</creator><creator>Mariano, Giuseppina</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1197-2123</orcidid></search><sort><creationdate>20241224</creationdate><title>Multi-conflict islands are a widespread trend within Serratia spp</title><author>Cummins, Thomas ; Songra, Suraj ; Garrett, Stephen R. ; Blower, Tim R. ; Mariano, Giuseppina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1567-93f2d6fa1744aa7b990159a30afec30b08254b314a970a44cd03fcf56cb00a7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>anti-page systems discovery</topic><topic>anti-phage defense</topic><topic>bacterial immunity</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriophages - genetics</topic><topic>defense islands</topic><topic>Genomic Islands - genetics</topic><topic>population-wide immunity</topic><topic>Serratia</topic><topic>Serratia - genetics</topic><topic>Serratia - pathogenicity</topic><topic>TIR</topic><topic>Type VI Secretion Systems - genetics</topic><topic>Type VI Secretion Systems - metabolism</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cummins, Thomas</creatorcontrib><creatorcontrib>Songra, Suraj</creatorcontrib><creatorcontrib>Garrett, Stephen R.</creatorcontrib><creatorcontrib>Blower, Tim R.</creatorcontrib><creatorcontrib>Mariano, Giuseppina</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell reports (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cummins, Thomas</au><au>Songra, Suraj</au><au>Garrett, Stephen R.</au><au>Blower, Tim R.</au><au>Mariano, Giuseppina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multi-conflict islands are a widespread trend within Serratia spp</atitle><jtitle>Cell reports (Cambridge)</jtitle><addtitle>Cell Rep</addtitle><date>2024-12-24</date><risdate>2024</risdate><volume>43</volume><issue>12</issue><spage>115055</spage><pages>115055-</pages><artnum>115055</artnum><issn>2211-1247</issn><eissn>2211-1247</eissn><abstract>Bacteria carry numerous anti-phage systems in “defense islands” or hotspots. Recent studies have delineated the content and boundaries of these islands in various species, revealing instances of islands that encode additional factors, including antibiotic resistance genes, stress genes, type VI secretion system (T6SS)-dependent effectors, and virulence factors. Our study identifies three defense islands in the Serratia genus with a mixed cargo of anti-phage systems, virulence factors, and different types of anti-bacterial modules, revealing a widespread trend of co-accumulation that extends beyond T6SS-dependent effectors to colicins and contact-dependent inhibition systems. We further report the identification of four distinct anti-phage system/subtypes, including a previously unreported Toll/interleukin (IL)-1 receptor (TIR)-domain-containing system with population-wide immunity, and two loci co-opting a predicted T6SS-related protein for phage defense. This study enhances our understanding of the protein domains that can be co-opted for phage defense, resulting in a highly diversified anti-phage arsenal.
[Display omitted]
•Three Serratia islands colocalize anti-phage systems with anti-bacterial and virulence factors•This study shows genus-focused studies help uncover previously undiscovered anti-phage systems•SDIC1 combines TIR and ubiquitin ligase, uncovering toxin inhibition through ubiquitination•SDIC4 employs a VasI-like domain to reduce phage adsorption across two distinct subtypes
Cummins, Songra et al. identify three Serratia defense islands co-localizing anti-phage systems, virulence factors, and anti-bacterial factors. These islands provide a resource for discovering previously unreported systems, including four newly identified anti-phage systems (SDIC1–4). Notably, SDIC1 and SDIC4 exhibit distinct mechanisms, with SDIC1 using ubiquitination to inhibit its toxin in a TA-like manner.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>39661517</pmid><doi>10.1016/j.celrep.2024.115055</doi><orcidid>https://orcid.org/0000-0003-1197-2123</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | anti-page systems discovery anti-phage defense bacterial immunity Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriophages - genetics defense islands Genomic Islands - genetics population-wide immunity Serratia Serratia - genetics Serratia - pathogenicity TIR Type VI Secretion Systems - genetics Type VI Secretion Systems - metabolism Virulence Factors - genetics Virulence Factors - metabolism |
title | Multi-conflict islands are a widespread trend within Serratia spp |
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