A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands

By using a limited-proteolysis strategy that employs a large amount of trypsin to generate peptides directly from native proteins, we found that ligand-induced protein local stability shifts can be sensitively detected on a proteome-wide scale. This enabled us to develop the peptide-centric local st...

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Veröffentlicht in:	Nature methods 2024-12
Hauptverfasser:	Li, Kejia, Chen, Shijie, Wang, Keyun, Wang, Yan, Xue, Lianji, Ye, Yuying, Fang, Zheng, Lyu, Jiawen, Zhu, Haiyang, Li, Yanan, Yu, Ting, Yang, Feng, Zhang, Xiaolei, Guo, Siqi, Ruan, Chengfei, Zhou, Jiahua, Wang, Qi, Dong, Mingming, Luo, Cheng, Ye, Mingliang
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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