Electrochemical immunosensing of Crohn’s disease biomarkers using diazonium salt-grafted crystalline nanocellulose/carbon nanotube-modified electrodes
The first dual immunosensor is reported for the determination of IL-12 and IL-23, two relevant biomarkers of Crohn’s disease (CD). The strategy relies on the selective capture of the targets by the respective antibodies which were covalently immobilized onto SPCEs modified with crystalline nanocellu...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2025-01, Vol.192 (1), p.4, Article 4 |
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| creator | García-Rodrigo, Lorena Ramos-López, Claudia Sánchez-Tirado, Esther Agüí, Lourdes González-Cortés, Araceli Yáñez-Sedeño, Paloma Pingarrón, José M. |
| description | The first dual immunosensor is reported for the determination of IL-12 and IL-23, two relevant biomarkers of Crohn’s disease (CD). The strategy relies on the selective capture of the targets by the respective antibodies which were covalently immobilized onto SPCEs modified with crystalline nanocellulose (CNC) and multi-walled carbon nanotubes (MWCNTs) followed by conjugation with a detector antibody labelled with poly-HRP-Strept and amperometric transduction using the H
2
O
2
/HQ system. The combination of CNC, a nanomaterial scarcely exploited in immunosensing, with MWCNTs enables the preparation of a novel dual immunosensor for the determination of CD biomarkers in clinical samples, including faeces. The developed bioplatform exhibits a high reproducibility and selectivity allowing the simultaneous determination of IL-12 and IL-23 using a smaller sample volume and in a much shorter assay time than those reported for each target with commercial ELISA kits. The dual immunosensor was successfully applied to the analysis of raw serum and faeces spiked with IL-12 and IL-23 at the expected levels in patients with severe CD.
Graphical Abstract |
| doi_str_mv | 10.1007/s00604-024-06868-2 |
| format | Article |
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2
O
2
/HQ system. The combination of CNC, a nanomaterial scarcely exploited in immunosensing, with MWCNTs enables the preparation of a novel dual immunosensor for the determination of CD biomarkers in clinical samples, including faeces. The developed bioplatform exhibits a high reproducibility and selectivity allowing the simultaneous determination of IL-12 and IL-23 using a smaller sample volume and in a much shorter assay time than those reported for each target with commercial ELISA kits. The dual immunosensor was successfully applied to the analysis of raw serum and faeces spiked with IL-12 and IL-23 at the expected levels in patients with severe CD.
Graphical Abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>ISSN: 1436-5073</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-024-06868-2</identifier><identifier>PMID: 39630365</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Antibodies ; Antibodies, Immobilized - immunology ; Biomarkers ; Biomarkers - analysis ; Biomarkers - blood ; Biosensing Techniques - methods ; Cellulose - chemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Conjugation ; Crohn Disease - diagnosis ; Crohn's disease ; Diazonium Compounds - chemistry ; Electrical measurement ; Electrochemical Techniques - instrumentation ; Electrochemical Techniques - methods ; Electrodes ; Feces ; Feces - chemistry ; Humans ; Hydrogen peroxide ; Immunoassay - methods ; Immunosensors ; Interleukin-12 - blood ; Interleukin-12 - immunology ; Interleukin-23 - blood ; Interleukin-23 - immunology ; Limit of Detection ; Microengineering ; Multi wall carbon nanotubes ; Nanochemistry ; Nanomaterials ; Nanotechnology ; Nanotubes, Carbon - chemistry</subject><ispartof>Mikrochimica acta (1966), 2025-01, Vol.192 (1), p.4, Article 4</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024 Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><rights>Copyright Springer Nature B.V. 2025</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-9e4ca57b6930d50205b25f91df408857f184affcd91ee0f24c599625e0d5d95c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-024-06868-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-024-06868-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39630365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García-Rodrigo, Lorena</creatorcontrib><creatorcontrib>Ramos-López, Claudia</creatorcontrib><creatorcontrib>Sánchez-Tirado, Esther</creatorcontrib><creatorcontrib>Agüí, Lourdes</creatorcontrib><creatorcontrib>González-Cortés, Araceli</creatorcontrib><creatorcontrib>Yáñez-Sedeño, Paloma</creatorcontrib><creatorcontrib>Pingarrón, José M.</creatorcontrib><title>Electrochemical immunosensing of Crohn’s disease biomarkers using diazonium salt-grafted crystalline nanocellulose/carbon nanotube-modified electrodes</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>The first dual immunosensor is reported for the determination of IL-12 and IL-23, two relevant biomarkers of Crohn’s disease (CD). The strategy relies on the selective capture of the targets by the respective antibodies which were covalently immobilized onto SPCEs modified with crystalline nanocellulose (CNC) and multi-walled carbon nanotubes (MWCNTs) followed by conjugation with a detector antibody labelled with poly-HRP-Strept and amperometric transduction using the H
2
O
2
/HQ system. The combination of CNC, a nanomaterial scarcely exploited in immunosensing, with MWCNTs enables the preparation of a novel dual immunosensor for the determination of CD biomarkers in clinical samples, including faeces. The developed bioplatform exhibits a high reproducibility and selectivity allowing the simultaneous determination of IL-12 and IL-23 using a smaller sample volume and in a much shorter assay time than those reported for each target with commercial ELISA kits. The dual immunosensor was successfully applied to the analysis of raw serum and faeces spiked with IL-12 and IL-23 at the expected levels in patients with severe CD.
Graphical Abstract</description><subject>Analytical Chemistry</subject><subject>Antibodies</subject><subject>Antibodies, Immobilized - immunology</subject><subject>Biomarkers</subject><subject>Biomarkers - analysis</subject><subject>Biomarkers - blood</subject><subject>Biosensing Techniques - methods</subject><subject>Cellulose - chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Conjugation</subject><subject>Crohn Disease - diagnosis</subject><subject>Crohn's disease</subject><subject>Diazonium Compounds - chemistry</subject><subject>Electrical measurement</subject><subject>Electrochemical Techniques - instrumentation</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrodes</subject><subject>Feces</subject><subject>Feces - chemistry</subject><subject>Humans</subject><subject>Hydrogen peroxide</subject><subject>Immunoassay - methods</subject><subject>Immunosensors</subject><subject>Interleukin-12 - blood</subject><subject>Interleukin-12 - immunology</subject><subject>Interleukin-23 - blood</subject><subject>Interleukin-23 - immunology</subject><subject>Limit of Detection</subject><subject>Microengineering</subject><subject>Multi wall carbon nanotubes</subject><subject>Nanochemistry</subject><subject>Nanomaterials</subject><subject>Nanotechnology</subject><subject>Nanotubes, Carbon - chemistry</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhS1ERaeFF2CBIrHpJvT6N8kSjQpFqsSmrCPHvp66JHaxk0W74jHg9fokeCYFJBYsrCvZ3zk-uoeQ1xTeUYDmPAMoEDWwclSr2po9IxsquKolNPw52QAwVXPVsGNykvMtAG0UEy_IMe8UB67khvy4GNHMKZobnLzRY-WnaQkxY8g-7Kroqm2KN-Hx-89cWZ9RZ6wGHyedvmLK1XKgrNcPMfhlqrIe53qXtJvRVibd51mPow9YBR2iwXFcxuJ9bnQaYjhczsuA9RStd75IcE1jMb8kR06PGV89zVPy5cPF9fayvvr88dP2_VVtmFRz3aEwWjaD6jhYCQzkwKTrqHUC2lY2jrZCO2dsRxHBMWFk1ykmsdC2k4afkrPV9y7FbwvmuZ983ifVAeOSe04FFG8um4K-_Qe9jUsKJd2eoqAEbUSh2EqZFHNO6Pq75Mu-7nsK_b63fu2tL731h956VkRvnqyXYUL7R_K7qALwFcjlKeww_f37P7a_AD-HqA0</recordid><startdate>20250101</startdate><enddate>20250101</enddate><creator>García-Rodrigo, Lorena</creator><creator>Ramos-López, Claudia</creator><creator>Sánchez-Tirado, Esther</creator><creator>Agüí, Lourdes</creator><creator>González-Cortés, Araceli</creator><creator>Yáñez-Sedeño, Paloma</creator><creator>Pingarrón, José M.</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20250101</creationdate><title>Electrochemical immunosensing of Crohn’s disease biomarkers using diazonium salt-grafted crystalline nanocellulose/carbon nanotube-modified electrodes</title><author>García-Rodrigo, Lorena ; Ramos-López, Claudia ; Sánchez-Tirado, Esther ; Agüí, Lourdes ; González-Cortés, Araceli ; Yáñez-Sedeño, Paloma ; Pingarrón, José M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-9e4ca57b6930d50205b25f91df408857f184affcd91ee0f24c599625e0d5d95c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Analytical Chemistry</topic><topic>Antibodies</topic><topic>Antibodies, Immobilized - immunology</topic><topic>Biomarkers</topic><topic>Biomarkers - analysis</topic><topic>Biomarkers - blood</topic><topic>Biosensing Techniques - methods</topic><topic>Cellulose - chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Conjugation</topic><topic>Crohn Disease - diagnosis</topic><topic>Crohn's disease</topic><topic>Diazonium Compounds - chemistry</topic><topic>Electrical measurement</topic><topic>Electrochemical Techniques - instrumentation</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrodes</topic><topic>Feces</topic><topic>Feces - chemistry</topic><topic>Humans</topic><topic>Hydrogen peroxide</topic><topic>Immunoassay - methods</topic><topic>Immunosensors</topic><topic>Interleukin-12 - blood</topic><topic>Interleukin-12 - immunology</topic><topic>Interleukin-23 - blood</topic><topic>Interleukin-23 - immunology</topic><topic>Limit of Detection</topic><topic>Microengineering</topic><topic>Multi wall carbon nanotubes</topic><topic>Nanochemistry</topic><topic>Nanomaterials</topic><topic>Nanotechnology</topic><topic>Nanotubes, Carbon - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García-Rodrigo, Lorena</creatorcontrib><creatorcontrib>Ramos-López, Claudia</creatorcontrib><creatorcontrib>Sánchez-Tirado, Esther</creatorcontrib><creatorcontrib>Agüí, Lourdes</creatorcontrib><creatorcontrib>González-Cortés, Araceli</creatorcontrib><creatorcontrib>Yáñez-Sedeño, Paloma</creatorcontrib><creatorcontrib>Pingarrón, José M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García-Rodrigo, Lorena</au><au>Ramos-López, Claudia</au><au>Sánchez-Tirado, Esther</au><au>Agüí, Lourdes</au><au>González-Cortés, Araceli</au><au>Yáñez-Sedeño, Paloma</au><au>Pingarrón, José M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical immunosensing of Crohn’s disease biomarkers using diazonium salt-grafted crystalline nanocellulose/carbon nanotube-modified electrodes</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2025-01-01</date><risdate>2025</risdate><volume>192</volume><issue>1</issue><spage>4</spage><pages>4-</pages><artnum>4</artnum><issn>0026-3672</issn><issn>1436-5073</issn><eissn>1436-5073</eissn><abstract>The first dual immunosensor is reported for the determination of IL-12 and IL-23, two relevant biomarkers of Crohn’s disease (CD). The strategy relies on the selective capture of the targets by the respective antibodies which were covalently immobilized onto SPCEs modified with crystalline nanocellulose (CNC) and multi-walled carbon nanotubes (MWCNTs) followed by conjugation with a detector antibody labelled with poly-HRP-Strept and amperometric transduction using the H
2
O
2
/HQ system. The combination of CNC, a nanomaterial scarcely exploited in immunosensing, with MWCNTs enables the preparation of a novel dual immunosensor for the determination of CD biomarkers in clinical samples, including faeces. The developed bioplatform exhibits a high reproducibility and selectivity allowing the simultaneous determination of IL-12 and IL-23 using a smaller sample volume and in a much shorter assay time than those reported for each target with commercial ELISA kits. The dual immunosensor was successfully applied to the analysis of raw serum and faeces spiked with IL-12 and IL-23 at the expected levels in patients with severe CD.
Graphical Abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>39630365</pmid><doi>10.1007/s00604-024-06868-2</doi></addata></record> |
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| subjects | Analytical Chemistry Antibodies Antibodies, Immobilized - immunology Biomarkers Biomarkers - analysis Biomarkers - blood Biosensing Techniques - methods Cellulose - chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Conjugation Crohn Disease - diagnosis Crohn's disease Diazonium Compounds - chemistry Electrical measurement Electrochemical Techniques - instrumentation Electrochemical Techniques - methods Electrodes Feces Feces - chemistry Humans Hydrogen peroxide Immunoassay - methods Immunosensors Interleukin-12 - blood Interleukin-12 - immunology Interleukin-23 - blood Interleukin-23 - immunology Limit of Detection Microengineering Multi wall carbon nanotubes Nanochemistry Nanomaterials Nanotechnology Nanotubes, Carbon - chemistry |
| title | Electrochemical immunosensing of Crohn’s disease biomarkers using diazonium salt-grafted crystalline nanocellulose/carbon nanotube-modified electrodes |
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