Cell rupture of Tetradesmus obliquus using high-pressure homogenization at the pilot scale and recovery of pigments and lipids
[Display omitted] •T. obliquus BR003 contains 299 µg β-carotene/g and 14 g lipids/100 g dry matter.•High-pressure homogenization at the pilot scale (HPH) damaged T. obliquus cell walls.•At 90 L/h, HPH released pigments and lipids at a lower specific energy consumption.•HPH optimization promoted a hi...
Gespeichert in:
Veröffentlicht in: | Food research international 2024-11, Vol.196, p.115113, Article 115113 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | |
container_start_page | 115113 |
container_title | Food research international |
container_volume | 196 |
creator | Miranda Júnior, José Roberto da Silva, César Augusto Sodré de Moura Guimarães, Luciano Rocha, Dilson Novais Alhaji, Adamu Muhammad de Oliveira, Eduardo Basílio Martins, Marcio Arêdes dos Reis Coimbra, Jane Sélia |
description | [Display omitted]
•T. obliquus BR003 contains 299 µg β-carotene/g and 14 g lipids/100 g dry matter.•High-pressure homogenization at the pilot scale (HPH) damaged T. obliquus cell walls.•At 90 L/h, HPH released pigments and lipids at a lower specific energy consumption.•HPH optimization promoted a high recovery of pigments and lipids.•The pressure and number of passes of HPH affected the biocompound recovery rates.
Microalgae are promising sources of intracellular metabolites such as proteins, polysaccharides, pigments, and lipids. Thus, this study applied high-pressure homogenization (HPH) techniques on a pilot scale to disrupt the cells of Tetradesmus obliquus. The effects of pressure (P; 150, 250, and 350 bar), suspension concentration (Cs; 1.0, 1.5, and 2.0 % w/v), and number of cycles (Nc; 5, 15, and 25) were evaluated in HPH via a Box–Behnken experimental design. Response surface methodology was applied to optimize the recovery rate (dTr) of pigments and lipids. The specific energy consumption (SEC) and color change gradient (ΔE) of the biomass during HPH were also assessed. The optimal HPH conditions for pigment extraction with 1.5 % Cs (w/v) were as follows: P = 312 bar and Nc = 22 for chlorophyll-a (0.83 g/100 g; dTr = 69 %; SEC = 47.50 kJ/g dry matter); P = 345 bar and Nc = 24 for chlorophyll-b (0.63 g/100 g; dTr = 80 %; SEC = 57.30 kJ/g dry matter); P = 345 bar and Nc = 24 for total carotenoids (0.53 g/100 g; dTr = 79 %; SEC = 54.12 kJ/g dry matter); and P = 350 bar and Nc = 25 for β-carotene (299 µg/g; dTr = 58 %; SEC = 62.08 kJ/g dry matter). The optimal HPH conditions for lipid extraction were P = 350 bar and Nc = 23, with a lipid recovery rate of ≥28 %. Cell disruption during HPH caused a change in the color of the biomass (ΔE) due to the release of intracellular biocompounds. Increasing P and Nc led to higher SECs, ΔE gradients, and pigment and lipid contents. Thus, the levels of recovered pigments and lipids can be indicators of cell disruption in T. obliquus. |
doi_str_mv | 10.1016/j.foodres.2024.115113 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3134333157</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0963996924011839</els_id><sourcerecordid>3134333157</sourcerecordid><originalsourceid>FETCH-LOGICAL-c243t-b3f887366c722500ccf002c2288a24bc9c5f746808e902d14cc047c1de62ce6b3</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVJaTZpfkKLjrl4qw9btk8hLOkHBHpJz0IejXe12JYjyYHk0N9ebXaTa08a0DPz8j6EfOFszRlX3_br3nsbMK4FE-Wa84pz-YGseFPLouZldUZWrFWyaFvVnpOLGPeMMVXV7SdyLluVibpZkb8bHAYaljktAanv6QOmYCzGcYnUd4N7XPKwRDdt6c5td8WcI-OB3fnRb3FyLyY5P1GTaNohnd3gE41gBqRmsjQg-CcMz4fTs9uOOKX4-jG42dn4mXzszRDx6vRekj_f7x42P4v73z9-bW7vCxClTEUn-yb3UgpqISrGAHrGBAjRNEaUHbRQ9XWpGtZgy4TlJQAra-AWlQBUnbwk18e7c_CPC8akRxchVzcT-iVqyWUppeRVndHqiELwMQbs9RzcaMKz5kwf1Ou9PqnXB_X6qD7vfT1FLN2I9n3rzXUGbo4A5qJPDoOO4HACtC5bStp695-If0U6mek</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3134333157</pqid></control><display><type>article</type><title>Cell rupture of Tetradesmus obliquus using high-pressure homogenization at the pilot scale and recovery of pigments and lipids</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Miranda Júnior, José Roberto ; da Silva, César Augusto Sodré ; de Moura Guimarães, Luciano ; Rocha, Dilson Novais ; Alhaji, Adamu Muhammad ; de Oliveira, Eduardo Basílio ; Martins, Marcio Arêdes ; dos Reis Coimbra, Jane Sélia</creator><creatorcontrib>Miranda Júnior, José Roberto ; da Silva, César Augusto Sodré ; de Moura Guimarães, Luciano ; Rocha, Dilson Novais ; Alhaji, Adamu Muhammad ; de Oliveira, Eduardo Basílio ; Martins, Marcio Arêdes ; dos Reis Coimbra, Jane Sélia</creatorcontrib><description>[Display omitted]
•T. obliquus BR003 contains 299 µg β-carotene/g and 14 g lipids/100 g dry matter.•High-pressure homogenization at the pilot scale (HPH) damaged T. obliquus cell walls.•At 90 L/h, HPH released pigments and lipids at a lower specific energy consumption.•HPH optimization promoted a high recovery of pigments and lipids.•The pressure and number of passes of HPH affected the biocompound recovery rates.
Microalgae are promising sources of intracellular metabolites such as proteins, polysaccharides, pigments, and lipids. Thus, this study applied high-pressure homogenization (HPH) techniques on a pilot scale to disrupt the cells of Tetradesmus obliquus. The effects of pressure (P; 150, 250, and 350 bar), suspension concentration (Cs; 1.0, 1.5, and 2.0 % w/v), and number of cycles (Nc; 5, 15, and 25) were evaluated in HPH via a Box–Behnken experimental design. Response surface methodology was applied to optimize the recovery rate (dTr) of pigments and lipids. The specific energy consumption (SEC) and color change gradient (ΔE) of the biomass during HPH were also assessed. The optimal HPH conditions for pigment extraction with 1.5 % Cs (w/v) were as follows: P = 312 bar and Nc = 22 for chlorophyll-a (0.83 g/100 g; dTr = 69 %; SEC = 47.50 kJ/g dry matter); P = 345 bar and Nc = 24 for chlorophyll-b (0.63 g/100 g; dTr = 80 %; SEC = 57.30 kJ/g dry matter); P = 345 bar and Nc = 24 for total carotenoids (0.53 g/100 g; dTr = 79 %; SEC = 54.12 kJ/g dry matter); and P = 350 bar and Nc = 25 for β-carotene (299 µg/g; dTr = 58 %; SEC = 62.08 kJ/g dry matter). The optimal HPH conditions for lipid extraction were P = 350 bar and Nc = 23, with a lipid recovery rate of ≥28 %. Cell disruption during HPH caused a change in the color of the biomass (ΔE) due to the release of intracellular biocompounds. Increasing P and Nc led to higher SECs, ΔE gradients, and pigment and lipid contents. Thus, the levels of recovered pigments and lipids can be indicators of cell disruption in T. obliquus.</description><identifier>ISSN: 0963-9969</identifier><identifier>ISSN: 1873-7145</identifier><identifier>EISSN: 1873-7145</identifier><identifier>DOI: 10.1016/j.foodres.2024.115113</identifier><identifier>PMID: 39614578</identifier><language>eng</language><publisher>Canada: Elsevier Ltd</publisher><subject>Biocompounds ; Biomass ; Cell disruption ; Chlorophyll - analysis ; Chlorophyll A - analysis ; Color change ; Energy consumption ; Extraction ; Lipids - analysis ; Microalgae - chemistry ; Optimization ; Pigments, Biological - analysis ; Pilot Projects ; Pressure</subject><ispartof>Food research international, 2024-11, Vol.196, p.115113, Article 115113</ispartof><rights>2024 Elsevier Ltd</rights><rights>Copyright © 2024 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c243t-b3f887366c722500ccf002c2288a24bc9c5f746808e902d14cc047c1de62ce6b3</cites><orcidid>0000-0002-5998-189X ; 0000-0002-8413-211X ; 0000-0002-7302-7058 ; 0009-0003-0255-3602 ; 0000-0001-9656-2996 ; 0000-0001-9550-3142 ; 0000-0001-5705-9431 ; 0000-0002-2329-2507</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodres.2024.115113$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39614578$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miranda Júnior, José Roberto</creatorcontrib><creatorcontrib>da Silva, César Augusto Sodré</creatorcontrib><creatorcontrib>de Moura Guimarães, Luciano</creatorcontrib><creatorcontrib>Rocha, Dilson Novais</creatorcontrib><creatorcontrib>Alhaji, Adamu Muhammad</creatorcontrib><creatorcontrib>de Oliveira, Eduardo Basílio</creatorcontrib><creatorcontrib>Martins, Marcio Arêdes</creatorcontrib><creatorcontrib>dos Reis Coimbra, Jane Sélia</creatorcontrib><title>Cell rupture of Tetradesmus obliquus using high-pressure homogenization at the pilot scale and recovery of pigments and lipids</title><title>Food research international</title><addtitle>Food Res Int</addtitle><description>[Display omitted]
•T. obliquus BR003 contains 299 µg β-carotene/g and 14 g lipids/100 g dry matter.•High-pressure homogenization at the pilot scale (HPH) damaged T. obliquus cell walls.•At 90 L/h, HPH released pigments and lipids at a lower specific energy consumption.•HPH optimization promoted a high recovery of pigments and lipids.•The pressure and number of passes of HPH affected the biocompound recovery rates.
Microalgae are promising sources of intracellular metabolites such as proteins, polysaccharides, pigments, and lipids. Thus, this study applied high-pressure homogenization (HPH) techniques on a pilot scale to disrupt the cells of Tetradesmus obliquus. The effects of pressure (P; 150, 250, and 350 bar), suspension concentration (Cs; 1.0, 1.5, and 2.0 % w/v), and number of cycles (Nc; 5, 15, and 25) were evaluated in HPH via a Box–Behnken experimental design. Response surface methodology was applied to optimize the recovery rate (dTr) of pigments and lipids. The specific energy consumption (SEC) and color change gradient (ΔE) of the biomass during HPH were also assessed. The optimal HPH conditions for pigment extraction with 1.5 % Cs (w/v) were as follows: P = 312 bar and Nc = 22 for chlorophyll-a (0.83 g/100 g; dTr = 69 %; SEC = 47.50 kJ/g dry matter); P = 345 bar and Nc = 24 for chlorophyll-b (0.63 g/100 g; dTr = 80 %; SEC = 57.30 kJ/g dry matter); P = 345 bar and Nc = 24 for total carotenoids (0.53 g/100 g; dTr = 79 %; SEC = 54.12 kJ/g dry matter); and P = 350 bar and Nc = 25 for β-carotene (299 µg/g; dTr = 58 %; SEC = 62.08 kJ/g dry matter). The optimal HPH conditions for lipid extraction were P = 350 bar and Nc = 23, with a lipid recovery rate of ≥28 %. Cell disruption during HPH caused a change in the color of the biomass (ΔE) due to the release of intracellular biocompounds. Increasing P and Nc led to higher SECs, ΔE gradients, and pigment and lipid contents. Thus, the levels of recovered pigments and lipids can be indicators of cell disruption in T. obliquus.</description><subject>Biocompounds</subject><subject>Biomass</subject><subject>Cell disruption</subject><subject>Chlorophyll - analysis</subject><subject>Chlorophyll A - analysis</subject><subject>Color change</subject><subject>Energy consumption</subject><subject>Extraction</subject><subject>Lipids - analysis</subject><subject>Microalgae - chemistry</subject><subject>Optimization</subject><subject>Pigments, Biological - analysis</subject><subject>Pilot Projects</subject><subject>Pressure</subject><issn>0963-9969</issn><issn>1873-7145</issn><issn>1873-7145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVJaTZpfkKLjrl4qw9btk8hLOkHBHpJz0IejXe12JYjyYHk0N9ebXaTa08a0DPz8j6EfOFszRlX3_br3nsbMK4FE-Wa84pz-YGseFPLouZldUZWrFWyaFvVnpOLGPeMMVXV7SdyLluVibpZkb8bHAYaljktAanv6QOmYCzGcYnUd4N7XPKwRDdt6c5td8WcI-OB3fnRb3FyLyY5P1GTaNohnd3gE41gBqRmsjQg-CcMz4fTs9uOOKX4-jG42dn4mXzszRDx6vRekj_f7x42P4v73z9-bW7vCxClTEUn-yb3UgpqISrGAHrGBAjRNEaUHbRQ9XWpGtZgy4TlJQAra-AWlQBUnbwk18e7c_CPC8akRxchVzcT-iVqyWUppeRVndHqiELwMQbs9RzcaMKz5kwf1Ou9PqnXB_X6qD7vfT1FLN2I9n3rzXUGbo4A5qJPDoOO4HACtC5bStp695-If0U6mek</recordid><startdate>202411</startdate><enddate>202411</enddate><creator>Miranda Júnior, José Roberto</creator><creator>da Silva, César Augusto Sodré</creator><creator>de Moura Guimarães, Luciano</creator><creator>Rocha, Dilson Novais</creator><creator>Alhaji, Adamu Muhammad</creator><creator>de Oliveira, Eduardo Basílio</creator><creator>Martins, Marcio Arêdes</creator><creator>dos Reis Coimbra, Jane Sélia</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5998-189X</orcidid><orcidid>https://orcid.org/0000-0002-8413-211X</orcidid><orcidid>https://orcid.org/0000-0002-7302-7058</orcidid><orcidid>https://orcid.org/0009-0003-0255-3602</orcidid><orcidid>https://orcid.org/0000-0001-9656-2996</orcidid><orcidid>https://orcid.org/0000-0001-9550-3142</orcidid><orcidid>https://orcid.org/0000-0001-5705-9431</orcidid><orcidid>https://orcid.org/0000-0002-2329-2507</orcidid></search><sort><creationdate>202411</creationdate><title>Cell rupture of Tetradesmus obliquus using high-pressure homogenization at the pilot scale and recovery of pigments and lipids</title><author>Miranda Júnior, José Roberto ; da Silva, César Augusto Sodré ; de Moura Guimarães, Luciano ; Rocha, Dilson Novais ; Alhaji, Adamu Muhammad ; de Oliveira, Eduardo Basílio ; Martins, Marcio Arêdes ; dos Reis Coimbra, Jane Sélia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c243t-b3f887366c722500ccf002c2288a24bc9c5f746808e902d14cc047c1de62ce6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biocompounds</topic><topic>Biomass</topic><topic>Cell disruption</topic><topic>Chlorophyll - analysis</topic><topic>Chlorophyll A - analysis</topic><topic>Color change</topic><topic>Energy consumption</topic><topic>Extraction</topic><topic>Lipids - analysis</topic><topic>Microalgae - chemistry</topic><topic>Optimization</topic><topic>Pigments, Biological - analysis</topic><topic>Pilot Projects</topic><topic>Pressure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miranda Júnior, José Roberto</creatorcontrib><creatorcontrib>da Silva, César Augusto Sodré</creatorcontrib><creatorcontrib>de Moura Guimarães, Luciano</creatorcontrib><creatorcontrib>Rocha, Dilson Novais</creatorcontrib><creatorcontrib>Alhaji, Adamu Muhammad</creatorcontrib><creatorcontrib>de Oliveira, Eduardo Basílio</creatorcontrib><creatorcontrib>Martins, Marcio Arêdes</creatorcontrib><creatorcontrib>dos Reis Coimbra, Jane Sélia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miranda Júnior, José Roberto</au><au>da Silva, César Augusto Sodré</au><au>de Moura Guimarães, Luciano</au><au>Rocha, Dilson Novais</au><au>Alhaji, Adamu Muhammad</au><au>de Oliveira, Eduardo Basílio</au><au>Martins, Marcio Arêdes</au><au>dos Reis Coimbra, Jane Sélia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell rupture of Tetradesmus obliquus using high-pressure homogenization at the pilot scale and recovery of pigments and lipids</atitle><jtitle>Food research international</jtitle><addtitle>Food Res Int</addtitle><date>2024-11</date><risdate>2024</risdate><volume>196</volume><spage>115113</spage><pages>115113-</pages><artnum>115113</artnum><issn>0963-9969</issn><issn>1873-7145</issn><eissn>1873-7145</eissn><abstract>[Display omitted]
•T. obliquus BR003 contains 299 µg β-carotene/g and 14 g lipids/100 g dry matter.•High-pressure homogenization at the pilot scale (HPH) damaged T. obliquus cell walls.•At 90 L/h, HPH released pigments and lipids at a lower specific energy consumption.•HPH optimization promoted a high recovery of pigments and lipids.•The pressure and number of passes of HPH affected the biocompound recovery rates.
Microalgae are promising sources of intracellular metabolites such as proteins, polysaccharides, pigments, and lipids. Thus, this study applied high-pressure homogenization (HPH) techniques on a pilot scale to disrupt the cells of Tetradesmus obliquus. The effects of pressure (P; 150, 250, and 350 bar), suspension concentration (Cs; 1.0, 1.5, and 2.0 % w/v), and number of cycles (Nc; 5, 15, and 25) were evaluated in HPH via a Box–Behnken experimental design. Response surface methodology was applied to optimize the recovery rate (dTr) of pigments and lipids. The specific energy consumption (SEC) and color change gradient (ΔE) of the biomass during HPH were also assessed. The optimal HPH conditions for pigment extraction with 1.5 % Cs (w/v) were as follows: P = 312 bar and Nc = 22 for chlorophyll-a (0.83 g/100 g; dTr = 69 %; SEC = 47.50 kJ/g dry matter); P = 345 bar and Nc = 24 for chlorophyll-b (0.63 g/100 g; dTr = 80 %; SEC = 57.30 kJ/g dry matter); P = 345 bar and Nc = 24 for total carotenoids (0.53 g/100 g; dTr = 79 %; SEC = 54.12 kJ/g dry matter); and P = 350 bar and Nc = 25 for β-carotene (299 µg/g; dTr = 58 %; SEC = 62.08 kJ/g dry matter). The optimal HPH conditions for lipid extraction were P = 350 bar and Nc = 23, with a lipid recovery rate of ≥28 %. Cell disruption during HPH caused a change in the color of the biomass (ΔE) due to the release of intracellular biocompounds. Increasing P and Nc led to higher SECs, ΔE gradients, and pigment and lipid contents. Thus, the levels of recovered pigments and lipids can be indicators of cell disruption in T. obliquus.</abstract><cop>Canada</cop><pub>Elsevier Ltd</pub><pmid>39614578</pmid><doi>10.1016/j.foodres.2024.115113</doi><orcidid>https://orcid.org/0000-0002-5998-189X</orcidid><orcidid>https://orcid.org/0000-0002-8413-211X</orcidid><orcidid>https://orcid.org/0000-0002-7302-7058</orcidid><orcidid>https://orcid.org/0009-0003-0255-3602</orcidid><orcidid>https://orcid.org/0000-0001-9656-2996</orcidid><orcidid>https://orcid.org/0000-0001-9550-3142</orcidid><orcidid>https://orcid.org/0000-0001-5705-9431</orcidid><orcidid>https://orcid.org/0000-0002-2329-2507</orcidid></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0963-9969 |
ispartof | Food research international, 2024-11, Vol.196, p.115113, Article 115113 |
issn | 0963-9969 1873-7145 1873-7145 |
language | eng |
recordid | cdi_proquest_miscellaneous_3134333157 |
source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Biocompounds Biomass Cell disruption Chlorophyll - analysis Chlorophyll A - analysis Color change Energy consumption Extraction Lipids - analysis Microalgae - chemistry Optimization Pigments, Biological - analysis Pilot Projects Pressure |
title | Cell rupture of Tetradesmus obliquus using high-pressure homogenization at the pilot scale and recovery of pigments and lipids |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T16%3A23%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cell%20rupture%20of%20Tetradesmus%20obliquus%20using%20high-pressure%20homogenization%20at%20the%20pilot%20scale%20and%20recovery%20of%20pigments%20and%20lipids&rft.jtitle=Food%20research%20international&rft.au=Miranda%20J%C3%BAnior,%20Jos%C3%A9%20Roberto&rft.date=2024-11&rft.volume=196&rft.spage=115113&rft.pages=115113-&rft.artnum=115113&rft.issn=0963-9969&rft.eissn=1873-7145&rft_id=info:doi/10.1016/j.foodres.2024.115113&rft_dat=%3Cproquest_cross%3E3134333157%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3134333157&rft_id=info:pmid/39614578&rft_els_id=S0963996924011839&rfr_iscdi=true |