Cell rupture of Tetradesmus obliquus using high-pressure homogenization at the pilot scale and recovery of pigments and lipids

[Display omitted] •T. obliquus BR003 contains 299 µg β-carotene/g and 14 g lipids/100 g dry matter.•High-pressure homogenization at the pilot scale (HPH) damaged T. obliquus cell walls.•At 90 L/h, HPH released pigments and lipids at a lower specific energy consumption.•HPH optimization promoted a hi...

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Veröffentlicht in:Food research international 2024-11, Vol.196, p.115113, Article 115113
Hauptverfasser: Miranda Júnior, José Roberto, da Silva, César Augusto Sodré, de Moura Guimarães, Luciano, Rocha, Dilson Novais, Alhaji, Adamu Muhammad, de Oliveira, Eduardo Basílio, Martins, Marcio Arêdes, dos Reis Coimbra, Jane Sélia
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container_title Food research international
container_volume 196
creator Miranda Júnior, José Roberto
da Silva, César Augusto Sodré
de Moura Guimarães, Luciano
Rocha, Dilson Novais
Alhaji, Adamu Muhammad
de Oliveira, Eduardo Basílio
Martins, Marcio Arêdes
dos Reis Coimbra, Jane Sélia
description [Display omitted] •T. obliquus BR003 contains 299 µg β-carotene/g and 14 g lipids/100 g dry matter.•High-pressure homogenization at the pilot scale (HPH) damaged T. obliquus cell walls.•At 90 L/h, HPH released pigments and lipids at a lower specific energy consumption.•HPH optimization promoted a high recovery of pigments and lipids.•The pressure and number of passes of HPH affected the biocompound recovery rates. Microalgae are promising sources of intracellular metabolites such as proteins, polysaccharides, pigments, and lipids. Thus, this study applied high-pressure homogenization (HPH) techniques on a pilot scale to disrupt the cells of Tetradesmus obliquus. The effects of pressure (P; 150, 250, and 350 bar), suspension concentration (Cs; 1.0, 1.5, and 2.0 % w/v), and number of cycles (Nc; 5, 15, and 25) were evaluated in HPH via a Box–Behnken experimental design. Response surface methodology was applied to optimize the recovery rate (dTr) of pigments and lipids. The specific energy consumption (SEC) and color change gradient (ΔE) of the biomass during HPH were also assessed. The optimal HPH conditions for pigment extraction with 1.5 % Cs (w/v) were as follows: P = 312 bar and Nc = 22 for chlorophyll-a (0.83 g/100 g; dTr = 69 %; SEC = 47.50 kJ/g dry matter); P = 345 bar and Nc = 24 for chlorophyll-b (0.63 g/100 g; dTr = 80 %; SEC = 57.30 kJ/g dry matter); P = 345 bar and Nc = 24 for total carotenoids (0.53 g/100 g; dTr = 79 %; SEC = 54.12 kJ/g dry matter); and P = 350 bar and Nc = 25 for β-carotene (299 µg/g; dTr = 58 %; SEC = 62.08 kJ/g dry matter). The optimal HPH conditions for lipid extraction were P = 350 bar and Nc = 23, with a lipid recovery rate of ≥28 %. Cell disruption during HPH caused a change in the color of the biomass (ΔE) due to the release of intracellular biocompounds. Increasing P and Nc led to higher SECs, ΔE gradients, and pigment and lipid contents. Thus, the levels of recovered pigments and lipids can be indicators of cell disruption in T. obliquus.
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Microalgae are promising sources of intracellular metabolites such as proteins, polysaccharides, pigments, and lipids. Thus, this study applied high-pressure homogenization (HPH) techniques on a pilot scale to disrupt the cells of Tetradesmus obliquus. The effects of pressure (P; 150, 250, and 350 bar), suspension concentration (Cs; 1.0, 1.5, and 2.0 % w/v), and number of cycles (Nc; 5, 15, and 25) were evaluated in HPH via a Box–Behnken experimental design. Response surface methodology was applied to optimize the recovery rate (dTr) of pigments and lipids. The specific energy consumption (SEC) and color change gradient (ΔE) of the biomass during HPH were also assessed. The optimal HPH conditions for pigment extraction with 1.5 % Cs (w/v) were as follows: P = 312 bar and Nc = 22 for chlorophyll-a (0.83 g/100 g; dTr = 69 %; SEC = 47.50 kJ/g dry matter); P = 345 bar and Nc = 24 for chlorophyll-b (0.63 g/100 g; dTr = 80 %; SEC = 57.30 kJ/g dry matter); P = 345 bar and Nc = 24 for total carotenoids (0.53 g/100 g; dTr = 79 %; SEC = 54.12 kJ/g dry matter); and P = 350 bar and Nc = 25 for β-carotene (299 µg/g; dTr = 58 %; SEC = 62.08 kJ/g dry matter). The optimal HPH conditions for lipid extraction were P = 350 bar and Nc = 23, with a lipid recovery rate of ≥28 %. Cell disruption during HPH caused a change in the color of the biomass (ΔE) due to the release of intracellular biocompounds. Increasing P and Nc led to higher SECs, ΔE gradients, and pigment and lipid contents. 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Microalgae are promising sources of intracellular metabolites such as proteins, polysaccharides, pigments, and lipids. Thus, this study applied high-pressure homogenization (HPH) techniques on a pilot scale to disrupt the cells of Tetradesmus obliquus. The effects of pressure (P; 150, 250, and 350 bar), suspension concentration (Cs; 1.0, 1.5, and 2.0 % w/v), and number of cycles (Nc; 5, 15, and 25) were evaluated in HPH via a Box–Behnken experimental design. Response surface methodology was applied to optimize the recovery rate (dTr) of pigments and lipids. The specific energy consumption (SEC) and color change gradient (ΔE) of the biomass during HPH were also assessed. The optimal HPH conditions for pigment extraction with 1.5 % Cs (w/v) were as follows: P = 312 bar and Nc = 22 for chlorophyll-a (0.83 g/100 g; dTr = 69 %; SEC = 47.50 kJ/g dry matter); P = 345 bar and Nc = 24 for chlorophyll-b (0.63 g/100 g; dTr = 80 %; SEC = 57.30 kJ/g dry matter); P = 345 bar and Nc = 24 for total carotenoids (0.53 g/100 g; dTr = 79 %; SEC = 54.12 kJ/g dry matter); and P = 350 bar and Nc = 25 for β-carotene (299 µg/g; dTr = 58 %; SEC = 62.08 kJ/g dry matter). The optimal HPH conditions for lipid extraction were P = 350 bar and Nc = 23, with a lipid recovery rate of ≥28 %. Cell disruption during HPH caused a change in the color of the biomass (ΔE) due to the release of intracellular biocompounds. Increasing P and Nc led to higher SECs, ΔE gradients, and pigment and lipid contents. 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Microalgae are promising sources of intracellular metabolites such as proteins, polysaccharides, pigments, and lipids. Thus, this study applied high-pressure homogenization (HPH) techniques on a pilot scale to disrupt the cells of Tetradesmus obliquus. The effects of pressure (P; 150, 250, and 350 bar), suspension concentration (Cs; 1.0, 1.5, and 2.0 % w/v), and number of cycles (Nc; 5, 15, and 25) were evaluated in HPH via a Box–Behnken experimental design. Response surface methodology was applied to optimize the recovery rate (dTr) of pigments and lipids. The specific energy consumption (SEC) and color change gradient (ΔE) of the biomass during HPH were also assessed. The optimal HPH conditions for pigment extraction with 1.5 % Cs (w/v) were as follows: P = 312 bar and Nc = 22 for chlorophyll-a (0.83 g/100 g; dTr = 69 %; SEC = 47.50 kJ/g dry matter); P = 345 bar and Nc = 24 for chlorophyll-b (0.63 g/100 g; dTr = 80 %; SEC = 57.30 kJ/g dry matter); P = 345 bar and Nc = 24 for total carotenoids (0.53 g/100 g; dTr = 79 %; SEC = 54.12 kJ/g dry matter); and P = 350 bar and Nc = 25 for β-carotene (299 µg/g; dTr = 58 %; SEC = 62.08 kJ/g dry matter). The optimal HPH conditions for lipid extraction were P = 350 bar and Nc = 23, with a lipid recovery rate of ≥28 %. Cell disruption during HPH caused a change in the color of the biomass (ΔE) due to the release of intracellular biocompounds. Increasing P and Nc led to higher SECs, ΔE gradients, and pigment and lipid contents. 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subjects Biocompounds
Biomass
Cell disruption
Chlorophyll - analysis
Chlorophyll A - analysis
Color change
Energy consumption
Extraction
Lipids - analysis
Microalgae - chemistry
Optimization
Pigments, Biological - analysis
Pilot Projects
Pressure
title Cell rupture of Tetradesmus obliquus using high-pressure homogenization at the pilot scale and recovery of pigments and lipids
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