OsPUKI, a PfkB protein, regulates seed germination in rice by influencing ABA synthesis
•OsPUKI, encodes a pseudouridine kinase, is highly expressed at stage of seed germination.•Mutation in OsPUKI leads to delayed seed germination and shorter shoot length.•OsPUKI may affect rice seed germination by influencing ABA synthesis. Rice seed germination is a crucial phase in rice growth and...
Gespeichert in:
| Veröffentlicht in: | Gene 2025-02, Vol.936, p.149118, Article 149118 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 149118 |
| container_title | Gene |
| container_volume | 936 |
| creator | Bao, Yi Zhang, Zongfei Peng, Ni Qiu, Ziting Yan, Xin Ouyang, Jiexiu Li, Shaobo Wang, Xin |
| description | •OsPUKI, encodes a pseudouridine kinase, is highly expressed at stage of seed germination.•Mutation in OsPUKI leads to delayed seed germination and shorter shoot length.•OsPUKI may affect rice seed germination by influencing ABA synthesis.
Rice seed germination is a crucial phase in rice growth and development, but its molecular mechanism has not been fully elucidated. In this study, we investigated the function of rice pfkB family gene OsPUKI in seed germination. Compared with WT (ZH11), ospuki mutants showed delayed seed germination and shorter shoot length. QRT-PCR results showed that OsPUKI was highly expressed in leaves and developing seeds of 21-day after pollination, and was highly expressed at the early stage of seed germination. GC-MS analysis demonstrated that content of abscisic acid (ABA) in ospuki-3 was higher than that in WT. QRT-PCR analysis revealed that ospuki mutants had higher transcription levels of ABA synthesis-related genes OsNCED2, OsNCED3, OsNCED4, and OsZEP1. Furthermore, it was shown that ospuki mutants were more sensitive to fluridone (Flu) during seed germination and seedling growth than WT according to exogenous Flu treatment experiments. In short, our findings suggest that OsPUKI may positively regulates rice seed germination by influencing ABA synthesis. |
| doi_str_mv | 10.1016/j.gene.2024.149118 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3132366105</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378111924009995</els_id><sourcerecordid>3132366105</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1526-674e49e3abd75d675b1ed6ac5842f6aac0a8e5ded70ffc90b085c71458adb2863</originalsourceid><addsrcrecordid>eNp9kMFO4zAQhi0EWgq7L8AB-ciBFI8TO47EpUUsIJDaA2iPlmNPikvqgJ2s1LffVAWOO5eZwze_Zj5CzoBNgYG8Wk9XGHDKGS-mUFQA6oBMQJVVxliuDsmE5aXKAKA6JicprdlYQvAf5DivhGLA5YT8WaTly-PDJTV02bzN6XvsevThkkZcDa3pMdGE6OgK48YH0_suUB9o9BZpvR3Hph0wWB9WdDaf0bQN_Ssmn36So8a0CX999lPy8vv2-eY-e1rcPdzMnjILgstMlgUWFeamdqVwshQ1oJPGClXwRhpjmVEoHLqSNY2tWM2UsCUUQhlXcyXzU3Kxzx0P_xgw9Xrjk8W2NQG7Iekccp5LCUyMKN-jNnYpRWz0e_QbE7camN4J1Wu9E6p3QvVe6Lh0_pk_1Bt03ytfBkfgeg_g-OVfj1En60cj6HxE22vX-f_l_wM4r4YS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3132366105</pqid></control><display><type>article</type><title>OsPUKI, a PfkB protein, regulates seed germination in rice by influencing ABA synthesis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Bao, Yi ; Zhang, Zongfei ; Peng, Ni ; Qiu, Ziting ; Yan, Xin ; Ouyang, Jiexiu ; Li, Shaobo ; Wang, Xin</creator><creatorcontrib>Bao, Yi ; Zhang, Zongfei ; Peng, Ni ; Qiu, Ziting ; Yan, Xin ; Ouyang, Jiexiu ; Li, Shaobo ; Wang, Xin</creatorcontrib><description>•OsPUKI, encodes a pseudouridine kinase, is highly expressed at stage of seed germination.•Mutation in OsPUKI leads to delayed seed germination and shorter shoot length.•OsPUKI may affect rice seed germination by influencing ABA synthesis.
Rice seed germination is a crucial phase in rice growth and development, but its molecular mechanism has not been fully elucidated. In this study, we investigated the function of rice pfkB family gene OsPUKI in seed germination. Compared with WT (ZH11), ospuki mutants showed delayed seed germination and shorter shoot length. QRT-PCR results showed that OsPUKI was highly expressed in leaves and developing seeds of 21-day after pollination, and was highly expressed at the early stage of seed germination. GC-MS analysis demonstrated that content of abscisic acid (ABA) in ospuki-3 was higher than that in WT. QRT-PCR analysis revealed that ospuki mutants had higher transcription levels of ABA synthesis-related genes OsNCED2, OsNCED3, OsNCED4, and OsZEP1. Furthermore, it was shown that ospuki mutants were more sensitive to fluridone (Flu) during seed germination and seedling growth than WT according to exogenous Flu treatment experiments. In short, our findings suggest that OsPUKI may positively regulates rice seed germination by influencing ABA synthesis.</description><identifier>ISSN: 0378-1119</identifier><identifier>ISSN: 1879-0038</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2024.149118</identifier><identifier>PMID: 39580126</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>ABA ; Abscisic Acid - metabolism ; Gene Expression Regulation, Plant ; Germination - genetics ; Mutation ; Oryza - genetics ; Oryza - growth & development ; Oryza - metabolism ; Oryza sativa ; OsPUKI ; pfkB ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Pyridones ; Seed germination ; Seedlings - genetics ; Seedlings - growth & development ; Seeds - genetics ; Seeds - growth & development</subject><ispartof>Gene, 2025-02, Vol.936, p.149118, Article 149118</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1526-674e49e3abd75d675b1ed6ac5842f6aac0a8e5ded70ffc90b085c71458adb2863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gene.2024.149118$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39580126$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bao, Yi</creatorcontrib><creatorcontrib>Zhang, Zongfei</creatorcontrib><creatorcontrib>Peng, Ni</creatorcontrib><creatorcontrib>Qiu, Ziting</creatorcontrib><creatorcontrib>Yan, Xin</creatorcontrib><creatorcontrib>Ouyang, Jiexiu</creatorcontrib><creatorcontrib>Li, Shaobo</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><title>OsPUKI, a PfkB protein, regulates seed germination in rice by influencing ABA synthesis</title><title>Gene</title><addtitle>Gene</addtitle><description>•OsPUKI, encodes a pseudouridine kinase, is highly expressed at stage of seed germination.•Mutation in OsPUKI leads to delayed seed germination and shorter shoot length.•OsPUKI may affect rice seed germination by influencing ABA synthesis.
Rice seed germination is a crucial phase in rice growth and development, but its molecular mechanism has not been fully elucidated. In this study, we investigated the function of rice pfkB family gene OsPUKI in seed germination. Compared with WT (ZH11), ospuki mutants showed delayed seed germination and shorter shoot length. QRT-PCR results showed that OsPUKI was highly expressed in leaves and developing seeds of 21-day after pollination, and was highly expressed at the early stage of seed germination. GC-MS analysis demonstrated that content of abscisic acid (ABA) in ospuki-3 was higher than that in WT. QRT-PCR analysis revealed that ospuki mutants had higher transcription levels of ABA synthesis-related genes OsNCED2, OsNCED3, OsNCED4, and OsZEP1. Furthermore, it was shown that ospuki mutants were more sensitive to fluridone (Flu) during seed germination and seedling growth than WT according to exogenous Flu treatment experiments. In short, our findings suggest that OsPUKI may positively regulates rice seed germination by influencing ABA synthesis.</description><subject>ABA</subject><subject>Abscisic Acid - metabolism</subject><subject>Gene Expression Regulation, Plant</subject><subject>Germination - genetics</subject><subject>Mutation</subject><subject>Oryza - genetics</subject><subject>Oryza - growth & development</subject><subject>Oryza - metabolism</subject><subject>Oryza sativa</subject><subject>OsPUKI</subject><subject>pfkB</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Pyridones</subject><subject>Seed germination</subject><subject>Seedlings - genetics</subject><subject>Seedlings - growth & development</subject><subject>Seeds - genetics</subject><subject>Seeds - growth & development</subject><issn>0378-1119</issn><issn>1879-0038</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFO4zAQhi0EWgq7L8AB-ciBFI8TO47EpUUsIJDaA2iPlmNPikvqgJ2s1LffVAWOO5eZwze_Zj5CzoBNgYG8Wk9XGHDKGS-mUFQA6oBMQJVVxliuDsmE5aXKAKA6JicprdlYQvAf5DivhGLA5YT8WaTly-PDJTV02bzN6XvsevThkkZcDa3pMdGE6OgK48YH0_suUB9o9BZpvR3Hph0wWB9WdDaf0bQN_Ssmn36So8a0CX999lPy8vv2-eY-e1rcPdzMnjILgstMlgUWFeamdqVwshQ1oJPGClXwRhpjmVEoHLqSNY2tWM2UsCUUQhlXcyXzU3Kxzx0P_xgw9Xrjk8W2NQG7Iekccp5LCUyMKN-jNnYpRWz0e_QbE7camN4J1Wu9E6p3QvVe6Lh0_pk_1Bt03ytfBkfgeg_g-OVfj1En60cj6HxE22vX-f_l_wM4r4YS</recordid><startdate>20250205</startdate><enddate>20250205</enddate><creator>Bao, Yi</creator><creator>Zhang, Zongfei</creator><creator>Peng, Ni</creator><creator>Qiu, Ziting</creator><creator>Yan, Xin</creator><creator>Ouyang, Jiexiu</creator><creator>Li, Shaobo</creator><creator>Wang, Xin</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20250205</creationdate><title>OsPUKI, a PfkB protein, regulates seed germination in rice by influencing ABA synthesis</title><author>Bao, Yi ; Zhang, Zongfei ; Peng, Ni ; Qiu, Ziting ; Yan, Xin ; Ouyang, Jiexiu ; Li, Shaobo ; Wang, Xin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1526-674e49e3abd75d675b1ed6ac5842f6aac0a8e5ded70ffc90b085c71458adb2863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>ABA</topic><topic>Abscisic Acid - metabolism</topic><topic>Gene Expression Regulation, Plant</topic><topic>Germination - genetics</topic><topic>Mutation</topic><topic>Oryza - genetics</topic><topic>Oryza - growth & development</topic><topic>Oryza - metabolism</topic><topic>Oryza sativa</topic><topic>OsPUKI</topic><topic>pfkB</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Pyridones</topic><topic>Seed germination</topic><topic>Seedlings - genetics</topic><topic>Seedlings - growth & development</topic><topic>Seeds - genetics</topic><topic>Seeds - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bao, Yi</creatorcontrib><creatorcontrib>Zhang, Zongfei</creatorcontrib><creatorcontrib>Peng, Ni</creatorcontrib><creatorcontrib>Qiu, Ziting</creatorcontrib><creatorcontrib>Yan, Xin</creatorcontrib><creatorcontrib>Ouyang, Jiexiu</creatorcontrib><creatorcontrib>Li, Shaobo</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bao, Yi</au><au>Zhang, Zongfei</au><au>Peng, Ni</au><au>Qiu, Ziting</au><au>Yan, Xin</au><au>Ouyang, Jiexiu</au><au>Li, Shaobo</au><au>Wang, Xin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>OsPUKI, a PfkB protein, regulates seed germination in rice by influencing ABA synthesis</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2025-02-05</date><risdate>2025</risdate><volume>936</volume><spage>149118</spage><pages>149118-</pages><artnum>149118</artnum><issn>0378-1119</issn><issn>1879-0038</issn><eissn>1879-0038</eissn><abstract>•OsPUKI, encodes a pseudouridine kinase, is highly expressed at stage of seed germination.•Mutation in OsPUKI leads to delayed seed germination and shorter shoot length.•OsPUKI may affect rice seed germination by influencing ABA synthesis.
Rice seed germination is a crucial phase in rice growth and development, but its molecular mechanism has not been fully elucidated. In this study, we investigated the function of rice pfkB family gene OsPUKI in seed germination. Compared with WT (ZH11), ospuki mutants showed delayed seed germination and shorter shoot length. QRT-PCR results showed that OsPUKI was highly expressed in leaves and developing seeds of 21-day after pollination, and was highly expressed at the early stage of seed germination. GC-MS analysis demonstrated that content of abscisic acid (ABA) in ospuki-3 was higher than that in WT. QRT-PCR analysis revealed that ospuki mutants had higher transcription levels of ABA synthesis-related genes OsNCED2, OsNCED3, OsNCED4, and OsZEP1. Furthermore, it was shown that ospuki mutants were more sensitive to fluridone (Flu) during seed germination and seedling growth than WT according to exogenous Flu treatment experiments. In short, our findings suggest that OsPUKI may positively regulates rice seed germination by influencing ABA synthesis.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>39580126</pmid><doi>10.1016/j.gene.2024.149118</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 2025-02, Vol.936, p.149118, Article 149118 |
| issn | 0378-1119 1879-0038 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3132366105 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ABA Abscisic Acid - metabolism Gene Expression Regulation, Plant Germination - genetics Mutation Oryza - genetics Oryza - growth & development Oryza - metabolism Oryza sativa OsPUKI pfkB Plant Proteins - genetics Plant Proteins - metabolism Pyridones Seed germination Seedlings - genetics Seedlings - growth & development Seeds - genetics Seeds - growth & development |
| title | OsPUKI, a PfkB protein, regulates seed germination in rice by influencing ABA synthesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T20%3A09%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=OsPUKI,%20a%20PfkB%20protein,%20regulates%20seed%20germination%20in%20rice%20by%20influencing%20ABA%20synthesis&rft.jtitle=Gene&rft.au=Bao,%20Yi&rft.date=2025-02-05&rft.volume=936&rft.spage=149118&rft.pages=149118-&rft.artnum=149118&rft.issn=0378-1119&rft.eissn=1879-0038&rft_id=info:doi/10.1016/j.gene.2024.149118&rft_dat=%3Cproquest_cross%3E3132366105%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3132366105&rft_id=info:pmid/39580126&rft_els_id=S0378111924009995&rfr_iscdi=true |