Cspg4 sculpts oligodendrocyte precursor cell morphology
The extracellular matrix (ECM) provides critical biochemical and structural cues that regulate neural development. Chondroitin sulfate proteoglycans (CSPGs), a major ECM component, have been implicated in modulating oligodendrocyte precursor cell (OPC) proliferation, migration, and maturation, but t...
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| Veröffentlicht in: | Differentiation (London) 2024-11, Vol.140, p.100819, Article 100819 |
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| creator | Bromley-Coolidge, Samantha Iruegas, Diego Appel, Bruce |
| description | The extracellular matrix (ECM) provides critical biochemical and structural cues that regulate neural development. Chondroitin sulfate proteoglycans (CSPGs), a major ECM component, have been implicated in modulating oligodendrocyte precursor cell (OPC) proliferation, migration, and maturation, but their specific roles in oligodendrocyte lineage cell (OLC) development and myelination in vivo remain poorly understood. Here, we use zebrafish as a model system to investigate the spatiotemporal dynamics of ECM deposition and CSPG localization during central nervous system (CNS) development, with a focus on their relationship to OLCs. We demonstrate that ECM components, including CSPGs, are dynamically expressed in distinct spatiotemporal patterns coinciding with OLC development and myelination. We found that zebrafish lacking cspg4 function produced normal numbers of OLCs, which appeared to undergo proper differentiation. However, OPC morphology in mutant larvae was aberrant. Nevertheless, the number and length of myelin sheaths produced by mature oligodendrocytes were unaffected. These data indicate that Cspg4 regulates OPC morphogenesis in vivo, supporting the role of the ECM in neural development. |
| doi_str_mv | 10.1016/j.diff.2024.100819 |
| format | Article |
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Chondroitin sulfate proteoglycans (CSPGs), a major ECM component, have been implicated in modulating oligodendrocyte precursor cell (OPC) proliferation, migration, and maturation, but their specific roles in oligodendrocyte lineage cell (OLC) development and myelination in vivo remain poorly understood. Here, we use zebrafish as a model system to investigate the spatiotemporal dynamics of ECM deposition and CSPG localization during central nervous system (CNS) development, with a focus on their relationship to OLCs. We demonstrate that ECM components, including CSPGs, are dynamically expressed in distinct spatiotemporal patterns coinciding with OLC development and myelination. We found that zebrafish lacking cspg4 function produced normal numbers of OLCs, which appeared to undergo proper differentiation. However, OPC morphology in mutant larvae was aberrant. Nevertheless, the number and length of myelin sheaths produced by mature oligodendrocytes were unaffected. 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Chondroitin sulfate proteoglycans (CSPGs), a major ECM component, have been implicated in modulating oligodendrocyte precursor cell (OPC) proliferation, migration, and maturation, but their specific roles in oligodendrocyte lineage cell (OLC) development and myelination in vivo remain poorly understood. Here, we use zebrafish as a model system to investigate the spatiotemporal dynamics of ECM deposition and CSPG localization during central nervous system (CNS) development, with a focus on their relationship to OLCs. We demonstrate that ECM components, including CSPGs, are dynamically expressed in distinct spatiotemporal patterns coinciding with OLC development and myelination. We found that zebrafish lacking cspg4 function produced normal numbers of OLCs, which appeared to undergo proper differentiation. However, OPC morphology in mutant larvae was aberrant. Nevertheless, the number and length of myelin sheaths produced by mature oligodendrocytes were unaffected. These data indicate that Cspg4 regulates OPC morphogenesis in vivo, supporting the role of the ECM in neural development.</description><subject>Animals</subject><subject>Antigens</subject><subject>Cell Differentiation</subject><subject>Central Nervous System - cytology</subject><subject>Central Nervous System - growth & development</subject><subject>Central Nervous System - metabolism</subject><subject>Chondroitin Sulfate Proteoglycans - genetics</subject><subject>Chondroitin Sulfate Proteoglycans - metabolism</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - genetics</subject><subject>Extracellular Matrix - metabolism</subject><subject>Glia</subject><subject>Myelin Sheath - metabolism</subject><subject>Myelination</subject><subject>Neural cell differentiation</subject><subject>Neural cell fate</subject><subject>Oligodendrocyte Precursor Cells - cytology</subject><subject>Oligodendrocyte Precursor Cells - metabolism</subject><subject>Oligodendroglia - cytology</subject><subject>Oligodendroglia - metabolism</subject><subject>Proteoglycans</subject><subject>Zebrafish</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish Proteins - genetics</subject><subject>Zebrafish Proteins - metabolism</subject><issn>0301-4681</issn><issn>1432-0436</issn><issn>1432-0436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMouq7-AQ_So5eumSRNG_Aii1-w4EXPoSaTtUt3U5NW2H9vSlePngaG532ZeQi5AroACvJ2s7CNcwtGmUgLWoE6IjMQnOVUcHlMZpRTyIWs4Iycx7ihiZEMTskZV4WUoNSMlMvYrUUWzdB2fcx826y9xZ0N3ux7zLqAZgjRh8xg22ZbH7pP3_r1_oKcuLqNeHmYc_L--PC2fM5Xr08vy_tVblgFfY6AheFVaZ0rHNRVIQSqggEVtlSsEukgVMqWwBVzzikqCldIAcrWpSh4xefkZurtgv8aMPZ628TxlnqHfoiaAwehqpLLhLIJNcHHGNDpLjTbOuw1UD0K0xs9CtOjMD0JS6HrQ__wsUX7F_k1lIC7CcD05XeDQUfT4M6gbZKbXlvf_Nf_A96Wesw</recordid><startdate>202411</startdate><enddate>202411</enddate><creator>Bromley-Coolidge, Samantha</creator><creator>Iruegas, Diego</creator><creator>Appel, Bruce</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5579-9484</orcidid><orcidid>https://orcid.org/0000-0003-4500-0672</orcidid></search><sort><creationdate>202411</creationdate><title>Cspg4 sculpts oligodendrocyte precursor cell morphology</title><author>Bromley-Coolidge, Samantha ; Iruegas, Diego ; Appel, Bruce</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-e1e5c387dff5f1a8544e952104d79284086e99d71392fff9045f56419da745383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Cell Differentiation</topic><topic>Central Nervous System - cytology</topic><topic>Central Nervous System - growth & development</topic><topic>Central Nervous System - metabolism</topic><topic>Chondroitin Sulfate Proteoglycans - genetics</topic><topic>Chondroitin Sulfate Proteoglycans - metabolism</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - genetics</topic><topic>Extracellular Matrix - metabolism</topic><topic>Glia</topic><topic>Myelin Sheath - metabolism</topic><topic>Myelination</topic><topic>Neural cell differentiation</topic><topic>Neural cell fate</topic><topic>Oligodendrocyte Precursor Cells - cytology</topic><topic>Oligodendrocyte Precursor Cells - metabolism</topic><topic>Oligodendroglia - cytology</topic><topic>Oligodendroglia - metabolism</topic><topic>Proteoglycans</topic><topic>Zebrafish</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish Proteins - genetics</topic><topic>Zebrafish Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bromley-Coolidge, Samantha</creatorcontrib><creatorcontrib>Iruegas, Diego</creatorcontrib><creatorcontrib>Appel, Bruce</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Differentiation (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bromley-Coolidge, Samantha</au><au>Iruegas, Diego</au><au>Appel, Bruce</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cspg4 sculpts oligodendrocyte precursor cell morphology</atitle><jtitle>Differentiation (London)</jtitle><addtitle>Differentiation</addtitle><date>2024-11</date><risdate>2024</risdate><volume>140</volume><spage>100819</spage><pages>100819-</pages><artnum>100819</artnum><issn>0301-4681</issn><issn>1432-0436</issn><eissn>1432-0436</eissn><abstract>The extracellular matrix (ECM) provides critical biochemical and structural cues that regulate neural development. Chondroitin sulfate proteoglycans (CSPGs), a major ECM component, have been implicated in modulating oligodendrocyte precursor cell (OPC) proliferation, migration, and maturation, but their specific roles in oligodendrocyte lineage cell (OLC) development and myelination in vivo remain poorly understood. Here, we use zebrafish as a model system to investigate the spatiotemporal dynamics of ECM deposition and CSPG localization during central nervous system (CNS) development, with a focus on their relationship to OLCs. We demonstrate that ECM components, including CSPGs, are dynamically expressed in distinct spatiotemporal patterns coinciding with OLC development and myelination. We found that zebrafish lacking cspg4 function produced normal numbers of OLCs, which appeared to undergo proper differentiation. However, OPC morphology in mutant larvae was aberrant. Nevertheless, the number and length of myelin sheaths produced by mature oligodendrocytes were unaffected. 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| subjects | Animals Antigens Cell Differentiation Central Nervous System - cytology Central Nervous System - growth & development Central Nervous System - metabolism Chondroitin Sulfate Proteoglycans - genetics Chondroitin Sulfate Proteoglycans - metabolism Extracellular matrix Extracellular Matrix - genetics Extracellular Matrix - metabolism Glia Myelin Sheath - metabolism Myelination Neural cell differentiation Neural cell fate Oligodendrocyte Precursor Cells - cytology Oligodendrocyte Precursor Cells - metabolism Oligodendroglia - cytology Oligodendroglia - metabolism Proteoglycans Zebrafish Zebrafish - genetics Zebrafish Proteins - genetics Zebrafish Proteins - metabolism |
| title | Cspg4 sculpts oligodendrocyte precursor cell morphology |
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