Formulation and Characterization of RBCS Coated Carboplatin Loaded Nano‐Liposomal Formulation for Managing Breast Cancer
ABSTRACT Cell membrane‐coated Nano‐Liposomes (CM‐NLPs) offer a promising approach that combines the advantages of both host cells and synthetic nano‐liposomes (NLPs). This technique involves coating liposomes with red blood cell (RBC) membranes to enhance their functionality. In this study, novel ca...
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| Veröffentlicht in: | Drug development research 2024-12, Vol.85 (8), p.e70019-n/a |
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| creator | Dubey, Akhilesh Raju, Faby Lobo, Cynthia Lizzie Gs, Ravi Hebbar, Srinivas Shetty, Amitha Kumar, Pankaj El‐Zahaby, Sally A. |
| description | ABSTRACT
Cell membrane‐coated Nano‐Liposomes (CM‐NLPs) offer a promising approach that combines the advantages of both host cells and synthetic nano‐liposomes (NLPs). This technique involves coating liposomes with red blood cell (RBC) membranes to enhance their functionality. In this study, novel carboplatin‐loaded NLPs (CP‐NLPs) were formulated using phospholipids (Soya Phosphatidyl Choline) and cholesterol through the thin‐film hydration method, and optimized using a 32 full factorial design. The optimized CP‐NLPs were coated with RBC membranes, resulting in the formulation “CP‐RBCs‐NLPs.” These were characterized for particle size, zeta potential, entrapment efficiency, transmission electron microscopy (TEM), differential scanning calorimetry (DSC), protein content, in vitro drug release, cell viability, and stability. The optimized CP‐RBCs‐NLPs exhibited a particle size of 103.6 nm, with zeta potential values of −27.3 mV indicating good stability. The entrapment efficiency was approximately 56%, and the drug release profile showed sustained release for up to 8 h. Cytotoxicity studies in human triple‐negative breast cancer (MDA‐MB468) cell lines demonstrated that CP‐RBCs‐NLPs effectively delivered the drug into target cells, facilitating cell death due to their bilayer structure similar to cell membranes. Overall, CP‐RBCs‐NLPs outperformed both carboplatin‐loaded conventional NLPs (CP‐CNLPs) and carboplatin‐conventional solution (CP‐CNS), making it a superior formulation for drug delivery. |
| doi_str_mv | 10.1002/ddr.70019 |
| format | Article |
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Cell membrane‐coated Nano‐Liposomes (CM‐NLPs) offer a promising approach that combines the advantages of both host cells and synthetic nano‐liposomes (NLPs). This technique involves coating liposomes with red blood cell (RBC) membranes to enhance their functionality. In this study, novel carboplatin‐loaded NLPs (CP‐NLPs) were formulated using phospholipids (Soya Phosphatidyl Choline) and cholesterol through the thin‐film hydration method, and optimized using a 32 full factorial design. The optimized CP‐NLPs were coated with RBC membranes, resulting in the formulation “CP‐RBCs‐NLPs.” These were characterized for particle size, zeta potential, entrapment efficiency, transmission electron microscopy (TEM), differential scanning calorimetry (DSC), protein content, in vitro drug release, cell viability, and stability. The optimized CP‐RBCs‐NLPs exhibited a particle size of 103.6 nm, with zeta potential values of −27.3 mV indicating good stability. The entrapment efficiency was approximately 56%, and the drug release profile showed sustained release for up to 8 h. Cytotoxicity studies in human triple‐negative breast cancer (MDA‐MB468) cell lines demonstrated that CP‐RBCs‐NLPs effectively delivered the drug into target cells, facilitating cell death due to their bilayer structure similar to cell membranes. Overall, CP‐RBCs‐NLPs outperformed both carboplatin‐loaded conventional NLPs (CP‐CNLPs) and carboplatin‐conventional solution (CP‐CNS), making it a superior formulation for drug delivery.</description><identifier>ISSN: 0272-4391</identifier><identifier>ISSN: 1098-2299</identifier><identifier>EISSN: 1098-2299</identifier><identifier>DOI: 10.1002/ddr.70019</identifier><identifier>PMID: 39558836</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Antineoplastic Agents - administration & dosage ; Antineoplastic Agents - chemistry ; Antineoplastic Agents - pharmacology ; Breast cancer ; Breast Neoplasms - drug therapy ; Calorimetry ; Carboplatin ; Carboplatin - administration & dosage ; Carboplatin - chemistry ; Carboplatin - pharmacology ; Cell death ; Cell Line, Tumor ; Cell membranes ; Cell Survival - drug effects ; Cell viability ; Cholesterol ; Choline ; Coatings ; Controlled release ; Cytotoxicity ; Differential scanning calorimetry ; Drug delivery ; Drug Liberation ; Entrapment ; Erythrocytes ; Erythrocytes - drug effects ; Factorial design ; Female ; Humans ; Liposomes ; Membranes ; Nanoparticles - administration & dosage ; Nanoparticles - chemistry ; nano‐liposomes ; Particle Size ; Phosphatidylcholine ; Phospholipids ; RBCs ; Stability ; Sustained release ; Thin films ; Transmission electron microscopy ; Tumor cell lines ; vesicular system ; Zeta potential</subject><ispartof>Drug development research, 2024-12, Vol.85 (8), p.e70019-n/a</ispartof><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2439-319aeb02b0ed5f9514f33902eba374b7662067f8e8ed143e86084f11fb6f34893</cites><orcidid>0000-0002-8751-990X ; 0000-0002-4834-2849</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fddr.70019$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fddr.70019$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39558836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dubey, Akhilesh</creatorcontrib><creatorcontrib>Raju, Faby</creatorcontrib><creatorcontrib>Lobo, Cynthia Lizzie</creatorcontrib><creatorcontrib>Gs, Ravi</creatorcontrib><creatorcontrib>Hebbar, Srinivas</creatorcontrib><creatorcontrib>Shetty, Amitha</creatorcontrib><creatorcontrib>Kumar, Pankaj</creatorcontrib><creatorcontrib>El‐Zahaby, Sally A.</creatorcontrib><title>Formulation and Characterization of RBCS Coated Carboplatin Loaded Nano‐Liposomal Formulation for Managing Breast Cancer</title><title>Drug development research</title><addtitle>Drug Dev Res</addtitle><description>ABSTRACT
Cell membrane‐coated Nano‐Liposomes (CM‐NLPs) offer a promising approach that combines the advantages of both host cells and synthetic nano‐liposomes (NLPs). This technique involves coating liposomes with red blood cell (RBC) membranes to enhance their functionality. In this study, novel carboplatin‐loaded NLPs (CP‐NLPs) were formulated using phospholipids (Soya Phosphatidyl Choline) and cholesterol through the thin‐film hydration method, and optimized using a 32 full factorial design. The optimized CP‐NLPs were coated with RBC membranes, resulting in the formulation “CP‐RBCs‐NLPs.” These were characterized for particle size, zeta potential, entrapment efficiency, transmission electron microscopy (TEM), differential scanning calorimetry (DSC), protein content, in vitro drug release, cell viability, and stability. The optimized CP‐RBCs‐NLPs exhibited a particle size of 103.6 nm, with zeta potential values of −27.3 mV indicating good stability. The entrapment efficiency was approximately 56%, and the drug release profile showed sustained release for up to 8 h. Cytotoxicity studies in human triple‐negative breast cancer (MDA‐MB468) cell lines demonstrated that CP‐RBCs‐NLPs effectively delivered the drug into target cells, facilitating cell death due to their bilayer structure similar to cell membranes. Overall, CP‐RBCs‐NLPs outperformed both carboplatin‐loaded conventional NLPs (CP‐CNLPs) and carboplatin‐conventional solution (CP‐CNS), making it a superior formulation for drug delivery.</description><subject>Antineoplastic Agents - administration & dosage</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Calorimetry</subject><subject>Carboplatin</subject><subject>Carboplatin - administration & dosage</subject><subject>Carboplatin - chemistry</subject><subject>Carboplatin - pharmacology</subject><subject>Cell death</subject><subject>Cell Line, Tumor</subject><subject>Cell membranes</subject><subject>Cell Survival - drug effects</subject><subject>Cell viability</subject><subject>Cholesterol</subject><subject>Choline</subject><subject>Coatings</subject><subject>Controlled release</subject><subject>Cytotoxicity</subject><subject>Differential scanning calorimetry</subject><subject>Drug delivery</subject><subject>Drug Liberation</subject><subject>Entrapment</subject><subject>Erythrocytes</subject><subject>Erythrocytes - drug effects</subject><subject>Factorial design</subject><subject>Female</subject><subject>Humans</subject><subject>Liposomes</subject><subject>Membranes</subject><subject>Nanoparticles - administration & dosage</subject><subject>Nanoparticles - chemistry</subject><subject>nano‐liposomes</subject><subject>Particle Size</subject><subject>Phosphatidylcholine</subject><subject>Phospholipids</subject><subject>RBCs</subject><subject>Stability</subject><subject>Sustained release</subject><subject>Thin films</subject><subject>Transmission electron microscopy</subject><subject>Tumor cell lines</subject><subject>vesicular system</subject><subject>Zeta potential</subject><issn>0272-4391</issn><issn>1098-2299</issn><issn>1098-2299</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1K3kAUhodiqZ_aRW-gBLrRRXT-kswsa9RW-FTQdh1OkjM2ksx8ziSIrrwEr9ErcTS2SMHVgfc85-HAS8gXRncZpXyvbf1uQSnTH8iCUa1SzrVeIwvKC55Kodk62QjhKhJMKvWJrAudZUqJfEHujpwfph7GztkEbJuUf8BDM6Lv7ubQmeR8v7xISgcjxj342q2eD2yydNDG6BSse7x_WHYrF9wAffLWaZxPTsDCZWcvk32PEMbosA36LfLRQB_w8-vcJL-PDn-VP9Pl2Y_j8vsybXj8PRVMA9aU1xTbzOiMSSOEphxrEIWsizznNC-MQoUtkwJVTpU0jJk6N0IqLTbJ9uxdeXc9YRiroQsN9j1YdFOoBBOUU6WVjOi3_9ArN3kbv4uUlFrRTPFI7cxU410IHk218t0A_rZitHoupIqFVC-FRPbrq3GqB2z_kX8biMDeDNx0Pd6-b6oODs5n5RP0F5UG</recordid><startdate>202412</startdate><enddate>202412</enddate><creator>Dubey, Akhilesh</creator><creator>Raju, Faby</creator><creator>Lobo, Cynthia Lizzie</creator><creator>Gs, Ravi</creator><creator>Hebbar, Srinivas</creator><creator>Shetty, Amitha</creator><creator>Kumar, Pankaj</creator><creator>El‐Zahaby, Sally A.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8751-990X</orcidid><orcidid>https://orcid.org/0000-0002-4834-2849</orcidid></search><sort><creationdate>202412</creationdate><title>Formulation and Characterization of RBCS Coated Carboplatin Loaded Nano‐Liposomal Formulation for Managing Breast Cancer</title><author>Dubey, Akhilesh ; 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Cell membrane‐coated Nano‐Liposomes (CM‐NLPs) offer a promising approach that combines the advantages of both host cells and synthetic nano‐liposomes (NLPs). This technique involves coating liposomes with red blood cell (RBC) membranes to enhance their functionality. In this study, novel carboplatin‐loaded NLPs (CP‐NLPs) were formulated using phospholipids (Soya Phosphatidyl Choline) and cholesterol through the thin‐film hydration method, and optimized using a 32 full factorial design. The optimized CP‐NLPs were coated with RBC membranes, resulting in the formulation “CP‐RBCs‐NLPs.” These were characterized for particle size, zeta potential, entrapment efficiency, transmission electron microscopy (TEM), differential scanning calorimetry (DSC), protein content, in vitro drug release, cell viability, and stability. The optimized CP‐RBCs‐NLPs exhibited a particle size of 103.6 nm, with zeta potential values of −27.3 mV indicating good stability. The entrapment efficiency was approximately 56%, and the drug release profile showed sustained release for up to 8 h. Cytotoxicity studies in human triple‐negative breast cancer (MDA‐MB468) cell lines demonstrated that CP‐RBCs‐NLPs effectively delivered the drug into target cells, facilitating cell death due to their bilayer structure similar to cell membranes. Overall, CP‐RBCs‐NLPs outperformed both carboplatin‐loaded conventional NLPs (CP‐CNLPs) and carboplatin‐conventional solution (CP‐CNS), making it a superior formulation for drug delivery.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>39558836</pmid><doi>10.1002/ddr.70019</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-8751-990X</orcidid><orcidid>https://orcid.org/0000-0002-4834-2849</orcidid></addata></record> |
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| subjects | Antineoplastic Agents - administration & dosage Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology Breast cancer Breast Neoplasms - drug therapy Calorimetry Carboplatin Carboplatin - administration & dosage Carboplatin - chemistry Carboplatin - pharmacology Cell death Cell Line, Tumor Cell membranes Cell Survival - drug effects Cell viability Cholesterol Choline Coatings Controlled release Cytotoxicity Differential scanning calorimetry Drug delivery Drug Liberation Entrapment Erythrocytes Erythrocytes - drug effects Factorial design Female Humans Liposomes Membranes Nanoparticles - administration & dosage Nanoparticles - chemistry nano‐liposomes Particle Size Phosphatidylcholine Phospholipids RBCs Stability Sustained release Thin films Transmission electron microscopy Tumor cell lines vesicular system Zeta potential |
| title | Formulation and Characterization of RBCS Coated Carboplatin Loaded Nano‐Liposomal Formulation for Managing Breast Cancer |
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