A histological procedure for the study of insect chitinized tissues in light microscopy

We present a procedure to obtain histological preparations of insect cuticles for their observation in ligth microscopy. The use of EDTA (ethylendiaminetetraacetic acid), as a chelator of certain metals present in chitin, soften the tissues and allows us to cut them. The tissues were embbeding in pl...

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Veröffentlicht in:	Micron (Oxford, England : 1993) England : 1993), 2025-01, Vol.188, p.103744, Article 103744
Hauptverfasser:	Gallardo, Manuela, Valladolid, María
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals chelating agents Chitin Chitin - chemistry Cuticle Edetic Acid - pharmacology EDTA Histological Techniques - methods histology Insect Insecta - ultrastructure insects Integument light microscopes Light microscopy Microscopy - methods Microtomy - methods
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