Diverse effects of fluorescent labels on alpha-synuclein condensate formation during liquid-liquid phase separation

Liquid-liquid phase separation is an emerging field of study, dedicated to understanding the mechanism and role of biomolecule assembly into membraneless organelles. One of the main methods employed in studying protein and nucleic acid droplet formation is fluorescence microscopy. Despite functionin...

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Veröffentlicht in:	International journal of biological macromolecules 2024-12, Vol.283 (Pt 2), p.137688, Article 137688
Hauptverfasser:	Ziaunys, Mantas, Sulskis, Darius, Veiveris, Dominykas, Sakalauskas, Andrius, Mikalauskaite, Kamile, Smirnovas, Vytautas
Format:	Artikel
Sprache:	eng
Schlagworte:	Alpha-synuclein alpha-Synuclein - chemistry alpha-Synuclein - metabolism Biomolecular Condensates - chemistry Biomolecular Condensates - metabolism Droplet size Fluorescent Dyes - chemistry Fluorescent labels Green Fluorescent Proteins - metabolism Humans Liquid-liquid phase separation Luminescent Proteins - chemistry Luminescent Proteins - genetics Luminescent Proteins - metabolism Microscopy, Fluorescence Phase Separation Protein Aggregates Protein condensates
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