Methicillin- and vancomycin-resistant Staphylococcus aureus in chicken carcasses, ready-to-eat chicken meat sandwiches, and buffalo milk

Methicillin-resistant Staphylococcus aureus (MRSA) is a growing public health concern; however, there is limited information about MRSA and VRSA (Vancomycin-resistant S. aureus) among animal-origin food. Therefore, this study intended to elucidate the prevalence, enterotoxin existence, antimicrobial...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of food microbiology 2025-01, Vol.427, p.110968, Article 110968
Hauptverfasser: Elshebrawy, Hend Ali, Kasem, Nahed Gomaa, Sallam, Khalid Ibrahim
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page 110968
container_title International journal of food microbiology
container_volume 427
creator Elshebrawy, Hend Ali
Kasem, Nahed Gomaa
Sallam, Khalid Ibrahim
description Methicillin-resistant Staphylococcus aureus (MRSA) is a growing public health concern; however, there is limited information about MRSA and VRSA (Vancomycin-resistant S. aureus) among animal-origin food. Therefore, this study intended to elucidate the prevalence, enterotoxin existence, antimicrobial resistance profiles, and antimicrobial resistance genes of S. aureus strains isolated from chicken carcasses, ready-to-eat (RTE) chicken meat sandwiches, and buffalo milk samples marketed in Mansoura City, Egypt. Of the 240 samples examined, 52.1 % were contaminated with S. aureus, with a mean count of 4.11 log10 CFU/g. A total of 250 isolates were verified as S. aureus by PCR targeting nuc gene, of which 39.2 % (98/250) harbored at least one S. aureus enterotoxin (SE) gene. The predominant SE genes were sea (61.2 %, 60/98), followed by sed (58.2 %, 57/98), sec (38.8 %, 38/98), and seb (27.6 %, 27/98). All isolates were resistant to at least one antimicrobial, with an average MAR (multiple antibiotic resistance) index of 0.530. Four isolates exhibited resistance to all antimicrobial agents tested. Interestingly, 100 %, 76.4 %, 35.6 %, 30.8, 10.4 %, 6 %, and 1.6 % of isolates were resistant to penicillin, azithromycin, oxacillin, ceftaroline, vancomycin, linezolid, and daptomycin, respectively. Of the 250 S. aureus strains tested, 38 % were confirmed as MRSA by mecA gene, while 10.4 % were identified as VRSA by vanA gene. The high prevalence of MRSA and VRSA isolates among samples tested is worrisome. Hence, monitoring antimicrobial usage in veterinary medicine and applying strict hygienic measures during food handling and processing is imperative to prevent the spread of such resistant bacteria and protect public health. [Display omitted] •52.1 % of examined poultry and dairy samples were contaminated with S. aureus.•88.8 % of S. aureus isolates were multidrug-resistant and 39.2 % were enterotoxigenic.•38 % of S. aureus strains were confirmed as MRSA by the mecA gene.•The sea was the most common staphylococcal enterotoxins genes.•10.4 % of the isolates were vancomycin-resistant and harbored vanA gene.
doi_str_mv 10.1016/j.ijfoodmicro.2024.110968
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3128980823</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168160524004124</els_id><sourcerecordid>3128980823</sourcerecordid><originalsourceid>FETCH-LOGICAL-c251t-ac11d055f9623e98553350c78deb13ce3ea53b770f1ff087dd03714f3a3817f33</originalsourceid><addsrcrecordid>eNqNkcFu3CAQhlHVqtmmfYXIvfUQb8AsNhyrVZJWSpVDkzNih0HLxjZbwKn2DfLYwdok6rGnnxHfzK-Zn5CvjC4ZZe3Fbul3LgQ7eIhh2dBmtWSMqla-IwsmO1XzVUvfk0VhZc1aKk7Ip5R2lFLBOf1ITrgSq1YqtSBPvzBvPfi-92NdmdFWj2aEMByg1BGTT9mMufqdzX576AMEgClVZopYxI8VlOYHLGoimJQwnVcRjT3UOdRo8tv_MBepzP_rYTtTs9Vmcs70oRp8__CZfCjvhF9e9JTcX13erX_UN7fXP9ffb2poBMu1AcYsFcKptuGopCgbCQqdtLhhHJCjEXzTddQx56jsrKW8YyvHDZesc5yfkm_HufsY_kyYsh58Aux7M2KYkuaskUpS2cyoOqLlyilFdHof_WDiQTOq5yD0Tv8ThJ6D0McgSu_Zi820GdC-db5evgDrI4Bl2UePUSfwOAJaHxGytsH_h80zK7aiMQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3128980823</pqid></control><display><type>article</type><title>Methicillin- and vancomycin-resistant Staphylococcus aureus in chicken carcasses, ready-to-eat chicken meat sandwiches, and buffalo milk</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Elshebrawy, Hend Ali ; Kasem, Nahed Gomaa ; Sallam, Khalid Ibrahim</creator><creatorcontrib>Elshebrawy, Hend Ali ; Kasem, Nahed Gomaa ; Sallam, Khalid Ibrahim</creatorcontrib><description>Methicillin-resistant Staphylococcus aureus (MRSA) is a growing public health concern; however, there is limited information about MRSA and VRSA (Vancomycin-resistant S. aureus) among animal-origin food. Therefore, this study intended to elucidate the prevalence, enterotoxin existence, antimicrobial resistance profiles, and antimicrobial resistance genes of S. aureus strains isolated from chicken carcasses, ready-to-eat (RTE) chicken meat sandwiches, and buffalo milk samples marketed in Mansoura City, Egypt. Of the 240 samples examined, 52.1 % were contaminated with S. aureus, with a mean count of 4.11 log10 CFU/g. A total of 250 isolates were verified as S. aureus by PCR targeting nuc gene, of which 39.2 % (98/250) harbored at least one S. aureus enterotoxin (SE) gene. The predominant SE genes were sea (61.2 %, 60/98), followed by sed (58.2 %, 57/98), sec (38.8 %, 38/98), and seb (27.6 %, 27/98). All isolates were resistant to at least one antimicrobial, with an average MAR (multiple antibiotic resistance) index of 0.530. Four isolates exhibited resistance to all antimicrobial agents tested. Interestingly, 100 %, 76.4 %, 35.6 %, 30.8, 10.4 %, 6 %, and 1.6 % of isolates were resistant to penicillin, azithromycin, oxacillin, ceftaroline, vancomycin, linezolid, and daptomycin, respectively. Of the 250 S. aureus strains tested, 38 % were confirmed as MRSA by mecA gene, while 10.4 % were identified as VRSA by vanA gene. The high prevalence of MRSA and VRSA isolates among samples tested is worrisome. Hence, monitoring antimicrobial usage in veterinary medicine and applying strict hygienic measures during food handling and processing is imperative to prevent the spread of such resistant bacteria and protect public health. [Display omitted] •52.1 % of examined poultry and dairy samples were contaminated with S. aureus.•88.8 % of S. aureus isolates were multidrug-resistant and 39.2 % were enterotoxigenic.•38 % of S. aureus strains were confirmed as MRSA by the mecA gene.•The sea was the most common staphylococcal enterotoxins genes.•10.4 % of the isolates were vancomycin-resistant and harbored vanA gene.</description><identifier>ISSN: 0168-1605</identifier><identifier>ISSN: 1879-3460</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2024.110968</identifier><identifier>PMID: 39546899</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antimicrobial resistance ; Buffalo milk ; Buffaloes ; Chicken sandwiches ; Chickens - microbiology ; Egypt ; Enterotoxin genes ; Enterotoxins - genetics ; Food Contamination - analysis ; Food Microbiology ; Methicillin-resistant Staphylococcus aureus (MRSA) ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - isolation &amp; purification ; Microbial Sensitivity Tests ; Milk - microbiology ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation &amp; purification ; Vancomycin Resistance - genetics ; Vancomycin-resistant Staphylococcus aureus (VRSA)</subject><ispartof>International journal of food microbiology, 2025-01, Vol.427, p.110968, Article 110968</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c251t-ac11d055f9623e98553350c78deb13ce3ea53b770f1ff087dd03714f3a3817f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2024.110968$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39546899$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elshebrawy, Hend Ali</creatorcontrib><creatorcontrib>Kasem, Nahed Gomaa</creatorcontrib><creatorcontrib>Sallam, Khalid Ibrahim</creatorcontrib><title>Methicillin- and vancomycin-resistant Staphylococcus aureus in chicken carcasses, ready-to-eat chicken meat sandwiches, and buffalo milk</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Methicillin-resistant Staphylococcus aureus (MRSA) is a growing public health concern; however, there is limited information about MRSA and VRSA (Vancomycin-resistant S. aureus) among animal-origin food. Therefore, this study intended to elucidate the prevalence, enterotoxin existence, antimicrobial resistance profiles, and antimicrobial resistance genes of S. aureus strains isolated from chicken carcasses, ready-to-eat (RTE) chicken meat sandwiches, and buffalo milk samples marketed in Mansoura City, Egypt. Of the 240 samples examined, 52.1 % were contaminated with S. aureus, with a mean count of 4.11 log10 CFU/g. A total of 250 isolates were verified as S. aureus by PCR targeting nuc gene, of which 39.2 % (98/250) harbored at least one S. aureus enterotoxin (SE) gene. The predominant SE genes were sea (61.2 %, 60/98), followed by sed (58.2 %, 57/98), sec (38.8 %, 38/98), and seb (27.6 %, 27/98). All isolates were resistant to at least one antimicrobial, with an average MAR (multiple antibiotic resistance) index of 0.530. Four isolates exhibited resistance to all antimicrobial agents tested. Interestingly, 100 %, 76.4 %, 35.6 %, 30.8, 10.4 %, 6 %, and 1.6 % of isolates were resistant to penicillin, azithromycin, oxacillin, ceftaroline, vancomycin, linezolid, and daptomycin, respectively. Of the 250 S. aureus strains tested, 38 % were confirmed as MRSA by mecA gene, while 10.4 % were identified as VRSA by vanA gene. The high prevalence of MRSA and VRSA isolates among samples tested is worrisome. Hence, monitoring antimicrobial usage in veterinary medicine and applying strict hygienic measures during food handling and processing is imperative to prevent the spread of such resistant bacteria and protect public health. [Display omitted] •52.1 % of examined poultry and dairy samples were contaminated with S. aureus.•88.8 % of S. aureus isolates were multidrug-resistant and 39.2 % were enterotoxigenic.•38 % of S. aureus strains were confirmed as MRSA by the mecA gene.•The sea was the most common staphylococcal enterotoxins genes.•10.4 % of the isolates were vancomycin-resistant and harbored vanA gene.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimicrobial resistance</subject><subject>Buffalo milk</subject><subject>Buffaloes</subject><subject>Chicken sandwiches</subject><subject>Chickens - microbiology</subject><subject>Egypt</subject><subject>Enterotoxin genes</subject><subject>Enterotoxins - genetics</subject><subject>Food Contamination - analysis</subject><subject>Food Microbiology</subject><subject>Methicillin-resistant Staphylococcus aureus (MRSA)</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - isolation &amp; purification</subject><subject>Microbial Sensitivity Tests</subject><subject>Milk - microbiology</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation &amp; purification</subject><subject>Vancomycin Resistance - genetics</subject><subject>Vancomycin-resistant Staphylococcus aureus (VRSA)</subject><issn>0168-1605</issn><issn>1879-3460</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu3CAQhlHVqtmmfYXIvfUQb8AsNhyrVZJWSpVDkzNih0HLxjZbwKn2DfLYwdok6rGnnxHfzK-Zn5CvjC4ZZe3Fbul3LgQ7eIhh2dBmtWSMqla-IwsmO1XzVUvfk0VhZc1aKk7Ip5R2lFLBOf1ITrgSq1YqtSBPvzBvPfi-92NdmdFWj2aEMByg1BGTT9mMufqdzX576AMEgClVZopYxI8VlOYHLGoimJQwnVcRjT3UOdRo8tv_MBepzP_rYTtTs9Vmcs70oRp8__CZfCjvhF9e9JTcX13erX_UN7fXP9ffb2poBMu1AcYsFcKptuGopCgbCQqdtLhhHJCjEXzTddQx56jsrKW8YyvHDZesc5yfkm_HufsY_kyYsh58Aux7M2KYkuaskUpS2cyoOqLlyilFdHof_WDiQTOq5yD0Tv8ThJ6D0McgSu_Zi820GdC-db5evgDrI4Bl2UePUSfwOAJaHxGytsH_h80zK7aiMQ</recordid><startdate>20250116</startdate><enddate>20250116</enddate><creator>Elshebrawy, Hend Ali</creator><creator>Kasem, Nahed Gomaa</creator><creator>Sallam, Khalid Ibrahim</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20250116</creationdate><title>Methicillin- and vancomycin-resistant Staphylococcus aureus in chicken carcasses, ready-to-eat chicken meat sandwiches, and buffalo milk</title><author>Elshebrawy, Hend Ali ; Kasem, Nahed Gomaa ; Sallam, Khalid Ibrahim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c251t-ac11d055f9623e98553350c78deb13ce3ea53b770f1ff087dd03714f3a3817f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antimicrobial resistance</topic><topic>Buffalo milk</topic><topic>Buffaloes</topic><topic>Chicken sandwiches</topic><topic>Chickens - microbiology</topic><topic>Egypt</topic><topic>Enterotoxin genes</topic><topic>Enterotoxins - genetics</topic><topic>Food Contamination - analysis</topic><topic>Food Microbiology</topic><topic>Methicillin-resistant Staphylococcus aureus (MRSA)</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - isolation &amp; purification</topic><topic>Microbial Sensitivity Tests</topic><topic>Milk - microbiology</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation &amp; purification</topic><topic>Vancomycin Resistance - genetics</topic><topic>Vancomycin-resistant Staphylococcus aureus (VRSA)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Elshebrawy, Hend Ali</creatorcontrib><creatorcontrib>Kasem, Nahed Gomaa</creatorcontrib><creatorcontrib>Sallam, Khalid Ibrahim</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elshebrawy, Hend Ali</au><au>Kasem, Nahed Gomaa</au><au>Sallam, Khalid Ibrahim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methicillin- and vancomycin-resistant Staphylococcus aureus in chicken carcasses, ready-to-eat chicken meat sandwiches, and buffalo milk</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2025-01-16</date><risdate>2025</risdate><volume>427</volume><spage>110968</spage><pages>110968-</pages><artnum>110968</artnum><issn>0168-1605</issn><issn>1879-3460</issn><eissn>1879-3460</eissn><abstract>Methicillin-resistant Staphylococcus aureus (MRSA) is a growing public health concern; however, there is limited information about MRSA and VRSA (Vancomycin-resistant S. aureus) among animal-origin food. Therefore, this study intended to elucidate the prevalence, enterotoxin existence, antimicrobial resistance profiles, and antimicrobial resistance genes of S. aureus strains isolated from chicken carcasses, ready-to-eat (RTE) chicken meat sandwiches, and buffalo milk samples marketed in Mansoura City, Egypt. Of the 240 samples examined, 52.1 % were contaminated with S. aureus, with a mean count of 4.11 log10 CFU/g. A total of 250 isolates were verified as S. aureus by PCR targeting nuc gene, of which 39.2 % (98/250) harbored at least one S. aureus enterotoxin (SE) gene. The predominant SE genes were sea (61.2 %, 60/98), followed by sed (58.2 %, 57/98), sec (38.8 %, 38/98), and seb (27.6 %, 27/98). All isolates were resistant to at least one antimicrobial, with an average MAR (multiple antibiotic resistance) index of 0.530. Four isolates exhibited resistance to all antimicrobial agents tested. Interestingly, 100 %, 76.4 %, 35.6 %, 30.8, 10.4 %, 6 %, and 1.6 % of isolates were resistant to penicillin, azithromycin, oxacillin, ceftaroline, vancomycin, linezolid, and daptomycin, respectively. Of the 250 S. aureus strains tested, 38 % were confirmed as MRSA by mecA gene, while 10.4 % were identified as VRSA by vanA gene. The high prevalence of MRSA and VRSA isolates among samples tested is worrisome. Hence, monitoring antimicrobial usage in veterinary medicine and applying strict hygienic measures during food handling and processing is imperative to prevent the spread of such resistant bacteria and protect public health. [Display omitted] •52.1 % of examined poultry and dairy samples were contaminated with S. aureus.•88.8 % of S. aureus isolates were multidrug-resistant and 39.2 % were enterotoxigenic.•38 % of S. aureus strains were confirmed as MRSA by the mecA gene.•The sea was the most common staphylococcal enterotoxins genes.•10.4 % of the isolates were vancomycin-resistant and harbored vanA gene.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>39546899</pmid><doi>10.1016/j.ijfoodmicro.2024.110968</doi></addata></record>
fulltext fulltext
identifier ISSN: 0168-1605
ispartof International journal of food microbiology, 2025-01, Vol.427, p.110968, Article 110968
issn 0168-1605
1879-3460
1879-3460
language eng
recordid cdi_proquest_miscellaneous_3128980823
source MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects Animals
Anti-Bacterial Agents - pharmacology
Antimicrobial resistance
Buffalo milk
Buffaloes
Chicken sandwiches
Chickens - microbiology
Egypt
Enterotoxin genes
Enterotoxins - genetics
Food Contamination - analysis
Food Microbiology
Methicillin-resistant Staphylococcus aureus (MRSA)
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - genetics
Methicillin-Resistant Staphylococcus aureus - isolation & purification
Microbial Sensitivity Tests
Milk - microbiology
Staphylococcus aureus - drug effects
Staphylococcus aureus - genetics
Staphylococcus aureus - isolation & purification
Vancomycin Resistance - genetics
Vancomycin-resistant Staphylococcus aureus (VRSA)
title Methicillin- and vancomycin-resistant Staphylococcus aureus in chicken carcasses, ready-to-eat chicken meat sandwiches, and buffalo milk
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T02%3A15%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Methicillin-%20and%20vancomycin-resistant%20Staphylococcus%20aureus%20in%20chicken%20carcasses,%20ready-to-eat%20chicken%20meat%20sandwiches,%20and%20buffalo%20milk&rft.jtitle=International%20journal%20of%20food%20microbiology&rft.au=Elshebrawy,%20Hend%20Ali&rft.date=2025-01-16&rft.volume=427&rft.spage=110968&rft.pages=110968-&rft.artnum=110968&rft.issn=0168-1605&rft.eissn=1879-3460&rft_id=info:doi/10.1016/j.ijfoodmicro.2024.110968&rft_dat=%3Cproquest_cross%3E3128980823%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3128980823&rft_id=info:pmid/39546899&rft_els_id=S0168160524004124&rfr_iscdi=true