Metabolomics and transcriptomics analysis reveals the enhancement of growth, anti-oxidative stress and immunity by (-)-epigallocatechin-3-gallate in Litopenaeus vannamei

(−)-epigallocatechin-3-gallate (EGCG), the main active component of tea polyphenols, has been less studied in Litopenaeus vannamei. Therefore, the potential benefits of EGCG on L. vannamei were explored in this experiment. L. vannamei were fed diets containing EGCG (0, 0.5, and 1.0 g/kg). At the end of the 60-day farming experiment, metabolomics, transcriptomics, quantitative real-time PCR assays, and tissue sections were used to assess and explore the effects of EGCG on growth, antioxidant capacity, and immunity. It was found that the best growth and genes expressions related to antioxidant, anti-stress, apoptosis, and immunity were observed in the group with 0.5 g/kg EGCG. The analysis of hepatopancreatic metabolomics and transcriptomics results revealed bumper differential metabolites and differentially expressed genes (DEGs) associated with nutrient metabolism, digestion, immunity, and environmental adaptation. Many metabolites with anti-inflammatory, antibacterial, antiviral, and antioxidant activities have been identified. A large number of DEGs were enriched in apoptosis, Rap1 signaling pathway, HIF-1 signaling pathway, hippo signaling pathway, chemokine signaling pathway, and signaling pathways related to amino acid and lipid metabolism. In conclusion, dietary 0.5 g/kg EGCG was beneficial for growth, resistance to oxidative stress, and immunity in L. vannamei. The improvement of the properties was attributed to the modulation of signaling pathways, metabolites abundance and genes expressions related to the above properties by EGCG. •EGCG may involve in the regulation of immunity, apoptosis and nutrient metabolism.•Dietary addition of EGCG at 0.5 g/kg promoted the growth of Litopenaeus vannamei.•Addition of 0.5 g/kg EGCG to the diet can ameliorate the apoptosis of L. vannamei.•Diets with 0.5 g/kg EGCG can improve the antioxidant and immunity of L. vannamei.