Construction of engineered probiotic that adhere and display nanobody to neutralize porcine reproductive and respiratory syndrome virus
Pathogenic blue ear disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) bring severe loss to breeding industry due to high infectivity and mortality. L. plantarum serves as the probiotic host strain, known for its beneficial properties in the gut microbiota. E. coli is used...
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| Veröffentlicht in: | Archives of microbiology 2024-12, Vol.206 (12), p.466, Article 466 |
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| creator | Zhou, Li Zhou, Hanlin Wang, Panying Xu, Hang Wu, Jiayi Zhou, Yuanzhuo Feng, Jiaying Zheng, Weiyi |
| description | Pathogenic blue ear disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) bring severe loss to breeding industry due to high infectivity and mortality.
L. plantarum
serves as the probiotic host strain, known for its beneficial properties in the gut microbiota.
E. coli
is used as a cloning host for the initial genetic engineering steps, facilitating the construction and amplification of the desired genetic constructs. In this study, using synthetic biology technology, we constructed engineered probiotics which could adhere and display nanobody on the surface to neutralize virus. Firstly, we screen an optimal nanobody to effectively bind with PRRSV by building library, expression and purification. Then, the integration of adhesion protein and nanobody into the genome of probiotics significantly improved its adhesion to IPEC-J2 cells. In addition, this engineered probiotic is almost non-toxic to cells with good safety, which can be used as a daily probiotics to prevent virus fecal transmission. Our study proposed this novel construction strategy of engineering probiotics with both adhesion and neutralization effects, which provided a new therapeutic view for intestinal virus clearance.
Graphical Abstract |
| doi_str_mv | 10.1007/s00203-024-04198-8 |
| format | Article |
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L. plantarum
serves as the probiotic host strain, known for its beneficial properties in the gut microbiota.
E. coli
is used as a cloning host for the initial genetic engineering steps, facilitating the construction and amplification of the desired genetic constructs. In this study, using synthetic biology technology, we constructed engineered probiotics which could adhere and display nanobody on the surface to neutralize virus. Firstly, we screen an optimal nanobody to effectively bind with PRRSV by building library, expression and purification. Then, the integration of adhesion protein and nanobody into the genome of probiotics significantly improved its adhesion to IPEC-J2 cells. In addition, this engineered probiotic is almost non-toxic to cells with good safety, which can be used as a daily probiotics to prevent virus fecal transmission. Our study proposed this novel construction strategy of engineering probiotics with both adhesion and neutralization effects, which provided a new therapeutic view for intestinal virus clearance.
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L. plantarum
serves as the probiotic host strain, known for its beneficial properties in the gut microbiota.
E. coli
is used as a cloning host for the initial genetic engineering steps, facilitating the construction and amplification of the desired genetic constructs. In this study, using synthetic biology technology, we constructed engineered probiotics which could adhere and display nanobody on the surface to neutralize virus. Firstly, we screen an optimal nanobody to effectively bind with PRRSV by building library, expression and purification. Then, the integration of adhesion protein and nanobody into the genome of probiotics significantly improved its adhesion to IPEC-J2 cells. In addition, this engineered probiotic is almost non-toxic to cells with good safety, which can be used as a daily probiotics to prevent virus fecal transmission. Our study proposed this novel construction strategy of engineering probiotics with both adhesion and neutralization effects, which provided a new therapeutic view for intestinal virus clearance.
Graphical Abstract</description><subject>Adhesion</subject><subject>Animal diseases</subject><subject>Animals</subject><subject>Bacterial Adhesion</subject><subject>Biochemistry</subject><subject>Biological effects</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Breeding</subject><subject>Cell Biology</subject><subject>Cell Line</subject><subject>Cloning</subject><subject>Construction engineering</subject><subject>E coli</subject><subject>Ecology</subject><subject>Escherichia coli - genetics</subject><subject>Genetic Engineering</subject><subject>Host plants</subject><subject>Infectivity</subject><subject>Intestinal microflora</subject><subject>Lactobacillus plantarum - genetics</subject><subject>Lactobacillus plantarum - immunology</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Nanobodies</subject><subject>Neutralization</subject><subject>Original Paper</subject><subject>Plant breeding</subject><subject>Porcine Reproductive and Respiratory Syndrome - immunology</subject><subject>Porcine Reproductive and Respiratory Syndrome - prevention & control</subject><subject>Porcine Reproductive and Respiratory Syndrome - virology</subject><subject>Porcine respiratory and reproductive syndrome virus - genetics</subject><subject>Porcine respiratory and reproductive syndrome virus - immunology</subject><subject>Probiotics</subject><subject>Probiotics - pharmacology</subject><subject>Protein purification</subject><subject>Respiratory diseases</subject><subject>Single-Domain Antibodies - genetics</subject><subject>Single-Domain Antibodies - immunology</subject><subject>Swine</subject><subject>Viral diseases</subject><subject>Viruses</subject><issn>0302-8933</issn><issn>1432-072X</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1rFTEUhoMo9lr9Ay4k4MbNaD7vJEu5-FEouFFwFzLJmTZlbjImmcL0D_i3ze1UBReuAsnzPjmcF6GXlLylhPTvCiGM8I4w0RFBterUI7SjgrOO9Oz7Y7QjnLBOac7P0LNSbgihTCn1FJ1xLQXRe7lDPw8plpoXV0OKOI0Y4lWIABk8nnMaQqrB4XptK7b-ul1jGz32ocyTXXG0MQ3Jr7gmHGGp2U7hDvCcsmsSnKEp_Ml9u-UylDlkW1NecVmjz-kI-DbkpTxHT0Y7FXjxcJ6jbx8_fD187i6_fLo4vL_sHJP72o1sENwL7hx470ZuNdV6YDD2FLyWI4AalCVSCeFg1GAFU0xI6z2TkknBz9Gbzdsm-7FAqeYYioNpshHSUgw_rYixfk8b-vof9CYtObbp7inRK8F4o9hGuZxKyTCaOYejzauhxJxqMltNptVk7msyqoVePaiX4Qj-T-R3Lw3gG1DaU7yC_Pfv_2h_Ae1qobc</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Zhou, Li</creator><creator>Zhou, Hanlin</creator><creator>Wang, Panying</creator><creator>Xu, Hang</creator><creator>Wu, Jiayi</creator><creator>Zhou, Yuanzhuo</creator><creator>Feng, Jiaying</creator><creator>Zheng, Weiyi</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20241201</creationdate><title>Construction of engineered probiotic that adhere and display nanobody to neutralize porcine reproductive and respiratory syndrome virus</title><author>Zhou, Li ; Zhou, Hanlin ; Wang, Panying ; Xu, Hang ; Wu, Jiayi ; Zhou, Yuanzhuo ; Feng, Jiaying ; Zheng, Weiyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-f2b43d43cceddcf3a9199b2ef71ed95fee8b8a05844cef9ea428245add2552543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adhesion</topic><topic>Animal diseases</topic><topic>Animals</topic><topic>Bacterial Adhesion</topic><topic>Biochemistry</topic><topic>Biological effects</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Breeding</topic><topic>Cell Biology</topic><topic>Cell Line</topic><topic>Cloning</topic><topic>Construction engineering</topic><topic>E coli</topic><topic>Ecology</topic><topic>Escherichia coli - genetics</topic><topic>Genetic Engineering</topic><topic>Host plants</topic><topic>Infectivity</topic><topic>Intestinal microflora</topic><topic>Lactobacillus plantarum - genetics</topic><topic>Lactobacillus plantarum - immunology</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Nanobodies</topic><topic>Neutralization</topic><topic>Original Paper</topic><topic>Plant breeding</topic><topic>Porcine Reproductive and Respiratory Syndrome - immunology</topic><topic>Porcine Reproductive and Respiratory Syndrome - prevention & control</topic><topic>Porcine Reproductive and Respiratory Syndrome - virology</topic><topic>Porcine respiratory and reproductive syndrome virus - genetics</topic><topic>Porcine respiratory and reproductive syndrome virus - immunology</topic><topic>Probiotics</topic><topic>Probiotics - pharmacology</topic><topic>Protein purification</topic><topic>Respiratory diseases</topic><topic>Single-Domain Antibodies - genetics</topic><topic>Single-Domain Antibodies - immunology</topic><topic>Swine</topic><topic>Viral diseases</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Li</creatorcontrib><creatorcontrib>Zhou, Hanlin</creatorcontrib><creatorcontrib>Wang, Panying</creatorcontrib><creatorcontrib>Xu, Hang</creatorcontrib><creatorcontrib>Wu, Jiayi</creatorcontrib><creatorcontrib>Zhou, Yuanzhuo</creatorcontrib><creatorcontrib>Feng, Jiaying</creatorcontrib><creatorcontrib>Zheng, Weiyi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Li</au><au>Zhou, Hanlin</au><au>Wang, Panying</au><au>Xu, Hang</au><au>Wu, Jiayi</au><au>Zhou, Yuanzhuo</au><au>Feng, Jiaying</au><au>Zheng, Weiyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of engineered probiotic that adhere and display nanobody to neutralize porcine reproductive and respiratory syndrome virus</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>206</volume><issue>12</issue><spage>466</spage><pages>466-</pages><artnum>466</artnum><issn>0302-8933</issn><issn>1432-072X</issn><eissn>1432-072X</eissn><abstract>Pathogenic blue ear disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) bring severe loss to breeding industry due to high infectivity and mortality.
L. plantarum
serves as the probiotic host strain, known for its beneficial properties in the gut microbiota.
E. coli
is used as a cloning host for the initial genetic engineering steps, facilitating the construction and amplification of the desired genetic constructs. In this study, using synthetic biology technology, we constructed engineered probiotics which could adhere and display nanobody on the surface to neutralize virus. Firstly, we screen an optimal nanobody to effectively bind with PRRSV by building library, expression and purification. Then, the integration of adhesion protein and nanobody into the genome of probiotics significantly improved its adhesion to IPEC-J2 cells. In addition, this engineered probiotic is almost non-toxic to cells with good safety, which can be used as a daily probiotics to prevent virus fecal transmission. Our study proposed this novel construction strategy of engineering probiotics with both adhesion and neutralization effects, which provided a new therapeutic view for intestinal virus clearance.
Graphical Abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>39540965</pmid><doi>10.1007/s00203-024-04198-8</doi></addata></record> |
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| ispartof | Archives of microbiology, 2024-12, Vol.206 (12), p.466, Article 466 |
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| subjects | Adhesion Animal diseases Animals Bacterial Adhesion Biochemistry Biological effects Biomedical and Life Sciences Biotechnology Breeding Cell Biology Cell Line Cloning Construction engineering E coli Ecology Escherichia coli - genetics Genetic Engineering Host plants Infectivity Intestinal microflora Lactobacillus plantarum - genetics Lactobacillus plantarum - immunology Life Sciences Microbial Ecology Microbiology Nanobodies Neutralization Original Paper Plant breeding Porcine Reproductive and Respiratory Syndrome - immunology Porcine Reproductive and Respiratory Syndrome - prevention & control Porcine Reproductive and Respiratory Syndrome - virology Porcine respiratory and reproductive syndrome virus - genetics Porcine respiratory and reproductive syndrome virus - immunology Probiotics Probiotics - pharmacology Protein purification Respiratory diseases Single-Domain Antibodies - genetics Single-Domain Antibodies - immunology Swine Viral diseases Viruses |
| title | Construction of engineered probiotic that adhere and display nanobody to neutralize porcine reproductive and respiratory syndrome virus |
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