Highly sensitive detection of MMP-2 using an electrochemiluminescent biosensor enhanced by ladder-branch hybridization chain reaction

An advanced electrochemiluminescence (ECL) biosensor was developed that integrates T7 RNA polymerase amplification, ladder-branch hybridization chain reaction (HCR), and the precise targeting capabilities of CRISPR/Cas13a technology. The novelty of this research lies in the unique combination of the...

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Veröffentlicht in:Mikrochimica acta (1966) 2024-12, Vol.191 (12), p.742, Article 742
Hauptverfasser: Gong, Yuanxun, Zhang, Jiayi, Xi, Yunzhan, Jian, Dingxie, Zhuo, Chenyi, Tang, Qianli, Zhang, Kai, Liao, Xianjiu
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container_issue 12
container_start_page 742
container_title Mikrochimica acta (1966)
container_volume 191
creator Gong, Yuanxun
Zhang, Jiayi
Xi, Yunzhan
Jian, Dingxie
Zhuo, Chenyi
Tang, Qianli
Zhang, Kai
Liao, Xianjiu
description An advanced electrochemiluminescence (ECL) biosensor was developed that integrates T7 RNA polymerase amplification, ladder-branch hybridization chain reaction (HCR), and the precise targeting capabilities of CRISPR/Cas13a technology. The novelty of this research lies in the unique combination of these three cutting-edge technologies, which has not been previously utilized together in biosensing platforms, enabling highly sensitive and specific detection of biomolecules with exceptional precision. This innovative biosensor addresses the critical need for sensitive and specific detection of matrix metalloproteinase-2 (MMP-2), a key biomarker in cancer diagnostics. Through meticulous optimization of amplification and reaction conditions, the biosensor demonstrated remarkable sensitivity and specificity, achieving a detection limit as low as 6.34 aM, surpassing existing methodologies. The biosensor also exhibited excellent stability and reproducibility across multiple scans and maintained consistent functionality over an extended period, highlighting its reliability for practical applications. The effectiveness of the biosensor was validated using real samples, demonstrating its capability to accurately quantify MMP-2 in complex biological matrices with high recovery and minimal interference. The integration of isothermal amplification and CRISPR/Cas13a within the ECL biosensor platform represents a significant advancement in molecular diagnostics, offering a powerful tool for real-time monitoring of MMP-2. This combination of technologies sets the platform apart from traditional methods, marking a significant step forward in biosensor innovation. This biosensor holds substantial promise for revolutionizing cancer diagnostics and facilitating personalized treatment strategies, ultimately aiming to improve patient outcomes in cancer care. Future research may explore further enhancements and applications of this biosensor in various clinical settings. Graphical Abstract
doi_str_mv 10.1007/s00604-024-06761-y
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The effectiveness of the biosensor was validated using real samples, demonstrating its capability to accurately quantify MMP-2 in complex biological matrices with high recovery and minimal interference. The integration of isothermal amplification and CRISPR/Cas13a within the ECL biosensor platform represents a significant advancement in molecular diagnostics, offering a powerful tool for real-time monitoring of MMP-2. This combination of technologies sets the platform apart from traditional methods, marking a significant step forward in biosensor innovation. This biosensor holds substantial promise for revolutionizing cancer diagnostics and facilitating personalized treatment strategies, ultimately aiming to improve patient outcomes in cancer care. Future research may explore further enhancements and applications of this biosensor in various clinical settings. 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source MEDLINE; Springer journals
subjects Analytical Chemistry
Biological effects
Biomarkers
Biomolecules
Biosensing Techniques - methods
Biosensors
Cancer
Chain branching
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
CRISPR
CRISPR-Cas Systems
Electrochemical Techniques - methods
Electrochemiluminescence
Humans
Limit of Detection
Luminescent Measurements - methods
Matrix Metalloproteinase 2 - genetics
Matrix metalloproteinases
Microengineering
Nanochemistry
Nanotechnology
Nucleic Acid Amplification Techniques - methods
Nucleic Acid Hybridization
Real time
RNA polymerase
title Highly sensitive detection of MMP-2 using an electrochemiluminescent biosensor enhanced by ladder-branch hybridization chain reaction
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