Highly sensitive detection of MMP-2 using an electrochemiluminescent biosensor enhanced by ladder-branch hybridization chain reaction
An advanced electrochemiluminescence (ECL) biosensor was developed that integrates T7 RNA polymerase amplification, ladder-branch hybridization chain reaction (HCR), and the precise targeting capabilities of CRISPR/Cas13a technology. The novelty of this research lies in the unique combination of the...
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Veröffentlicht in: | Mikrochimica acta (1966) 2024-12, Vol.191 (12), p.742, Article 742 |
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creator | Gong, Yuanxun Zhang, Jiayi Xi, Yunzhan Jian, Dingxie Zhuo, Chenyi Tang, Qianli Zhang, Kai Liao, Xianjiu |
description | An advanced electrochemiluminescence (ECL) biosensor was developed that integrates T7 RNA polymerase amplification, ladder-branch hybridization chain reaction (HCR), and the precise targeting capabilities of CRISPR/Cas13a technology. The novelty of this research lies in the unique combination of these three cutting-edge technologies, which has not been previously utilized together in biosensing platforms, enabling highly sensitive and specific detection of biomolecules with exceptional precision. This innovative biosensor addresses the critical need for sensitive and specific detection of matrix metalloproteinase-2 (MMP-2), a key biomarker in cancer diagnostics. Through meticulous optimization of amplification and reaction conditions, the biosensor demonstrated remarkable sensitivity and specificity, achieving a detection limit as low as 6.34 aM, surpassing existing methodologies. The biosensor also exhibited excellent stability and reproducibility across multiple scans and maintained consistent functionality over an extended period, highlighting its reliability for practical applications. The effectiveness of the biosensor was validated using real samples, demonstrating its capability to accurately quantify MMP-2 in complex biological matrices with high recovery and minimal interference. The integration of isothermal amplification and CRISPR/Cas13a within the ECL biosensor platform represents a significant advancement in molecular diagnostics, offering a powerful tool for real-time monitoring of MMP-2. This combination of technologies sets the platform apart from traditional methods, marking a significant step forward in biosensor innovation. This biosensor holds substantial promise for revolutionizing cancer diagnostics and facilitating personalized treatment strategies, ultimately aiming to improve patient outcomes in cancer care. Future research may explore further enhancements and applications of this biosensor in various clinical settings.
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doi_str_mv | 10.1007/s00604-024-06761-y |
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The novelty of this research lies in the unique combination of these three cutting-edge technologies, which has not been previously utilized together in biosensing platforms, enabling highly sensitive and specific detection of biomolecules with exceptional precision. This innovative biosensor addresses the critical need for sensitive and specific detection of matrix metalloproteinase-2 (MMP-2), a key biomarker in cancer diagnostics. Through meticulous optimization of amplification and reaction conditions, the biosensor demonstrated remarkable sensitivity and specificity, achieving a detection limit as low as 6.34 aM, surpassing existing methodologies. The biosensor also exhibited excellent stability and reproducibility across multiple scans and maintained consistent functionality over an extended period, highlighting its reliability for practical applications. The effectiveness of the biosensor was validated using real samples, demonstrating its capability to accurately quantify MMP-2 in complex biological matrices with high recovery and minimal interference. The integration of isothermal amplification and CRISPR/Cas13a within the ECL biosensor platform represents a significant advancement in molecular diagnostics, offering a powerful tool for real-time monitoring of MMP-2. This combination of technologies sets the platform apart from traditional methods, marking a significant step forward in biosensor innovation. This biosensor holds substantial promise for revolutionizing cancer diagnostics and facilitating personalized treatment strategies, ultimately aiming to improve patient outcomes in cancer care. Future research may explore further enhancements and applications of this biosensor in various clinical settings.
Graphical Abstract</description><subject>Analytical Chemistry</subject><subject>Biological effects</subject><subject>Biomarkers</subject><subject>Biomolecules</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Cancer</subject><subject>Chain branching</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrochemiluminescence</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Luminescent Measurements - methods</subject><subject>Matrix Metalloproteinase 2 - genetics</subject><subject>Matrix metalloproteinases</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Nucleic Acid Hybridization</subject><subject>Real time</subject><subject>RNA polymerase</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS0EotvCF-CALHHhYuo_ie0cUQUtUqtyaM-W7cxuXCV2sROkcOd7490tIHHgYI3s-c17Hj2E3jD6gVGqzgulkjaE8nqkkoysz9CGNUKSlirxHG0o5ZIIqfgJOi3lgVKmJG9eohPRtaKVrNugn1dhN4wrLhBLmMN3wD3M4OeQIk5bfHPzlXC8lBB32EYMY23l5AeYwrhMIULxEGfsQtoLpIwhDjZ66LFb8Wj7HjJxub4MeFhdDn34YQ_afrAh4gz2YPUKvdjascDrp3qG7j9_uru4Ite3l18uPl4Tz1s5Ey8UKNHxXjghO-0Y85pL3VJhpfe-AZAtdV5tVafaVljHLdOsp1zUq9ZanKH3R93HnL4tUGYzhbrBONoIaSlGMK41Uw3rKvruH_QhLTnW3x0oXl2bPcWPlM-plAxb85jDZPNqGDX7kMwxJFNDMoeQzFqH3j5JL26C_s_I71QqII5Aqa24g_zX-z-yvwDwjJ57</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Gong, Yuanxun</creator><creator>Zhang, Jiayi</creator><creator>Xi, Yunzhan</creator><creator>Jian, Dingxie</creator><creator>Zhuo, Chenyi</creator><creator>Tang, Qianli</creator><creator>Zhang, Kai</creator><creator>Liao, Xianjiu</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20241201</creationdate><title>Highly sensitive detection of MMP-2 using an electrochemiluminescent biosensor enhanced by ladder-branch hybridization chain reaction</title><author>Gong, Yuanxun ; 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The novelty of this research lies in the unique combination of these three cutting-edge technologies, which has not been previously utilized together in biosensing platforms, enabling highly sensitive and specific detection of biomolecules with exceptional precision. This innovative biosensor addresses the critical need for sensitive and specific detection of matrix metalloproteinase-2 (MMP-2), a key biomarker in cancer diagnostics. Through meticulous optimization of amplification and reaction conditions, the biosensor demonstrated remarkable sensitivity and specificity, achieving a detection limit as low as 6.34 aM, surpassing existing methodologies. The biosensor also exhibited excellent stability and reproducibility across multiple scans and maintained consistent functionality over an extended period, highlighting its reliability for practical applications. The effectiveness of the biosensor was validated using real samples, demonstrating its capability to accurately quantify MMP-2 in complex biological matrices with high recovery and minimal interference. The integration of isothermal amplification and CRISPR/Cas13a within the ECL biosensor platform represents a significant advancement in molecular diagnostics, offering a powerful tool for real-time monitoring of MMP-2. This combination of technologies sets the platform apart from traditional methods, marking a significant step forward in biosensor innovation. This biosensor holds substantial promise for revolutionizing cancer diagnostics and facilitating personalized treatment strategies, ultimately aiming to improve patient outcomes in cancer care. Future research may explore further enhancements and applications of this biosensor in various clinical settings.
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subjects | Analytical Chemistry Biological effects Biomarkers Biomolecules Biosensing Techniques - methods Biosensors Cancer Chain branching Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science CRISPR CRISPR-Cas Systems Electrochemical Techniques - methods Electrochemiluminescence Humans Limit of Detection Luminescent Measurements - methods Matrix Metalloproteinase 2 - genetics Matrix metalloproteinases Microengineering Nanochemistry Nanotechnology Nucleic Acid Amplification Techniques - methods Nucleic Acid Hybridization Real time RNA polymerase |
title | Highly sensitive detection of MMP-2 using an electrochemiluminescent biosensor enhanced by ladder-branch hybridization chain reaction |
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