Insights into the photoswitch based on 5-deazaFMN and LOV2 from Avena sativa : a combined absorption and NMR spectroscopy study
The LOV2 domain from ( ) is a blue light receptor that undergoes adduct formation with the native flavin mononucleotide (FMN) cofactor [Salomon , , 2000, , 9401]. We report the photochemical changes of LOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows tha...
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| Veröffentlicht in: | Physical chemistry chemical physics : PCCP 2024-11, Vol.26 (46), p.28884-28893 |
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| container_title | Physical chemistry chemical physics : PCCP |
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| creator | Panter, Sabrina Wörner, Jakob Chen, Jing Illarionov, Boris Bacher, Adelbert Fischer, Markus Weber, Stefan |
| description | The LOV2 domain from
(
) is a blue light receptor that undergoes adduct formation with the native flavin mononucleotide (FMN) cofactor [Salomon
,
, 2000,
, 9401]. We report the photochemical changes of
LOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed. We were able to confirm the structure of the adduct by introducing
C-labelled 5-deazaFMN isotopologues in solution NMR experiments. Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss. Based on the data presented we propose the system as an alternative to wild-type
LOV2 for applications in optogenetics. |
| doi_str_mv | 10.1039/d4cp02714k |
| format | Article |
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(
) is a blue light receptor that undergoes adduct formation with the native flavin mononucleotide (FMN) cofactor [Salomon
,
, 2000,
, 9401]. We report the photochemical changes of
LOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed. We were able to confirm the structure of the adduct by introducing
C-labelled 5-deazaFMN isotopologues in solution NMR experiments. Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss. Based on the data presented we propose the system as an alternative to wild-type
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(
) is a blue light receptor that undergoes adduct formation with the native flavin mononucleotide (FMN) cofactor [Salomon
,
, 2000,
, 9401]. We report the photochemical changes of
LOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed. We were able to confirm the structure of the adduct by introducing
C-labelled 5-deazaFMN isotopologues in solution NMR experiments. Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss. Based on the data presented we propose the system as an alternative to wild-type
LOV2 for applications in optogenetics.</description><subject>Absorption spectroscopy</subject><subject>Dark adaptation</subject><subject>NMR spectroscopy</subject><issn>1463-9076</issn><issn>1463-9084</issn><issn>1463-9084</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpdkUlLxEAQhRtR3C_-AGnwIkK0t2zeZFxxXBD1GirdFSc6Scd0Rxkv_nXb9eCheHX43qOKR8gGZ7ucyXzPKN0xkXL1NEeWuUpklLNMzf_tabJEVpx7ZIzxmMtFsiTzWIbJlsn7Wevqh4l3tG69pX6CtJtYb91r7fWEluDQUNvSODIIb3B8cUmhNXR8dS9o1duGHrxgC9SBr1-A7lOg2jZl3QYXlM72na-D-9NyeXFDXYfa99Zp282o84OZrZGFCqYO1390ldwdH92OTqPx1cnZ6GAcaaGYj2SFRioGVZlzo4zRMcYJpJU2IACQlUxhplgCPEisRI6IJk44ihSYEJVcJdvfuV1vnwd0vmhqp3E6hRbt4ArJRZbxhCdZQLf-oY926NtwXaCkFEmu4jRQO9-UDv-4Hqui6-sG-lnBWfFZS3GoRtdftZwHePMncigbNH_obw_yA0kmiDs</recordid><startdate>20241127</startdate><enddate>20241127</enddate><creator>Panter, Sabrina</creator><creator>Wörner, Jakob</creator><creator>Chen, Jing</creator><creator>Illarionov, Boris</creator><creator>Bacher, Adelbert</creator><creator>Fischer, Markus</creator><creator>Weber, Stefan</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0001-0602-8579</orcidid><orcidid>https://orcid.org/0009-0007-8489-0651</orcidid><orcidid>https://orcid.org/0000-0003-4090-7435</orcidid><orcidid>https://orcid.org/0000-0001-7243-4199</orcidid><orcidid>https://orcid.org/0000-0001-5174-4139</orcidid></search><sort><creationdate>20241127</creationdate><title>Insights into the photoswitch based on 5-deazaFMN and LOV2 from Avena sativa : a combined absorption and NMR spectroscopy study</title><author>Panter, Sabrina ; Wörner, Jakob ; Chen, Jing ; Illarionov, Boris ; Bacher, Adelbert ; Fischer, Markus ; Weber, Stefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c240t-3fed340afb91d4ddc5e56a7fcda2aae0b04e8406a1e845429eeed561e27a022f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Absorption spectroscopy</topic><topic>Dark adaptation</topic><topic>NMR spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panter, Sabrina</creatorcontrib><creatorcontrib>Wörner, Jakob</creatorcontrib><creatorcontrib>Chen, Jing</creatorcontrib><creatorcontrib>Illarionov, Boris</creatorcontrib><creatorcontrib>Bacher, Adelbert</creatorcontrib><creatorcontrib>Fischer, Markus</creatorcontrib><creatorcontrib>Weber, Stefan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Physical chemistry chemical physics : PCCP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panter, Sabrina</au><au>Wörner, Jakob</au><au>Chen, Jing</au><au>Illarionov, Boris</au><au>Bacher, Adelbert</au><au>Fischer, Markus</au><au>Weber, Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insights into the photoswitch based on 5-deazaFMN and LOV2 from Avena sativa : a combined absorption and NMR spectroscopy study</atitle><jtitle>Physical chemistry chemical physics : PCCP</jtitle><addtitle>Phys Chem Chem Phys</addtitle><date>2024-11-27</date><risdate>2024</risdate><volume>26</volume><issue>46</issue><spage>28884</spage><epage>28893</epage><pages>28884-28893</pages><issn>1463-9076</issn><issn>1463-9084</issn><eissn>1463-9084</eissn><abstract>The LOV2 domain from
(
) is a blue light receptor that undergoes adduct formation with the native flavin mononucleotide (FMN) cofactor [Salomon
,
, 2000,
, 9401]. We report the photochemical changes of
LOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed. We were able to confirm the structure of the adduct by introducing
C-labelled 5-deazaFMN isotopologues in solution NMR experiments. Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss. Based on the data presented we propose the system as an alternative to wild-type
LOV2 for applications in optogenetics.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>39533958</pmid><doi>10.1039/d4cp02714k</doi><tpages>10</tpages><orcidid>https://orcid.org/0009-0001-0602-8579</orcidid><orcidid>https://orcid.org/0009-0007-8489-0651</orcidid><orcidid>https://orcid.org/0000-0003-4090-7435</orcidid><orcidid>https://orcid.org/0000-0001-7243-4199</orcidid><orcidid>https://orcid.org/0000-0001-5174-4139</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Absorption spectroscopy Dark adaptation NMR spectroscopy |
| title | Insights into the photoswitch based on 5-deazaFMN and LOV2 from Avena sativa : a combined absorption and NMR spectroscopy study |
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