Characterization and synergistic activity of heterologously expressed microbial-derived endoglucanase and bifunctional cellulase on wheat straw

Cellulases are divided into endoglucanase, exoglucanase, and β-glucosidase based on their catalytic activity. Eight cellulases were recombinantly expressed in Escherichia coli BL21 to investigate their effects on the enzymatic hydrolysis of wheat straw. Among them, cellulase 2006 exhibited the highe...

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Veröffentlicht in:	International journal of biological macromolecules 2024-12, Vol.282 (Pt 6), p.137485, Article 137485
Hauptverfasser:	Wang, Zhiwei, Tang, Haoran, Li, Yangguang, Yang, Bohua, Liang, Xuhui, Gong, Hanxuan, Chen, Yulin, Liu, Gongwei, Yang, Yuxin
Format:	Artikel
Sprache:	eng
Schlagworte:	Cellulase Cellulase - chemistry Cellulase - genetics Cellulase - metabolism Cellulose - chemistry Cellulose - metabolism Enzymatic characterization Enzymatic hydrolysis Enzyme Stability Escherichia coli - genetics Gene Expression Hydrogen-Ion Concentration Hydrolysis Kinetics Prokaryotic expression Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Substrate Specificity Temperature Triticum Wheat straw
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