A Dual Column pH Switchable Water Stationary Phase System for Separation Control in Supercritical Fluid Chromatography
ABSTRACT A dual column system comprised of a pH switchable water stationary phase column and a conventional non‐polar capillary column is introduced for use in Supercritical Fluid Chromatography (SFC). By removing or adding NH4OH to the system hydration source, the water stationary phase pH can be r...
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| Veröffentlicht in: | Journal of separation science 2024-11, Vol.47 (21), p.e70008-n/a |
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| container_title | Journal of separation science |
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| creator | Nai, Emmanuel A. Thurbide, Kevin B. |
| description | ABSTRACT
A dual column system comprised of a pH switchable water stationary phase column and a conventional non‐polar capillary column is introduced for use in Supercritical Fluid Chromatography (SFC). By removing or adding NH4OH to the system hydration source, the water stationary phase pH can be rapidly switched between acidic (measured at pH∼3) and basic (measured at pH∼9) in seconds, while the operating character of the conventional column is unchanged. This switch modulates the velocity of ionizable analytes about 20‐fold in the system, whereas non‐ionizable analytes are not affected. In this way, the retention time of acids and/or bases can be reproducibly altered ( |
| doi_str_mv | 10.1002/jssc.70008 |
| format | Article |
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A dual column system comprised of a pH switchable water stationary phase column and a conventional non‐polar capillary column is introduced for use in Supercritical Fluid Chromatography (SFC). By removing or adding NH4OH to the system hydration source, the water stationary phase pH can be rapidly switched between acidic (measured at pH∼3) and basic (measured at pH∼9) in seconds, while the operating character of the conventional column is unchanged. This switch modulates the velocity of ionizable analytes about 20‐fold in the system, whereas non‐ionizable analytes are not affected. In this way, the retention time of acids and/or bases can be reproducibly altered (<1% RSD; n = 3) in SFC separations. As a result, analyte selectivity and resolution can be readily controlled during analyses. For example, a selectivity reversal (alpha from 0.4 to 1.6) and a resolution increase (from 0 to 13) are demonstrated. Rapid stationary phase pH switching also allows multiple acids, bases, and/or neutral analytes to be determined simultaneously. Applications demonstrate that this method can greatly simplify complex mixture analysis in SFC by helping to separate target analytes from interfering matrix components.</description><identifier>ISSN: 1615-9306</identifier><identifier>ISSN: 1615-9314</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.70008</identifier><identifier>PMID: 39494766</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Ammonium hydroxide ; chemical species ; Chromatography ; Column chromatography ; dual column ; separation ; supercritical fluid chromatography ; Supercritical fluids ; switchable stationary phase ; water</subject><ispartof>Journal of separation science, 2024-11, Vol.47 (21), p.e70008-n/a</ispartof><rights>2024 Wiley‐VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3338-f7f3257a18e44d943afb2ccf788d3698567252a0400390ccc04b0bd5930f6253</cites><orcidid>0000-0001-7065-5801</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.70008$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.70008$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39494766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nai, Emmanuel A.</creatorcontrib><creatorcontrib>Thurbide, Kevin B.</creatorcontrib><title>A Dual Column pH Switchable Water Stationary Phase System for Separation Control in Supercritical Fluid Chromatography</title><title>Journal of separation science</title><addtitle>J Sep Sci</addtitle><description>ABSTRACT
A dual column system comprised of a pH switchable water stationary phase column and a conventional non‐polar capillary column is introduced for use in Supercritical Fluid Chromatography (SFC). By removing or adding NH4OH to the system hydration source, the water stationary phase pH can be rapidly switched between acidic (measured at pH∼3) and basic (measured at pH∼9) in seconds, while the operating character of the conventional column is unchanged. This switch modulates the velocity of ionizable analytes about 20‐fold in the system, whereas non‐ionizable analytes are not affected. In this way, the retention time of acids and/or bases can be reproducibly altered (<1% RSD; n = 3) in SFC separations. As a result, analyte selectivity and resolution can be readily controlled during analyses. For example, a selectivity reversal (alpha from 0.4 to 1.6) and a resolution increase (from 0 to 13) are demonstrated. Rapid stationary phase pH switching also allows multiple acids, bases, and/or neutral analytes to be determined simultaneously. Applications demonstrate that this method can greatly simplify complex mixture analysis in SFC by helping to separate target analytes from interfering matrix components.</description><subject>Ammonium hydroxide</subject><subject>chemical species</subject><subject>Chromatography</subject><subject>Column chromatography</subject><subject>dual column</subject><subject>separation</subject><subject>supercritical fluid chromatography</subject><subject>Supercritical fluids</subject><subject>switchable stationary phase</subject><subject>water</subject><issn>1615-9306</issn><issn>1615-9314</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNkc9L5TAQx4O4qOvuxT9AAl5k4emkSdr0KPXnIqxQYY8lTVNfHmlTk1R5_715PtfDHpY9zcB8-AwzX4SOCJwRgOx8FYI6KwBA7KADkhO-KClhu5895PvoawgrAFKIEvbQPi1ZyYo8P0AvF_hylhZXzs7DiKdbXL-aqJaytRr_llF7XEcZjRulX-OHpQwa1-sQ9YB7l2Z6kv59nAxj9M5iM-J6nrRX3kSjkvrazqbD1dK7QUb35OW0XH9DX3ppg_7-UQ_R4_XVY3W7uP91c1dd3C8UpVQs-qKnGS8kEZqxrmRU9m2mVF8I0dG8FDwvMp5JYAC0BKUUsBbajqeb-zzj9BCdbrWTd8-zDrEZTFDaWjlqN4eGEs4IL0Gw_0AzKqAAShJ68he6crMf0x0bSgDNOWx2_9hSyrsQvO6byZshfbEh0Gxyaza5Ne-5Jfj4Qzm3g-4-0T9BJYBsgVdj9fofquZnXVdb6Rt61qHP</recordid><startdate>202411</startdate><enddate>202411</enddate><creator>Nai, Emmanuel A.</creator><creator>Thurbide, Kevin B.</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-7065-5801</orcidid></search><sort><creationdate>202411</creationdate><title>A Dual Column pH Switchable Water Stationary Phase System for Separation Control in Supercritical Fluid Chromatography</title><author>Nai, Emmanuel A. ; Thurbide, Kevin B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3338-f7f3257a18e44d943afb2ccf788d3698567252a0400390ccc04b0bd5930f6253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Ammonium hydroxide</topic><topic>chemical species</topic><topic>Chromatography</topic><topic>Column chromatography</topic><topic>dual column</topic><topic>separation</topic><topic>supercritical fluid chromatography</topic><topic>Supercritical fluids</topic><topic>switchable stationary phase</topic><topic>water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nai, Emmanuel A.</creatorcontrib><creatorcontrib>Thurbide, Kevin B.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nai, Emmanuel A.</au><au>Thurbide, Kevin B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Dual Column pH Switchable Water Stationary Phase System for Separation Control in Supercritical Fluid Chromatography</atitle><jtitle>Journal of separation science</jtitle><addtitle>J Sep Sci</addtitle><date>2024-11</date><risdate>2024</risdate><volume>47</volume><issue>21</issue><spage>e70008</spage><epage>n/a</epage><pages>e70008-n/a</pages><issn>1615-9306</issn><issn>1615-9314</issn><eissn>1615-9314</eissn><abstract>ABSTRACT
A dual column system comprised of a pH switchable water stationary phase column and a conventional non‐polar capillary column is introduced for use in Supercritical Fluid Chromatography (SFC). By removing or adding NH4OH to the system hydration source, the water stationary phase pH can be rapidly switched between acidic (measured at pH∼3) and basic (measured at pH∼9) in seconds, while the operating character of the conventional column is unchanged. This switch modulates the velocity of ionizable analytes about 20‐fold in the system, whereas non‐ionizable analytes are not affected. In this way, the retention time of acids and/or bases can be reproducibly altered (<1% RSD; n = 3) in SFC separations. As a result, analyte selectivity and resolution can be readily controlled during analyses. For example, a selectivity reversal (alpha from 0.4 to 1.6) and a resolution increase (from 0 to 13) are demonstrated. Rapid stationary phase pH switching also allows multiple acids, bases, and/or neutral analytes to be determined simultaneously. Applications demonstrate that this method can greatly simplify complex mixture analysis in SFC by helping to separate target analytes from interfering matrix components.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>39494766</pmid><doi>10.1002/jssc.70008</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7065-5801</orcidid></addata></record> |
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| ispartof | Journal of separation science, 2024-11, Vol.47 (21), p.e70008-n/a |
| issn | 1615-9306 1615-9314 1615-9314 |
| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Ammonium hydroxide chemical species Chromatography Column chromatography dual column separation supercritical fluid chromatography Supercritical fluids switchable stationary phase water |
| title | A Dual Column pH Switchable Water Stationary Phase System for Separation Control in Supercritical Fluid Chromatography |
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