Nurse honey bees filter fungicide residues to maintain larval health

Residues of plant protection products (PPPs) are frequently detected in bee matrices1,2,3,4,5,6 due to foraging bees collecting contaminated nectar and pollen, which they bring back to their hive. The collected material is further used by nurse bees to produce glandular secretions for feeding their larvae.7 Potential exposure to PPPs occurs through direct oral ingestion, contact during foraging, or interaction with contaminated hive material.8,9 Contaminants can pose health risks to adult worker bees,10,11 queens,12,13 drones (males),14 or larvae,15,16 potentially impacting colony health and productivity. However, residue concentrations can vary significantly between analyzed matrices, and potential accumulation or dilution steps have not been widely investigated. Although research has provided valuable insights into contamination risks, there remain gaps in our understanding of the entire pathway from field, via foragers, stored products, nurse bees, and finally to food jelly, i.e., royal, worker, and drone jelly, and the larvae, including all possible processing steps.17 We collected samples of bee-relevant matrices following the in-field spray application of the product Pictor Active, containing the fungicides boscalid and pyraclostrobin. The samples were analyzed for residues along this entire pathway. Fungicide residues were reduced by a factor of 8–80 from stored product to nurse bees’ heads, suggesting a filtering function of nurse bees. Furthermore, detected residues in larval food jelly resulted from added pollen and not from nurse bee secretions. Calculated risk quotients were at least twice as low as the threshold values, suggesting a low risk to honey bee colonies from these fungicides at the tested application rate. [Display omitted] •Residues were analyzed along the entire transfer pathway from field to larvae•Residue concentrations decrease along the transfer pathway•Risk quotients for adult bees and larvae were below threshold values•Nurse honey bees act as a filter in the transfer of contaminants to larvae Wueppenhorst et al. investigate the full transfer pathway of two fungicides, from in-field application to honey bee larval food jelly and the larvae. Residues were diluted along the pathway and, based on residue levels, low risks are suggested for adult bees and larvae, indicating a filtering function of the honey bee colony.