Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows ( Bos indicus )
The study was conducted on indigenous Tharparkar cow ( ) to evaluate FSH stimulation on follicular attributes, oocyte recovery and morpho-molecular developmental competence parameters concerning oocyte quality. A total of 20 OPU sessions were performed, which included 10 sessions in each FSH stimula...
Gespeichert in:
| Veröffentlicht in: | Zygote (Cambridge) 2024-10, Vol.32 (5), p.376-385 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 385 |
|---|---|
| container_issue | 5 |
| container_start_page | 376 |
| container_title | Zygote (Cambridge) |
| container_volume | 32 |
| creator | Donadkar, Manoj Kumar, Brijesh Singh, Sanjay Kumar Chandra, Pradeep Dangi, Pradeep Gawai, Mohan Jackson, Amala Jasrotia, Nancy Sharma, Shweta Chouhan, Vikrant Patra, M K Khan, Meraj Haider |
| description | The study was conducted on indigenous Tharparkar cow (
) to evaluate FSH stimulation on follicular attributes, oocyte recovery and morpho-molecular developmental competence parameters concerning oocyte quality. A total of 20 OPU sessions were performed, which included 10 sessions in each FSH stimulated at the dose of 130 µg divided into four sub-doses and non-stimulated. Findings on the size of follicles having ≥6 mm showed a significantly higher, however an opposite trend was observed in the case of smaller sized follicle ( |
| doi_str_mv | 10.1017/S0967199424000376 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3121591649</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3121591649</sourcerecordid><originalsourceid>FETCH-LOGICAL-c226t-399fa40302e83d8a33036922344e00360e4251c02d99214cd9eff426afb1273b3</originalsourceid><addsrcrecordid>eNplkctOHDEQRa0oKAwkH5BN5CVZNPGL7vGSoPCQiFhA1i2Pu8w4tLsaPwbxSfwlHvHYsCqV6p57pbqEfOfskDPe_bpmuu241kooxpjs2k9kwVWrm2Un2Wey2J6b7X2X7KX0v2q6TqsvZFdq1dVNLMjTX4zzGpuAI9gymkhhY8ZisseJOox0gA2MOAeYshmpxTBDhskCRUcR7WOGRCPk6KtuoHkdsdyuaY5mShtz66cK4aYEOnt715SZuoiBnl6fNyn7UANzpW7WJs4m3tX0AacaavEh0QP6GxP10-BtSfTnV7LjzJjg2-vcJ_9O_9ycnDeXV2cXJ8eXjRWizY3U2hnFJBOwlMPSSMlkq4WQSkH9UctAiSNumRi0FlzZQYNzSrTGrbjo5Eruk4MX3znifYGU--CThXE0E2BJveSCH2neKl2l_EVqI6YUwfVz9MHEx56zfttQ_6Ghyvx4tS-rAMM78VaJfAbnh45G</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3121591649</pqid></control><display><type>article</type><title>Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows ( Bos indicus )</title><source>MEDLINE</source><source>Cambridge University Press Journals Complete</source><creator>Donadkar, Manoj ; Kumar, Brijesh ; Singh, Sanjay Kumar ; Chandra, Pradeep ; Dangi, Pradeep ; Gawai, Mohan ; Jackson, Amala ; Jasrotia, Nancy ; Sharma, Shweta ; Chouhan, Vikrant ; Patra, M K ; Khan, Meraj Haider</creator><creatorcontrib>Donadkar, Manoj ; Kumar, Brijesh ; Singh, Sanjay Kumar ; Chandra, Pradeep ; Dangi, Pradeep ; Gawai, Mohan ; Jackson, Amala ; Jasrotia, Nancy ; Sharma, Shweta ; Chouhan, Vikrant ; Patra, M K ; Khan, Meraj Haider</creatorcontrib><description>The study was conducted on indigenous Tharparkar cow (
) to evaluate FSH stimulation on follicular attributes, oocyte recovery and morpho-molecular developmental competence parameters concerning oocyte quality. A total of 20 OPU sessions were performed, which included 10 sessions in each FSH stimulated at the dose of 130 µg divided into four sub-doses and non-stimulated. Findings on the size of follicles having ≥6 mm showed a significantly higher, however an opposite trend was observed in the case of smaller sized follicle (<6 mm) between stimulated and non-stimulated respectively. The stimulated cows had a significantly higher number as well as the percentage of oocytes of Grade A, having a diameter ≥120 µm and BCB
as compared to the non-stimulated cows. The relative mRNA expression profile of GDF9, BMP15, PCNA and BCL-2 genes was higher and BAX was lower in the FSH-stimulated cow. These results indicated that FSH stimulation before OPU in
cows has a significant impact on follicle size, oocyte yield, recovery, and their quality with respect to COC's, diameter and BCB
oocytes. Further, a significant increase in the relative mRNA expression levels of GDF9, BMP15 and PCNA genes in the FSH-stimulated group suggests that FSH plays a key role in modulating the expression of these important candidate genes and thus influencing oocyte quality. The higher mRNA expression of BCL-2 genes and concomitantly lower expression of BAX gene in FSH Stimulated cows indicates the protective role of these genes and preventing programmed cell death and thus promoting cell survival, quality and embryo development.</description><identifier>ISSN: 0967-1994</identifier><identifier>ISSN: 1469-8730</identifier><identifier>EISSN: 1469-8730</identifier><identifier>DOI: 10.1017/S0967199424000376</identifier><identifier>PMID: 39470002</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Bone Morphogenetic Protein 15 - genetics ; Bone Morphogenetic Protein 15 - pharmacology ; Cattle ; Female ; Fertilization in Vitro - methods ; Fertilization in Vitro - veterinary ; Follicle Stimulating Hormone - pharmacology ; Growth Differentiation Factor 9 - genetics ; Growth Differentiation Factor 9 - pharmacology ; Oocyte Retrieval - methods ; Oocyte Retrieval - veterinary ; Oocytes - cytology ; Oocytes - drug effects ; Oocytes - physiology ; Ovarian Follicle - drug effects ; Proliferating Cell Nuclear Antigen - genetics ; Proliferating Cell Nuclear Antigen - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism</subject><ispartof>Zygote (Cambridge), 2024-10, Vol.32 (5), p.376-385</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c226t-399fa40302e83d8a33036922344e00360e4251c02d99214cd9eff426afb1273b3</cites><orcidid>0000-0001-6153-0791</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39470002$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Donadkar, Manoj</creatorcontrib><creatorcontrib>Kumar, Brijesh</creatorcontrib><creatorcontrib>Singh, Sanjay Kumar</creatorcontrib><creatorcontrib>Chandra, Pradeep</creatorcontrib><creatorcontrib>Dangi, Pradeep</creatorcontrib><creatorcontrib>Gawai, Mohan</creatorcontrib><creatorcontrib>Jackson, Amala</creatorcontrib><creatorcontrib>Jasrotia, Nancy</creatorcontrib><creatorcontrib>Sharma, Shweta</creatorcontrib><creatorcontrib>Chouhan, Vikrant</creatorcontrib><creatorcontrib>Patra, M K</creatorcontrib><creatorcontrib>Khan, Meraj Haider</creatorcontrib><title>Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows ( Bos indicus )</title><title>Zygote (Cambridge)</title><addtitle>Zygote</addtitle><description>The study was conducted on indigenous Tharparkar cow (
) to evaluate FSH stimulation on follicular attributes, oocyte recovery and morpho-molecular developmental competence parameters concerning oocyte quality. A total of 20 OPU sessions were performed, which included 10 sessions in each FSH stimulated at the dose of 130 µg divided into four sub-doses and non-stimulated. Findings on the size of follicles having ≥6 mm showed a significantly higher, however an opposite trend was observed in the case of smaller sized follicle (<6 mm) between stimulated and non-stimulated respectively. The stimulated cows had a significantly higher number as well as the percentage of oocytes of Grade A, having a diameter ≥120 µm and BCB
as compared to the non-stimulated cows. The relative mRNA expression profile of GDF9, BMP15, PCNA and BCL-2 genes was higher and BAX was lower in the FSH-stimulated cow. These results indicated that FSH stimulation before OPU in
cows has a significant impact on follicle size, oocyte yield, recovery, and their quality with respect to COC's, diameter and BCB
oocytes. Further, a significant increase in the relative mRNA expression levels of GDF9, BMP15 and PCNA genes in the FSH-stimulated group suggests that FSH plays a key role in modulating the expression of these important candidate genes and thus influencing oocyte quality. The higher mRNA expression of BCL-2 genes and concomitantly lower expression of BAX gene in FSH Stimulated cows indicates the protective role of these genes and preventing programmed cell death and thus promoting cell survival, quality and embryo development.</description><subject>Animals</subject><subject>Bone Morphogenetic Protein 15 - genetics</subject><subject>Bone Morphogenetic Protein 15 - pharmacology</subject><subject>Cattle</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>Fertilization in Vitro - veterinary</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>Growth Differentiation Factor 9 - genetics</subject><subject>Growth Differentiation Factor 9 - pharmacology</subject><subject>Oocyte Retrieval - methods</subject><subject>Oocyte Retrieval - veterinary</subject><subject>Oocytes - cytology</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - physiology</subject><subject>Ovarian Follicle - drug effects</subject><subject>Proliferating Cell Nuclear Antigen - genetics</subject><subject>Proliferating Cell Nuclear Antigen - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0967-1994</issn><issn>1469-8730</issn><issn>1469-8730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkctOHDEQRa0oKAwkH5BN5CVZNPGL7vGSoPCQiFhA1i2Pu8w4tLsaPwbxSfwlHvHYsCqV6p57pbqEfOfskDPe_bpmuu241kooxpjs2k9kwVWrm2Un2Wey2J6b7X2X7KX0v2q6TqsvZFdq1dVNLMjTX4zzGpuAI9gymkhhY8ZisseJOox0gA2MOAeYshmpxTBDhskCRUcR7WOGRCPk6KtuoHkdsdyuaY5mShtz66cK4aYEOnt715SZuoiBnl6fNyn7UANzpW7WJs4m3tX0AacaavEh0QP6GxP10-BtSfTnV7LjzJjg2-vcJ_9O_9ycnDeXV2cXJ8eXjRWizY3U2hnFJBOwlMPSSMlkq4WQSkH9UctAiSNumRi0FlzZQYNzSrTGrbjo5Eruk4MX3znifYGU--CThXE0E2BJveSCH2neKl2l_EVqI6YUwfVz9MHEx56zfttQ_6Ghyvx4tS-rAMM78VaJfAbnh45G</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Donadkar, Manoj</creator><creator>Kumar, Brijesh</creator><creator>Singh, Sanjay Kumar</creator><creator>Chandra, Pradeep</creator><creator>Dangi, Pradeep</creator><creator>Gawai, Mohan</creator><creator>Jackson, Amala</creator><creator>Jasrotia, Nancy</creator><creator>Sharma, Shweta</creator><creator>Chouhan, Vikrant</creator><creator>Patra, M K</creator><creator>Khan, Meraj Haider</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6153-0791</orcidid></search><sort><creationdate>202410</creationdate><title>Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows ( Bos indicus )</title><author>Donadkar, Manoj ; Kumar, Brijesh ; Singh, Sanjay Kumar ; Chandra, Pradeep ; Dangi, Pradeep ; Gawai, Mohan ; Jackson, Amala ; Jasrotia, Nancy ; Sharma, Shweta ; Chouhan, Vikrant ; Patra, M K ; Khan, Meraj Haider</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c226t-399fa40302e83d8a33036922344e00360e4251c02d99214cd9eff426afb1273b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Bone Morphogenetic Protein 15 - genetics</topic><topic>Bone Morphogenetic Protein 15 - pharmacology</topic><topic>Cattle</topic><topic>Female</topic><topic>Fertilization in Vitro - methods</topic><topic>Fertilization in Vitro - veterinary</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>Growth Differentiation Factor 9 - genetics</topic><topic>Growth Differentiation Factor 9 - pharmacology</topic><topic>Oocyte Retrieval - methods</topic><topic>Oocyte Retrieval - veterinary</topic><topic>Oocytes - cytology</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - physiology</topic><topic>Ovarian Follicle - drug effects</topic><topic>Proliferating Cell Nuclear Antigen - genetics</topic><topic>Proliferating Cell Nuclear Antigen - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Donadkar, Manoj</creatorcontrib><creatorcontrib>Kumar, Brijesh</creatorcontrib><creatorcontrib>Singh, Sanjay Kumar</creatorcontrib><creatorcontrib>Chandra, Pradeep</creatorcontrib><creatorcontrib>Dangi, Pradeep</creatorcontrib><creatorcontrib>Gawai, Mohan</creatorcontrib><creatorcontrib>Jackson, Amala</creatorcontrib><creatorcontrib>Jasrotia, Nancy</creatorcontrib><creatorcontrib>Sharma, Shweta</creatorcontrib><creatorcontrib>Chouhan, Vikrant</creatorcontrib><creatorcontrib>Patra, M K</creatorcontrib><creatorcontrib>Khan, Meraj Haider</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Zygote (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Donadkar, Manoj</au><au>Kumar, Brijesh</au><au>Singh, Sanjay Kumar</au><au>Chandra, Pradeep</au><au>Dangi, Pradeep</au><au>Gawai, Mohan</au><au>Jackson, Amala</au><au>Jasrotia, Nancy</au><au>Sharma, Shweta</au><au>Chouhan, Vikrant</au><au>Patra, M K</au><au>Khan, Meraj Haider</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows ( Bos indicus )</atitle><jtitle>Zygote (Cambridge)</jtitle><addtitle>Zygote</addtitle><date>2024-10</date><risdate>2024</risdate><volume>32</volume><issue>5</issue><spage>376</spage><epage>385</epage><pages>376-385</pages><issn>0967-1994</issn><issn>1469-8730</issn><eissn>1469-8730</eissn><abstract>The study was conducted on indigenous Tharparkar cow (
) to evaluate FSH stimulation on follicular attributes, oocyte recovery and morpho-molecular developmental competence parameters concerning oocyte quality. A total of 20 OPU sessions were performed, which included 10 sessions in each FSH stimulated at the dose of 130 µg divided into four sub-doses and non-stimulated. Findings on the size of follicles having ≥6 mm showed a significantly higher, however an opposite trend was observed in the case of smaller sized follicle (<6 mm) between stimulated and non-stimulated respectively. The stimulated cows had a significantly higher number as well as the percentage of oocytes of Grade A, having a diameter ≥120 µm and BCB
as compared to the non-stimulated cows. The relative mRNA expression profile of GDF9, BMP15, PCNA and BCL-2 genes was higher and BAX was lower in the FSH-stimulated cow. These results indicated that FSH stimulation before OPU in
cows has a significant impact on follicle size, oocyte yield, recovery, and their quality with respect to COC's, diameter and BCB
oocytes. Further, a significant increase in the relative mRNA expression levels of GDF9, BMP15 and PCNA genes in the FSH-stimulated group suggests that FSH plays a key role in modulating the expression of these important candidate genes and thus influencing oocyte quality. The higher mRNA expression of BCL-2 genes and concomitantly lower expression of BAX gene in FSH Stimulated cows indicates the protective role of these genes and preventing programmed cell death and thus promoting cell survival, quality and embryo development.</abstract><cop>England</cop><pmid>39470002</pmid><doi>10.1017/S0967199424000376</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-6153-0791</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0967-1994 |
| ispartof | Zygote (Cambridge), 2024-10, Vol.32 (5), p.376-385 |
| issn | 0967-1994 1469-8730 1469-8730 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3121591649 |
| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Animals Bone Morphogenetic Protein 15 - genetics Bone Morphogenetic Protein 15 - pharmacology Cattle Female Fertilization in Vitro - methods Fertilization in Vitro - veterinary Follicle Stimulating Hormone - pharmacology Growth Differentiation Factor 9 - genetics Growth Differentiation Factor 9 - pharmacology Oocyte Retrieval - methods Oocyte Retrieval - veterinary Oocytes - cytology Oocytes - drug effects Oocytes - physiology Ovarian Follicle - drug effects Proliferating Cell Nuclear Antigen - genetics Proliferating Cell Nuclear Antigen - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows ( Bos indicus ) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T04%3A48%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Morpho-molecular%20evaluation%20for%20developmental%20competence%20of%20oocytes%20retrieved%20through%20transvaginal%20ovum%20pick-up%20from%20FSH-stimulated%20Tharparkar%20donor%20cows%20(%20Bos%20indicus%20)&rft.jtitle=Zygote%20(Cambridge)&rft.au=Donadkar,%20Manoj&rft.date=2024-10&rft.volume=32&rft.issue=5&rft.spage=376&rft.epage=385&rft.pages=376-385&rft.issn=0967-1994&rft.eissn=1469-8730&rft_id=info:doi/10.1017/S0967199424000376&rft_dat=%3Cproquest_cross%3E3121591649%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3121591649&rft_id=info:pmid/39470002&rfr_iscdi=true |