Comprehensive characterization of gibberellin oxidase gene family in Brassica napus reveals BnGA2ox15 involved in hormone signaling and response to drought stress

Brassica napus is a well-known allopolyploid oil crop with high commercial potential. Gibberellin oxidase (GAox) is an essential enzyme that activates gibberellins, which regulate plant growth, and development, and have a significant impact on plant responses to abiotic stress. However, the comprehe...

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Veröffentlicht in:International journal of biological macromolecules 2024-12, Vol.282 (Pt 2), p.136822, Article 136822
Hauptverfasser: Qin, Tongjun, Huang, Qian, Li, Juanjuan, Ayyaz, Ahsan, Farooq, Muhammad Ahsan, Chen, Weiqi, Zhou, Yingying, Wu, Xiaofen, Ali, Basharat, Zhou, Weijun
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container_issue Pt 2
container_start_page 136822
container_title International journal of biological macromolecules
container_volume 282
creator Qin, Tongjun
Huang, Qian
Li, Juanjuan
Ayyaz, Ahsan
Farooq, Muhammad Ahsan
Chen, Weiqi
Zhou, Yingying
Wu, Xiaofen
Ali, Basharat
Zhou, Weijun
description Brassica napus is a well-known allopolyploid oil crop with high commercial potential. Gibberellin oxidase (GAox) is an essential enzyme that activates gibberellins, which regulate plant growth, and development, and have a significant impact on plant responses to abiotic stress. However, the comprehensive understanding of GAox genes and their evolution in Brassica plants remains elusive. Using advanced bioinformatics tools, this study identified 125 candidate GAox genes from the whole genomes of three key Brassica species. This study also investigated sequence characteristics, conserved motifs, exon/intron structures, cis-acting elements, syntenic analysis, duplication events and expression patterns. Subcellular localization analysis showed that the BnGA2ox14 and BnGA2ox15 proteins are located in the nucleus, whereas BnGA2ox26 is specifically localized to the chloroplast. Yeast one-hybrid and dual-luciferase assays demonstrated that MYELOCYTOMATOSIS 4 (BnMYC4) and ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR (BnAIB) bind to the BnGA2ox15 promoter and activate its transcription. Molecular docking analysis further elucidated their interaction structures and identified potential binding sites. Roots transformations show that overexpression of BnGA2ox15 increased sensitivity to PEG-6000 treatment in rapeseed. In brief, this study reveals that BnGA2ox15 is a downstream target in JA and ABA signaling pathways, functioning as a negative regulator in response to drought stress. •A total of 125 GAox genes were identified in three Bassica plants.•BnGA2ox15 is downstream target responded JA and ABA treatment and signaling.•BnMYC4 and BnAIB could bind to and activate BnGA2ox15 promoter.•Overexpression of BnGA2ox15 reduced drought tolerance.
doi_str_mv 10.1016/j.ijbiomac.2024.136822
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Gibberellin oxidase (GAox) is an essential enzyme that activates gibberellins, which regulate plant growth, and development, and have a significant impact on plant responses to abiotic stress. However, the comprehensive understanding of GAox genes and their evolution in Brassica plants remains elusive. Using advanced bioinformatics tools, this study identified 125 candidate GAox genes from the whole genomes of three key Brassica species. This study also investigated sequence characteristics, conserved motifs, exon/intron structures, cis-acting elements, syntenic analysis, duplication events and expression patterns. Subcellular localization analysis showed that the BnGA2ox14 and BnGA2ox15 proteins are located in the nucleus, whereas BnGA2ox26 is specifically localized to the chloroplast. Yeast one-hybrid and dual-luciferase assays demonstrated that MYELOCYTOMATOSIS 4 (BnMYC4) and ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR (BnAIB) bind to the BnGA2ox15 promoter and activate its transcription. Molecular docking analysis further elucidated their interaction structures and identified potential binding sites. Roots transformations show that overexpression of BnGA2ox15 increased sensitivity to PEG-6000 treatment in rapeseed. In brief, this study reveals that BnGA2ox15 is a downstream target in JA and ABA signaling pathways, functioning as a negative regulator in response to drought stress. •A total of 125 GAox genes were identified in three Bassica plants.•BnGA2ox15 is downstream target responded JA and ABA treatment and signaling.•BnMYC4 and BnAIB could bind to and activate BnGA2ox15 promoter.•Overexpression of BnGA2ox15 reduced drought tolerance.</description><identifier>ISSN: 0141-8130</identifier><identifier>ISSN: 1879-0003</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2024.136822</identifier><identifier>PMID: 39447790</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>BnGA2ox15 ; Brassica napus - enzymology ; Brassica napus - genetics ; Droughts ; Gene Expression Regulation, Plant ; Gibberellin oxidase ; Hormone cross-talk ; Mixed Function Oxygenases - chemistry ; Mixed Function Oxygenases - genetics ; Mixed Function Oxygenases - metabolism ; Molecular Docking Simulation ; Multigene Family ; Phylogeny ; Plant Growth Regulators - metabolism ; Plant Growth Regulators - pharmacology ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Promoter Regions, Genetic - genetics ; Rapeseed ; Signal Transduction ; Stress, Physiological - genetics ; Transgenic roots</subject><ispartof>International journal of biological macromolecules, 2024-12, Vol.282 (Pt 2), p.136822, Article 136822</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. 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Gibberellin oxidase (GAox) is an essential enzyme that activates gibberellins, which regulate plant growth, and development, and have a significant impact on plant responses to abiotic stress. However, the comprehensive understanding of GAox genes and their evolution in Brassica plants remains elusive. Using advanced bioinformatics tools, this study identified 125 candidate GAox genes from the whole genomes of three key Brassica species. This study also investigated sequence characteristics, conserved motifs, exon/intron structures, cis-acting elements, syntenic analysis, duplication events and expression patterns. Subcellular localization analysis showed that the BnGA2ox14 and BnGA2ox15 proteins are located in the nucleus, whereas BnGA2ox26 is specifically localized to the chloroplast. Yeast one-hybrid and dual-luciferase assays demonstrated that MYELOCYTOMATOSIS 4 (BnMYC4) and ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR (BnAIB) bind to the BnGA2ox15 promoter and activate its transcription. Molecular docking analysis further elucidated their interaction structures and identified potential binding sites. Roots transformations show that overexpression of BnGA2ox15 increased sensitivity to PEG-6000 treatment in rapeseed. 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Gibberellin oxidase (GAox) is an essential enzyme that activates gibberellins, which regulate plant growth, and development, and have a significant impact on plant responses to abiotic stress. However, the comprehensive understanding of GAox genes and their evolution in Brassica plants remains elusive. Using advanced bioinformatics tools, this study identified 125 candidate GAox genes from the whole genomes of three key Brassica species. This study also investigated sequence characteristics, conserved motifs, exon/intron structures, cis-acting elements, syntenic analysis, duplication events and expression patterns. Subcellular localization analysis showed that the BnGA2ox14 and BnGA2ox15 proteins are located in the nucleus, whereas BnGA2ox26 is specifically localized to the chloroplast. Yeast one-hybrid and dual-luciferase assays demonstrated that MYELOCYTOMATOSIS 4 (BnMYC4) and ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR (BnAIB) bind to the BnGA2ox15 promoter and activate its transcription. Molecular docking analysis further elucidated their interaction structures and identified potential binding sites. Roots transformations show that overexpression of BnGA2ox15 increased sensitivity to PEG-6000 treatment in rapeseed. In brief, this study reveals that BnGA2ox15 is a downstream target in JA and ABA signaling pathways, functioning as a negative regulator in response to drought stress. •A total of 125 GAox genes were identified in three Bassica plants.•BnGA2ox15 is downstream target responded JA and ABA treatment and signaling.•BnMYC4 and BnAIB could bind to and activate BnGA2ox15 promoter.•Overexpression of BnGA2ox15 reduced drought tolerance.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>39447790</pmid><doi>10.1016/j.ijbiomac.2024.136822</doi></addata></record>
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subjects BnGA2ox15
Brassica napus - enzymology
Brassica napus - genetics
Droughts
Gene Expression Regulation, Plant
Gibberellin oxidase
Hormone cross-talk
Mixed Function Oxygenases - chemistry
Mixed Function Oxygenases - genetics
Mixed Function Oxygenases - metabolism
Molecular Docking Simulation
Multigene Family
Phylogeny
Plant Growth Regulators - metabolism
Plant Growth Regulators - pharmacology
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Promoter Regions, Genetic - genetics
Rapeseed
Signal Transduction
Stress, Physiological - genetics
Transgenic roots
title Comprehensive characterization of gibberellin oxidase gene family in Brassica napus reveals BnGA2ox15 involved in hormone signaling and response to drought stress
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